Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2774 and EX28DRAFT_2775 are separated by 83 nucleotides EX28DRAFT_2775 and EX28DRAFT_2776 overlap by 4 nucleotides EX28DRAFT_2776 and EX28DRAFT_2777 are separated by 117 nucleotides EX28DRAFT_2777 and EX28DRAFT_2778 are separated by 1 nucleotides EX28DRAFT_2778 and EX28DRAFT_2779 are separated by 29 nucleotides
EX28DRAFT_2774: EX28DRAFT_2774 - glutamate-cysteine ligase (EC 6.3.2.2), at 81,896 to 83,440
_2774
EX28DRAFT_2775: EX28DRAFT_2775 - Predicted membrane protein, at 83,524 to 83,952
_2775
EX28DRAFT_2776: EX28DRAFT_2776 - haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED/beta-phosphoglucomutase family hydrolase, at 83,949 to 84,515
_2776
EX28DRAFT_2777: EX28DRAFT_2777 - RNA, at 84,633 to 84,764
_2777
EX28DRAFT_2778: EX28DRAFT_2778 - tRNA, at 84,766 to 84,842
_2778
EX28DRAFT_2779: EX28DRAFT_2779 - tRNA, at 84,872 to 84,948
_2779
Position (kb) 83
84 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 82.677 kb on + strand, within EX28DRAFT_2774 at 82.700 kb on - strand, within EX28DRAFT_2774 at 82.724 kb on - strand, within EX28DRAFT_2774 at 82.777 kb on - strand, within EX28DRAFT_2774 at 82.914 kb on + strand, within EX28DRAFT_2774 at 82.915 kb on - strand, within EX28DRAFT_2774 at 82.989 kb on + strand, within EX28DRAFT_2774 at 83.121 kb on + strand, within EX28DRAFT_2774 at 83.121 kb on + strand, within EX28DRAFT_2774 at 83.147 kb on - strand, within EX28DRAFT_2774 at 83.206 kb on + strand, within EX28DRAFT_2774 at 83.216 kb on - strand, within EX28DRAFT_2774 at 83.279 kb on - strand, within EX28DRAFT_2774 at 83.329 kb on + strand at 83.426 kb on + strand at 83.463 kb on + strand at 83.464 kb on - strand at 83.464 kb on - strand at 83.469 kb on + strand at 83.470 kb on - strand at 83.479 kb on - strand at 83.525 kb on + strand at 83.525 kb on + strand at 83.541 kb on + strand at 83.541 kb on + strand at 83.542 kb on - strand at 83.549 kb on - strand at 83.549 kb on - strand at 83.653 kb on + strand, within EX28DRAFT_2775 at 83.654 kb on - strand, within EX28DRAFT_2775 at 83.665 kb on + strand, within EX28DRAFT_2775 at 83.665 kb on + strand, within EX28DRAFT_2775 at 83.665 kb on + strand, within EX28DRAFT_2775 at 83.666 kb on - strand, within EX28DRAFT_2775 at 83.681 kb on - strand, within EX28DRAFT_2775 at 83.683 kb on + strand, within EX28DRAFT_2775 at 83.776 kb on - strand, within EX28DRAFT_2775 at 83.802 kb on + strand, within EX28DRAFT_2775 at 83.815 kb on + strand, within EX28DRAFT_2775 at 83.818 kb on + strand, within EX28DRAFT_2775 at 83.821 kb on + strand, within EX28DRAFT_2775 at 83.822 kb on - strand, within EX28DRAFT_2775 at 83.822 kb on - strand, within EX28DRAFT_2775 at 83.822 kb on - strand, within EX28DRAFT_2775 at 84.228 kb on + strand, within EX28DRAFT_2776 at 84.229 kb on - strand, within EX28DRAFT_2776 at 84.229 kb on - strand, within EX28DRAFT_2776 at 84.233 kb on + strand, within EX28DRAFT_2776 at 84.503 kb on - strand at 84.511 kb on + strand at 84.511 kb on + strand at 84.512 kb on - strand at 84.512 kb on - strand at 84.546 kb on + strand at 84.556 kb on + strand at 84.557 kb on - strand at 84.718 kb on + strand, within EX28DRAFT_2777 at 84.753 kb on - strand at 84.765 kb on - strand at 84.765 kb on - strand at 84.860 kb on - strand at 84.868 kb on + strand at 84.869 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 82,677 + EX28DRAFT_2774 0.51 -3.1 82,700 - EX28DRAFT_2774 0.52 -3.3 82,724 - EX28DRAFT_2774 0.54 -3.2 82,777 - EX28DRAFT_2774 0.57 -4.1 82,914 + EX28DRAFT_2774 0.66 -4.0 82,915 - EX28DRAFT_2774 0.66 +0.1 82,989 + EX28DRAFT_2774 0.71 -4.3 83,121 + EX28DRAFT_2774 0.79 -2.9 83,121 + EX28DRAFT_2774 0.79 -1.2 83,147 - EX28DRAFT_2774 0.81 -3.3 83,206 + EX28DRAFT_2774 0.85 -3.5 83,216 - EX28DRAFT_2774 0.85 -0.2 83,279 - EX28DRAFT_2774 0.90 -2.2 83,329 + -3.8 83,426 + -3.5 83,463 + -3.4 83,464 - -0.6 83,464 - -1.7 83,469 + -4.7 83,470 - -1.1 83,479 - -0.8 83,525 + -4.3 83,525 + -4.1 83,541 + -4.2 83,541 + -3.0 83,542 - -0.1 83,549 - -0.3 83,549 - +0.3 83,653 + EX28DRAFT_2775 0.30 -3.3 83,654 - EX28DRAFT_2775 0.30 -0.5 83,665 + EX28DRAFT_2775 0.33 -1.1 83,665 + EX28DRAFT_2775 0.33 -2.1 83,665 + EX28DRAFT_2775 0.33 -2.8 83,666 - EX28DRAFT_2775 0.33 +0.1 83,681 - EX28DRAFT_2775 0.37 -1.0 83,683 + EX28DRAFT_2775 0.37 -1.7 83,776 - EX28DRAFT_2775 0.59 -0.7 83,802 + EX28DRAFT_2775 0.65 -1.5 83,815 + EX28DRAFT_2775 0.68 +0.4 83,818 + EX28DRAFT_2775 0.69 -0.1 83,821 + EX28DRAFT_2775 0.69 +0.7 83,822 - EX28DRAFT_2775 0.69 -0.4 83,822 - EX28DRAFT_2775 0.69 -0.7 83,822 - EX28DRAFT_2775 0.69 -0.7 84,228 + EX28DRAFT_2776 0.49 -0.3 84,229 - EX28DRAFT_2776 0.49 +0.5 84,229 - EX28DRAFT_2776 0.49 +0.7 84,233 + EX28DRAFT_2776 0.50 -0.1 84,503 - +1.2 84,511 + -0.4 84,511 + -0.3 84,512 - -1.4 84,512 - +0.3 84,546 + -1.1 84,556 + +0.5 84,557 - +0.1 84,718 + EX28DRAFT_2777 0.64 -0.2 84,753 - +0.9 84,765 - +0.3 84,765 - +0.1 84,860 - -0.3 84,868 + +0.6 84,869 - +0.1
Or see this region's nucleotide sequence