Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0459 and EX28DRAFT_0460 are separated by 300 nucleotides EX28DRAFT_0460 and EX28DRAFT_0461 are separated by 136 nucleotides
EX28DRAFT_0459: EX28DRAFT_0459 - Superfamily I DNA and RNA helicases, at 443,269 to 445,050
_0459
EX28DRAFT_0460: EX28DRAFT_0460 - hypothetical protein, at 445,351 to 445,593
_0460
EX28DRAFT_0461: EX28DRAFT_0461 - tRNA_anti-like, at 445,730 to 446,512
_0461
Position (kb) 445
446 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 444.528 kb on + strand, within EX28DRAFT_0459 at 444.529 kb on - strand, within EX28DRAFT_0459 at 444.598 kb on + strand, within EX28DRAFT_0459 at 444.710 kb on + strand, within EX28DRAFT_0459 at 444.739 kb on + strand, within EX28DRAFT_0459 at 444.740 kb on - strand, within EX28DRAFT_0459 at 444.740 kb on - strand, within EX28DRAFT_0459 at 444.740 kb on - strand, within EX28DRAFT_0459 at 444.740 kb on - strand, within EX28DRAFT_0459 at 444.744 kb on + strand, within EX28DRAFT_0459 at 444.919 kb on + strand at 444.920 kb on - strand at 444.923 kb on + strand at 444.923 kb on + strand at 444.924 kb on - strand at 444.924 kb on - strand at 444.997 kb on + strand at 444.997 kb on + strand at 444.999 kb on + strand at 445.000 kb on - strand at 445.000 kb on - strand at 445.036 kb on + strand at 445.037 kb on - strand at 445.061 kb on + strand at 445.066 kb on + strand at 445.230 kb on - strand at 445.230 kb on - strand at 445.243 kb on - strand at 445.288 kb on - strand at 445.293 kb on + strand at 445.332 kb on + strand at 445.333 kb on - strand at 445.333 kb on - strand at 445.333 kb on - strand at 445.336 kb on + strand at 445.336 kb on + strand at 445.384 kb on - strand, within EX28DRAFT_0460 at 445.384 kb on - strand, within EX28DRAFT_0460 at 445.416 kb on + strand, within EX28DRAFT_0460 at 445.417 kb on - strand, within EX28DRAFT_0460 at 445.424 kb on + strand, within EX28DRAFT_0460 at 445.425 kb on - strand, within EX28DRAFT_0460 at 445.425 kb on - strand, within EX28DRAFT_0460 at 445.428 kb on + strand, within EX28DRAFT_0460 at 445.428 kb on + strand, within EX28DRAFT_0460 at 445.429 kb on - strand, within EX28DRAFT_0460 at 445.431 kb on + strand, within EX28DRAFT_0460 at 445.431 kb on + strand, within EX28DRAFT_0460 at 445.496 kb on - strand, within EX28DRAFT_0460 at 445.541 kb on - strand, within EX28DRAFT_0460 at 445.550 kb on + strand, within EX28DRAFT_0460 at 445.550 kb on + strand, within EX28DRAFT_0460 at 445.551 kb on - strand, within EX28DRAFT_0460 at 445.551 kb on - strand, within EX28DRAFT_0460 at 445.741 kb on + strand at 445.823 kb on + strand, within EX28DRAFT_0461 at 445.835 kb on + strand, within EX28DRAFT_0461 at 445.835 kb on + strand, within EX28DRAFT_0461 at 445.836 kb on - strand, within EX28DRAFT_0461 at 445.836 kb on - strand, within EX28DRAFT_0461 at 445.836 kb on - strand, within EX28DRAFT_0461 at 445.873 kb on - strand, within EX28DRAFT_0461 at 445.965 kb on - strand, within EX28DRAFT_0461 at 445.977 kb on - strand, within EX28DRAFT_0461 at 