Experiment: succinate (C); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS01615 and PFLU_RS01620 are separated by 39 nucleotides PFLU_RS01620 and PFLU_RS01625 are separated by 9 nucleotides PFLU_RS01625 and PFLU_RS01630 are separated by 66 nucleotides PFLU_RS01630 and PFLU_RS01635 are separated by 3 nucleotides
PFLU_RS01615: PFLU_RS01615 - DUF2164 domain-containing protein, at 361,872 to 362,129
_RS01615
PFLU_RS01620: PFLU_RS01620 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 362,169 to 362,906
_RS01620
PFLU_RS01625: PFLU_RS01625 - imidazole glycerol phosphate synthase subunit HisF, at 362,916 to 363,686
_RS01625
PFLU_RS01630: PFLU_RS01630 - ABC transporter substrate-binding protein, at 363,753 to 364,508
_RS01630
PFLU_RS01635: PFLU_RS01635 - serine protease, at 364,512 to 366,077
_RS01635
Position (kb) 362
363
364 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 361.931 kb on + strand, within PFLU_RS01615 at 362.037 kb on + strand, within PFLU_RS01615 at 362.037 kb on + strand, within PFLU_RS01615 at 362.038 kb on - strand, within PFLU_RS01615 at 362.040 kb on + strand, within PFLU_RS01615 at 362.040 kb on + strand, within PFLU_RS01615 at 362.041 kb on - strand, within PFLU_RS01615 at 362.041 kb on - strand, within PFLU_RS01615 at 362.041 kb on - strand, within PFLU_RS01615 at 362.156 kb on + strand at 362.177 kb on + strand at 362.178 kb on - strand at 362.178 kb on - strand at 362.212 kb on + strand at 362.212 kb on + strand at 362.212 kb on + strand at 362.213 kb on - strand at 362.213 kb on - strand at 362.213 kb on - strand at 362.213 kb on - strand at 362.213 kb on - strand at 362.213 kb on - strand at 362.213 kb on - strand at 362.445 kb on + strand, within PFLU_RS01620 at 362.445 kb on + strand, within PFLU_RS01620 at 362.445 kb on + strand, within PFLU_RS01620 at 362.446 kb on - strand, within PFLU_RS01620 at 362.446 kb on - strand, within PFLU_RS01620 at 362.446 kb on - strand, within PFLU_RS01620 at 362.446 kb on - strand, within PFLU_RS01620 at 362.446 kb on - strand, within PFLU_RS01620 at 362.449 kb on + strand, within PFLU_RS01620 at 362.449 kb on + strand, within PFLU_RS01620 at 362.449 kb on + strand, within PFLU_RS01620 at 362.449 kb on + strand, within PFLU_RS01620 at 362.449 kb on + strand, within PFLU_RS01620 at 362.449 kb on + strand, within PFLU_RS01620 at 362.449 kb on + strand, within PFLU_RS01620 at 362.449 kb on + strand, within PFLU_RS01620 at 362.449 kb on + strand, within PFLU_RS01620 at 362.449 kb on + strand, within PFLU_RS01620 at 362.449 kb on + strand, within PFLU_RS01620 at 362.449 kb on + strand, within PFLU_RS01620 at 362.449 kb on + strand, within PFLU_RS01620 at 362.449 kb on + strand, within PFLU_RS01620 at 362.450 kb on - strand, within PFLU_RS01620 at 362.450 kb on - strand, within PFLU_RS01620 at 362.450 kb on - strand, within PFLU_RS01620 at 362.450 kb on - strand, within PFLU_RS01620 at 362.450 kb on - strand, within PFLU_RS01620 at 362.466 kb on + strand, within PFLU_RS01620 at 362.466 kb on + strand, within PFLU_RS01620 at 362.466 kb on + strand, within PFLU_RS01620 at 362.466 kb on + strand, within PFLU_RS01620 at 362.467 kb on - strand, within PFLU_RS01620 at 362.467 kb on - strand, within