Strain Fitness in Escherichia coli BW25113 around b4153

Experiment: D,L-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntampC and frdD are separated by 62 nucleotidesfrdD and frdC are separated by 10 nucleotidesfrdC and frdB are separated by 10 nucleotidesfrdB and frdA overlap by 8 nucleotides b4150: ampC - beta-lactamase/D-alanine carboxypeptidase (NCBI), at 4,375,834 to 4,376,967 ampC b4151: frdD - fumarate reductase subunit D (NCBI), at 4,377,030 to 4,377,389 frdD b4152: frdC - fumarate reductase subunit C (NCBI), at 4,377,400 to 4,377,795 frdC b4153: frdB - fumarate reductase (anaerobic), Fe-S subunit (NCBI), at 4,377,806 to 4,378,540 frdB b4154: frdA - fumarate reductase (NCBI), at 4,378,533 to 4,380,341 frdA Position (kb) 4377 4378 4379Strain fitness (log2 ratio) -2 -1 0 1 2at 4376.982 kb on + strandat 4377.019 kb on - strandat 4377.043 kb on + strandat 4377.043 kb on + strandat 4377.078 kb on - strand, within frdDat 4377.078 kb on - strand, within frdDat 4377.126 kb on + strand, within frdDat 4377.126 kb on + strand, within frdDat 4377.155 kb on - strand, within frdDat 4377.155 kb on - strand, within frdDat 4377.320 kb on + strand, within frdDat 4377.320 kb on + strand, within frdDat 4377.401 kb on + strandat 4377.401 kb on + strandat 4377.401 kb on + strandat 4377.404 kb on - strandat 4377.404 kb on - strandat 4377.406 kb on - strandat 4377.409 kb on - strandat 4377.409 kb on - strandat 4377.409 kb on - strandat 4377.446 kb on - strand, within frdCat 4377.446 kb on - strand, within frdCat 4377.550 kb on + strand, within frdCat 4377.576 kb on + strand, within frdCat 4377.777 kb on + strandat 4377.794 kb on + strandat 4377.794 kb on + strandat 4377.812 kb on - strandat 4377.812 kb on - strandat 4377.812 kb on - strandat 4377.889 kb on + strand, within frdBat 4377.889 kb on - strand, within frdBat 4377.909 kb on - strand, within frdBat 4377.909 kb on - strand, within frdBat 4377.919 kb on + strand, within frdBat 4377.939 kb on - strand, within frdBat 4377.966 kb on - strand, within frdBat 4377.972 kb on + strand, within frdBat 4377.976 kb on + strand, within frdBat 4377.980 kb on - strand, within frdBat 4377.990 kb on - strand, within frdBat 4378.039 kb on + strand, within frdBat 4378.039 kb on + strand, within frdBat 4378.079 kb on - strand, within frdBat 4378.079 kb on - strand, within frdBat 4378.106 kb on - strand, within frdBat 4378.134 kb on + strand, within frdBat 4378.134 kb on + strand, within frdBat 4378.161 kb on + strand, within frdBat 4378.261 kb on - strand, within frdBat 4378.265 kb on - strand, within frdBat 4378.272 kb on + strand, within frdBat 4378.272 kb on + strand, within frdBat 4378.301 kb on + strand, within frdBat 4378.393 kb on + strand, within frdBat 4378.403 kb on + strand, within frdBat 4378.427 kb on - strand, within frdBat 4378.453 kb on - strand, within frdBat 4378.454 kb on - strand, within frdBat 4378.475 kb on - strandat 