Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2998 and EX28DRAFT_2999 are separated by 26 nucleotides EX28DRAFT_2999 and EX28DRAFT_3000 are separated by 53 nucleotides EX28DRAFT_3000 and EX28DRAFT_3001 are separated by 225 nucleotides
EX28DRAFT_2998: EX28DRAFT_2998 - A/G-specific DNA-adenine glycosylase (EC 3.2.2.-), at 306,568 to 307,626
_2998
EX28DRAFT_2999: EX28DRAFT_2999 - Fe-S cluster protector protein, at 307,653 to 307,925
_2999
EX28DRAFT_3000: EX28DRAFT_3000 - Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains), at 307,979 to 309,058
_3000
EX28DRAFT_3001: EX28DRAFT_3001 - nucleoside transporter, at 309,284 to 310,540
_3001
Position (kb) 307
308
309
310 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 307.074 kb on - strand, within EX28DRAFT_2998 at 307.074 kb on - strand, within EX28DRAFT_2998 at 307.074 kb on - strand, within EX28DRAFT_2998 at 307.275 kb on + strand, within EX28DRAFT_2998 at 307.275 kb on + strand, within EX28DRAFT_2998 at 307.275 kb on + strand, within EX28DRAFT_2998 at 307.276 kb on - strand, within EX28DRAFT_2998 at 307.276 kb on - strand, within EX28DRAFT_2998 at 307.276 kb on - strand, within EX28DRAFT_2998 at 307.276 kb on - strand, within EX28DRAFT_2998 at 307.276 kb on - strand, within EX28DRAFT_2998 at 307.346 kb on + strand, within EX28DRAFT_2998 at 307.346 kb on + strand, within EX28DRAFT_2998 at 307.346 kb on + strand, within EX28DRAFT_2998 at 307.347 kb on - strand, within EX28DRAFT_2998 at 307.347 kb on - strand, within EX28DRAFT_2998 at 307.348 kb on + strand, within EX28DRAFT_2998 at 307.540 kb on + strand at 307.542 kb on + strand at 307.543 kb on - strand at 307.547 kb on + strand at 307.604 kb on + strand at 307.604 kb on + strand at 307.605 kb on - strand at 307.605 kb on - strand at 307.673 kb on + strand at 307.743 kb on + strand, within EX28DRAFT_2999 at 307.743 kb on + strand, within EX28DRAFT_2999 at 307.905 kb on + strand at 307.906 kb on - strand at 307.908 kb on - strand at 307.908 kb on - strand at 307.908 kb on - strand at 307.924 kb on - strand at 307.952 kb on - strand at 308.128 kb on + strand, within EX28DRAFT_3000 at 308.128 kb on + strand, within EX28DRAFT_3000 at 308.129 kb on - strand, within EX28DRAFT_3000 at 308.129 kb on - strand, within EX28DRAFT_3000 at 308.129 kb on - strand, within EX28DRAFT_3000 at 308.129 kb on - strand, within EX28DRAFT_3000 at 308.129 kb on - strand, within EX28DRAFT_3000 at 308.130 kb on + strand, within EX28DRAFT_3000 at 308.130 kb on + strand, within EX28DRAFT_3000 at 308.164 kb on + strand, within EX28DRAFT_3000 at 308.171 kb on + strand, within EX28DRAFT_3000 at 308.171 kb on + strand, within EX28DRAFT_3000 at 308.171 kb on + strand, within EX28DRAFT_3000 at 308.172 kb on - strand, within