445.984 kb on + strand, within EX28DRAFT_0461 at 445.985 kb on - strand, within EX28DRAFT_0461 at 445.985 kb on - strand, within EX28DRAFT_0461 at 445.985 kb on - strand, within EX28DRAFT_0461 at 446.207 kb on + strand, within EX28DRAFT_0461 at 446.240 kb on + strand, within EX28DRAFT_0461 at 446.284 kb on + strand, within EX28DRAFT_0461 at 446.285 kb on - strand, within EX28DRAFT_0461 at 446.316 kb on + strand, within EX28DRAFT_0461 at 446.316 kb on + strand, within EX28DRAFT_0461 at 446.317 kb on - strand, within EX28DRAFT_0461 at 446.317 kb on - strand, within EX28DRAFT_0461 at 446.347 kb on + strand, within EX28DRAFT_0461 at 446.500 kb on + strand at 446.500 kb on + strand at 446.506 kb on + strand at 446.552 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 444,528 + EX28DRAFT_0459 0.71 +0.6 444,529 - EX28DRAFT_0459 0.71 +0.2 444,598 + EX28DRAFT_0459 0.75 +0.3 444,710 + EX28DRAFT_0459 0.81 +0.3 444,739 + EX28DRAFT_0459 0.82 -0.2 444,740 - EX28DRAFT_0459 0.83 -0.7 444,740 - EX28DRAFT_0459 0.83 +0.9 444,740 - EX28DRAFT_0459 0.83 +0.5 444,740 - EX28DRAFT_0459 0.83 -0.4 444,744 + EX28DRAFT_0459 0.83 -1.5 444,919 + -0.3 444,920 - -0.6 444,923 + +0.4 444,923 + +0.5 444,924 - +0.1 444,924 - -0.8 444,997 + -0.5 444,997 + -0.4 444,999 + -1.0 445,000 - -0.0 445,000 - -0.8 445,036 + -1.3 445,037 - -1.8 445,061 + -0.3 445,066 + -0.1 445,230 - -0.4 445,230 - -2.4 445,243 - +0.5 445,288 - +0.1 445,293 + +0.0 445,332 + +0.3 445,333 - +0.1 445,333 - -3.9 445,333 - +2.2 445,336 + -0.2 445,336 + -0.4 445,384 - EX28DRAFT_0460 0.14 +2.1 445,384 - EX28DRAFT_0460 0.14 +1.1 445,416 + EX28DRAFT_0460 0.27 +1.6 445,417 - EX28DRAFT_0460 0.27 -0.5 445,424 + EX28DRAFT_0460 0.30 -0.1 445,425 - EX28DRAFT_0460 0.30 +2.7 445,425 - EX28DRAFT_0460 0.30 -3.2 445,428 + EX28DRAFT_0460 0.32 -1.1 445,428 + EX28DRAFT_0460 0.32 +0.7 445,429 - EX28DRAFT_0460 0.32 +0.1 445,431 + EX28DRAFT_0460 0.33 +0.2 445,431 + EX28DRAFT_0460 0.33 -0.3 445,496 - EX28DRAFT_0460 0.60 -0.7 445,541 - EX28DRAFT_0460 0.78 +0.0 445,550 + EX28DRAFT_0460 0.82 -0.6 445,550 + EX28DRAFT_0460 0.82 -0.0 445,551 - EX28DRAFT_0460 0.82 +0.9 445,551 - EX28DRAFT_0460 0.82 +0.2 445,741 + -0.6 445,823 + EX28DRAFT_0461 0.12 +1.3 445,835 + EX28DRAFT_0461 0.13 +0.4 445,835 + EX28DRAFT_0461 0.13 +0.8 445,836 - EX28DRAFT_0461 0.14 +0.0 445,836 - EX28DRAFT_0461 0.14 +0.1 445,836 - EX28DRAFT_0461 0.14 -0.6 445,873 - EX28DRAFT_0461 0.18 +0.4 445,965 - EX28DRAFT_0461 0.30 +1.1 445,977 - EX28DRAFT_0461 0.32 -0.5 445,984 + EX28DRAFT_0461 0.32 -0.2 445,985 - EX28DRAFT_0461 0.33 +1.0 445,985 - EX28DRAFT_0461 0.33 -0.2 445,985 - EX28DRAFT_0461 0.33 +1.8 446,207 + EX28DRAFT_0461 0.61 -0.5 446,240 + EX28DRAFT_0461 0.65 -0.7 446,284 + EX28DRAFT_0461 0.71 +0.2 446,285 - EX28DRAFT_0461 0.71 -1.1 446,316 + EX28DRAFT_0461 0.75 +0.1 446,316 + EX28DRAFT_0461 0.75 +0.1 446,317 - EX28DRAFT_0461 0.75 -0.8 446,317 - EX28DRAFT_0461 0.75 +2.8 446,347 + EX28DRAFT_0461 0.79 -0.3 446,500 + +1.1 446,500 + +0.0 446,506 + -0.1 446,552 + -0.2
Or see this region's nucleotide sequence