Experiment: succinate (C); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS02445 and PFLU_RS02450 overlap by 10 nucleotides PFLU_RS02450 and PFLU_RS31380 overlap by 71 nucleotides PFLU_RS31380 and PFLU_RS02455 overlap by 17 nucleotides PFLU_RS02455 and PFLU_RS02460 are separated by 206 nucleotides PFLU_RS02460 and PFLU_RS02465 are separated by 45 nucleotides
PFLU_RS02445: PFLU_RS02445 - NUDIX domain-containing protein, at 562,426 to 563,043
_RS02445
PFLU_RS02450: PFLU_RS02450 - DUF1249 domain-containing protein, at 563,034 to 563,483
_RS02450
PFLU_RS31380: PFLU_RS31380 - hypothetical protein, at 563,413 to 563,598
_RS31380
PFLU_RS02455: PFLU_RS02455 - 3',5'-cyclic-AMP phosphodiesterase, at 563,582 to 564,397
_RS02455
PFLU_RS02460: PFLU_RS02460 - alpha/beta fold hydrolase, at 564,604 to 565,212
_RS02460
PFLU_RS02465: PFLU_RS02465 - DNA topoisomerase IV subunit B, at 565,258 to 567,162
_RS02465
Position (kb) 563
564
565 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 562.770 kb on + strand, within PFLU_RS02445 at 562.770 kb on + strand, within PFLU_RS02445 at 562.770 kb on + strand, within PFLU_RS02445 at 562.770 kb on + strand, within PFLU_RS02445 at 562.770 kb on + strand, within PFLU_RS02445 at 562.771 kb on - strand, within PFLU_RS02445 at 562.771 kb on - strand, within PFLU_RS02445 at 562.797 kb on + strand, within PFLU_RS02445 at 562.802 kb on - strand, within PFLU_RS02445 at 562.824 kb on + strand, within PFLU_RS02445 at 562.843 kb on + strand, within PFLU_RS02445 at 562.843 kb on + strand, within PFLU_RS02445 at 562.843 kb on + strand, within PFLU_RS02445 at 562.844 kb on - strand, within PFLU_RS02445 at 562.844 kb on - strand, within PFLU_RS02445 at 562.844 kb on - strand, within PFLU_RS02445 at 563.041 kb on + strand at 563.042 kb on - strand at 563.103 kb on + strand, within PFLU_RS02450 at 563.103 kb on + strand, within PFLU_RS02450 at 563.104 kb on - strand, within PFLU_RS02450 at 563.104 kb on - strand, within PFLU_RS02450 at 563.104 kb on - strand, within PFLU_RS02450 at 563.104 kb on - strand, within PFLU_RS02450 at 563.104 kb on - strand, within PFLU_RS02450 at 563.104 kb on - strand, within PFLU_RS02450 at 563.104 kb on - strand, within PFLU_RS02450 at 563.104 kb on - strand, within PFLU_RS02450 at 563.226 kb on + strand, within PFLU_RS02450 at 563.226 kb on + strand, within PFLU_RS02450 at 563.227 kb on - strand, within PFLU_RS02450 at 563.227 kb on - strand, within PFLU_RS02450 at 563.293 kb on + strand, within PFLU_RS02450 at 563.293 kb on + strand, within PFLU_RS02450 at 563.293 kb on + strand, within PFLU_RS02450 at 563.293 kb on + strand, within PFLU_RS02450 at 563.293 kb on + strand, within PFLU_RS02450 at 563.293 kb on + strand, within PFLU_RS02450 at 563.293 kb on + strand, within PFLU_RS02450 at 563.293 kb on + strand, within PFLU_RS02450 at 563.293 kb on + strand, within PFLU_RS02450 at 563.293 kb on + strand, within PFLU_RS02450 at 563.293 kb on + strand, within PFLU_RS02450 at 563.294 kb on - strand, within PFLU_RS02450 at 563.294 kb on - strand, within PFLU_RS02450 at 563.294 kb on - strand, within PFLU_RS02450 at 563.301 kb on + strand, within PFLU_RS02450 at 563.301 kb on + strand, within PFLU_RS02450 at 563.301 kb on + strand, within PFLU_RS02450 at 563.302 kb on - strand, within PFLU_RS02450 at 563.302 kb on - strand, within PFLU_RS02450 at 563.302 kb on - strand, within PFLU_RS02450 at 563.302 kb on - strand, within PFLU_RS02450 at 563.364 kb on + strand, within PFLU_RS02450 at 563.364 kb on + strand, within PFLU_RS02450 at 563.364 kb on + strand, within PFLU_RS02450 at 563.364 kb on + strand, within PFLU_RS02450 at 563.364 kb on + strand, within PFLU_RS02450 at 563.364 kb on + strand, within PFLU_RS02450 at 563.364 kb on + strand, within PFLU_RS02450 at 563.364 kb on + strand, within PFLU_RS02450 at 563.365 kb on - strand, within PFLU_RS02450 at 563.365 kb on - strand, within PFLU_RS02450 at 563.365 kb on - strand, within PFLU_RS02450 at 563.365 kb on - strand, within PFLU_RS02450 at 563.365 kb on - strand, within PFLU_RS02450 at 563.365 kb on - strand, within PFLU_RS02450 at 563.365 kb on - strand, within PFLU_RS02450 at 563.365 kb on - strand, within PFLU_RS02450 at 563.365 kb on - strand, within PFLU_RS02450 at 563.376 kb on - strand, within PFLU_RS02450 at 563.481 kb on + strand at 563.481 kb on + strand at 563.481 kb on + strand at 563.481 kb on + strand at 563.482 kb on - strand at 563.535 kb on + strand, within PFLU_RS31380 at 563.535 kb on + strand, within PFLU_RS31380 at 563.535 kb on + strand, within PFLU_RS31380 at 563.535 kb on + strand, within PFLU_RS31380 at 563.535 kb on + strand, within PFLU_RS31380 at 563.536 kb on - strand, within PFLU_RS31380 at 563.536 kb on - strand, within PFLU_RS31380 at 563.536 kb on - strand, within PFLU_RS31380 at 563.556 kb on + strand, within PFLU_RS31380 at 563.556 kb on + strand, within PFLU_RS31380 at 563.557 kb on - strand, within PFLU_RS31380 at 563.592 kb on + strand at 563.592 kb on + strand at 563.593 kb on - strand at 563.593 kb on - strand at 563.795 kb on + strand, within PFLU_RS02455 at 563.796 kb on - strand, within PFLU_RS02455 at 563.796 kb on - strand, within PFLU_RS02455 at 564.165 kb on + strand, within PFLU_RS02455 at 564.166 kb on - strand, within PFLU_RS02455 at 564.377 kb on + strand at 564.378 kb on - strand at 564.395 kb on + strand at 564.396 kb on - strand at 564.396 kb on - strand at 564.396 kb on - strand at 564.462 kb on + strand at 564.462 kb on + strand at 564.462 kb on + strand at 564.462 kb on + strand at 564.462 kb on + strand at 564.462 kb on + strand at 564.462 kb on + strand at 564.462 kb on + strand at 564.463 kb on - strand at 564.463 kb on - strand at 564.463 kb on - strand at 564.463 kb on - strand at 564.463 kb on - strand at 564.463 kb on - strand at 