EX28DRAFT_3000 at 308.176 kb on + strand, within EX28DRAFT_3000 at 308.180 kb on + strand, within EX28DRAFT_3000 at 308.181 kb on - strand, within EX28DRAFT_3000 at 308.181 kb on - strand, within EX28DRAFT_3000 at 308.228 kb on - strand, within EX28DRAFT_3000 at 308.324 kb on - strand, within EX28DRAFT_3000 at 308.333 kb on + strand, within EX28DRAFT_3000 at 308.334 kb on - strand, within EX28DRAFT_3000 at 308.447 kb on + strand, within EX28DRAFT_3000 at 308.494 kb on + strand, within EX28DRAFT_3000 at 308.548 kb on + strand, within EX28DRAFT_3000 at 308.548 kb on + strand, within EX28DRAFT_3000 at 308.549 kb on - strand, within EX28DRAFT_3000 at 308.552 kb on + strand, within EX28DRAFT_3000 at 308.553 kb on - strand, within EX28DRAFT_3000 at 308.585 kb on + strand, within EX28DRAFT_3000 at 308.586 kb on - strand, within EX28DRAFT_3000 at 308.617 kb on + strand, within EX28DRAFT_3000 at 308.686 kb on - strand, within EX28DRAFT_3000 at 308.753 kb on + strand, within EX28DRAFT_3000 at 308.753 kb on + strand, within EX28DRAFT_3000 at 308.754 kb on - strand, within EX28DRAFT_3000 at 308.967 kb on - strand at 309.012 kb on - strand at 309.017 kb on + strand at 309.017 kb on + strand at 309.017 kb on + strand at 309.017 kb on + strand at 309.018 kb on - strand at 309.044 kb on + strand at 309.044 kb on + strand at 309.044 kb on + strand at 309.194 kb on + strand at 309.194 kb on + strand at 309.194 kb on + strand at 309.194 kb on + strand at 309.194 kb on + strand at 309.194 kb on + strand at 309.195 kb on - strand at 309.195 kb on - strand at 309.195 kb on - strand at 309.195 kb on - strand at 309.195 kb on - strand at 309.195 kb on - strand at 309.195 kb on - strand at 309.195 kb on - strand at 309.195 kb on - strand at 309.195 kb on - strand at 309.195 kb on - strand at 309.195 kb on - strand at 309.225 kb on + strand at 309.293 kb on - strand at 309.353 kb on - strand at 309.365 kb on + strand at 309.365 kb on + strand at 309.365 kb on + strand at 309.366 kb on - strand at 309.366 kb on - strand at 309.366 kb on - strand at 309.367 kb on + strand at 309.367 kb on + strand at 309.367 kb on + strand at 309.368 kb on - strand at 309.368 kb on - strand at 309.497 kb on + strand, within EX28DRAFT_3001 at 309.497 kb on + strand, within EX28DRAFT_3001 at 309.498 kb on - strand, within EX28DRAFT_3001 at 309.498 kb on - strand, within EX28DRAFT_3001 at 309.586 kb on + strand, within EX28DRAFT_3001 at 309.586 kb on + strand, within EX28DRAFT_3001 at 309.599 kb on + strand, within EX28DRAFT_3001 at 309.599 kb on + strand, within EX28DRAFT_3001 at 309.599 kb on + strand, within EX28DRAFT_3001 at 309.599 kb on + strand, within EX28DRAFT_3001 at 309.600 kb on - strand, within EX28DRAFT_3001 at 309.600 kb on - strand, within