4378.475 kb on - strandat 4378.646 kb on + strandat 4378.646 kb on + strandat 4378.665 kb on - strandat 4378.699 kb on - strandat 4378.699 kb on - strandat 4378.720 kb on + strand, within frdAat 4378.726 kb on + strand, within frdAat 4378.726 kb on + strand, within frdAat 4378.740 kb on + strand, within frdAat 4378.758 kb on - strand, within frdAat 4378.768 kb on - strand, within frdAat 4378.768 kb on - strand, within frdAat 4378.777 kb on - strand, within frdAat 4378.777 kb on - strand, within frdAat 4378.789 kb on - strand, within frdAat 4378.789 kb on - strand, within frdAat 4378.800 kb on + strand, within frdAat 4378.800 kb on + strand, within frdAat 4378.800 kb on + strand, within frdAat 4378.800 kb on + strand, within frdAat 4378.809 kb on - strand, within frdAat 4378.825 kb on + strand, within frdAat 4378.825 kb on + strand, within frdAat 4378.825 kb on - strand, within frdAat 4378.825 kb on - strand, within frdAat 4378.825 kb on - strand, within frdAat 4378.833 kb on - strand, within frdAat 4378.840 kb on + strand, within frdAat 4378.850 kb on + strand, within frdAat 4378.858 kb on - strand, within frdAat 4378.903 kb on - strand, within frdAat 4378.903 kb on - strand, within frdAat 4378.906 kb on - strand, within frdAat 4378.935 kb on - strand, within frdAat 4378.935 kb on - strand, within frdAat 4378.956 kb on - strand, within frdAat 4379.115 kb on - strand, within frdAat 4379.179 kb on - strand, within frdAat 4379.181 kb on + strand, within frdAat 4379.181 kb on + strand, within frdAat 4379.186 kb on + strand, within frdAat 4379.186 kb on + strand, within frdAat 4379.199 kb on + strand, within frdAat 4379.199 kb on - strand, within frdAat 4379.208 kb on - strand, within frdAat 4379.271 kb on - strand, within frdAat 4379.271 kb on - strand, within frdAat 4379.271 kb on - strand, within frdAat 4379.279 kb on - strand, within frdAat 4379.285 kb on + strand, within frdAat 4379.289 kb on + strand, within frdAat 4379.354 kb on - strand, within frdAat 4379.410 kb on + strand, within frdAat 4379.438 kb on - strand, within frdAat 4379.445 kb on - strand, within frdAat 4379.445 kb on - strand, within frdAat 4379.526 kb on + strand, within frdA

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Lactate (C)
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4,376,982 + -1.5
4,377,019 - -1.0
4,377,043 + -0.9
4,377,043 + +0.4
4,377,078 - frdD b4151 0.13 +2.0
4,377,078 - frdD b4151 0.13 +1.7
4,377,126 + frdD b4151 0.27 -0.9
4,377,126 + frdD b4151 0.27 +0.1
4,377,155 - frdD b4151 0.35 +0.2
4,377,155 - frdD b4151 0.35 -0.4
4,377,320 + frdD b4151 0.81 -0.2
4,377,320 + frdD b4151 0.81 -0.3
4,377,401 + -0.6
4,377,401 + -0.2
4,377,401 + -0.2
4,377,404 - -0.3
4,377,404 - +0.7
4,377,406 - +0.2
4,377,409 - +0.1
4,377,409 - +0.8
4,377,409 - +0.3
4,377,446 - frdC b4152 0.12 +0.8
4,377,446 - frdC b4152 0.12 -0.0
4,377,550 + frdC b4152 0.38 +0.5
4,377,576 + frdC b4152 0.44 -1.1
4,377,777 + +0.0