PFLU_RS01620 at 362.467 kb on - strand, within PFLU_RS01620 at 362.467 kb on - strand, within PFLU_RS01620 at 362.470 kb on + strand, within PFLU_RS01620 at 362.470 kb on + strand, within PFLU_RS01620 at 362.470 kb on + strand, within PFLU_RS01620 at 362.471 kb on - strand, within PFLU_RS01620 at 362.471 kb on - strand, within PFLU_RS01620 at 362.471 kb on - strand, within PFLU_RS01620 at 362.471 kb on - strand, within PFLU_RS01620 at 362.471 kb on - strand, within PFLU_RS01620 at 362.665 kb on - strand, within PFLU_RS01620 at 362.665 kb on - strand, within PFLU_RS01620 at 362.847 kb on + strand at 362.847 kb on + strand at 362.847 kb on + strand at 362.847 kb on + strand at 362.848 kb on - strand at 362.855 kb on + strand at 362.856 kb on - strand at 362.883 kb on + strand at 362.883 kb on + strand at 362.884 kb on - strand at 362.895 kb on + strand at 362.895 kb on + strand at 363.060 kb on - strand, within PFLU_RS01625 at 363.060 kb on - strand, within PFLU_RS01625 at 363.110 kb on + strand, within PFLU_RS01625 at 363.612 kb on - strand at 363.631 kb on - strand at 363.631 kb on - strand at 363.631 kb on - strand at 363.654 kb on + strand at 363.654 kb on + strand at 363.655 kb on - strand at 363.655 kb on - strand at 363.655 kb on - strand at 363.655 kb on - strand at 363.655 kb on - strand at 363.655 kb on - strand at 363.785 kb on + strand at 363.786 kb on - strand at 363.786 kb on - strand at 363.786 kb on - strand at 363.870 kb on + strand, within PFLU_RS01630 at 363.870 kb on + strand, within PFLU_RS01630 at 363.972 kb on + strand, within PFLU_RS01630 at 363.972 kb on + strand, within PFLU_RS01630 at 364.008 kb on + strand, within PFLU_RS01630 at 364.009 kb on - strand, within PFLU_RS01630 at 364.009 kb on - strand, within PFLU_RS01630 at 364.036 kb on - strand, within PFLU_RS01630 at 364.137 kb on - strand, within PFLU_RS01630 at 364.137 kb on - strand, within PFLU_RS01630 at 364.137 kb on - strand, within PFLU_RS01630 at 364.137 kb on - strand, within PFLU_RS01630 at 364.176 kb on + strand, within PFLU_RS01630 at 364.191 kb on + strand, within PFLU_RS01630 at 364.193 kb on + strand, within PFLU_RS01630 at 364.194 kb on - strand, within PFLU_RS01630 at 364.235 kb on + strand, within PFLU_RS01630 at 364.236 kb on - strand, within PFLU_RS01630 at 364.380 kb on - strand, within PFLU_RS01630 at 364.380 kb on - strand, within PFLU_RS01630 at 364.396 kb on - strand, within PFLU_RS01630 at 364.500 kb on + strand at 364.500 kb on + strand at 364.501 kb on - strand at 364.501 kb on - strand at 364.501 kb on - strand at 364.506 kb on + strand at 364.507 kb on - strand at 364.573 kb on + strand at 364.573 kb on + strand at 364.573 kb on + strand at 364.573 kb on + strand at 364.574 kb on - strand at 364.574 kb on - strand at 364.574 kb on - strand at 364.610 kb on + strand at 364.611 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction succinate (C); with MOPS remove 361,931 + PFLU_RS01615 0.23 +0.6 362,037 + PFLU_RS01615 0.64 +0.0 362,037 + PFLU_RS01615 0.64 +0.0 362,038 - PFLU_RS01615 0.64 -2.3 362,040 + PFLU_RS01615 0.65 +0.2 362,040 + PFLU_RS01615 0.65 -0.4 362,041 - PFLU_RS01615 0.66 -2.3 362,041 - PFLU_RS01615 0.66 -2.7 362,041 - PFLU_RS01615 0.66 -2.0 362,156 + -2.5 362,177 + -2.1 362,178 - -1.6 362,178 - -3.2 362,212 + -1.0 362,212 + -2.6 362,212 + -3.0 362,213 - -3.1 362,213 - -3.1 362,213 - -3.2 362,213 - -3.3 362,213 - -0.7 362,213 - -1.8 362,213 - -2.4 362,445 + PFLU_RS01620 0.37 -2.6 362,445 + PFLU_RS01620 0.37 -1.7 362,445 + PFLU_RS01620 0.37 -1.9 362,446 - PFLU_RS01620 0.38 -2.8 362,446 - PFLU_RS01620 0.38 -3.4 362,446 - PFLU_RS01620 0.38 -2.4 362,446 - PFLU_RS01620 0.38 -2.0 362,446 - PFLU_RS01620 0.38 -0.5 362,449 + PFLU_RS01620 0.38 -3.5 362,449 + PFLU_RS01620 0.38 -3.5 362,449 + PFLU_RS01620 0.38 -1.7 362,449 + PFLU_RS01620 0.38 -3.2 362,449 + PFLU_RS01620 0.38 -2.8 362,449 + PFLU_RS01620 0.38 -1.9 362,449 + PFLU_RS01620 0.38 -0.8 362,449 + PFLU_RS01620 0.38 -3.2 362,449 + PFLU_RS01620 0.38 -3.0 362,449 + PFLU_RS01620 0.38 -3.0 362,449 + PFLU_RS01620 0.38 -2.8 362,449 + PFLU_RS01620 0.38 -1.5 362,449 + PFLU_RS01620 0.38 -3.7 362,449 + PFLU_RS01620 0.38 -3.2 362,450 - PFLU_RS01620 0.38 -1.8 362,450 - PFLU_RS01620 0.38 -1.0 362,450 - PFLU_RS01620 0.38 -2.5 362,450 - PFLU_RS01620 0.38 -2.2 362,450 - PFLU_RS01620 0.38 -3.2 362,466 + PFLU_RS01620 0.40 -2.4 362,466 + PFLU_RS01620 0.40 -3.6 362,466 + PFLU_RS01620 0.40 -3.1 362,466 + PFLU_RS01620 0.40 -2.8 362,467 - PFLU_RS01620 0.40 -2.5 362,467 - PFLU_RS01620 0.40 +0.0 362,467 - PFLU_RS01620 0.40 -2.0 362,467 - PFLU_RS01620 0.40 -2.7 362,470 + PFLU_RS01620 0.41 -2.8 362,470 + PFLU_RS01620 0.41 -3.6 362,470 + PFLU_RS01620 0.41 -1.8 362,471 - PFLU_RS01620 0.41 -0.5 362,471 - PFLU_RS01620 0.41 -2.8 362,471 - PFLU_RS01620 0.41 -3.2 362,471 - PFLU_RS01620 0.41 -2.2 362,471 - PFLU_RS01620 0.41 -0.8 362,665 - PFLU_RS01620 0.67 -0.2 362,665 - PFLU_RS01620 0.67 -2.6 362,847 + -3.1 362,847 + -2.7 362,847 + -3.5 362,847 + -0.7 362,848 - -2.9 362,855 + -2.2 362,856 - -3.3 362,883 + -1.4 362,883 + -0.2 362,884 - -2.8 362,895 + -3.1 362,895 + +0.3 363,060 - PFLU_RS01625 0.19 -2.2 363,060 - PFLU_RS01625 0.19 -1.9 363,110 + PFLU_RS01625 0.25 -1.8 363,612 - -1.7 363,631 - -0.8 363,631 - -1.2 363,631 - -1.8 363,654 + -1.7 363,654 + -3.0 363,655 - -2.4 363,655 - -0.5 363,655 - -1.7 363,655 - -1.0 363,655 - -0.5 363,655 - +1.3 363,785 + -0.9 363,786 - -0.1 363,786 - -0.5 363,786 - +0.0 363,870 + PFLU_RS01630 0.15 -1.4 363,870 + PFLU_RS01630 0.15 +0.2 363,972 + PFLU_RS01630 0.29 -0.2 363,972 + PFLU_RS01630 0.29 +1.1 364,008 + PFLU_RS01630 0.34 +0.6 364,009 - PFLU_RS01630 0.34 +0.3 364,009 - PFLU_RS01630 0.34 -0.2 364,036 - PFLU_RS01630 0.37 +0.3 364,137 - PFLU_RS01630 0.51 +0.6 364,137 - PFLU_RS01630 0.51 -0.3 364,137 - PFLU_RS01630 0.51 +0.6 364,137 - PFLU_RS01630 0.51 -0.1 364,176 + PFLU_RS01630 0.56 +0.0 364,191 + PFLU_RS01630 0.58 -0.1 364,193 + PFLU_RS01630 0.58 +0.9 364,194 - PFLU_RS01630 0.58 +0.3 364,235 + PFLU_RS01630 0.64 -1.4 364,236 - PFLU_RS01630 0.64 +0.3 364,380 - PFLU_RS01630 0.83 +0.9 364,380 - PFLU_RS01630 0.83 -0.1 364,396 - PFLU_RS01630 0.85 -0.2 364,500 + -0.9 364,500 + -1.2 364,501 - +0.7 364,501 - -0.8 364,501 - -0.4 364,506 + +0.8 364,507 - +2.1 364,573 + -0.8 364,573 + +0.1 364,573 + +0.8 364,573 + -1.5 364,574 - -0.1 364,574 - +0.9 364,574 - +2.3 364,610 + +0.1 364,611 - -0.8
Or see this region's nucleotide sequence