Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2771 and EX28DRAFT_2772 are separated by 54 nucleotides EX28DRAFT_2772 and EX28DRAFT_2773 are separated by 72 nucleotides EX28DRAFT_2773 and EX28DRAFT_2774 are separated by 8 nucleotides EX28DRAFT_2774 and EX28DRAFT_2775 are separated by 83 nucleotides EX28DRAFT_2775 and EX28DRAFT_2776 overlap by 4 nucleotides
EX28DRAFT_2771: EX28DRAFT_2771 - AraC-type DNA-binding domain-containing proteins, at 80,405 to 81,169
_2771
EX28DRAFT_2772: EX28DRAFT_2772 - S-ribosylhomocysteine lyase (EC 4.4.1.21)/quorum-sensing autoinducer 2 (AI-2) synthesis protein LuxS, at 81,224 to 81,739
_2772
EX28DRAFT_2773: EX28DRAFT_2773 - RNA, at 81,812 to 81,887
_2773
EX28DRAFT_2774: EX28DRAFT_2774 - glutamate-cysteine ligase (EC 6.3.2.2), at 81,896 to 83,440
_2774
EX28DRAFT_2775: EX28DRAFT_2775 - Predicted membrane protein, at 83,524 to 83,952
_2775
EX28DRAFT_2776: EX28DRAFT_2776 - haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED/beta-phosphoglucomutase family hydrolase, at 83,949 to 84,515
_2776
Position (kb)
81
82
83
84 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 81.225 kb on + strand at 81.225 kb on + strand at 81.226 kb on - strand at 81.226 kb on - strand at 81.226 kb on - strand at 81.348 kb on + strand, within EX28DRAFT_2772 at 81.348 kb on + strand, within EX28DRAFT_2772 at 81.349 kb on - strand, within EX28DRAFT_2772 at 81.352 kb on + strand, within EX28DRAFT_2772 at 81.363 kb on + strand, within EX28DRAFT_2772 at 81.477 kb on + strand, within EX28DRAFT_2772 at 81.477 kb on + strand, within EX28DRAFT_2772 at 81.577 kb on + strand, within EX28DRAFT_2772 at 81.578 kb on - strand, within EX28DRAFT_2772 at 81.683 kb on + strand, within EX28DRAFT_2772 at 81.725 kb on - strand at 81.728 kb on + strand at 81.728 kb on + strand at 81.728 kb on + strand at 81.728 kb on + strand at 81.728 kb on + strand at 81.732 kb on - strand at 81.739 kb on + strand at 81.740 kb on - strand at 81.774 kb on + strand at 81.789 kb on + strand at 81.790 kb on - strand at 81.808 kb on + strand at 81.809 kb on - strand at 81.857 kb on - strand, within EX28DRAFT_2773 at 81.857 kb on - strand, within EX28DRAFT_2773 at 81.859 kb on - strand, within EX28DRAFT_2773 at 81.898 kb on - strand at 82.090 kb on + strand, within EX28DRAFT_2774 at 82.298 kb on - strand, within EX28DRAFT_2774 at 82.467 kb on - strand, within EX28DRAFT_2774 at 82.476 kb on + strand, within EX28DRAFT_2774 at 82.677 kb on + strand, within EX28DRAFT_2774 at 82.700 kb on - strand, within EX28DRAFT_2774 at 82.724 kb on - strand, within EX28DRAFT_2774 at 82.777 kb on - strand, within EX28DRAFT_2774 at 82.914 kb on + strand, within EX28DRAFT_2774 at 82.915 kb on - strand, within EX28DRAFT_2774 at 82.989 kb on + strand, within EX28DRAFT_2774 at 83.121 kb on + strand, within EX28DRAFT_2774 at 83.121 kb on + strand, within EX28DRAFT_2774 at 83.147 kb on - strand, within EX28DRAFT_2774 at 83.206 kb on + strand, within EX28DRAFT_2774 at 83.216 kb on - strand, within EX28DRAFT_2774 at 83.279 kb on - strand, within EX28DRAFT_2774 at 83.329 kb on + strand at 83.426 kb on + strand at 83.463 kb on + strand at 83.464 kb on - strand at 83.464 kb on - strand at 83.469 kb on + strand at 83.470 kb on - strand at 83.479 kb on - strand at 83.525 kb on + strand at 83.525 kb on + strand at 83.541 kb on + strand at 