564.463 kb on - strand at 564.463 kb on - strand at 564.463 kb on - strand at 564.463 kb on - strand at 564.463 kb on - strand at 564.466 kb on + strand at 564.466 kb on + strand at 564.466 kb on + strand at 564.466 kb on + strand at 564.466 kb on + strand at 564.466 kb on + strand at 564.466 kb on + strand at 564.466 kb on + strand at 564.466 kb on + strand at 564.466 kb on + strand at 564.466 kb on + strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.467 kb on - strand at 564.525 kb on + strand at 564.525 kb on + strand at 564.526 kb on - strand at 564.526 kb on - strand at 564.565 kb on + strand at 564.566 kb on - strand at 564.622 kb on + strand at 564.622 kb on + strand at 564.622 kb on + strand at 564.622 kb on + strand at 564.622 kb on + strand at 564.622 kb on + strand at 564.622 kb on + strand at 564.622 kb on + strand at 564.622 kb on + strand at 564.623 kb on - strand at 564.623 kb on - strand at 564.623 kb on - strand at 564.623 kb on - strand at 564.623 kb on - strand at 564.623 kb on - strand at 564.623 kb on - strand at 564.623 kb on - strand at 564.624 kb on + strand at 564.794 kb on + strand, within PFLU_RS02460 at 564.794 kb on + strand, within PFLU_RS02460 at 564.795 kb on - strand, within PFLU_RS02460 at 564.795 kb on - strand, within PFLU_RS02460 at 564.818 kb on - strand, within PFLU_RS02460 at 564.820 kb on + strand, within PFLU_RS02460 at 564.820 kb on + strand, within PFLU_RS02460 at 564.820 kb on + strand, within PFLU_RS02460 at 564.820 kb on + strand, within PFLU_RS02460 at 564.820 kb on + strand, within PFLU_RS02460 at 564.820 kb on + strand, within PFLU_RS02460 at 564.820 kb on + strand, within PFLU_RS02460 at 564.820 kb on + strand, within PFLU_RS02460 at 564.820 kb on + strand, within PFLU_RS02460 at 564.820 kb on + strand, within PFLU_RS02460 at 564.821 kb on - strand, within PFLU_RS02460 at 564.821 kb on - strand, within PFLU_RS02460 at 564.821 kb on - strand, within PFLU_RS02460 at 564.821 kb on - strand, within PFLU_RS02460 at 564.821 kb on - strand, within PFLU_RS02460 at 564.821 kb on - strand, within PFLU_RS02460 at 564.821 kb on - strand, within PFLU_RS02460 at 564.821 kb on - strand, within PFLU_RS02460 at 564.821 kb on - strand, within PFLU_RS02460 at 564.821 kb on - strand, within PFLU_RS02460 at 564.821 kb on - strand, within PFLU_RS02460 at 564.821 kb on - strand, within PFLU_RS02460 at 564.821 kb on - strand, within PFLU_RS02460 at 564.821 kb on - strand, within PFLU_RS02460 at 565.054 kb on + strand, within PFLU_RS02460 at 565.072 kb on + strand, within PFLU_RS02460 at 565.072 kb on + strand, within PFLU_RS02460 at 565.072 kb on + strand, within PFLU_RS02460 at 565.072 kb on + strand, within PFLU_RS02460 at 565.073 kb on - strand, within PFLU_RS02460 at 565.120 kb on + strand, within PFLU_RS02460 at 565.143 kb on - strand, within PFLU_RS02460 at 565.210 kb on + strand at 565.210 kb on + strand