Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3400 and EX28DRAFT_3401 are separated by 190 nucleotides EX28DRAFT_3401 and EX28DRAFT_3402 overlap by 7 nucleotides EX28DRAFT_3402 and EX28DRAFT_3403 are separated by 324 nucleotides EX28DRAFT_3403 and EX28DRAFT_3404 are separated by 72 nucleotides
EX28DRAFT_3400: EX28DRAFT_3400 - 2',3'-cyclic-nucleotide 2'-phosphodiesterase, at 68,823 to 70,766
_3400
EX28DRAFT_3401: EX28DRAFT_3401 - 3'(2'),5'-bisphosphate nucleotidase, bacterial, at 70,957 to 71,697
_3401
EX28DRAFT_3402: EX28DRAFT_3402 - conserved hypothetical protein YtfJ-family, TIGR01626, at 71,691 to 72,248
_3402
EX28DRAFT_3403: EX28DRAFT_3403 - Protein of unknown function (DUF1107), at 72,573 to 72,779
_3403
EX28DRAFT_3404: EX28DRAFT_3404 - Hemolysins and related proteins containing CBS domains, at 72,852 to 74,189
_3404
Position (kb) 71
72
73 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 70.692 kb on - strand at 70.701 kb on + strand at 70.754 kb on + strand at 70.804 kb on + strand at 70.804 kb on + strand at 70.808 kb on + strand at 70.809 kb on - strand at 70.903 kb on - strand at 70.908 kb on - strand at 71.452 kb on + strand, within EX28DRAFT_3401 at 71.452 kb on + strand, within EX28DRAFT_3401 at 71.644 kb on + strand at 71.646 kb on + strand at 71.646 kb on + strand at 71.692 kb on + strand at 71.693 kb on - strand at 72.050 kb on + strand, within EX28DRAFT_3402 at 72.051 kb on - strand, within EX28DRAFT_3402 at 72.051 kb on - strand, within EX28DRAFT_3402 at 72.089 kb on + strand, within EX28DRAFT_3402 at 72.103 kb on + strand, within EX28DRAFT_3402 at 72.104 kb on - strand, within EX28DRAFT_3402 at 72.104 kb on - strand, within EX28DRAFT_3402 at 72.108 kb on - strand, within EX28DRAFT_3402 at 72.114 kb on - strand, within EX28DRAFT_3402 at 72.233 kb on + strand at 72.233 kb on + strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.240 kb on + strand at 72.299 kb on + strand at 72.299 kb on + strand at 72.300 kb on - strand at 72.300 kb on - strand at 72.300 kb on - strand at 72.305 kb on + strand at 72.305 kb on + strand at 72.306 kb on - strand at 72.364 kb on + strand at 72.364 kb on + strand at 72.365 kb on - strand at 72.388 kb on + strand at 72.454 kb on - strand at 72.474 kb on + strand at 72.485 kb on + strand at 72.485 kb on + strand at 72.486 kb on - strand at 72.486 kb on - strand at 72.488 kb on - strand at 72.551 kb on - strand at 72.567 kb on - strand at 72.591 kb on + strand at 72.592 kb on - strand at 72.592 kb on - strand at 72.615 kb on + strand, within EX28DRAFT_3403 at 72.616 kb on - strand, within EX28DRAFT_3403 at 72.645 kb on + strand, within EX28DRAFT_3403 at 72.645 kb on + strand, within EX28DRAFT_3403 at 72.645 kb on + strand, within EX28DRAFT_3403 at 72.646 kb on - strand, within EX28DRAFT_3403 at 72.646 kb on - strand, within EX28DRAFT_3403 at 72.646 kb on - strand, within EX28DRAFT_3403 at 72.646 kb on - strand, within EX28DRAFT_3403 at 72.790 kb on - strand at 72.796 kb on + strand at 72.897 kb on + strand at 73.017 kb on - strand, within EX28DRAFT_3404 at 73.025 kb on + strand, within EX28DRAFT_3404 at 73.025 kb on + strand, within EX28DRAFT_3404 at 73.035 kb on - strand, within EX28DRAFT_3404 at 73.098 kb on + strand, within EX28DRAFT_3404 at 73.140 kb on - strand, within EX28DRAFT_3404 at 73.224 kb on - strand, within EX28DRAFT_3404 at 73.227 kb on - strand, within EX28DRAFT_3404
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 70,692 - +0.5 70,701 + -2.3 70,754 + -1.3 70,804 + +0.1 70,804 + -0.2 70,808 + -0.2 70,809 - +0.3 70,903 - -0.9 70,908 - -0.7 71,452 + EX28DRAFT_3401 0.67 -2.3 71,452 + EX28DRAFT_3401 0.67 -2.8 71,644 + -0.5 71,646 + -2.1 71,646 + -3.9 71,692 + -2.8 71,693 - -0.2 72,050 + EX28DRAFT_3402 0.64 -0.0 72,051 - EX28DRAFT_3402 0.65 +0.1 72,051 - EX28DRAFT_3402 0.65 -0.2 72,089 + EX28DRAFT_3402 0.71 -0.3 72,103 + EX28DRAFT_3402 0.74 +0.3 72,104 - EX28DRAFT_3402 0.74 +0.1 72,104 - EX28DRAFT_3402 0.74 -0.3 72,108 - EX28DRAFT_3402 0.75 +2.1 72,114 - EX28DRAFT_3402 0.76 -0.0 72,233 + +0.7 72,233 + -0.0 72,237 - +0.3 72,237 - +0.1 72,240 + +0.9 72,299 + +0.4 72,299 + -0.5 72,300 - -2.2 72,300 - -0.4 72,300 - +1.4 72,305 + +0.2 72,305 + -0.1 72,306 - -0.0 72,364 + +0.0 72,364 + +1.4 72,365 - +0.3 72,388 + -0.5 72,454 - +0.3 72,474 + +1.5 72,485 + +0.9 72,485 + +2.3 72,486 - -0.3 72,486 - +0.7 72,488 - -1.4 72,551 - -0.0 72,567 - +0.2 72,591 + -0.1 72,592 - +0.0 72,592 - -0.3 72,615 + EX28DRAFT_3403 0.20 -0.1 72,616 - EX28DRAFT_3403 0.21 -0.2 72,645 + EX28DRAFT_3403 0.35 -0.2 72,645 + EX28DRAFT_3403 0.35 +0.3 72,645 + EX28DRAFT_3403 0.35 +0.4 72,646 - EX28DRAFT_3403 0.35 -0.5 72,646 - EX28DRAFT_3403 0.35 +0.4 72,646 - EX28DRAFT_3403 0.35 -2.3 72,646 - EX28DRAFT_3403 0.35 -0.3 72,790 - -0.0 72,796 + +0.3 72,897 + -0.2 73,017 - EX28DRAFT_3404 0.12 +0.2 73,025 + EX28DRAFT_3404 0.13 -0.6 73,025 + EX28DRAFT_3404 0.13 +0.9 73,035 - EX28DRAFT_3404 0.14 -0.5 73,098 + EX28DRAFT_3404 0.18 -0.5 73,140 - EX28DRAFT_3404 0.22 -0.2 73,224 - EX28DRAFT_3404 0.28 -2.4 73,227 - EX28DRAFT_3404 0.28 -0.4
Or see this region's nucleotide sequence