Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2107 and EX28DRAFT_2108 are separated by 48 nucleotides EX28DRAFT_2108 and EX28DRAFT_2109 are separated by 19 nucleotides EX28DRAFT_2109 and EX28DRAFT_2110 are separated by 171 nucleotides EX28DRAFT_2110 and EX28DRAFT_2111 overlap by 8 nucleotides
EX28DRAFT_2107: EX28DRAFT_2107 - hypothetical protein, at 2,129,790 to 2,130,389
_2107
EX28DRAFT_2108: EX28DRAFT_2108 - phage portal protein, PBSX family, at 2,130,438 to 2,131,037
_2108
EX28DRAFT_2109: EX28DRAFT_2109 - Reverse transcriptase (RNA-dependent DNA polymerase), at 2,131,057 to 2,132,040
_2109
EX28DRAFT_2110: EX28DRAFT_2110 - hypothetical protein, at 2,132,212 to 2,132,949
_2110
EX28DRAFT_2111: EX28DRAFT_2111 - hypothetical protein, at 2,132,942 to 2,133,487
_2111
Position (kb)
2131
2132
2133 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2130.076 kb on + strand, within EX28DRAFT_2107 at 2130.088 kb on + strand, within EX28DRAFT_2107 at 2130.088 kb on + strand, within EX28DRAFT_2107 at 2130.089 kb on - strand, within EX28DRAFT_2107 at 2130.138 kb on - strand, within EX28DRAFT_2107 at 2130.138 kb on - strand, within EX28DRAFT_2107 at 2130.376 kb on + strand at 2130.376 kb on + strand at 2130.420 kb on + strand at 2130.425 kb on + strand at 2130.425 kb on + strand at 2130.426 kb on - strand at 2130.471 kb on - strand at 2130.483 kb on + strand at 2130.484 kb on - strand at 2130.558 kb on + strand, within EX28DRAFT_2108 at 2130.559 kb on - strand, within EX28DRAFT_2108 at 2130.618 kb on + strand, within EX28DRAFT_2108 at 2130.618 kb on + strand, within EX28DRAFT_2108 at 2130.622 kb on - strand, within EX28DRAFT_2108 at 2130.622 kb on - strand, within EX28DRAFT_2108 at 2130.648 kb on - strand, within EX28DRAFT_2108 at 2130.648 kb on - strand, within EX28DRAFT_2108 at 2130.654 kb on + strand, within EX28DRAFT_2108 at 2130.654 kb on + strand, within EX28DRAFT_2108 at 2130.655 kb on - strand, within EX28DRAFT_2108 at 2130.655 kb on - strand, within EX28DRAFT_2108 at 2130.655 kb on - strand, within EX28DRAFT_2108 at 2130.655 kb on - strand, within EX28DRAFT_2108 at 2130.655 kb on - strand, within EX28DRAFT_2108 at 2130.655 kb on - strand, within EX28DRAFT_2108 at 2130.656 kb on + strand, within EX28DRAFT_2108 at 2130.657 kb on - strand, within EX28DRAFT_2108 at 2130.780 kb on - strand, within EX28DRAFT_2108 at 2130.815 kb on + strand, within EX28DRAFT_2108 at 2130.819 kb on + strand, within EX28DRAFT_2108 at 2130.844 kb on - strand, within EX28DRAFT_2108 at 2130.894 kb on + strand, within EX28DRAFT_2108 at 2131.010 kb on - strand at 2131.025 kb on + strand at 2131.082 kb on - strand at 2131.254 kb on + strand, within EX28DRAFT_2109 at 2131.258 kb on + strand, within EX28DRAFT_2109 at 2131.258 kb on + strand, within EX28DRAFT_2109 at 2131.506 kb on + strand, within EX28DRAFT_2109 at 2131.576 kb on - strand, within EX28DRAFT_2109 at 2132.054 kb on + strand at 2132.208 kb on - strand at 2132.281 kb on + strand at 2132.281 kb on + strand at 2132.313 kb on - strand, within EX28DRAFT_2110 at 2132.424 kb on + strand, within EX28DRAFT_2110 at 2132.695 kb on + strand, within EX28DRAFT_2110 at 2132.995 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 2,130,076 + EX28DRAFT_2107 0.48 +0.6 2,130,088 + EX28DRAFT_2107 0.50 -1.4 2,130,088 + EX28DRAFT_2107 0.50 -0.5 2,130,089 - EX28DRAFT_2107 0.50 +0.3 2,130,138 - EX28DRAFT_2107 0.58 -1.0 2,130,138 - EX28DRAFT_2107 0.58 +0.1 2,130,376 + +0.2 2,130,376 + +0.4 2,130,420 + -1.3 2,130,425 + +0.0 2,130,425 + +0.1 2,130,426 - -0.3 2,130,471 - -0.1 2,130,483 + -0.2 2,130,484 - +0.6 2,130,558 + EX28DRAFT_2108 0.20 +0.4 2,130,559 - EX28DRAFT_2108 0.20 +1.4 2,130,618 + EX28DRAFT_2108 0.30 -0.6 2,130,618 + EX28DRAFT_2108 0.30 -1.3 2,130,622 - EX28DRAFT_2108 0.31 +0.6 2,130,622 - EX28DRAFT_2108 0.31 -3.2 2,130,648 - EX28DRAFT_2108 0.35 -0.2 2,130,648 - EX28DRAFT_2108 0.35 -0.1 2,130,654 + EX28DRAFT_2108 0.36 +0.9 2,130,654 + EX28DRAFT_2108 0.36 -0.5 2,130,655 - EX28DRAFT_2108 0.36 -1.3 2,130,655 - EX28DRAFT_2108 0.36 +1.0 2,130,655 - EX28DRAFT_2108 0.36 -0.1 2,130,655 - EX28DRAFT_2108 0.36 -1.3 2,130,655 - EX28DRAFT_2108 0.36 -0.4 2,130,655 - EX28DRAFT_2108 0.36 +0.7 2,130,656 + EX28DRAFT_2108 0.36 -0.0 2,130,657 - EX28DRAFT_2108 0.36 -0.7 2,130,780 - EX28DRAFT_2108 0.57 +1.0 2,130,815 + EX28DRAFT_2108 0.63 +0.1 2,130,819 + EX28DRAFT_2108 0.64 -0.4 2,130,844 - EX28DRAFT_2108 0.68 +0.1 2,130,894 + EX28DRAFT_2108 0.76 +0.7 2,131,010 - -1.2 2,131,025 + -0.6 2,131,082 - -0.5 2,131,254 + EX28DRAFT_2109 0.20 -0.3 2,131,258 + EX28DRAFT_2109 0.20 +0.5 2,131,258 + EX28DRAFT_2109 0.20 -1.9 2,131,506 + EX28DRAFT_2109 0.46 -0.3 2,131,576 - EX28DRAFT_2109 0.53 -0.6 2,132,054 + -0.4 2,132,208 - -1.3 2,132,281 + +0.2 2,132,281 + -0.6 2,132,313 - EX28DRAFT_2110 0.14 -0.2 2,132,424 + EX28DRAFT_2110 0.29 +1.8 2,132,695 + EX28DRAFT_2110 0.65 -0.5 2,132,995 + -2.3
Or see this region's nucleotide sequence