Strain Fitness in Pseudomonas fluorescens SBW25 around PFLU_RS02310

Experiment: succinate (C); with MOPS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS02300 and PFLU_RS02305 overlap by 1 nucleotidesPFLU_RS02305 and PFLU_RS02310 overlap by 4 nucleotidesPFLU_RS02310 and PFLU_RS02315 overlap by 1 nucleotides PFLU_RS02300: PFLU_RS02300 - lipopolysaccharide core heptose(I) kinase RfaP, at 526,461 to 527,267 _RS02300 PFLU_RS02305: PFLU_RS02305 - LPS biosynthesis protein, at 527,267 to 528,001 _RS02305 PFLU_RS02310: PFLU_RS02310 - LPS kinase, at 527,998 to 528,750 _RS02310 PFLU_RS02315: PFLU_RS02315 - serine/threonine protein kinase, at 528,750 to 530,201 _RS02315 Position (kb) 527 528 529Strain fitness (log2 ratio) -1 0 1 2at 528.160 kb on + strand, within PFLU_RS02310at 528.160 kb on + strand, within PFLU_RS02310at 528.163 kb on + strand, within PFLU_RS02310at 528.163 kb on + strand, within PFLU_RS02310at 528.163 kb on + strand, within PFLU_RS02310at 528.184 kb on + strand, within PFLU_RS02310at 528.184 kb on + strand, within PFLU_RS02310at 528.184 kb on + strand, within PFLU_RS02310at 528.247 kb on - strand, within PFLU_RS02310at 528.498 kb on + strand, within PFLU_RS02310at 528.498 kb on + strand, within PFLU_RS02310at 528.498 kb on + strand, within PFLU_RS02310at 528.498 kb on + strand, within PFLU_RS02310at 528.664 kb on + strand, within PFLU_RS02310at 528.730 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction succinate (C); with MOPS
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528,160 + PFLU_RS02310 0.22 +0.6
528,160 + PFLU_RS02310 0.22 +0.0
528,163 + PFLU_RS02310 0.22 +0.1
528,163 + PFLU_RS02310 0.22 -1.2
528,163 + PFLU_RS02310 0.22 -1.0
528,184 + PFLU_RS02310 0.25 +1.1
528,184 + PFLU_RS02310 0.25 -0.4
528,184 + PFLU_RS02310 0.25 +0.2
528,247 - PFLU_RS02310 0.33 +2.3
528,498 + PFLU_RS02310 0.66 -1.3
528,498 + PFLU_RS02310 0.66 +0.0
528,498 + PFLU_RS02310 0.66 -0.4
528,498 + PFLU_RS02310 0.66 -0.2
528,664 + PFLU_RS02310 0.88 -0.2
528,730 - -0.6

Or see this region's nucleotide sequence