Strain Fitness in Escherichia coli BW25113 around b1779

Experiment: D,L-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb1777 and yeaA are separated by 41 nucleotidesyeaA and gapA are separated by 341 nucleotidesgapA and yeaD are separated by 83 nucleotides b1777: b1777 - orf, hypothetical protein (VIMSS), at 1,859,726 to 1,859,998 b1777 b1778: yeaA - methionine sulfoxide reductase B (NCBI), at 1,860,040 to 1,860,453 yeaA b1779: gapA - glyceraldehyde-3-phosphate dehydrogenase (NCBI), at 1,860,795 to 1,861,790 gapA b1780: yeaD - orf, hypothetical protein (VIMSS), at 1,861,874 to 1,862,758 yeaD Position (kb) 1860 1861 1862Strain fitness (log2 ratio) -1 0 1at 1859.799 kb on + strand, within b1777at 1859.816 kb on - strand, within b1777at 1859.972 kb on - strandat 1860.088 kb on + strand, within yeaAat 1860.088 kb on + strand, within yeaAat 1860.321 kb on + strand, within yeaAat 1861.874 kb on - strandat 1861.874 kb on - strandat 1861.922 kb on + strandat 1862.020 kb on - strand, within yeaDat 1862.020 kb on - strand, within yeaDat 1862.069 kb on - strand, within yeaDat 1862.153 kb on + strand, within yeaDat 1862.434 kb on + strand, within yeaDat 1862.442 kb on - strand, within yeaDat 1862.545 kb on + strand, within yeaDat 1862.658 kb on - strand, within yeaDat 1862.691 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Lactate (C)
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1,859,799 + b1777 b1777 0.27 +1.2
1,859,816 - b1777 b1777 0.33 +0.7
1,859,972 - -0.1
1,860,088 + yeaA b1778 0.12 +0.5
1,860,088 + yeaA b1778 0.12 -0.4
1,860,321 + yeaA b1778 0.68 +0.9
1,861,874 - +0.5
1,861,874 - +0.2
1,861,922 + +0.9
1,862,020 - yeaD b1780 0.16 +0.5
1,862,020 - yeaD b1780 0.16 -0.5
1,862,069 - yeaD b1780 0.22 -0.4
1,862,153 + yeaD b1780 0.32 -0.7
1,862,434 + yeaD b1780 0.63 -0.1
1,862,442 - yeaD b1780 0.64 -0.2
1,862,545 + yeaD b1780 0.76 -0.1
1,862,658 - yeaD b1780 0.89 -0.1
1,862,691 - -0.3

Or see this region's nucleotide sequence