Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1538 and EX28DRAFT_1539 are separated by 10 nucleotides EX28DRAFT_1539 and EX28DRAFT_1540 are separated by 183 nucleotides EX28DRAFT_1540 and EX28DRAFT_1541 are separated by 134 nucleotides EX28DRAFT_1541 and EX28DRAFT_1542 are separated by 109 nucleotides
EX28DRAFT_1538: EX28DRAFT_1538 - p-Aminobenzoyl-glutamate transporter family, at 1,553,130 to 1,554,656
_1538
EX28DRAFT_1539: EX28DRAFT_1539 - O-6-methylguanine DNA methyltransferase, at 1,554,667 to 1,555,182
_1539
EX28DRAFT_1540: EX28DRAFT_1540 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 1,555,366 to 1,556,118
_1540
EX28DRAFT_1541: EX28DRAFT_1541 - hypothetical protein, at 1,556,253 to 1,556,447
_1541
EX28DRAFT_1542: EX28DRAFT_1542 - Universal stress protein UspA and related nucleotide-binding proteins, at 1,556,557 to 1,557,507
_1542
Position (kb)
1555
1556
1557 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1554.390 kb on - strand, within EX28DRAFT_1538 at 1554.493 kb on + strand, within EX28DRAFT_1538 at 1554.537 kb on + strand at 1554.538 kb on - strand at 1554.538 kb on - strand at 1554.540 kb on + strand at 1554.580 kb on + strand at 1554.606 kb on + strand at 1554.607 kb on - strand at 1554.638 kb on - strand at 1554.640 kb on - strand at 1554.654 kb on + strand at 1554.825 kb on + strand, within EX28DRAFT_1539 at 1554.826 kb on - strand, within EX28DRAFT_1539 at 1554.834 kb on + strand, within EX28DRAFT_1539 at 1554.944 kb on + strand, within EX28DRAFT_1539 at 1555.011 kb on - strand, within EX28DRAFT_1539 at 1555.120 kb on + strand, within EX28DRAFT_1539 at 1555.121 kb on - strand, within EX28DRAFT_1539 at 1555.203 kb on - strand at 1555.306 kb on + strand at 1555.310 kb on + strand at 1555.311 kb on - strand at 1555.311 kb on - strand at 1555.348 kb on + strand at 1555.386 kb on + strand at 1555.386 kb on + strand at 1555.417 kb on - strand at 1555.542 kb on + strand, within EX28DRAFT_1540 at 1555.600 kb on + strand, within EX28DRAFT_1540 at 1555.600 kb on + strand, within EX28DRAFT_1540 at 1555.884 kb on + strand, within EX28DRAFT_1540 at 1555.885 kb on - strand, within EX28DRAFT_1540 at 1555.972 kb on + strand, within EX28DRAFT_1540 at 1555.973 kb on - strand, within EX28DRAFT_1540 at 1556.050 kb on - strand at 1556.054 kb on - strand at 1556.174 kb on + strand at 1556.208 kb on + strand at 1556.208 kb on + strand at 1556.209 kb on - strand at 1556.303 kb on + strand, within EX28DRAFT_1541 at 1556.303 kb on + strand, within EX28DRAFT_1541 at 1556.356 kb on - strand, within EX28DRAFT_1541 at 1556.406 kb on - strand, within EX28DRAFT_1541 at 1556.434 kb on + strand at 1556.503 kb on - strand at 1556.503 