Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_4020

Experiment: MS_media with arsenate 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_4018 and EX28DRAFT_4019 are separated by 90 nucleotidesEX28DRAFT_4019 and EX28DRAFT_4020 are separated by 151 nucleotidesEX28DRAFT_4020 and EX28DRAFT_4021 are separated by 0 nucleotides EX28DRAFT_4018: EX28DRAFT_4018 - Flavodoxins, at 228,679 to 229,128 _4018 EX28DRAFT_4019: EX28DRAFT_4019 - Transcriptional regulators, at 229,219 to 229,677 _4019 EX28DRAFT_4020: EX28DRAFT_4020 - aspartate-ammonia ligase (EC 6.3.1.1), at 229,829 to 230,821 _4020 EX28DRAFT_4021: EX28DRAFT_4021 - Uncharacterized protein containing a von Willebrand factor type A (vWA) domain, at 230,822 to 232,273 _4021 Position (kb) 229 230 231Strain fitness (log2 ratio) -3 -2 -1 0 1at 228.920 kb on + strand, within EX28DRAFT_4018at 228.921 kb on - strand, within EX28DRAFT_4018at 228.922 kb on + strand, within EX28DRAFT_4018at 228.922 kb on + strand, within EX28DRAFT_4018at 229.145 kb on + strandat 229.163 kb on - strandat 229.163 kb on - strandat 229.163 kb on - strandat 229.163 kb on - strandat 229.163 kb on - strandat 229.234 kb on - strandat 229.308 kb on + strand, within EX28DRAFT_4019at 229.308 kb on + strand, within EX28DRAFT_4019at 229.308 kb on + strand, within EX28DRAFT_4019at 229.308 kb on + strand, within EX28DRAFT_4019at 229.308 kb on + strand, within EX28DRAFT_4019at 229.308 kb on + strand, within EX28DRAFT_4019at 229.309 kb on - strand, within EX28DRAFT_4019at 229.309 kb on - strand, within EX28DRAFT_4019at 229.312 kb on + strand, within EX28DRAFT_4019at 229.312 kb on + strand, within EX28DRAFT_4019at 229.313 kb on - strand, within EX28DRAFT_4019at 229.313 kb on - strand, within EX28DRAFT_4019at 229.351 kb on - strand, within EX28DRAFT_4019at 229.354 kb on - strand, within EX28DRAFT_4019at 229.380 kb on - strand, within EX28DRAFT_4019at 229.380 kb on - strand, within EX28DRAFT_4019at 229.383 kb on - strand, within EX28DRAFT_4019at 229.803 kb on - strandat 229.823 kb on + strandat 229.823 kb on + strandat 229.823 kb on + strandat 229.823 kb on + strandat 229.824 kb on - strandat 229.824 kb on - strandat 229.827 kb on + strandat 229.827 kb on + strandat 229.827 kb on + strandat 229.842 kb on - strandat 229.842 kb on - strandat 229.873 kb on - strandat 229.966 kb on + strand, within EX28DRAFT_4020at 229.967 kb on - strand, within EX28DRAFT_4020at 230.105 kb on + strand, within EX28DRAFT_4020at 230.166 kb on - strand, within EX28DRAFT_4020at 230.406 kb on + strand, within EX28DRAFT_4020at 230.416 kb on - strand, within EX28DRAFT_4020at 230.416 kb on - strand, within EX28DRAFT_4020at 230.416 kb on - strand, within EX28DRAFT_4020at 230.424 kb on + strand, within EX28DRAFT_4020at 230.424 kb on + strand, within EX28DRAFT_4020at 230.428 kb on + strand, within EX28DRAFT_4020at 230.428 kb on + strand, within EX28DRAFT_4020at 230.480 kb on + strand, within EX28DRAFT_4020at 230.481 kb on - strand, within EX28DRAFT_4020at 230.481 kb on - strand, within EX28DRAFT_4020at 230.749 kb on - strandat 230.793 kb on + strandat 230.819 kb on + strandat 230.878 kb on + strandat 230.879 kb on - strandat 230.879 kb on - strandat 231.058 kb on + strand, within EX28DRAFT_4021at 231.059 kb on - strand, within EX28DRAFT_4021at 231.165 kb on + strand, within EX28DRAFT_4021at 231.194 kb on - strand, within EX28DRAFT_4021at 231.292 kb on + strand, within EX28DRAFT_4021at 231.439 kb on - strand, within EX28DRAFT_4021at 231.669 kb on + strand, within EX28DRAFT_4021at 231.787 kb on + strand, within EX28DRAFT_4021at 231.788 kb on - strand, within EX28DRAFT_4021at 231.788 kb on - strand, within EX28DRAFT_4021

