Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4253 and EX28DRAFT_4254 are separated by 18 nucleotides EX28DRAFT_4254 and EX28DRAFT_4255 are separated by 65 nucleotides EX28DRAFT_4255 and EX28DRAFT_4256 are separated by 0 nucleotides EX28DRAFT_4256 and EX28DRAFT_4257 are separated by 81 nucleotides
EX28DRAFT_4253: EX28DRAFT_4253 - Phage-related lysozyme (muraminidase), at 59,860 to 60,309
_4253
EX28DRAFT_4254: EX28DRAFT_4254 - ribonuclease, at 60,328 to 60,810
_4254
EX28DRAFT_4255: EX28DRAFT_4255 - hypothetical protein, at 60,876 to 61,283
_4255
EX28DRAFT_4256: EX28DRAFT_4256 - hypothetical protein, at 61,284 to 61,574
_4256
EX28DRAFT_4257: EX28DRAFT_4257 - Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain, at 61,656 to 62,261
_4257
Position (kb)
60
61
62 Strain fitness (log2 ratio)
-2
-1
0
1 at 59.880 kb on + strand at 59.881 kb on - strand at 60.238 kb on - strand, within EX28DRAFT_4253 at 60.296 kb on + strand at 60.304 kb on - strand at 60.307 kb on + strand at 60.426 kb on - strand, within EX28DRAFT_4254 at 60.426 kb on - strand, within EX28DRAFT_4254 at 60.460 kb on - strand, within EX28DRAFT_4254 at 60.508 kb on - strand, within EX28DRAFT_4254 at 60.539 kb on - strand, within EX28DRAFT_4254 at 60.540 kb on + strand, within EX28DRAFT_4254 at 60.541 kb on - strand, within EX28DRAFT_4254 at 60.643 kb on + strand, within EX28DRAFT_4254 at 60.733 kb on + strand, within EX28DRAFT_4254 at 60.775 kb on + strand at 60.776 kb on - strand at 60.776 kb on - strand at 60.776 kb on - strand at 60.776 kb on - strand at 60.783 kb on + strand at 60.784 kb on - strand at 60.808 kb on + strand at 60.809 kb on - strand at 60.809 kb on - strand at 60.830 kb on + strand at 60.830 kb on + strand at 60.875 kb on + strand at 60.875 kb on + strand at 60.876 kb on - strand at 60.876 kb on - strand at 60.889 kb on - strand at 60.998 kb on + strand, within EX28DRAFT_4255 at 60.999 kb on - strand, within EX28DRAFT_4255 at 60.999 kb on - strand, within EX28DRAFT_4255 at 61.010 kb on + strand, within EX28DRAFT_4255 at 61.079 kb on - strand, within EX28DRAFT_4255 at 61.119 kb on - strand, within EX28DRAFT_4255 at 61.136 kb on + strand, within EX28DRAFT_4255 at 61.137 kb on - strand, within EX28DRAFT_4255 at 61.295 kb on - strand at 61.369 kb on - strand, within EX28DRAFT_4256 at 61.662 kb on - strand at 61.669 kb on + strand at 61.740 kb on + strand, within EX28DRAFT_4257 at 61.740 kb on + strand, within EX28DRAFT_4257 at 61.741 kb on - strand, within EX28DRAFT_4257 at 61.846 kb on + strand, within EX28DRAFT_4257 at 61.933 kb on - strand, within EX28DRAFT_4257 at 61.933 kb on - strand, within EX28DRAFT_4257 at 61.968 kb on - strand, within EX28DRAFT_4257 at 62.007 kb on + strand, within EX28DRAFT_4257 at 62.072 kb on + strand, within EX28DRAFT_4257 at 62.114 kb on + strand, within EX28DRAFT_4257 at 62.130 kb on - strand, within EX28DRAFT_4257
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 59,880 + -2.4 59,881 - +0.0 60,238 - EX28DRAFT_4253 0.84 -0.2 60,296 + +0.1 60,304 - -0.2 60,307 + +0.1 60,426 - EX28DRAFT_4254 0.20 +0.4 60,426 - EX28DRAFT_4254 0.20 +0.2 60,460 - EX28DRAFT_4254 0.27 +0.1 60,508 - EX28DRAFT_4254 0.37 -1.2 60,539 - EX28DRAFT_4254 0.44 +0.4 60,540 + EX28DRAFT_4254 0.44 -1.3 60,541 - EX28DRAFT_4254 0.44 +0.3 60,643 + EX28DRAFT_4254 0.65 +0.1 60,733 + EX28DRAFT_4254 0.84 +0.1 60,775 + +0.1 60,776 - +0.3 60,776 - -2.2 60,776 - -0.4 60,776 - +0.3 60,783 + -0.9 60,784 - +0.1 60,808 + -0.1 60,809 - -1.9 60,809 - +0.2 60,830 + -0.6 60,830 + +0.0 60,875 + +0.0 60,875 + -1.0 60,876 - +0.8 60,876 - -0.6 60,889 - +0.1 60,998 + EX28DRAFT_4255 0.30 -0.7 60,999 - EX28DRAFT_4255 0.30 -0.9 60,999 - EX28DRAFT_4255 0.30 -0.4 61,010 + EX28DRAFT_4255 0.33 +0.1 61,079 - EX28DRAFT_4255 0.50 -0.5 61,119 - EX28DRAFT_4255 0.60 -1.0 61,136 + EX28DRAFT_4255 0.64 +0.1 61,137 - EX28DRAFT_4255 0.64 +0.6 61,295 - -0.0 61,369 - EX28DRAFT_4256 0.29 -0.3 61,662 - +1.2 61,669 + +0.8 61,740 + EX28DRAFT_4257 0.14 +1.1 61,740 + EX28DRAFT_4257 0.14 -0.7 61,741 - EX28DRAFT_4257 0.14 +0.6 61,846 + EX28DRAFT_4257 0.31 -0.1 61,933 - EX28DRAFT_4257 0.46 -0.1 61,933 - EX28DRAFT_4257 0.46 +1.2 61,968 - EX28DRAFT_4257 0.51 +0.1 62,007 + EX28DRAFT_4257 0.58 -0.4 62,072 + EX28DRAFT_4257 0.69 -1.5 62,114 + EX28DRAFT_4257 0.76 -2.0 62,130 - EX28DRAFT_4257 0.78 +0.5
Or see this region's nucleotide sequence