Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2847 and EX28DRAFT_2848 are separated by 9 nucleotides EX28DRAFT_2848 and EX28DRAFT_2849 are separated by 9 nucleotides EX28DRAFT_2849 and EX28DRAFT_2850 are separated by 237 nucleotides
EX28DRAFT_2847: EX28DRAFT_2847 - sulfate adenylyltransferase, large subunit, at 147,145 to 148,569
_2847
EX28DRAFT_2848: EX28DRAFT_2848 - sulfate adenylyltransferase, small subunit, at 148,579 to 149,487
_2848
EX28DRAFT_2849: EX28DRAFT_2849 - uroporphyrin-III C-methyltransferase, at 149,497 to 150,855
_2849
EX28DRAFT_2850: EX28DRAFT_2850 - Predicted aminopeptidases, at 151,093 to 152,136
_2850
Position (kb) 149
150
151 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 148.518 kb on - strand at 148.557 kb on + strand at 148.558 kb on - strand at 148.893 kb on - strand, within EX28DRAFT_2848 at 148.893 kb on - strand, within EX28DRAFT_2848 at 148.976 kb on + strand, within EX28DRAFT_2848 at 149.047 kb on - strand, within EX28DRAFT_2848 at 149.047 kb on - strand, within EX28DRAFT_2848 at 149.047 kb on - strand, within EX28DRAFT_2848 at 149.047 kb on - strand, within EX28DRAFT_2848 at 149.218 kb on - strand, within EX28DRAFT_2848 at 149.241 kb on - strand, within EX28DRAFT_2848 at 149.271 kb on - strand, within EX28DRAFT_2848 at 149.387 kb on + strand, within EX28DRAFT_2848 at 149.387 kb on + strand, within EX28DRAFT_2848 at 149.387 kb on + strand, within EX28DRAFT_2848 at 149.388 kb on - strand, within EX28DRAFT_2848 at 149.388 kb on - strand, within EX28DRAFT_2848 at 149.388 kb on - strand, within EX28DRAFT_2848 at 149.388 kb on - strand, within EX28DRAFT_2848 at 149.388 kb on - strand, within EX28DRAFT_2848 at 149.440 kb on - strand at 149.469 kb on + strand at 149.470 kb on - strand at 149.470 kb on - strand at 149.771 kb on + strand, within EX28DRAFT_2849 at 149.771 kb on + strand, within EX28DRAFT_2849 at 149.772 kb on - strand, within EX28DRAFT_2849 at 149.837 kb on + strand, within EX28DRAFT_2849 at 149.838 kb on - strand, within EX28DRAFT_2849 at 150.685 kb on + strand, within EX28DRAFT_2849 at 150.838 kb on + strand at 150.848 kb on + strand at 150.893 kb on - strand at 150.893 kb on - strand at 150.893 kb on - strand at 150.907 kb on - strand at 150.922 kb on - strand at 150.971 kb on - strand at 150.971 kb on - strand at 151.053 kb on + strand at 151.054 kb on - strand at 151.065 kb on + strand at 151.065 kb on + strand at 151.066 kb on - strand at 151.066 kb on - strand at 151.147 kb on - strand at 151.147 kb on - strand at 151.177 kb on + strand at 151.209 kb on + strand, within EX28DRAFT_2850 at 151.210 kb on - strand, within EX28DRAFT_2850 at 151.340 kb on - strand, within EX28DRAFT_2850 at 151.340 kb on - strand, within EX28DRAFT_2850 at 151.341 kb on + strand, within EX28DRAFT_2850 at 151.342 kb on - strand, within EX28DRAFT_2850 at 151.342 kb on - strand, within EX28DRAFT_2850 at 151.371 kb on + strand, within EX28DRAFT_2850 at 151.372 kb on - strand, within EX28DRAFT_2850 at 151.386 kb on + strand, within EX28DRAFT_2850 at 151.431 kb on + strand, within EX28DRAFT_2850 at 151.431 kb on + strand, within EX28DRAFT_2850 at 151.450 kb on - strand, within EX28DRAFT_2850 at 151.454 kb on - strand, within EX28DRAFT_2850 at 151.729 kb on + strand, within EX28DRAFT_2850 at 151.730 kb on - strand, within EX28DRAFT_2850
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 148,518 - -0.4 148,557 + -0.7 148,558 - +0.3 148,893 - EX28DRAFT_2848 0.35 -0.4 148,893 - EX28DRAFT_2848 0.35 -1.1 148,976 + EX28DRAFT_2848 0.44 +0.2 149,047 - EX28DRAFT_2848 0.51 +0.4 149,047 - EX28DRAFT_2848 0.51 -3.6 149,047 - EX28DRAFT_2848 0.51 -2.1 149,047 - EX28DRAFT_2848 0.51 +0.4 149,218 - EX28DRAFT_2848 0.70 +0.3 149,241 - EX28DRAFT_2848 0.73 -1.6 149,271 - EX28DRAFT_2848 0.76 -2.4 149,387 + EX28DRAFT_2848 0.89 -1.7 149,387 + EX28DRAFT_2848 0.89 -0.3 149,387 + EX28DRAFT_2848 0.89 -1.2 149,388 - EX28DRAFT_2848 0.89 -1.3 149,388 - EX28DRAFT_2848 0.89 -2.7 149,388 - EX28DRAFT_2848 0.89 -0.7 149,388 - EX28DRAFT_2848 0.89 -0.6 149,388 - EX28DRAFT_2848 0.89 -0.9 149,440 - -1.1 149,469 + -0.9 149,470 - -2.6 149,470 - -1.5 149,771 + EX28DRAFT_2849 0.20 -2.2 149,771 + EX28DRAFT_2849 0.20 -1.4 149,772 - EX28DRAFT_2849 0.20 +1.1 149,837 + EX28DRAFT_2849 0.25 -0.6 149,838 - EX28DRAFT_2849 0.25 -2.3 150,685 + EX28DRAFT_2849 0.87 -1.4 150,838 + -1.5 150,848 + -2.6 150,893 - +0.0 150,893 - -1.3 150,893 - +0.8 150,907 - -0.2 150,922 - -0.9 150,971 - +0.1 150,971 - +0.7 151,053 + -3.4 151,054 - +0.7 151,065 + +0.0 151,065 + -0.1 151,066 - -0.4 151,066 - +0.2 151,147 - -0.5 151,147 - +0.4 151,177 + +0.4 151,209 + EX28DRAFT_2850 0.11 +0.4 151,210 - EX28DRAFT_2850 0.11 -0.0 151,340 - EX28DRAFT_2850 0.24 -1.1 151,340 - EX28DRAFT_2850 0.24 +0.3 151,341 + EX28DRAFT_2850 0.24 +0.2 151,342 - EX28DRAFT_2850 0.24 +0.3 151,342 - EX28DRAFT_2850 0.24 +0.2 151,371 + EX28DRAFT_2850 0.27 +0.8 151,372 - EX28DRAFT_2850 0.27 -2.9 151,386 + EX28DRAFT_2850 0.28 +1.0 151,431 + EX28DRAFT_2850 0.32 +1.8 151,431 + EX28DRAFT_2850 0.32 +1.0 151,450 - EX28DRAFT_2850 0.34 -0.8 151,454 - EX28DRAFT_2850 0.35 -0.0 151,729 + EX28DRAFT_2850 0.61 +0.3 151,730 - EX28DRAFT_2850 0.61 -2.5
Or see this region's nucleotide sequence