Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1546 and EX28DRAFT_1547 are separated by 39 nucleotides EX28DRAFT_1547 and EX28DRAFT_1548 overlap by 4 nucleotides EX28DRAFT_1548 and EX28DRAFT_1549 are separated by 92 nucleotides
EX28DRAFT_1546: EX28DRAFT_1546 - transporter, basic amino acid/polyamine antiporter (APA) family, at 1,562,222 to 1,563,604
_1546
EX28DRAFT_1547: EX28DRAFT_1547 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 1,563,644 to 1,564,366
_1547
EX28DRAFT_1548: EX28DRAFT_1548 - Uncharacterized membrane protein required for alginate biosynthesis, at 1,564,363 to 1,564,698
_1548
EX28DRAFT_1549: EX28DRAFT_1549 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 1,564,791 to 1,565,543
_1549
Position (kb)
1563
1564
1565 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1562.658 kb on + strand, within EX28DRAFT_1546 at 1562.658 kb on + strand, within EX28DRAFT_1546 at 1562.731 kb on + strand, within EX28DRAFT_1546 at 1562.746 kb on + strand, within EX28DRAFT_1546 at 1563.284 kb on + strand, within EX28DRAFT_1546 at 1563.284 kb on + strand, within EX28DRAFT_1546 at 1563.285 kb on - strand, within EX28DRAFT_1546 at 1563.329 kb on + strand, within EX28DRAFT_1546 at 1563.330 kb on - strand, within EX28DRAFT_1546 at 1563.336 kb on + strand, within EX28DRAFT_1546 at 1563.347 kb on + strand, within EX28DRAFT_1546 at 1563.347 kb on + strand, within EX28DRAFT_1546 at 1563.348 kb on - strand, within EX28DRAFT_1546 at 1563.381 kb on - strand, within EX28DRAFT_1546 at 1563.381 kb on - strand, within EX28DRAFT_1546 at 1563.404 kb on - strand, within EX28DRAFT_1546 at 1563.425 kb on + strand, within EX28DRAFT_1546 at 1563.440 kb on - strand, within EX28DRAFT_1546 at 1563.490 kb on + strand at 1563.495 kb on - strand at 1563.549 kb on + strand at 1563.550 kb on - strand at 1563.562 kb on - strand at 1563.640 kb on + strand at 1563.752 kb on + strand, within EX28DRAFT_1547 at 1563.764 kb on + strand, within EX28DRAFT_1547 at 1564.046 kb on - strand, within EX28DRAFT_1547 at 1564.298 kb on + strand at 1564.299 kb on - strand at 1564.316 kb on + strand at 1564.316 kb on + strand at 1564.391 kb on - strand at 1564.484 kb on + strand, within EX28DRAFT_1548 at 1564.503 kb on + strand, within EX28DRAFT_1548 at 1564.530 kb on + strand, within EX28DRAFT_1548 at 1564.566 kb on + strand, within EX28DRAFT_1548 at 1564.567 kb on - strand, within EX28DRAFT_1548 at 1564.567 kb on - strand, within EX28DRAFT_1548 at 1564.567 kb on - strand, within EX28DRAFT_1548 at 1564.567 kb on - strand, within EX28DRAFT_1548 at 1564.568 kb on + strand, within EX28DRAFT_1548 at 1564.568 kb on + strand, within EX28DRAFT_1548 at 1564.569 kb on - strand, within EX28DRAFT_1548 at 1564.618 kb on - strand, within EX28DRAFT_1548 at 1564.619 