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Per-strain Table

Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM
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228,920 + EX28DRAFT_4018 0.54 +1.4
228,921 - EX28DRAFT_4018 0.54 -1.6
228,922 + EX28DRAFT_4018 0.54 -2.0
228,922 + EX28DRAFT_4018 0.54 -2.4
229,145 + -0.1
229,163 - +0.3
229,163 - +0.1
229,163 - +0.2
229,163 - -0.1
229,163 - -1.4
229,234 - -0.1
229,308 + EX28DRAFT_4019 0.19 -1.1
229,308 + EX28DRAFT_4019 0.19 -0.2
229,308 + EX28DRAFT_4019 0.19 -0.6
229,308 + EX28DRAFT_4019 0.19 -3.3
229,308 + EX28DRAFT_4019 0.19 -1.0
229,308 + EX28DRAFT_4019 0.19 -0.6
229,309 - EX28DRAFT_4019 0.20 -0.1
229,309 - EX28DRAFT_4019 0.20 +0.0
229,312 + EX28DRAFT_4019 0.20 -2.8
229,312 + EX28DRAFT_4019 0.20 -1.1
229,313 - EX28DRAFT_4019 0.20 -1.2
229,313 - EX28DRAFT_4019 0.20 -0.5
229,351 - EX28DRAFT_4019 0.29 +0.4
229,354 - EX28DRAFT_4019 0.29 -2.3
229,380 - EX28DRAFT_4019 0.35 -2.5
229,380 - EX28DRAFT_4019 0.35 -0.8
229,383 - EX28DRAFT_4019 0.36 -0.5
229,803 - -0.3
229,823 + -1.1
229,823 + +0.5
229,823 + -0.2
229,823 + +0.3
229,824 - +0.2
229,824 - +0.2
229,827 + +0.4
229,827 + +0.1
229,827 + +0.2
229,842 - -0.8
229,842 - -0.3
229,873 - -0.1
229,966 + EX28DRAFT_4020 0.14 +0.4
229,967 - EX28DRAFT_4020 0.14 +1.6
230,105 + EX28DRAFT_4020 0.28 +0.1
230,166 - EX28DRAFT_4020 0.34 +1.1
230,406 + EX28DRAFT_4020 0.58 +0.5
230,416 - EX28DRAFT_4020 0.59 +0.1
230,416 - EX28DRAFT_4020 0.59 -0.6
230,416 - EX28DRAFT_4020 0.59 +1.2
230,424 + EX28DRAFT_4020 0.60 +0.5
230,424 + EX28DRAFT_4020 0.60 -0.4
230,428 + EX28DRAFT_4020 0.60 +0.1
230,428 + EX28DRAFT_4020 0.60 +0.7
230,480 + EX28DRAFT_4020 0.66 +0.4
230,481 - EX28DRAFT_4020 0.66 +1.3
230,481 - EX28DRAFT_4020 0.66 +0.3
230,749 - +0.5
230,793 + +0.2
230,819 + -1.3
230,878 + +0.5
230,879 - +0.1
230,879 - +0.7
231,058 + EX28DRAFT_4021 0.16 +0.2
231,059 - EX28DRAFT_4021 0.16 +0.3
231,165 + EX28DRAFT_4021 0.24 -1.2
231,194 - EX28DRAFT_4021 0.26 +0.0
231,292 + EX28DRAFT_4021 0.32 -0.3
231,439 - EX28DRAFT_4021 0.42 +0.6
231,669 + EX28DRAFT_4021 0.58 -0.3
231,787 + EX28DRAFT_4021 0.66 +0.2
231,788 - EX28DRAFT_4021 0.67 -0.1
231,788 - EX28DRAFT_4021 0.67 +0.8

Or see this region's nucleotide sequence