Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0532 and EX28DRAFT_0533 are separated by 40 nucleotides EX28DRAFT_0533 and EX28DRAFT_0534 overlap by 7 nucleotides EX28DRAFT_0534 and EX28DRAFT_0535 overlap by 19 nucleotides EX28DRAFT_0535 and EX28DRAFT_0536 overlap by 1 nucleotides
EX28DRAFT_0532: EX28DRAFT_0532 - Chain length determinant protein, at 527,068 to 528,048
_0532
EX28DRAFT_0533: EX28DRAFT_0533 - phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)/phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31), at 528,089 to 528,700
_0533
EX28DRAFT_0534: EX28DRAFT_0534 - imidazoleglycerol phosphate synthase, cyclase subunit, at 528,694 to 529,470
_0534
EX28DRAFT_0535: EX28DRAFT_0535 - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC 5.3.1.16), at 529,452 to 530,189
_0535
EX28DRAFT_0536: EX28DRAFT_0536 - imidazole glycerol phosphate synthase, glutamine amidotransferase subunit, at 530,189 to 530,779
_0536
Position (kb) 528
529
530 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 527.735 kb on + strand, within EX28DRAFT_0532 at 527.735 kb on + strand, within EX28DRAFT_0532 at 527.830 kb on + strand, within EX28DRAFT_0532 at 527.874 kb on - strand, within EX28DRAFT_0532 at 527.874 kb on - strand, within EX28DRAFT_0532 at 527.886 kb on + strand, within EX28DRAFT_0532 at 527.887 kb on - strand, within EX28DRAFT_0532 at 527.896 kb on + strand, within EX28DRAFT_0532 at 527.999 kb on + strand at 528.023 kb on + strand at 528.031 kb on + strand at 528.075 kb on + strand at 528.168 kb on + strand, within EX28DRAFT_0533 at 528.168 kb on + strand, within EX28DRAFT_0533 at 528.212 kb on + strand, within EX28DRAFT_0533 at 528.213 kb on - strand, within EX28DRAFT_0533 at 528.213 kb on - strand, within EX28DRAFT_0533 at 528.265 kb on + strand, within EX28DRAFT_0533 at 528.266 kb on - strand, within EX28DRAFT_0533 at 528.301 kb on - strand, within EX28DRAFT_0533 at 528.301 kb on - strand, within EX28DRAFT_0533 at 528.592 kb on + strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.595 kb on - strand, within EX28DRAFT_0533 at 528.731 kb on + strand at 528.750 kb on + strand at 528.751 kb on - strand at 528.751 kb on - strand at 529.053 kb on - strand, within EX28DRAFT_0534 at 529.074 kb on - strand, within EX28DRAFT_0534 at 529.098 kb on + strand, within EX28DRAFT_0534 at 529.099 kb on - strand, within EX28DRAFT_0534 at 529.177 kb on - strand, within EX28DRAFT_0534 at 529.322 kb on + strand, within EX28DRAFT_0534 at 529.323 kb on - strand, within EX28DRAFT_0534 at 529.435 kb on + strand at 529.450 kb on + strand at 529.450 kb on + strand at 529.450 kb on + strand at 529.451 kb on - strand at 529.451 kb on - strand at 529.490 kb on + strand at 529.566 kb on + strand, within EX28DRAFT_0535 at 529.566 kb on + strand, within EX28DRAFT_0535 at 529.566 kb on + strand, within EX28DRAFT_0535 at 529.567 kb on - strand, within EX28DRAFT_0535 at 530.055 kb on + strand, within EX28DRAFT_0535 at 530.055 kb on + strand, within EX28DRAFT_0535 at 530.157 kb on - strand at 530.184 kb on - strand at 530.305 kb on - strand, within EX28DRAFT_0536 at 530.346 kb on + strand, within EX28DRAFT_0536
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 527,735 + EX28DRAFT_0532 0.68 +0.9 527,735 + EX28DRAFT_0532 0.68 -0.6 527,830 + EX28DRAFT_0532 0.78 +0.9 527,874 - EX28DRAFT_0532 0.82 -0.1 527,874 - EX28DRAFT_0532 0.82 -0.6 527,886 + EX28DRAFT_0532 0.83 -1.9 527,887 - EX28DRAFT_0532 0.83 +0.4 527,896 + EX28DRAFT_0532 0.84 +0.5 527,999 + -0.1 528,023 + -0.9 528,031 + -0.1 528,075 + +0.2 528,168 + EX28DRAFT_0533 0.13 +0.5 528,168 + EX28DRAFT_0533 0.13 -2.3 528,212 + EX28DRAFT_0533 0.20 -0.6 528,213 - EX28DRAFT_0533 0.20 -3.0 528,213 - EX28DRAFT_0533 0.20 -2.2 528,265 + EX28DRAFT_0533 0.29 -3.3 528,266 - EX28DRAFT_0533 0.29 -2.4 528,301 - EX28DRAFT_0533 0.35 -1.0 528,301 - EX28DRAFT_0533 0.35 -2.6 528,592 + EX28DRAFT_0533 0.82 -4.4 528,593 - EX28DRAFT_0533 0.82 -0.5 528,593 - EX28DRAFT_0533 0.82 -1.5 528,593 - EX28DRAFT_0533 0.82 +0.0 528,593 - EX28DRAFT_0533 0.82 +0.8 528,593 - EX28DRAFT_0533 0.82 -2.5 528,595 - EX28DRAFT_0533 0.83 -3.6 528,731 + -4.1 528,750 + -4.0 528,751 - -2.0 528,751 - -1.7 529,053 - EX28DRAFT_0534 0.46 -3.0 529,074 - EX28DRAFT_0534 0.49 -1.8 529,098 + EX28DRAFT_0534 0.52 -2.8 529,099 - EX28DRAFT_0534 0.52 -2.9 529,177 - EX28DRAFT_0534 0.62 +0.0 529,322 + EX28DRAFT_0534 0.81 -1.2 529,323 - EX28DRAFT_0534 0.81 -3.2 529,435 + +0.0 529,450 + -0.5 529,450 + -3.9 529,450 + -1.8 529,451 - -3.1 529,451 - -2.3 529,490 + -1.7 529,566 + EX28DRAFT_0535 0.15 -0.3 529,566 + EX28DRAFT_0535 0.15 -0.7 529,566 + EX28DRAFT_0535 0.15 -0.8 529,567 - EX28DRAFT_0535 0.16 -4.6 530,055 + EX28DRAFT_0535 0.82 +1.0 530,055 + EX28DRAFT_0535 0.82 -1.1 530,157 - -2.3 530,184 - -0.6 530,305 - EX28DRAFT_0536 0.20 -2.4 530,346 + EX28DRAFT_0536 0.27 +0.0
Or see this region's nucleotide sequence