Experiment: succinate (C); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS02980 and PFLU_RS02985 overlap by 1 nucleotides PFLU_RS02985 and PFLU_RS02990 overlap by 1 nucleotides PFLU_RS02990 and PFLU_RS02995 are separated by 236 nucleotides
PFLU_RS02980: PFLU_RS02980 - precorrin-8X methylmutase, at 685,950 to 686,576
_RS02980
PFLU_RS02985: PFLU_RS02985 - precorrin-2 C(20)-methyltransferase, at 686,576 to 687,307
_RS02985
PFLU_RS02990: PFLU_RS02990 - precorrin-3B C(17)-methyltransferase, at 687,307 to 688,944
_RS02990
PFLU_RS02995: PFLU_RS02995 - hypothetical protein, at 689,181 to 689,960
_RS02995
Position (kb) 687
688
689 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 686.373 kb on + strand, within PFLU_RS02980 at 686.373 kb on + strand, within PFLU_RS02980 at 686.681 kb on + strand, within PFLU_RS02985 at 686.682 kb on - strand, within PFLU_RS02985 at 686.768 kb on + strand, within PFLU_RS02985 at 686.769 kb on - strand, within PFLU_RS02985 at 686.788 kb on - strand, within PFLU_RS02985 at 686.788 kb on - strand, within PFLU_RS02985 at 686.807 kb on + strand, within PFLU_RS02985 at 686.807 kb on + strand, within PFLU_RS02985 at 686.807 kb on + strand, within PFLU_RS02985 at 686.807 kb on + strand, within PFLU_RS02985 at 686.808 kb on - strand, within PFLU_RS02985 at 686.831 kb on + strand, within PFLU_RS02985 at 686.831 kb on + strand, within PFLU_RS02985 at 686.915 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.958 kb on - strand, within PFLU_RS02985 at 687.191 kb on + strand, within PFLU_RS02985 at 687.191 kb on + strand, within PFLU_RS02985 at 687.191 kb on + strand, within PFLU_RS02985 at 687.191 kb on + strand, within PFLU_RS02985 at 687.191 kb on + strand, within PFLU_RS02985 at 687.191 kb on + strand, within PFLU_RS02985 at 687.191 kb on + strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.263 kb on + strand at 687.264 kb on - strand at 687.264 kb on - strand at 687.264 kb on - strand at 687.264 kb on - strand at 687.430 kb on + strand at 687.431 kb on - strand at 687.431 kb on - strand at 687.431 kb on - strand at 687.431 kb on - strand at 687.431 kb on - strand at 687.463 kb on + strand at 687.504 kb on + strand, within PFLU_RS02990 at 687.504 kb on + strand, within PFLU_RS02990 at 687.673 kb on - strand, within PFLU_RS02990 at 687.914 kb on - strand, within PFLU_RS02990 at 687.914 kb on - strand, within PFLU_RS02990 at 688.282 kb on + strand, within PFLU_RS02990 at 688.282 kb on + strand, within PFLU_RS02990 at 688.282 kb on + strand, within PFLU_RS02990 at 688.283 kb on - strand, within PFLU_RS02990 at 688.666 kb on + strand, within PFLU_RS02990 at 688.667 kb on - strand, within PFLU_RS02990 at 688.667 kb on - strand, within PFLU_RS02990 at 688.667 kb on - strand, within PFLU_RS02990 at 688.667 kb on - strand, within PFLU_RS02990 at 688.906 kb on + strand at 688.907 kb on - strand at 689.135 kb on + strand at 689.135 kb on + strand at 689.136 kb on - strand at 689.136 kb on - strand at 689.146 kb on + strand at 689.146 kb on + strand at 689.146 kb on + strand at 689.146 kb on + strand at 689.146 kb on + strand at 689.146 kb on + strand at 689.146 kb on + strand at 689.146 kb on + strand at 689.146 kb on + strand at 689.146 kb on + strand at 689.146 kb on + strand at 689.146 kb on + strand at 689.146 kb on + strand at 689.146 kb on + strand at 689.147 kb on - strand at 689.147 kb on - strand at 689.147 kb on - strand at 689.147 kb on - strand at 689.147 kb on - strand at 689.210 kb on + strand at 689.210 kb on + strand at 689.210 kb on + strand at 689.238 kb on + strand at 689.276 kb on - strand, within PFLU_RS02995 at 689.367 kb on + strand, within PFLU_RS02995 at 689.367 kb on + strand, within PFLU_RS02995 at 689.367 kb on + strand, within PFLU_RS02995 at 689.367 kb on + strand, within PFLU_RS02995 at 689.367 kb on + strand, within PFLU_RS02995 at 689.368 kb on - strand, within PFLU_RS02995 at 689.368 kb on - strand, within PFLU_RS02995 at 689.368 kb on - strand, within PFLU_RS02995 at 689.368 kb on - strand, within PFLU_RS02995 at 689.368 kb on - strand, within PFLU_RS02995 at 689.368 kb on - strand, within PFLU_RS02995 at 689.368 kb on - strand, within PFLU_RS02995 at 689.376 kb on - strand, within PFLU_RS02995 at 689.382 kb on + strand, within PFLU_RS02995 at 689.383 kb on - strand, within PFLU_RS02995 at 689.383 kb on - strand, within PFLU_RS02995 at 689.400 kb on + strand, within PFLU_RS02995 at 689.401 kb on - strand, within PFLU_RS02995 at 689.401 kb on - strand, within PFLU_RS02995 at 689.457 kb on - strand, within PFLU_RS02995 at 689.457 kb on - strand, within PFLU_RS02995 at 689.582 kb on + strand, within PFLU_RS02995 at 689.583 kb on - strand, within PFLU_RS02995 at 689.583 kb on - strand, within PFLU_RS02995 at 689.695 kb on + strand, within PFLU_RS02995 at 689.696 kb on - strand, within PFLU_RS02995 at 689.769 kb on + strand, within PFLU_RS02995 at 689.769 kb on + strand, within PFLU_RS02995 at 689.769 kb on + strand, within PFLU_RS02995 at 689.769 kb on + strand, within PFLU_RS02995 at 689.769 kb on + strand, within PFLU_RS02995 at 689.770 kb on - strand, within PFLU_RS02995 at 689.770 kb on - strand, within PFLU_RS02995 at 689.770 kb on - strand, within PFLU_RS02995 at 689.770 kb on - strand, within PFLU_RS02995 at 689.770 kb on - strand, within PFLU_RS02995
Per-strain Table
Position Strand Gene LocusTag Fraction succinate (C); with MOPS remove 686,373 + PFLU_RS02980 0.67 -2.5 686,373 + PFLU_RS02980 0.67 -1.3 686,681 + PFLU_RS02985 0.14 -2.4 686,682 - PFLU_RS02985 0.14 +0.3 686,768 + PFLU_RS02985 0.26 -1.2 686,769 - PFLU_RS02985 0.26 -1.4 686,788 - PFLU_RS02985 0.29 -0.3 686,788 - PFLU_RS02985 0.29 -0.3 686,807 + PFLU_RS02985 0.32 -0.1 686,807 + PFLU_RS02985 0.32 +0.4 686,807 + PFLU_RS02985 0.32 -0.4 686,807 + PFLU_RS02985 0.32 -0.6 686,808 - PFLU_RS02985 0.32 -0.1 686,831 + PFLU_RS02985 0.35 -0.1 686,831 + PFLU_RS02985 0.35 -0.9 686,915 + PFLU_RS02985 0.46 -2.2 686,924 + PFLU_RS02985 0.48 -0.5 686,924 + PFLU_RS02985 0.48 -2.1 686,924 + PFLU_RS02985 0.48 -2.2 686,924 + PFLU_RS02985 0.48 -0.4 686,924 + PFLU_RS02985 0.48 -0.4 686,924 + PFLU_RS02985 0.48 -0.3 686,924 + PFLU_RS02985 0.48 +1.8 686,924 + PFLU_RS02985 0.48 -0.9 686,924 + PFLU_RS02985 0.48 +0.1 686,924 + PFLU_RS02985 0.48 -0.9 686,924 + PFLU_RS02985 0.48 -0.7 686,925 - PFLU_RS02985 0.48 -1.1 686,925 - PFLU_RS02985 0.48 +0.4 686,925 - PFLU_RS02985 0.48 +0.1 686,925 - PFLU_RS02985 0.48 -1.0 686,925 - PFLU_RS02985 0.48 -0.6 686,925 - PFLU_RS02985 0.48 -1.3 686,925 - PFLU_RS02985 0.48 -1.7 686,925 - PFLU_RS02985 0.48 -2.7 686,925 - PFLU_RS02985 0.48 -0.6 686,930 + PFLU_RS02985 0.48 -1.2 686,930 + PFLU_RS02985 0.48 -1.4 686,930 + PFLU_RS02985 0.48 -0.7 686,930 + PFLU_RS02985 0.48 +1.5 686,930 + PFLU_RS02985 0.48 -2.9 686,930 + PFLU_RS02985 0.48 -1.2 686,930 + PFLU_RS02985 0.48 -1.0 686,931 - PFLU_RS02985 0.48 +0.5 686,931 - PFLU_RS02985 0.48 -1.4 686,931 - PFLU_RS02985 0.48 -2.6 686,931 - PFLU_RS02985 0.48 -0.1 686,931 - PFLU_RS02985 0.48 +2.3 686,931 - PFLU_RS02985 0.48 -2.1 686,931 - PFLU_RS02985 0.48 +0.6 686,931 - PFLU_RS02985 0.48 -0.9 686,931 - PFLU_RS02985 0.48 -1.1 686,931 - PFLU_RS02985 0.48 -2.2 686,931 - PFLU_RS02985 0.48 -1.3 686,958 - PFLU_RS02985 0.52 -1.4 687,191 + PFLU_RS02985 0.84 -2.0 687,191 + PFLU_RS02985 0.84 -0.7 687,191 + PFLU_RS02985 0.84 -0.4 687,191 + PFLU_RS02985 0.84 -0.1 687,191 + PFLU_RS02985 0.84 +0.3 687,191 + PFLU_RS02985 0.84 -2.8 687,191 + PFLU_RS02985 0.84 +0.2 687,192 - PFLU_RS02985 0.84 +0.9 687,192 - PFLU_RS02985 0.84 +1.1 687,192 - PFLU_RS02985 0.84 -0.4 687,192 - PFLU_RS02985 0.84 -0.2 687,192 - PFLU_RS02985 0.84 -0.7 687,192 - PFLU_RS02985 0.84 -0.9 687,192 - PFLU_RS02985 0.84 -0.7 687,192 - PFLU_RS02985 0.84 -0.7 687,192 - PFLU_RS02985 0.84 -2.3 687,192 - PFLU_RS02985 0.84 +0.4 687,192 - PFLU_RS02985 0.84 -0.5 687,263 + -0.7 687,264 - -0.7 687,264 - +1.9 687,264 - +0.3 687,264 - -0.6 687,430 + -0.3 687,431 - -1.3 687,431 - -0.9 687,431 - -1.5 687,431 - -0.9 687,431 - +0.3 687,463 + -3.3 687,504 + PFLU_RS02990 0.12 -1.3 687,504 + PFLU_RS02990 0.12 -0.8 687,673 - PFLU_RS02990 0.22 -0.6 687,914 - PFLU_RS02990 0.37 -1.7 687,914 - PFLU_RS02990 0.37 -1.7 688,282 + PFLU_RS02990 0.60 -1.0 688,282 + PFLU_RS02990 0.60 -1.3 688,282 + PFLU_RS02990 0.60 -0.7 688,283 - PFLU_RS02990 0.60 -1.0 688,666 + PFLU_RS02990 0.83 -0.4 688,667 - PFLU_RS02990 0.83 -1.4 688,667 - PFLU_RS02990 0.83 +1.3 688,667 - PFLU_RS02990 0.83 -1.9 688,667 - PFLU_RS02990 0.83 -2.2 688,906 + -0.4 688,907 - +0.7 689,135 + -1.1 689,135 + -1.5 689,136 - -0.4 689,136 - +0.0 689,146 + +0.4 689,146 + +1.2 689,146 + +0.4 689,146 + -0.5 689,146 + +1.2 689,146 + -0.1 689,146 + -0.6 689,146 + +0.0 689,146 + +0.7 689,146 + +0.1 689,146 + -1.0 689,146 + +0.3 689,146 + -0.6 689,146 + -1.1 689,147 - -0.3 689,147 - +1.3 689,147 - -0.0 689,147 - +1.7 689,147 - -0.1 689,210 + +0.1 689,210 + -1.6 689,210 + +0.1 689,238 + -0.7 689,276 - PFLU_RS02995 0.12 -1.5 689,367 + PFLU_RS02995 0.24 +0.8 689,367 + PFLU_RS02995 0.24 +0.7 689,367 + PFLU_RS02995 0.24 -1.0 689,367 + PFLU_RS02995 0.24 +0.8 689,367 + PFLU_RS02995 0.24 +0.5 689,368 - PFLU_RS02995 0.24 -0.0 689,368 - PFLU_RS02995 0.24 -0.0 689,368 - PFLU_RS02995 0.24 +0.2 689,368 - PFLU_RS02995 0.24 +0.7 689,368 - PFLU_RS02995 0.24 -0.3 689,368 - PFLU_RS02995 0.24 -0.1 689,368 - PFLU_RS02995 0.24 +0.9 689,376 - PFLU_RS02995 0.25 +0.9 689,382 + PFLU_RS02995 0.26 +0.0 689,383 - PFLU_RS02995 0.26 -1.4 689,383 - PFLU_RS02995 0.26 +1.4 689,400 + PFLU_RS02995 0.28 -0.3 689,401 - PFLU_RS02995 0.28 -0.5 689,401 - PFLU_RS02995 0.28 -1.6 689,457 - PFLU_RS02995 0.35 +0.2 689,457 - PFLU_RS02995 0.35 -0.3 689,582 + PFLU_RS02995 0.51 +0.2 689,583 - PFLU_RS02995 0.52 +2.1 689,583 - PFLU_RS02995 0.52 -0.3 689,695 + PFLU_RS02995 0.66 -1.3 689,696 - PFLU_RS02995 0.66 +0.1 689,769 + PFLU_RS02995 0.75 -1.5 689,769 + PFLU_RS02995 0.75 +0.7 689,769 + PFLU_RS02995 0.75 +0.7 689,769 + PFLU_RS02995 0.75 -1.0 689,769 + PFLU_RS02995 0.75 +3.0 689,770 - PFLU_RS02995 0.76 -0.6 689,770 - PFLU_RS02995 0.76 +1.0 689,770 - PFLU_RS02995 0.76 -0.3 689,770 - PFLU_RS02995 0.76 +0.5 689,770 - PFLU_RS02995 0.76 -1.1
Or see this region's nucleotide sequence