Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_2025

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_2023 and PfGW456L13_2024 overlap by 4 nucleotidesPfGW456L13_2024 and PfGW456L13_2025 are separated by 114 nucleotidesPfGW456L13_2025 and PfGW456L13_2026 are separated by 89 nucleotidesPfGW456L13_2026 and PfGW456L13_2027 overlap by 1 nucleotides PfGW456L13_2023 - Flavoprotein WrbA, at 2,226,153 to 2,226,749 _2023 PfGW456L13_2024 - Arsenate reductase (EC 1.20.4.1), at 2,226,746 to 2,227,099 _2024 PfGW456L13_2025 - Cytochrome c-type biogenesis protein ResA, at 2,227,214 to 2,227,681 _2025 PfGW456L13_2026 - Heat shock protein, at 2,227,771 to 2,228,175 _2026 PfGW456L13_2027 - FIG00953240: hypothetical protein, at 2,228,175 to 2,228,609 _2027 Position (kb) 2227 2228Strain fitness (log2 ratio) -3 -2 -1 0 1at 2226.317 kb on - strand, within PfGW456L13_2023at 2226.481 kb on + strand, within PfGW456L13_2023at 2226.481 kb on + strand, within PfGW456L13_2023at 2226.481 kb on + strand, within PfGW456L13_2023at 2226.568 kb on + strand, within PfGW456L13_2023at 2226.568 kb on + strandat 2226.568 kb on + strandat 2226.718 kb on + strandat 2226.719 kb on - strandat 2226.719 kb on - strandat 2226.719 kb on - strandat 2226.721 kb on + strandat 2226.721 kb on + strandat 2226.722 kb on - strandat 2226.722 kb on - strandat 2226.736 kb on + strandat 2226.736 kb on + strandat 2226.736 kb on + strandat 2226.737 kb on - strandat 2226.737 kb on - strandat 2226.737 kb on - strandat 2226.737 kb on - strandat 2226.903 kb on + strand, within PfGW456L13_2024at 2226.904 kb on - strand, within PfGW456L13_2024at 2226.904 kb on - strand, within PfGW456L13_2024at 2226.904 kb on - strand, within PfGW456L13_2024at 2226.904 kb on - strand, within PfGW456L13_2024at 2227.080 kb on + strandat 2227.080 kb on + strandat 2227.080 kb on + strandat 2227.081 kb on - strandat 2227.119 kb on + strandat 2227.132 kb on - strandat 2227.136 kb on + strandat 2227.136 kb on + strandat 2227.136 kb on + strandat 2227.136 kb on + strandat 2227.136 kb on + strandat 2227.136 kb on + strandat 2227.137 kb on - strandat 2227.137 kb on - strandat 2227.164 kb on + strandat 2227.165 kb on + strandat 2227.165 kb on - strandat 2227.283 kb on + strand, within PfGW456L13_2025at 2227.283 kb on + strand, within PfGW456L13_2025at 2227.283 kb on + strand, within PfGW456L13_2025at 2227.284 kb on - strand, within PfGW456L13_2025at 2227.352 kb on + strand, within PfGW456L13_2025at 2227.353 kb on - strand, within PfGW456L13_2025at 2227.353 kb on - strand, within PfGW456L13_2025at 2227.580 kb on + strand, within PfGW456L13_2025at 2227.580 kb on + strand, within PfGW456L13_2025at 2227.580 kb on + strand, within PfGW456L13_2025at 2227.580 kb on + strand, within PfGW456L13_2025at 2227.580 kb on + strand, within PfGW456L13_2025at 2227.581 kb on - strand, within PfGW456L13_2025at 2227.581 kb on - strand, within PfGW456L13_2025at 2227.581 kb on - strand, within PfGW456L13_2025at 2227.581 kb on - strand, within PfGW456L13_2025at 2227.581 kb on - strand, within PfGW456L13_2025at 2227.581 kb on - strand, within PfGW456L13_2025at 2227.581 kb on - strand, within PfGW456L13_2025at 2227.581 kb on - strand, within PfGW456L13_2025at 2227.581 kb on - strand, within PfGW456L13_2025at 2227.581 kb on - strand, within PfGW456L13_2025at 2227.825 kb on + strand, within PfGW456L13_2026at 2227.826 kb on - strand, within PfGW456L13_2026at 2227.970 kb on + strand, within PfGW456L13_2026at 2227.971 kb on - strand, within PfGW456L13_2026at 2228.007 kb on - strand, within PfGW456L13_2026at 2228.049 kb on - strand, within PfGW456L13_2026at 2228.087 kb on + strand, within PfGW456L13_2026at 2228.087 kb on + strand, within PfGW456L13_2026at 