at 565.210 kb on + strand at 565.211 kb on - strand at 565.211 kb on - strand at 565.211 kb on - strand at 565.211 kb on - strand at 565.227 kb on + strand at 565.231 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction succinate (C); with MOPS remove 562,770 + PFLU_RS02445 0.56 +0.1 562,770 + PFLU_RS02445 0.56 +0.4 562,770 + PFLU_RS02445 0.56 -1.5 562,770 + PFLU_RS02445 0.56 +0.8 562,770 + PFLU_RS02445 0.56 +0.4 562,771 - PFLU_RS02445 0.56 -0.1 562,771 - PFLU_RS02445 0.56 -0.0 562,797 + PFLU_RS02445 0.60 +0.8 562,802 - PFLU_RS02445 0.61 -2.8 562,824 + PFLU_RS02445 0.64 -1.9 562,843 + PFLU_RS02445 0.67 -1.7 562,843 + PFLU_RS02445 0.67 -0.7 562,843 + PFLU_RS02445 0.67 -0.2 562,844 - PFLU_RS02445 0.68 +0.5 562,844 - PFLU_RS02445 0.68 +0.0 562,844 - PFLU_RS02445 0.68 -1.0 563,041 + +1.4 563,042 - -0.4 563,103 + PFLU_RS02450 0.15 -0.1 563,103 + PFLU_RS02450 0.15 -0.3 563,104 - PFLU_RS02450 0.16 +1.1 563,104 - PFLU_RS02450 0.16 +1.1 563,104 - PFLU_RS02450 0.16 -1.6 563,104 - PFLU_RS02450 0.16 -3.2 563,104 - PFLU_RS02450 0.16 -0.2 563,104 - PFLU_RS02450 0.16 -0.0 563,104 - PFLU_RS02450 0.16 -0.7 563,104 - PFLU_RS02450 0.16 +1.4 563,226 + PFLU_RS02450 0.43 -1.5 563,226 + PFLU_RS02450 0.43 +0.5 563,227 - PFLU_RS02450 0.43 +0.7 563,227 - PFLU_RS02450 0.43 +0.6 563,293 + PFLU_RS02450 0.58 +0.3 563,293 + PFLU_RS02450 0.58 +1.0 563,293 + PFLU_RS02450 0.58 -1.4 563,293 + PFLU_RS02450 0.58 -0.7 563,293 + PFLU_RS02450 0.58 +2.1 563,293 + PFLU_RS02450 0.58 -1.3 563,293 + PFLU_RS02450 0.58 +1.7 563,293 + PFLU_RS02450 0.58 +0.9 563,293 + PFLU_RS02450 0.58 -0.0 563,293 + PFLU_RS02450 0.58 +0.5 563,293 + PFLU_RS02450 0.58 +0.9 563,294 - PFLU_RS02450 0.58 +0.5 563,294 - PFLU_RS02450 0.58 -0.7 563,294 - PFLU_RS02450 0.58 -0.9 563,301 + PFLU_RS02450 0.59 +0.2 563,301 + PFLU_RS02450 0.59 +1.5 563,301 + PFLU_RS02450 0.59 -0.9 563,302 - PFLU_RS02450 0.60 +1.9 563,302 - PFLU_RS02450 0.60 +0.3 563,302 - PFLU_RS02450 0.60 +1.5 563,302 - PFLU_RS02450 0.60 +1.3 563,364 + PFLU_RS02450 0.73 -1.0 563,364 + PFLU_RS02450 0.73 -0.2 563,364 + PFLU_RS02450 0.73 +0.4 563,364 + PFLU_RS02450 0.73 +0.8 563,364 + PFLU_RS02450 0.73 +0.7 563,364 + PFLU_RS02450 0.73 +0.1 563,364 + PFLU_RS02450 0.73 -1.0 563,364 + PFLU_RS02450 0.73 +1.1 563,365 - PFLU_RS02450 0.74 +0.8 563,365 - PFLU_RS02450 0.74 -0.1 563,365 - PFLU_RS02450 0.74 +1.0 563,365 - PFLU_RS02450 0.74 +0.5 563,365 - PFLU_RS02450 0.74 +0.3 563,365 - PFLU_RS02450 0.74 -0.7 563,365 - PFLU_RS02450 0.74 +0.2 563,365 - PFLU_RS02450 0.74 -1.5 563,365 - PFLU_RS02450 0.74 -0.9 563,376 - PFLU_RS02450 0.76 +0.6 563,481 + -1.0 563,481 + -1.2 563,481 + -0.4 563,481 + -0.1 563,482 - -0.5 563,535 + PFLU_RS31380 0.66 +0.8 563,535 + PFLU_RS31380 0.66 +0.4 563,535 + PFLU_RS31380 0.66 +0.0 563,535 + PFLU_RS31380 0.66 +0.1 563,535 + PFLU_RS31380 0.66 +0.8 563,536 - PFLU_RS31380 0.66 -0.4 563,536 - PFLU_RS31380 0.66 +1.0 563,536 - PFLU_RS31380 0.66 -0.2 563,556 + PFLU_RS31380 0.77 +0.4 563,556 + PFLU_RS31380 0.77 -0.8 563,557 - PFLU_RS31380 0.77 +1.3 563,592 + +1.3 563,592 + -1.9 563,593 - -0.2 563,593 - +0.1 563,795 + PFLU_RS02455 0.26 -1.2 563,796 - PFLU_RS02455 0.26 -1.2 563,796 - PFLU_RS02455 0.26 +0.7 564,165 + PFLU_RS02455 0.71 -2.1 564,166 - PFLU_RS02455 0.72 -0.6 564,377 + +0.0 564,378 - +2.0 564,395 + -1.0 564,396 - +0.9 564,396 - -0.5 564,396 - -1.2 564,462 + +1.2 564,462 + +0.2 564,462 + +1.3 564,462 + +0.9 564,462 + -0.9 564,462 + +0.4 564,462 + -0.9 564,462 + +0.5 564,463 - +1.2 564,463 - +1.0 564,463 - +1.2 564,463 - +0.9 564,463 - +0.2 564,463 - -0.6 564,463 - +0.7 564,463 - +0.6 564,463 - +0.3 564,463 - -0.7 564,463 - -1.2 564,466 + -1.7 564,466 + -0.5 564,466 + -0.3 564,466 + +0.3 564,466 + +0.0 564,466 + +0.7 564,466 + -0.8 564,466 + +1.1 564,466 + +0.9 564,466 + +0.1 564,466 + +1.6 564,467 - +1.1 564,467 - +0.6 564,467 - +0.3 564,467 - +0.6 564,467 - +1.0 564,467 - +0.2 564,467 - -0.9 564,467 - +0.5 564,467 - -0.8 564,467 - +0.3 564,467 - -1.2 564,467 - +0.5 564,467 - +0.4 564,467 - -0.9 564,467 - -0.0 564,467 - -0.4 564,467 - -0.7 564,467 - +0.7 564,525 + +0.7 564,525 + +1.2 564,526 - +0.8 564,526 - +0.3 564,565 + -0.4 564,566 - -1.3 564,622 + -1.0 564,622 + +0.2 564,622 + -1.5 564,622 + -0.6 564,622 + -0.6 564,622 + +0.2 564,622 + -1.9 564,622 + -0.2 564,622 + -0.7 564,623 - -0.7 564,623 - -3.1 564,623 - -3.6 564,623 - -1.0 564,623 - -0.9 564,623 - -1.2 564,623 - -1.7 564,623 - -0.9 564,624 + +0.6 564,794 + PFLU_RS02460 0.31 -2.1 564,794 + PFLU_RS02460 0.31 -0.7 564,795 - PFLU_RS02460 0.31 -1.8 564,795 - PFLU_RS02460 0.31 -0.4 564,818 - PFLU_RS02460 0.35 -0.1 564,820 + PFLU_RS02460 0.35 -2.0 564,820 + PFLU_RS02460 0.35 -0.6 564,820 + PFLU_RS02460 0.35 -0.7 564,820 + PFLU_RS02460 0.35 -0.8 564,820 + PFLU_RS02460 0.35 -0.7 564,820 + PFLU_RS02460 0.35 -0.7 564,820 + PFLU_RS02460 0.35 -0.3 564,820 + PFLU_RS02460 0.35 -2.0 564,820 + PFLU_RS02460 0.35 -2.0 564,820 + PFLU_RS02460 0.35 -1.1 564,821 - PFLU_RS02460 0.36 -3.1 564,821 - PFLU_RS02460 0.36 -3.2 564,821 - PFLU_RS02460 0.36 -0.5 564,821 - PFLU_RS02460 0.36 -0.6 564,821 - PFLU_RS02460 0.36 -0.3 564,821 - PFLU_RS02460 0.36 -0.3 564,821 - PFLU_RS02460 0.36 -0.6 564,821 - PFLU_RS02460 0.36 -0.3 564,821 - PFLU_RS02460 0.36 -1.0 564,821 - PFLU_RS02460 0.36 -1.2 564,821 - PFLU_RS02460 0.36 -3.6 564,821 - PFLU_RS02460 0.36 -1.0 564,821 - PFLU_RS02460 0.36 -1.4 564,821 - PFLU_RS02460 0.36 -2.0 565,054 + PFLU_RS02460 0.74 +0.1 565,072 + PFLU_RS02460 0.77 -0.9 565,072 + PFLU_RS02460 0.77 -0.5 565,072 + PFLU_RS02460 0.77 -1.5 565,072 + PFLU_RS02460 0.77 -0.4 565,073 - PFLU_RS02460 0.77 -0.5 565,120 + PFLU_RS02460 0.85 -0.4 565,143 - PFLU_RS02460 0.89 -1.4 565,210 + +0.1 565,210 + +1.3 565,210 + +1.4 565,211 - -1.9 565,211 - -0.8 565,211 - -1.9 565,211 - -0.7 565,227 + -0.7 565,231 + +0.0
Or see this region's nucleotide sequence