EX28DRAFT_3001 at 309.600 kb on - strand, within EX28DRAFT_3001 at 309.602 kb on - strand, within EX28DRAFT_3001 at 309.602 kb on - strand, within EX28DRAFT_3001 at 309.602 kb on - strand, within EX28DRAFT_3001 at 309.635 kb on + strand, within EX28DRAFT_3001 at 309.635 kb on + strand, within EX28DRAFT_3001 at 309.635 kb on + strand, within EX28DRAFT_3001 at 309.636 kb on - strand, within EX28DRAFT_3001 at 309.691 kb on + strand, within EX28DRAFT_3001 at 309.692 kb on - strand, within EX28DRAFT_3001 at 309.712 kb on + strand, within EX28DRAFT_3001 at 309.713 kb on - strand, within EX28DRAFT_3001 at 309.761 kb on - strand, within EX28DRAFT_3001 at 309.770 kb on + strand, within EX28DRAFT_3001 at 309.771 kb on - strand, within EX28DRAFT_3001 at 309.771 kb on - strand, within EX28DRAFT_3001 at 309.771 kb on - strand, within EX28DRAFT_3001 at 309.771 kb on - strand, within EX28DRAFT_3001 at 309.838 kb on + strand, within EX28DRAFT_3001 at 309.838 kb on + strand, within EX28DRAFT_3001 at 309.899 kb on - strand, within EX28DRAFT_3001 at 309.925 kb on + strand, within EX28DRAFT_3001 at 309.925 kb on + strand, within EX28DRAFT_3001 at 309.925 kb on + strand, within EX28DRAFT_3001 at 310.057 kb on + strand, within EX28DRAFT_3001 at 310.058 kb on - strand, within EX28DRAFT_3001
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 307,074 - EX28DRAFT_2998 0.48 +1.1 307,074 - EX28DRAFT_2998 0.48 -0.8 307,074 - EX28DRAFT_2998 0.48 -0.1 307,275 + EX28DRAFT_2998 0.67 -1.0 307,275 + EX28DRAFT_2998 0.67 +0.3 307,275 + EX28DRAFT_2998 0.67 -0.2 307,276 - EX28DRAFT_2998 0.67 -0.7 307,276 - EX28DRAFT_2998 0.67 +1.7 307,276 - EX28DRAFT_2998 0.67 -0.4 307,276 - EX28DRAFT_2998 0.67 -0.1 307,276 - EX28DRAFT_2998 0.67 -0.7 307,346 + EX28DRAFT_2998 0.73 +0.2 307,346 + EX28DRAFT_2998 0.73 +1.4 307,346 + EX28DRAFT_2998 0.73 +0.7 307,347 - EX28DRAFT_2998 0.74 -0.5 307,347 - EX28DRAFT_2998 0.74 +0.1 307,348 + EX28DRAFT_2998 0.74 +0.4 307,540 + -0.4 307,542 + +0.5 307,543 - +0.8 307,547 + -0.1 307,604 + -0.6 307,604 + +0.7 307,605 - -0.2 307,605 - -1.0 307,673 + -0.5 307,743 + EX28DRAFT_2999 0.33 -1.1 307,743 + EX28DRAFT_2999 0.33 -1.5 307,905 + -3.3 307,906 - +1.0 307,908 - +0.2 307,908 - -0.3 307,908 - -1.4 307,924 - +0.2 307,952 - -1.8 308,128 + EX28DRAFT_3000 0.14 +0.1 308,128 + EX28DRAFT_3000 0.14 -0.8 308,129 - EX28DRAFT_3000 0.14 +0.5 308,129 - EX28DRAFT_3000 0.14 -1.6 308,129 - EX28DRAFT_3000 0.14 -0.1 308,129 - EX28DRAFT_3000 0.14 +0.9 308,129 - EX28DRAFT_3000 0.14 +0.2 308,130 + EX28DRAFT_3000 0.14 -0.5 308,130 + EX28DRAFT_3000 0.14 +0.3 308,164 + EX28DRAFT_3000 0.17 -0.5 308,171 + EX28DRAFT_3000 0.18 -0.1 308,171 + EX28DRAFT_3000 0.18 -0.2 308,171 + EX28DRAFT_3000 0.18 -0.1 308,172 - EX28DRAFT_3000 0.18 -0.4 308,176 + EX28DRAFT_3000 0.18 -1.2 308,180 + EX28DRAFT_3000 0.19 +0.3 308,181 - EX28DRAFT_3000 0.19 -1.0 308,181 - EX28DRAFT_3000 0.19 -4.0 308,228 - EX28DRAFT_3000 0.23 +1.3 308,324 - EX28DRAFT_3000 0.32 -0.1 308,333 + EX28DRAFT_3000 0.33 -0.4 308,334 - EX28DRAFT_3000 0.33 +0.2 308,447 + EX28DRAFT_3000 0.43 -0.8 308,494 + EX28DRAFT_3000 0.48 +0.8 308,548 + EX28DRAFT_3000 0.53 -0.4 308,548 + EX28DRAFT_3000 0.53 -0.1 308,549 - EX28DRAFT_3000 0.53 +0.3 308,552 + EX28DRAFT_3000 0.53 -0.6 308,553 - EX28DRAFT_3000 0.53 -0.9 308,585 + EX28DRAFT_3000 0.56 -0.1 308,586 - EX28DRAFT_3000 0.56 -0.5 308,617 + EX28DRAFT_3000 0.59 +0.3 308,686 - EX28DRAFT_3000 0.65 -0.9 308,753 + EX28DRAFT_3000 0.72 +0.3 308,753 + EX28DRAFT_3000 0.72 -0.8 308,754 - EX28DRAFT_3000 0.72 +0.2 308,967 - +0.5 309,012 - +1.0 309,017 + +0.0 309,017 + -0.7 309,017 + -0.1 309,017 + +0.6 309,018 - +0.0 309,044 + -1.0 309,044 + +3.2 309,044 + -0.3 309,194 + +1.7 309,194 + -0.3 309,194 + -0.2 309,194 + +2.0 309,194 + +2.2 309,194 + +0.1 309,195 - +0.0 309,195 - +0.9 309,195 - -0.6 309,195 - +0.1 309,195 - +0.3 309,195 - +0.0 309,195 - -0.0 309,195 - +0.1 309,195 - +2.2 309,195 - -1.1 309,195 - -1.2 309,195 - -0.3 309,225 + -0.1 309,293 - +0.1 309,353 - +0.3 309,365 + -0.6 309,365 + +0.2 309,365 + +0.1 309,366 - +0.1 309,366 - +0.1 309,366 - -0.2 309,367 + +0.4 309,367 + -0.4 309,367 + -2.9 309,368 - +0.2 309,368 - -0.7 309,497 + EX28DRAFT_3001 0.17 +0.7 309,497 + EX28DRAFT_3001 0.17 +0.1 309,498 - EX28DRAFT_3001 0.17 -0.7 309,498 - EX28DRAFT_3001 0.17 +0.8 309,586 + EX28DRAFT_3001 0.24 -0.2 309,586 + EX28DRAFT_3001 0.24 +0.5 309,599 + EX28DRAFT_3001 0.25 -0.7 309,599 + EX28DRAFT_3001 0.25 -0.4 309,599 + EX28DRAFT_3001 0.25 +0.9 309,599 + EX28DRAFT_3001 0.25 -0.3 309,600 - EX28DRAFT_3001 0.25 +0.1 309,600 - EX28DRAFT_3001 0.25 -3.4 309,600 - EX28DRAFT_3001 0.25 +0.0 309,602 - EX28DRAFT_3001 0.25 -0.7 309,602 - EX28DRAFT_3001 0.25 -0.2 309,602 - EX28DRAFT_3001 0.25 -0.5 309,635 + EX28DRAFT_3001 0.28 -0.6 309,635 + EX28DRAFT_3001 0.28 +0.2 309,635 + EX28DRAFT_3001 0.28 -0.1 309,636 - EX28DRAFT_3001 0.28 -1.0 309,691 + EX28DRAFT_3001 0.32 +0.1 309,692 - EX28DRAFT_3001 0.32 -1.6 309,712 + EX28DRAFT_3001 0.34 +0.0 309,713 - EX28DRAFT_3001 0.34 +0.3 309,761 - EX28DRAFT_3001 0.38 +0.1 309,770 + EX28DRAFT_3001 0.39 +0.6 309,771 - EX28DRAFT_3001 0.39 +0.3 309,771 - EX28DRAFT_3001 0.39 -3.5 309,771 - EX28DRAFT_3001 0.39 -0.3 309,771 - EX28DRAFT_3001 0.39 +1.0 309,838 + EX28DRAFT_3001 0.44 -0.4 309,838 + EX28DRAFT_3001 0.44 -0.8 309,899 - EX28DRAFT_3001 0.49 +0.2 309,925 + EX28DRAFT_3001 0.51 -0.2 309,925 + EX28DRAFT_3001 0.51 -1.4 309,925 + EX28DRAFT_3001 0.51 +0.2 310,057 + EX28DRAFT_3001 0.61 +0.7 310,058 - EX28DRAFT_3001 0.62 -0.4
Or see this region's nucleotide sequence