83.541 kb on + strand at 83.542 kb on - strand at 83.549 kb on - strand at 83.549 kb on - strand at 83.653 kb on + strand, within EX28DRAFT_2775 at 83.654 kb on - strand, within EX28DRAFT_2775 at 83.665 kb on + strand, within EX28DRAFT_2775 at 83.665 kb on + strand, within EX28DRAFT_2775 at 83.665 kb on + strand, within EX28DRAFT_2775 at 83.666 kb on - strand, within EX28DRAFT_2775 at 83.681 kb on - strand, within EX28DRAFT_2775 at 83.683 kb on + strand, within EX28DRAFT_2775 at 83.776 kb on - strand, within EX28DRAFT_2775 at 83.802 kb on + strand, within EX28DRAFT_2775 at 83.815 kb on + strand, within EX28DRAFT_2775 at 83.818 kb on + strand, within EX28DRAFT_2775 at 83.821 kb on + strand, within EX28DRAFT_2775 at 83.822 kb on - strand, within EX28DRAFT_2775 at 83.822 kb on - strand, within EX28DRAFT_2775 at 83.822 kb on - strand, within EX28DRAFT_2775 at 84.228 kb on + strand, within EX28DRAFT_2776 at 84.229 kb on - strand, within EX28DRAFT_2776 at 84.229 kb on - strand, within EX28DRAFT_2776 at 84.233 kb on + strand, within EX28DRAFT_2776
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 81,225 + -0.5 81,225 + +0.5 81,226 - +0.2 81,226 - +0.4 81,226 - +0.4 81,348 + EX28DRAFT_2772 0.24 +0.9 81,348 + EX28DRAFT_2772 0.24 -0.0 81,349 - EX28DRAFT_2772 0.24 +3.0 81,352 + EX28DRAFT_2772 0.25 +0.3 81,363 + EX28DRAFT_2772 0.27 -0.4 81,477 + EX28DRAFT_2772 0.49 -0.6 81,477 + EX28DRAFT_2772 0.49 +2.1 81,577 + EX28DRAFT_2772 0.68 +0.7 81,578 - EX28DRAFT_2772 0.69 -0.0 81,683 + EX28DRAFT_2772 0.89 +0.4 81,725 - +0.1 81,728 + -0.8 81,728 + +0.9 81,728 + -0.3 81,728 + +0.4 81,728 + +0.5 81,732 - -1.5 81,739 + -0.3 81,740 - +1.0 81,774 + +1.9 81,789 + -2.2 81,790 - +0.1 81,808 + -3.2 81,809 - -0.1 81,857 - EX28DRAFT_2773 0.59 -1.3 81,857 - EX28DRAFT_2773 0.59 +0.2 81,859 - EX28DRAFT_2773 0.62 -0.4 81,898 - -1.2 82,090 + EX28DRAFT_2774 0.13 -2.0 82,298 - EX28DRAFT_2774 0.26 -1.8 82,467 - EX28DRAFT_2774 0.37 -1.8 82,476 + EX28DRAFT_2774 0.38 -5.2 82,677 + EX28DRAFT_2774 0.51 -4.6 82,700 - EX28DRAFT_2774 0.52 -3.2 82,724 - EX28DRAFT_2774 0.54 -4.7 82,777 - EX28DRAFT_2774 0.57 -4.1 82,914 + EX28DRAFT_2774 0.66 -3.6 82,915 - EX28DRAFT_2774 0.66 -0.9 82,989 + EX28DRAFT_2774 0.71 -4.3 83,121 + EX28DRAFT_2774 0.79 -2.9 83,121 + EX28DRAFT_2774 0.79 -1.2 83,147 - EX28DRAFT_2774 0.81 -4.3 83,206 + EX28DRAFT_2774 0.85 -3.4 83,216 - EX28DRAFT_2774 0.85 -0.2 83,279 - EX28DRAFT_2774 0.90 +0.1 83,329 + -3.0 83,426 + -2.2 83,463 + -2.7 83,464 - -2.5 83,464 - -1.5 83,469 + -1.9 83,470 - -1.0 83,479 - -1.0 83,525 + -3.1 83,525 + -5.1 83,541 + -2.0 83,541 + -2.3 83,542 - -0.6 83,549 - -0.2 83,549 - -1.2 83,653 + EX28DRAFT_2775 0.30 -2.0 83,654 - EX28DRAFT_2775 0.30 -1.2 83,665 + EX28DRAFT_2775 0.33 -1.7 83,665 + EX28DRAFT_2775 0.33 -1.1 83,665 + EX28DRAFT_2775 0.33 -4.3 83,666 - EX28DRAFT_2775 0.33 +0.2 83,681 - EX28DRAFT_2775 0.37 +0.8 83,683 + EX28DRAFT_2775 0.37 -1.8 83,776 - EX28DRAFT_2775 0.59 -0.5 83,802 + EX28DRAFT_2775 0.65 -1.7 83,815 + EX28DRAFT_2775 0.68 +0.5 83,818 + EX28DRAFT_2775 0.69 +0.4 83,821 + EX28DRAFT_2775 0.69 +0.6 83,822 - EX28DRAFT_2775 0.69 -0.4 83,822 - EX28DRAFT_2775 0.69 -0.5 83,822 - EX28DRAFT_2775 0.69 -0.2 84,228 + EX28DRAFT_2776 0.49 -0.4 84,229 - EX28DRAFT_2776 0.49 +0.2 84,229 - EX28DRAFT_2776 0.49 -0.1 84,233 + EX28DRAFT_2776 0.50 -0.3
Or see this region's nucleotide sequence