kb on + strand, within EX28DRAFT_1548 at 1564.619 kb on + strand, within EX28DRAFT_1548 at 1564.655 kb on - strand, within EX28DRAFT_1548 at 1564.655 kb on - strand, within EX28DRAFT_1548 at 1564.683 kb on + strand at 1564.728 kb on - strand at 1564.794 kb on + strand at 1564.794 kb on + strand at 1564.795 kb on - strand at 1564.795 kb on - strand at 1564.795 kb on - strand at 1564.795 kb on - strand at 1564.795 kb on - strand at 1564.826 kb on + strand at 1564.827 kb on - strand at 1564.842 kb on + strand at 1564.843 kb on - strand at 1564.901 kb on + strand, within EX28DRAFT_1549 at 1565.048 kb on + strand, within EX28DRAFT_1549 at 1565.118 kb on + strand, within EX28DRAFT_1549 at 1565.119 kb on - strand, within EX28DRAFT_1549
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 1,562,658 + EX28DRAFT_1546 0.32 +0.8 1,562,658 + EX28DRAFT_1546 0.32 -3.7 1,562,731 + EX28DRAFT_1546 0.37 -0.9 1,562,746 + EX28DRAFT_1546 0.38 -0.7 1,563,284 + EX28DRAFT_1546 0.77 +0.1 1,563,284 + EX28DRAFT_1546 0.77 -1.9 1,563,285 - EX28DRAFT_1546 0.77 -0.0 1,563,329 + EX28DRAFT_1546 0.80 +2.0 1,563,330 - EX28DRAFT_1546 0.80 -0.0 1,563,336 + EX28DRAFT_1546 0.81 -1.1 1,563,347 + EX28DRAFT_1546 0.81 +0.6 1,563,347 + EX28DRAFT_1546 0.81 +0.4 1,563,348 - EX28DRAFT_1546 0.81 +0.4 1,563,381 - EX28DRAFT_1546 0.84 -0.1 1,563,381 - EX28DRAFT_1546 0.84 +0.4 1,563,404 - EX28DRAFT_1546 0.85 +0.2 1,563,425 + EX28DRAFT_1546 0.87 +0.1 1,563,440 - EX28DRAFT_1546 0.88 +0.4 1,563,490 + +0.5 1,563,495 - +1.9 1,563,549 + -0.4 1,563,550 - -0.4 1,563,562 - +2.2 1,563,640 + -1.9 1,563,752 + EX28DRAFT_1547 0.15 -1.7 1,563,764 + EX28DRAFT_1547 0.17 -2.0 1,564,046 - EX28DRAFT_1547 0.56 -0.3 1,564,298 + +0.2 1,564,299 - -0.8 1,564,316 + -0.2 1,564,316 + -0.4 1,564,391 - +0.7 1,564,484 + EX28DRAFT_1548 0.36 -1.8 1,564,503 + EX28DRAFT_1548 0.42 -0.5 1,564,530 + EX28DRAFT_1548 0.50 -1.6 1,564,566 + EX28DRAFT_1548 0.60 +1.4 1,564,567 - EX28DRAFT_1548 0.61 -0.8 1,564,567 - EX28DRAFT_1548 0.61 -2.0 1,564,567 - EX28DRAFT_1548 0.61 +0.7 1,564,567 - EX28DRAFT_1548 0.61 +0.6 1,564,568 + EX28DRAFT_1548 0.61 -0.3 1,564,568 + EX28DRAFT_1548 0.61 -0.2 1,564,569 - EX28DRAFT_1548 0.61 -0.3 1,564,618 - EX28DRAFT_1548 0.76 -0.7 1,564,619 + EX28DRAFT_1548 0.76 +0.0 1,564,619 + EX28DRAFT_1548 0.76 -0.6 1,564,655 - EX28DRAFT_1548 0.87 -1.3 1,564,655 - EX28DRAFT_1548 0.87 +0.3 1,564,683 + -0.6 1,564,728 - -0.5 1,564,794 + -0.9 1,564,794 + -0.3 1,564,795 - +0.3 1,564,795 - -1.7 1,564,795 - -1.8 1,564,795 - +0.6 1,564,795 - -0.5 1,564,826 + -0.1 1,564,827 - -0.0 1,564,842 + -1.8 1,564,843 - -0.6 1,564,901 + EX28DRAFT_1549 0.15 +0.5 1,565,048 + EX28DRAFT_1549 0.34 +0.0 1,565,118 + EX28DRAFT_1549 0.43 +0.0 1,565,119 - EX28DRAFT_1549 0.44 -0.3
Or see this region's nucleotide sequence