4,377,794 + +0.6
4,377,794 + +0.6
4,377,812 - +0.7
4,377,812 - +0.5
4,377,812 - +0.1
4,377,889 + frdB b4153 0.11 +0.6
4,377,889 - frdB b4153 0.11 +0.5
4,377,909 - frdB b4153 0.14 +0.7
4,377,909 - frdB b4153 0.14 +0.6
4,377,919 + frdB b4153 0.15 +0.3
4,377,939 - frdB b4153 0.18 -1.3
4,377,966 - frdB b4153 0.22 +1.0
4,377,972 + frdB b4153 0.23 -1.0
4,377,976 + frdB b4153 0.23 -0.3
4,377,980 - frdB b4153 0.24 -1.0
4,377,990 - frdB b4153 0.25 +0.1
4,378,039 + frdB b4153 0.32 -0.2
4,378,039 + frdB b4153 0.32 -0.9
4,378,079 - frdB b4153 0.37 -0.4
4,378,079 - frdB b4153 0.37 +0.1
4,378,106 - frdB b4153 0.41 +0.1
4,378,134 + frdB b4153 0.45 +0.3
4,378,134 + frdB b4153 0.45 +0.4
4,378,161 + frdB b4153 0.48 +0.2
4,378,261 - frdB b4153 0.62 +1.7
4,378,265 - frdB b4153 0.62 -0.4
4,378,272 + frdB b4153 0.63 -0.4
4,378,272 + frdB b4153 0.63 +0.3
4,378,301 + frdB b4153 0.67 +0.2
4,378,393 + frdB b4153 0.80 -0.0
4,378,403 + frdB b4153 0.81 +1.0
4,378,427 - frdB b4153 0.84 -0.1
4,378,453 - frdB b4153 0.88 +0.9
4,378,454 - frdB b4153 0.88 +1.1
4,378,475 - +0.4
4,378,475 - +1.5
4,378,646 + +0.2
4,378,646 + +1.7
4,378,665 - +0.8
4,378,699 - +0.8
4,378,699 - +0.7
4,378,720 + frdA b4154 0.10 -0.2
4,378,726 + frdA b4154 0.11 +0.2
4,378,726 + frdA b4154 0.11 -0.9
4,378,740 + frdA b4154 0.11 -0.2
4,378,758 - frdA b4154 0.12 -0.5
4,378,768 - frdA b4154 0.13 +0.9
4,378,768 - frdA b4154 0.13 -0.3
4,378,777 - frdA b4154 0.13 -0.4
4,378,777 - frdA b4154 0.13 +0.0
4,378,789 - frdA b4154 0.14 -0.5
4,378,789 - frdA b4154 0.14 +0.1
4,378,800 + frdA b4154 0.15 -0.6
4,378,800 + frdA b4154 0.15 +0.5
4,378,800 + frdA b4154 0.15 -0.7
4,378,800 + frdA b4154 0.15 -0.2
4,378,809 - frdA b4154 0.15 -0.2
4,378,825 + frdA b4154 0.16 -0.7
4,378,825 + frdA b4154 0.16 +0.0
4,378,825 - frdA b4154 0.16 -0.6
4,378,825 - frdA b4154 0.16 +1.1
4,378,825 - frdA b4154 0.16 -0.4
4,378,833 - frdA b4154 0.17 -2.2
4,378,840 + frdA b4154 0.17 -1.0
4,378,850 + frdA b4154 0.18 +0.2
4,378,858 - frdA b4154 0.18 +0.4
4,378,903 - frdA b4154 0.20 +0.0
4,378,903 - frdA b4154 0.20 +0.6
4,378,906 - frdA b4154 0.21 +0.4
4,378,935 - frdA b4154 0.22 +0.7
4,378,935 - frdA b4154 0.22 +1.0
4,378,956 - frdA b4154 0.23 -0.0
4,379,115 - frdA b4154 0.32 +0.1
4,379,179 - frdA b4154 0.36 +0.3
4,379,181 + frdA b4154 0.36 +0.1
4,379,181 + frdA b4154 0.36 -0.6
4,379,186 + frdA b4154 0.36 -1.5
4,379,186 + frdA b4154 0.36 -0.9
4,379,199 + frdA b4154 0.37 +0.2
4,379,199 - frdA b4154 0.37 +1.6
4,379,208 - frdA b4154 0.37 +0.7
4,379,271 - frdA b4154 0.41 +1.7
4,379,271 - frdA b4154 0.41 +1.4
4,379,271 - frdA b4154 0.41 -1.4
4,379,279 - frdA b4154 0.41 -0.0
4,379,285 + frdA b4154 0.42 +0.1
4,379,289 + frdA b4154 0.42 +0.2
4,379,354 - frdA b4154 0.45 +1.8
4,379,410 + frdA b4154 0.48 +1.3
4,379,438 - frdA b4154 0.50 -0.0
4,379,445 - frdA b4154 0.50 +0.9
4,379,445 - frdA b4154 0.50 +0.1
4,379,526 + frdA b4154 0.55 +0.1

Or see this region's nucleotide sequence