Experiment: succinate (C); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS01950 and PFLU_RS01955 are separated by 249 nucleotides PFLU_RS01955 and PFLU_RS01960 are separated by 148 nucleotides PFLU_RS01960 and PFLU_RS01965 are separated by 37 nucleotides PFLU_RS01965 and PFLU_RS01970 are separated by 257 nucleotides
PFLU_RS01950: PFLU_RS01950 - class II poly(R)-hydroxyalkanoic acid synthase, at 436,299 to 437,978
_RS01950
PFLU_RS01955: PFLU_RS01955 - DUF971 domain-containing protein, at 438,228 to 438,605
_RS01955
PFLU_RS01960: PFLU_RS01960 - ATP-dependent protease ATPase subunit HslU, at 438,754 to 440,091
_RS01960
PFLU_RS01965: PFLU_RS01965 - ATP-dependent protease subunit HslV, at 440,129 to 440,659
_RS01965
PFLU_RS01970: PFLU_RS01970 - cell division protein, at 440,917 to 441,618
_RS01970
Position (kb) 438
439
440
441 Strain fitness (log2 ratio) -2
-1
0
1
2 at 437.886 kb on + strand at 437.886 kb on + strand at 437.887 kb on - strand at 437.972 kb on + strand at 437.980 kb on + strand at 437.980 kb on + strand at 437.980 kb on + strand at 437.980 kb on + strand at 437.980 kb on + strand at 437.981 kb on - strand at 437.981 kb on - strand at 437.981 kb on - strand at 438.026 kb on - strand at 438.194 kb on + strand at 438.208 kb on + strand at 438.208 kb on + strand at 438.209 kb on - strand at 438.229 kb on + strand at 438.298 kb on + strand, within PFLU_RS01955 at 438.299 kb on - strand, within PFLU_RS01955 at 438.299 kb on - strand, within PFLU_RS01955 at 438.337 kb on + strand, within PFLU_RS01955 at 438.337 kb on + strand, within PFLU_RS01955 at 438.338 kb on - strand, within PFLU_RS01955 at 438.343 kb on + strand, within PFLU_RS01955 at 438.343 kb on + strand, within PFLU_RS01955 at 438.343 kb on + strand, within PFLU_RS01955 at 438.343 kb on + strand, within PFLU_RS01955 at 438.343 kb on + strand, within PFLU_RS01955 at 438.343 kb on + strand, within PFLU_RS01955 at 438.344 kb on - strand, within PFLU_RS01955 at 438.344 kb on - strand, within PFLU_RS01955 at 438.400 kb on + strand, within PFLU_RS01955 at 438.400 kb on + strand, within PFLU_RS01955 at 438.400 kb on + strand, within PFLU_RS01955 at 438.401 kb on - strand, within PFLU_RS01955 at 438.464 kb on + strand, within PFLU_RS01955 at 438.526 kb on + strand, within PFLU_RS01955 at 438.526 kb on + strand, within PFLU_RS01955 at 438.526 kb on + strand, within PFLU_RS01955 at 438.527 kb on - strand, within PFLU_RS01955 at 438.527 kb on - strand, within PFLU_RS01955 at 438.527 kb on - strand, within PFLU_RS01955 at 438.527 kb on - strand, within PFLU_RS01955 at 438.545 kb on - strand, within PFLU_RS01955 at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.605 kb on + strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.606 kb on - strand at 438.609 kb on + strand at 438.609 kb on + strand at 438.610 kb on - strand at 438.610 kb on - strand at 438.610 kb on - strand at 438.610 kb on - strand at 438.662 kb on - strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.681 kb on + strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.682 kb on - strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.683 kb on + strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.684 kb on - strand at 438.755 kb on + strand at 438.816 kb on - strand at 439.109 kb on - strand, within PFLU_RS01960 at 