Strain Fitness in Pseudomonas fluorescens SBW25 around PFLU_RS14290

Experiment: succinate (C); with MOPS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS14285 and PFLU_RS14290 are separated by 78 nucleotidesPFLU_RS14290 and PFLU_RS14295 overlap by 4 nucleotides PFLU_RS14285: PFLU_RS14285 - ABC transporter substrate-binding protein, at 3,202,352 to 3,203,320 _RS14285 PFLU_RS14290: PFLU_RS14290 - response regulator, at 3,203,399 to 3,203,839 _RS14290 PFLU_RS14295: PFLU_RS14295 - PAS domain S-box protein, at 3,203,836 to 3,206,151 _RS14295 Position (kb) 3203 3204Strain fitness (log2 ratio) -2 -1 0 1at 3202.529 kb on + strand, within PFLU_RS14285at 3202.530 kb on - strand, within PFLU_RS14285at 3202.530 kb on - strand, within PFLU_RS14285at 3202.547 kb on - strand, within PFLU_RS14285at 3202.592 kb on - strand, within PFLU_RS14285at 3202.592 kb on - strand, within PFLU_RS14285at 3202.592 kb on - strand, within PFLU_RS14285at 3202.712 kb on + strand, within PFLU_RS14285at 3202.713 kb on - strand, within PFLU_RS14285at 3202.713 kb on - strand, within PFLU_RS14285at 3202.713 kb on - strand, within PFLU_RS14285at 3202.713 kb on - strand, within PFLU_RS14285at 3202.724 kb on + strand, within PFLU_RS14285at 3202.725 kb on - strand, within PFLU_RS14285at 3202.850 kb on + strand, within PFLU_RS14285at 3202.851 kb on - strand, within PFLU_RS14285at 3202.907 kb on + strand, within PFLU_RS14285at 3202.907 kb on + strand, within PFLU_RS14285at 3202.925 kb on + strand, within PFLU_RS14285at 3202.926 kb on - strand, within PFLU_RS14285at 3202.926 kb on - strand, within PFLU_RS14285at 3202.927 kb on + strand, within PFLU_RS14285at 3202.982 kb on + strand, within PFLU_RS14285at 3202.982 kb on + strand, within PFLU_RS14285at 3203.009 kb on + strand, within PFLU_RS14285at 3203.009 kb on + strand, within PFLU_RS14285at 3203.009 kb on + strand, within PFLU_RS14285at 3203.010 kb on - strand, within PFLU_RS14285at 3203.013 kb on - strand, within PFLU_RS14285at 3203.081 kb on - strand, within PFLU_RS14285at 3203.389 kb on + strandat 3203.390 kb on - strandat 3203.452 kb on - strand, within PFLU_RS14290at 3203.601 kb on + strand, within PFLU_RS14290at 3203.602 kb on - strand, within PFLU_RS14290at 3203.744 kb on + strand, within PFLU_RS14290at 3203.745 kb on - strand, within PFLU_RS14290at 3203.745 kb on - strand, within PFLU_RS14290at 3203.872 kb on + strandat 3204.016 kb on + strandat 3204.017 kb on - strandat 3204.194 kb on + strand, within PFLU_RS14295at 3204.194 kb on + strand, within PFLU_RS14295at 3204.194 kb on + strand, within PFLU_RS14295at 3204.195 kb on - strand, within PFLU_RS14295at 3204.195 kb on - strand, within PFLU_RS14295at 3204.195 kb on - strand, within PFLU_RS14295at 3204.563 kb on + strand, within PFLU_RS14295at 3204.563 kb on + strand, within PFLU_RS14295at 3204.563 kb on + strand, within PFLU_RS14295at 3204.564 kb on - strand, within PFLU_RS14295at 3204.564 kb on - strand, within PFLU_RS14295at 3204.831 kb on + strand, within PFLU_RS14295

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Per-strain Table

Position Strand Gene LocusTag Fraction succinate (C); with MOPS
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3,202,529 + PFLU_RS14285 0.18 -0.4
3,202,530 - PFLU_RS14285 0.18 +0.1
3,202,530 - PFLU_RS14285 0.18 -0.2
3,202,547 - PFLU_RS14285 0.20 -0.4
3,202,592 - PFLU_RS14285 0.25 +0.5
3,202,592 - PFLU_RS14285 0.25 -1.5
3,202,592 - PFLU_RS14285 0.25 +1.1
3,202,712 + PFLU_RS14285 0.37 -2.4
3,202,713 - PFLU_RS14285 0.37 +0.1
3,202,713 - PFLU_RS14285 0.37 +0.3
3,202,713 - PFLU_RS14285 0.37 -0.6
3,202,713 - PFLU_RS14285 0.37 +1.1
3,202,724 + PFLU_RS14285 0.38 +0.4
3,202,725 - PFLU_RS14285 0.38 +1.6
3,202,850 + PFLU_RS14285 0.51 +0.1
3,202,851 - PFLU_RS14285 0.51 -0.3
3,202,907 + PFLU_RS14285 0.57 +0.2
3,202,907 + PFLU_RS14285 0.57 +1.2
3,202,925 + PFLU_RS14285 0.59 +0.8
3,202,926 - PFLU_RS14285 0.59 -0.1
3,202,926 - PFLU_RS14285 0.59 +0.7
3,202,927 + PFLU_RS14285 0.59 -0.2
3,202,982 + PFLU_RS14285 0.65 +1.3
3,202,982 + PFLU_RS14285 0.65 -0.0
3,203,009 + PFLU_RS14285 0.68 -0.1
3,203,009 + PFLU_RS14285 0.68 +0.4
3,203,009 + PFLU_RS14285 0.68 +1.4
3,203,010 - PFLU_RS14285 0.68 -1.2
3,203,013 - PFLU_RS14285 0.68 -0.3
3,203,081 - PFLU_RS14285 0.75 +0.5
3,203,389 + +1.3
3,203,390 - -0.2
3,203,452 - PFLU_RS14290 0.12 -0.2
3,203,601 + PFLU_RS14290 0.46 +0.3
3,203,602 - PFLU_RS14290 0.46 +1.0
3,203,744 + PFLU_RS14290 0.78 -2.3
3,203,745 - PFLU_RS14290 0.78 +1.1
3,203,745 - PFLU_RS14290 0.78 +0.2
3,203,872 + -0.2
3,204,016 + +0.2
3,204,017 - -1.5
3,204,194 + PFLU_RS14295 0.15 -0.4
3,204,194 + PFLU_RS14295 0.15 +1.1
3,204,194 + PFLU_RS14295 0.15 +0.1
3,204,195 - PFLU_RS14295 0.16 -0.4
3,204,195 - PFLU_RS14295 0.16 -1.3
3,204,195 - PFLU_RS14295 0.16 -0.3
3,204,563 + PFLU_RS14295 0.31 +0.5
3,204,563 + PFLU_RS14295 0.31 -0.4
3,204,563 + PFLU_RS14295 0.31 -0.9
3,204,564 - PFLU_RS14295 0.31 -0.3
3,204,564 - PFLU_RS14295 0.31 +0.1
3,204,831 + PFLU_RS14295 0.43 +0.2

Or see this region's nucleotide sequence