2228.087 kb on + strand, within PfGW456L13_2026at 2228.087 kb on + strand, within PfGW456L13_2026at 2228.088 kb on - strand, within PfGW456L13_2026at 2228.088 kb on - strand, within PfGW456L13_2026at 2228.088 kb on - strand, within PfGW456L13_2026at 2228.088 kb on - strand, within PfGW456L13_2026at 2228.088 kb on - strand, within PfGW456L13_2026at 2228.622 kb on + strandat 2228.622 kb on + strandat 2228.622 kb on + strandat 2228.623 kb on - strandat 2228.623 kb on - strandat 2228.632 kb on - strandat 2228.636 kb on + strandat 2228.636 kb on + strandat 2228.637 kb on - strandat 2228.637 kb on - strandat 2228.649 kb on + strandat 2228.650 kb on - strandat 2228.650 kb on - strandat 2228.650 kb on - strandat 2228.667 kb on + strandat 2228.667 kb on + strandat 2228.667 kb on + strandat 2228.668 kb on - strandat 2228.668 kb on - strandat 2228.668 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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2,226,317 - PfGW456L13_2023 0.27 -0.4
2,226,481 + PfGW456L13_2023 0.55 -1.0
2,226,481 + PfGW456L13_2023 0.55 +0.8
2,226,481 + PfGW456L13_2023 0.55 -0.1
2,226,568 + PfGW456L13_2023 0.70 -0.4
2,226,568 + +0.9
2,226,568 + -0.7
2,226,718 + -1.9
2,226,719 - -0.1
2,226,719 - -1.3
2,226,719 - +0.8
2,226,721 + +0.2
2,226,721 + +0.0
2,226,722 - -0.5
2,226,722 - +0.9
2,226,736 + +0.1
2,226,736 + -0.1
2,226,736 + +0.1
2,226,737 - -0.1
2,226,737 - +0.0
2,226,737 - -0.0
2,226,737 - +0.0
2,226,903 + PfGW456L13_2024 0.44 +0.3
2,226,904 - PfGW456L13_2024 0.45 +0.0
2,226,904 - PfGW456L13_2024 0.45 -0.2
2,226,904 - PfGW456L13_2024 0.45 -0.2
2,226,904 - PfGW456L13_2024 0.45 +0.3
2,227,080 + -0.2
2,227,080 + -0.1
2,227,080 + -0.9
2,227,081 - -1.8
2,227,119 + -0.1
2,227,132 - -1.9
2,227,136 + -1.0
2,227,136 + -0.5
2,227,136 + -0.0
2,227,136 + -0.7
2,227,136 + -1.5
2,227,136 + -2.6
2,227,137 - +0.9
2,227,137 - -0.5
2,227,164 + +0.3
2,227,165 + -1.5
2,227,165 - +0.4
2,227,283 + PfGW456L13_2025 0.15 -2.9
2,227,283 + PfGW456L13_2025 0.15 -1.4
2,227,283 + PfGW456L13_2025 0.15 -0.9
2,227,284 - PfGW456L13_2025 0.15 -1.7
2,227,352 + PfGW456L13_2025 0.29 -2.0
2,227,353 - PfGW456L13_2025 0.30 -0.5
2,227,353 - PfGW456L13_2025 0.30 -0.6
2,227,580 + PfGW456L13_2025 0.78 -1.8
2,227,580 + PfGW456L13_2025 0.78 -2.3
2,227,580 + PfGW456L13_2025 0.78 -3.2
2,227,580 + PfGW456L13_2025 0.78 -0.1
2,227,580 + PfGW456L13_2025 0.78 -1.5
2,227,581 - PfGW456L13_2025 0.78 -0.1
2,227,581 - PfGW456L13_2025 0.78 -1.7
2,227,581 - PfGW456L13_2025 0.78 -0.3
2,227,581 - PfGW456L13_2025 0.78 -1.1
2,227,581 - PfGW456L13_2025 0.78 -1.1
2,227,581 - PfGW456L13_2025 0.78 -1.2
2,227,581 - PfGW456L13_2025 0.78 -2.3
2,227,581 - PfGW456L13_2025 0.78 -1.9
2,227,581 - PfGW456L13_2025 0.78 -1.0
2,227,581 - PfGW456L13_2025 0.78 -0.9
2,227,825 + PfGW456L13_2026 0.13 -0.4
2,227,826 - PfGW456L13_2026 0.14 +0.9
2,227,970 + PfGW456L13_2026 0.49 +0.5
2,227,971 - PfGW456L13_2026 0.49 +0.3
2,228,007 - PfGW456L13_2026 0.58 +0.1
2,228,049 - PfGW456L13_2026 0.69 +0.4
2,228,087 + PfGW456L13_2026 0.78 +0.5
2,228,087 + PfGW456L13_2026 0.78 +1.6
2,228,087 + PfGW456L13_2026 0.78 -0.7
2,228,087 + PfGW456L13_2026 0.78 -0.4
2,228,088 - PfGW456L13_2026 0.78 +0.4
2,228,088 - PfGW456L13_2026 0.78 +0.4
2,228,088 - PfGW456L13_2026 0.78 +0.5
2,228,088 - PfGW456L13_2026 0.78 -0.3
2,228,088 - PfGW456L13_2026 0.78 -0.5
2,228,622 + +0.8
2,228,622 + +0.1
2,228,622 + +0.2
2,228,623 - +1.1
2,228,623 - -0.7
2,228,632 - +0.1
2,228,636 + -0.0
2,228,636 + -0.4
2,228,637 - -1.3
2,228,637 - +0.2
2,228,649 + +0.2
2,228,650 - -0.4
2,228,650 - +0.7
2,228,650 - -0.2
2,228,667 + +0.9
2,228,667 + +0.6
2,228,667 + +0.2
2,228,668 - +0.6
2,228,668 - +0.1
2,228,668 - -0.5

Or see this region's nucleotide sequence