439.109 kb on - strand, within PFLU_RS01960 at 439.823 kb on + strand, within PFLU_RS01960 at 439.824 kb on - strand, within PFLU_RS01960 at 440.047 kb on - strand at 440.047 kb on - strand at 440.091 kb on + strand at 440.091 kb on + strand at 440.091 kb on + strand at 440.091 kb on + strand at 440.091 kb on + strand at 440.091 kb on + strand at 440.091 kb on + strand at 440.091 kb on + strand at 440.091 kb on + strand at 440.091 kb on + strand at 440.091 kb on + strand at 440.091 kb on + strand at 440.091 kb on + strand at 440.092 kb on - strand at 440.092 kb on - strand at 440.092 kb on - strand at 440.092 kb on - strand at 440.092 kb on - strand at 440.092 kb on - strand at 440.092 kb on - strand at 440.092 kb on - strand at 440.092 kb on - strand at 440.117 kb on + strand at 440.118 kb on - strand at 440.185 kb on + strand, within PFLU_RS01965 at 440.185 kb on + strand, within PFLU_RS01965 at 440.186 kb on - strand, within PFLU_RS01965 at 440.545 kb on - strand, within PFLU_RS01965 at 440.585 kb on - strand, within PFLU_RS01965 at 440.585 kb on - strand, within PFLU_RS01965 at 440.585 kb on - strand, within PFLU_RS01965 at 440.585 kb on - strand, within PFLU_RS01965 at 440.588 kb on - strand, within PFLU_RS01965 at 440.640 kb on - strand at 440.640 kb on - strand at 440.738 kb on - strand at 440.864 kb on + strand at 440.864 kb on + strand at 440.864 kb on + strand at 440.864 kb on + strand at 440.864 kb on + strand at 440.864 kb on + strand at 440.864 kb on + strand at 440.864 kb on + strand at 440.864 kb on + strand at 440.864 kb on + strand at 440.864 kb on + strand at 440.864 kb on + strand at 440.865 kb on - strand at 440.865 kb on - strand at 440.865 kb on - strand at 440.865 kb on - strand at 440.865 kb on - strand at 440.865 kb on - strand at 440.939 kb on - strand at 440.939 kb on - strand at 440.939 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction succinate (C); with MOPS remove 437,886 + -0.5 437,886 + +0.4 437,887 - +1.7 437,972 + +1.0 437,980 + -1.5 437,980 + -2.6 437,980 + +0.1 437,980 + +1.6 437,980 + +1.1 437,981 - -0.8 437,981 - +0.7 437,981 - -0.5 438,026 - -0.8 438,194 + -0.5 438,208 + -0.2 438,208 + -0.9 438,209 - +1.1 438,229 + +1.4 438,298 + PFLU_RS01955 0.19 -0.7 438,299 - PFLU_RS01955 0.19 +0.7 438,299 - PFLU_RS01955 0.19 +0.0 438,337 + PFLU_RS01955 0.29 +0.5 438,337 + PFLU_RS01955 0.29 -0.6 438,338 - PFLU_RS01955 0.29 +1.4 438,343 + PFLU_RS01955 0.30 +0.2 438,343 + PFLU_RS01955 0.30 -1.3 438,343 + PFLU_RS01955 0.30 +1.0 438,343 + PFLU_RS01955 0.30 -0.1 438,343 + PFLU_RS01955 0.30 +1.3 438,343 + PFLU_RS01955 0.30 +0.9 438,344 - PFLU_RS01955 0.31 +0.2 438,344 - PFLU_RS01955 0.31 -0.1 438,400 + PFLU_RS01955 0.46 +0.2 438,400 + PFLU_RS01955 0.46 -0.4 438,400 + PFLU_RS01955 0.46 +1.9 438,401 - PFLU_RS01955 0.46 -2.4 438,464 + PFLU_RS01955 0.62 +1.7 438,526 + PFLU_RS01955 0.79 -1.4 438,526 + PFLU_RS01955 0.79 -1.3 438,526 + PFLU_RS01955 0.79 -0.5 438,527 - PFLU_RS01955 0.79 +1.7 438,527 - PFLU_RS01955 0.79 +0.5 438,527 - PFLU_RS01955 0.79 -1.0 438,527 - PFLU_RS01955 0.79 -1.1 438,545 - PFLU_RS01955 0.84 +0.7 438,605 + +0.7 438,605 + -1.4 438,605 + -0.1 438,605 + -0.0 438,605 + +0.4 438,605 + +0.4 438,605 + +1.6 438,605 + -0.7 438,605 + +0.5 438,605 + -0.3 438,605 + -0.7 438,605 + +0.7 438,605 + +0.7 438,605 + -0.1 438,605 + -0.1 