Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1940 and EX28DRAFT_1941 are separated by 10 nucleotides EX28DRAFT_1941 and EX28DRAFT_1942 overlap by 4 nucleotides EX28DRAFT_1942 and EX28DRAFT_1943 overlap by 4 nucleotides EX28DRAFT_1943 and EX28DRAFT_1944 are separated by 69 nucleotides
EX28DRAFT_1940: EX28DRAFT_1940 - Gamma-glutamyltransferase, at 1,945,766 to 1,947,349
_1940
EX28DRAFT_1941: EX28DRAFT_1941 - Protein of unknown function (DUF4089), at 1,947,360 to 1,947,548
_1941
EX28DRAFT_1942: EX28DRAFT_1942 - amidohydrolase, AtzE family, at 1,947,545 to 1,948,942
_1942
EX28DRAFT_1943: EX28DRAFT_1943 - Protein of unknown function (DUF3225), at 1,948,939 to 1,949,325
_1943
EX28DRAFT_1944: EX28DRAFT_1944 - putative urate catabolism protein, at 1,949,395 to 1,950,327
_1944
Position (kb) 1947
1948
1949 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1946.551 kb on - strand, within EX28DRAFT_1940 at 1946.576 kb on - strand, within EX28DRAFT_1940 at 1946.842 kb on + strand, within EX28DRAFT_1940 at 1946.843 kb on - strand, within EX28DRAFT_1940 at 1946.846 kb on + strand, within EX28DRAFT_1940 at 1947.099 kb on + strand, within EX28DRAFT_1940 at 1947.099 kb on + strand, within EX28DRAFT_1940 at 1947.234 kb on + strand at 1947.234 kb on + strand at 1947.234 kb on + strand at 1947.473 kb on - strand, within EX28DRAFT_1941 at 1947.535 kb on + strand at 1947.536 kb on - strand at 1947.537 kb on + strand at 1947.537 kb on + strand at 1947.537 kb on + strand at 1947.538 kb on - strand at 1947.538 kb on - strand at 1947.565 kb on + strand at 1947.916 kb on + strand, within EX28DRAFT_1942 at 1947.916 kb on + strand, within EX28DRAFT_1942 at 1947.917 kb on - strand, within EX28DRAFT_1942 at 1948.465 kb on + strand, within EX28DRAFT_1942 at 1948.587 kb on - strand, within EX28DRAFT_1942 at 1948.587 kb on - strand, within EX28DRAFT_1942 at 1948.780 kb on - strand, within EX28DRAFT_1942 at 1949.000 kb on - strand, within EX28DRAFT_1943 at 1949.396 kb on + strand at 1949.396 kb on + strand at 1949.397 kb on - strand at 1949.397 kb on - strand at 1949.478 kb on + strand at 1949.479 kb on - strand at 1949.479 kb on - strand at 1949.479 kb on - strand at 1949.480 kb on + strand at 1949.609 kb on + strand, within EX28DRAFT_1944 at 1949.610 kb on - strand, within EX28DRAFT_1944 at 1949.613 kb on + strand, within EX28DRAFT_1944 at 1949.613 kb on + strand, within EX28DRAFT_1944 at 1949.613 kb on + strand, within EX28DRAFT_1944 at 1949.614 kb on - strand, within EX28DRAFT_1944 at 1949.615 kb on + strand, within EX28DRAFT_1944 at 1949.615 kb on + strand, within EX28DRAFT_1944 at 1949.615 kb on + strand, within EX28DRAFT_1944 at 1949.615 kb on + strand, within EX28DRAFT_1944 at 1949.616 kb on - strand, within EX28DRAFT_1944 at 1949.616 kb on - strand, within EX28DRAFT_1944 at 1949.616 kb on - strand, within EX28DRAFT_1944 at 1949.759 kb on + strand, within EX28DRAFT_1944 at 1949.832 kb on - strand, within EX28DRAFT_1944 at 1949.925 kb on - strand, within EX28DRAFT_1944
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 1,946,551 - EX28DRAFT_1940 0.50 -1.4 1,946,576 - EX28DRAFT_1940 0.51 +0.4 1,946,842 + EX28DRAFT_1940 0.68 +0.5 1,946,843 - EX28DRAFT_1940 0.68 +0.7 1,946,846 + EX28DRAFT_1940 0.68 +0.2 1,947,099 + EX28DRAFT_1940 0.84 +0.1 1,947,099 + EX28DRAFT_1940 0.84 -0.6 1,947,234 + +0.5 1,947,234 + -0.1 1,947,234 + -0.0 1,947,473 - EX28DRAFT_1941 0.60 -0.5 1,947,535 + -0.3 1,947,536 - +1.6 1,947,537 + +1.2 1,947,537 + -0.7 1,947,537 + +0.1 1,947,538 - -0.0 1,947,538 - -1.2 1,947,565 + +0.1 1,947,916 + EX28DRAFT_1942 0.27 -1.9 1,947,916 + EX28DRAFT_1942 0.27 -3.4 1,947,917 - EX28DRAFT_1942 0.27 -1.1 1,948,465 + EX28DRAFT_1942 0.66 -0.3 1,948,587 - EX28DRAFT_1942 0.75 +1.5 1,948,587 - EX28DRAFT_1942 0.75 -1.0 1,948,780 - EX28DRAFT_1942 0.88 -1.3 1,949,000 - EX28DRAFT_1943 0.16 +0.0 1,949,396 + +0.9 1,949,396 + +0.0 1,949,397 - +2.9 1,949,397 - -0.4 1,949,478 + +0.5 1,949,479 - +0.3 1,949,479 - +0.1 1,949,479 - -0.5 1,949,480 + -0.1 1,949,609 + EX28DRAFT_1944 0.23 +0.1 1,949,610 - EX28DRAFT_1944 0.23 +0.4 1,949,613 + EX28DRAFT_1944 0.23 -0.2 1,949,613 + EX28DRAFT_1944 0.23 -0.8 1,949,613 + EX28DRAFT_1944 0.23 -1.2 1,949,614 - EX28DRAFT_1944 0.23 +0.5 1,949,615 + EX28DRAFT_1944 0.24 -0.8 1,949,615 + EX28DRAFT_1944 0.24 +0.2 1,949,615 + EX28DRAFT_1944 0.24 +0.5 1,949,615 + EX28DRAFT_1944 0.24 +0.1 1,949,616 - EX28DRAFT_1944 0.24 -0.1 1,949,616 - EX28DRAFT_1944 0.24 +0.9 1,949,616 - EX28DRAFT_1944 0.24 +0.4 1,949,759 + EX28DRAFT_1944 0.39 -3.0 1,949,832 - EX28DRAFT_1944 0.47 +0.0 1,949,925 - EX28DRAFT_1944 0.57 +0.2
Or see this region's nucleotide sequence