kb on - strand at 1556.505 kb on - strand at 1556.505 kb on - strand at 1556.506 kb on + strand at 1556.506 kb on + strand at 1556.507 kb on - strand at 1556.507 kb on - strand at 1556.524 kb on - strand at 1556.541 kb on + strand at 1556.542 kb on - strand at 1556.582 kb on + strand at 1556.586 kb on + strand at 1556.633 kb on - strand at 1556.633 kb on - strand at 1556.692 kb on + strand, within EX28DRAFT_1542 at 1556.788 kb on + strand, within EX28DRAFT_1542 at 1556.788 kb on + strand, within EX28DRAFT_1542 at 1556.791 kb on + strand, within EX28DRAFT_1542 at 1556.792 kb on - strand, within EX28DRAFT_1542 at 1556.818 kb on - strand, within EX28DRAFT_1542 at 1556.820 kb on + strand, within EX28DRAFT_1542 at 1556.820 kb on + strand, within EX28DRAFT_1542 at 1556.964 kb on + strand, within EX28DRAFT_1542
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 1,554,390 - EX28DRAFT_1538 0.83 -0.5 1,554,493 + EX28DRAFT_1538 0.89 +0.1 1,554,537 + +0.1 1,554,538 - +0.6 1,554,538 - -1.6 1,554,540 + -0.6 1,554,580 + -0.2 1,554,606 + +0.2 1,554,607 - -0.6 1,554,638 - -1.3 1,554,640 - +0.5 1,554,654 + -0.3 1,554,825 + EX28DRAFT_1539 0.31 -2.8 1,554,826 - EX28DRAFT_1539 0.31 -0.1 1,554,834 + EX28DRAFT_1539 0.32 +0.2 1,554,944 + EX28DRAFT_1539 0.54 -0.2 1,555,011 - EX28DRAFT_1539 0.67 -0.6 1,555,120 + EX28DRAFT_1539 0.88 -1.5 1,555,121 - EX28DRAFT_1539 0.88 -0.4 1,555,203 - +0.1 1,555,306 + +0.0 1,555,310 + -0.7 1,555,311 - +0.5 1,555,311 - -0.0 1,555,348 + -0.3 1,555,386 + +0.3 1,555,386 + +0.0 1,555,417 - -0.5 1,555,542 + EX28DRAFT_1540 0.23 -0.6 1,555,600 + EX28DRAFT_1540 0.31 -0.0 1,555,600 + EX28DRAFT_1540 0.31 +0.3 1,555,884 + EX28DRAFT_1540 0.69 +0.5 1,555,885 - EX28DRAFT_1540 0.69 -0.1 1,555,972 + EX28DRAFT_1540 0.80 +0.4 1,555,973 - EX28DRAFT_1540 0.81 -1.2 1,556,050 - +0.0 1,556,054 - -0.1 1,556,174 + +0.2 1,556,208 + -3.0 1,556,208 + +1.6 1,556,209 - +0.2 1,556,303 + EX28DRAFT_1541 0.26 +0.5 1,556,303 + EX28DRAFT_1541 0.26 +0.9 1,556,356 - EX28DRAFT_1541 0.53 -0.0 1,556,406 - EX28DRAFT_1541 0.78 -1.1 1,556,434 + -1.1 1,556,503 - +0.4 1,556,503 - -0.2 1,556,505 - -0.1 1,556,505 - -1.8 1,556,506 + -0.9 1,556,506 + +2.3 1,556,507 - -1.7 1,556,507 - +0.1 1,556,524 - -2.2 1,556,541 + +0.4 1,556,542 - +0.4 1,556,582 + -1.6 1,556,586 + -1.1 1,556,633 - -0.9 1,556,633 - -1.6 1,556,692 + EX28DRAFT_1542 0.14 -3.7 1,556,788 + EX28DRAFT_1542 0.24 -0.8 1,556,788 + EX28DRAFT_1542 0.24 -0.7 1,556,791 + EX28DRAFT_1542 0.25 -1.5 1,556,792 - EX28DRAFT_1542 0.25 -4.2 1,556,818 - EX28DRAFT_1542 0.27 -1.8 1,556,820 + EX28DRAFT_1542 0.28 -0.8 1,556,820 + EX28DRAFT_1542 0.28 -1.7 1,556,964 + EX28DRAFT_1542 0.43 -0.6
Or see this region's nucleotide sequence