438,605 + -0.2 438,605 + +0.6 438,605 + +0.5 438,605 + -0.0 438,605 + -1.1 438,606 - +0.5 438,606 - -0.3 438,606 - +0.6 438,606 - +0.7 438,606 - +1.3 438,606 - +0.8 438,606 - +0.3 438,606 - +0.9 438,606 - -1.3 438,606 - +0.3 438,606 - -1.6 438,606 - +0.7 438,606 - +0.1 438,606 - -0.4 438,606 - -0.4 438,606 - -0.9 438,606 - -0.0 438,606 - +0.5 438,606 - +0.3 438,606 - -0.5 438,606 - -0.6 438,606 - -0.8 438,606 - +0.8 438,606 - +0.6 438,606 - -0.7 438,609 + +0.5 438,609 + +0.2 438,610 - +1.2 438,610 - +0.3 438,610 - +2.3 438,610 - -0.0 438,662 - -0.5 438,681 + -0.2 438,681 + -0.2 438,681 + +1.4 438,681 + -0.0 438,681 + +1.1 438,681 + -2.0 438,681 + -0.1 438,681 + -0.5 438,681 + -0.4 438,681 + -0.4 438,681 + +0.6 438,681 + -0.3 438,681 + -0.5 438,681 + +0.7 438,681 + +0.9 438,681 + +1.5 438,681 + -0.8 438,681 + -1.1 438,681 + -0.0 438,681 + +0.7 438,681 + -0.5 438,681 + -0.1 438,682 - -0.3 438,682 - +0.7 438,682 - +0.2 438,682 - -0.2 438,682 - -0.8 438,682 - +0.6 438,682 - +0.7 438,682 - -0.6 438,682 - +0.3 438,682 - +0.1 438,682 - +0.6 438,682 - -1.0 438,682 - -0.1 438,682 - -1.3 438,682 - +1.0 438,682 - +0.6 438,682 - +0.7 438,682 - -0.7 438,682 - +0.7 438,682 - +1.7 438,682 - +0.5 438,682 - +0.9 438,682 - -0.4 438,682 - +1.4 438,682 - -1.1 438,682 - -1.8 438,682 - +0.7 438,682 - -0.2 438,682 - +0.7 438,682 - +0.4 438,682 - -0.4 438,683 + +0.6 438,683 + +0.6 438,683 + -0.1 438,683 + -0.2 438,683 + -0.2 438,683 + -0.8 438,683 + +0.3 438,683 + -0.8 438,683 + -0.6 438,683 + +0.9 438,683 + +0.2 438,683 + -1.5 438,683 + -0.2 438,683 + -0.5 438,683 + +0.0 438,683 + -0.9 438,683 + -1.0 438,683 + +0.1 438,683 + +0.7 438,683 + +0.5 438,683 + +1.5 438,683 + -1.2 438,683 + -0.1 438,683 + +0.6 438,683 + -0.3 438,684 - +0.9 438,684 - +0.7 438,684 - +0.6 438,684 - -0.4 438,684 - -0.9 438,684 - +0.7 438,684 - +0.5 438,684 - +0.2 438,684 - +0.7 438,684 - -0.2 438,684 - +0.6 438,684 - -1.1 438,684 - +0.4 438,684 - +0.7 438,684 - -0.2 438,684 - -0.1 438,684 - -0.3 438,684 - +1.5 438,684 - -1.4 438,684 - +0.7 438,684 - +0.2 438,684 - -0.2 438,684 - +0.0 438,755 + +0.3 438,816 - +2.3 439,109 - PFLU_RS01960 0.27 +1.3 439,109 - PFLU_RS01960 0.27 -0.5 439,823 + PFLU_RS01960 0.80 +0.7 439,824 - PFLU_RS01960 0.80 +2.3 440,047 - +0.3 440,047 - +0.0 440,091 + -0.8 440,091 + +0.3 440,091 + +0.3 440,091 + -1.0 440,091 + -1.1 440,091 + +1.6 440,091 + +1.3 440,091 + +1.3 440,091 + +0.7 440,091 + -0.3 440,091 + -0.3 440,091 + +1.7 440,091 + +0.7 440,092 - -0.7 440,092 - +2.3 440,092 - +2.3 440,092 - +0.3 440,092 - -0.3 440,092 - -0.5 440,092 - -0.3 440,092 - +0.3 440,092 - +2.3 440,117 + +1.0 440,118 - +0.3 440,185 + PFLU_RS01965 0.11 +0.3 440,185 + PFLU_RS01965 0.11 +1.3 440,186 - PFLU_RS01965 0.11 +1.3 440,545 - PFLU_RS01965 0.78 -0.3 440,585 - PFLU_RS01965 0.86 +2.3 440,585 - PFLU_RS01965 0.86 +0.5 440,585 - PFLU_RS01965 0.86 +1.7 440,585 - PFLU_RS01965 0.86 +1.1 440,588 - PFLU_RS01965 0.86 +0.7 440,640 - -1.2 440,640 - -1.0 440,738 - +0.3 440,864 + -0.5 440,864 + -0.1 440,864 + -0.0 440,864 + -0.1 440,864 + -0.9 440,864 + +0.9 440,864 + -1.0 440,864 + -0.3 440,864 + +0.1 440,864 + -1.6 440,864 + -0.3 440,864 + -0.3 440,865 - +0.2 440,865 - +0.5 440,865 - -1.8 440,865 - +0.0 440,865 - +0.0 440,865 - +0.0 440,939 - +0.0 440,939 - -0.1 440,939 - +1.2
Or see this region's nucleotide sequence