Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1912 and EX28DRAFT_1913 overlap by 4 nucleotides EX28DRAFT_1913 and EX28DRAFT_1914 are separated by 245 nucleotides EX28DRAFT_1914 and EX28DRAFT_1915 overlap by 1 nucleotides EX28DRAFT_1915 and EX28DRAFT_1916 are separated by 11 nucleotides
EX28DRAFT_1912: EX28DRAFT_1912 - Uncharacterized protein conserved in bacteria, at 1,924,225 to 1,924,584
_1912
EX28DRAFT_1913: EX28DRAFT_1913 - transcriptional regulator, TetR family, at 1,924,581 to 1,925,219
_1913
EX28DRAFT_1914: EX28DRAFT_1914 - pyrimidine utilization protein A, at 1,925,465 to 1,926,556
_1914
EX28DRAFT_1915: EX28DRAFT_1915 - pyrimidine utilization protein B, at 1,926,556 to 1,927,245
_1915
EX28DRAFT_1916: EX28DRAFT_1916 - pyrimidine utilization protein C, at 1,927,257 to 1,927,643
_1916
Position (kb)
1925
1926
1927 Strain fitness (log2 ratio)
-2
-1
0
1 at 1924.556 kb on + strand at 1924.664 kb on + strand, within EX28DRAFT_1913 at 1924.665 kb on - strand, within EX28DRAFT_1913 at 1924.703 kb on - strand, within EX28DRAFT_1913 at 1924.931 kb on - strand, within EX28DRAFT_1913 at 1925.023 kb on + strand, within EX28DRAFT_1913 at 1925.023 kb on + strand, within EX28DRAFT_1913 at 1925.024 kb on - strand, within EX28DRAFT_1913 at 1925.045 kb on - strand, within EX28DRAFT_1913 at 1925.047 kb on + strand, within EX28DRAFT_1913 at 1925.189 kb on + strand at 1925.384 kb on - strand at 1925.446 kb on + strand at 1925.446 kb on + strand at 1925.447 kb on - strand at 1925.478 kb on + strand at 1925.479 kb on - strand at 1925.531 kb on + strand at 1925.532 kb on - strand at 1925.554 kb on + strand at 1925.714 kb on + strand, within EX28DRAFT_1914 at 1925.715 kb on - strand, within EX28DRAFT_1914 at 1925.837 kb on + strand, within EX28DRAFT_1914 at 1925.904 kb on - strand, within EX28DRAFT_1914 at 1925.904 kb on - strand, within EX28DRAFT_1914 at 1926.176 kb on + strand, within EX28DRAFT_1914 at 1926.176 kb on + strand, within EX28DRAFT_1914 at 1926.239 kb on + strand, within EX28DRAFT_1914 at 1926.386 kb on + strand, within EX28DRAFT_1914 at 1927.090 kb on + strand, within EX28DRAFT_1915 at 1927.341 kb on + strand, within EX28DRAFT_1916 at 1927.341 kb on + strand, within EX28DRAFT_1916 at 1927.342 kb on - strand, within EX28DRAFT_1916 at 1927.342 kb on - strand, within EX28DRAFT_1916 at 1927.389 kb on - strand, within EX28DRAFT_1916 at 1927.512 kb on + strand, within EX28DRAFT_1916 at 1927.533 kb on + strand, within EX28DRAFT_1916 at 1927.533 kb on + strand, within EX28DRAFT_1916
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 1,924,556 + +0.2 1,924,664 + EX28DRAFT_1913 0.13 +0.3 1,924,665 - EX28DRAFT_1913 0.13 +0.0 1,924,703 - EX28DRAFT_1913 0.19 +0.8 1,924,931 - EX28DRAFT_1913 0.55 -0.1 1,925,023 + EX28DRAFT_1913 0.69 +0.1 1,925,023 + EX28DRAFT_1913 0.69 -0.1 1,925,024 - EX28DRAFT_1913 0.69 +0.5 1,925,045 - EX28DRAFT_1913 0.73 +1.0 1,925,047 + EX28DRAFT_1913 0.73 -0.0 1,925,189 + +0.3 1,925,384 - -0.2 1,925,446 + +0.1 1,925,446 + +0.7 1,925,447 - +0.2 1,925,478 + -0.8 1,925,479 - +1.3 1,925,531 + +0.8 1,925,532 - -0.2 1,925,554 + -0.4 1,925,714 + EX28DRAFT_1914 0.23 +0.2 1,925,715 - EX28DRAFT_1914 0.23 -0.2 1,925,837 + EX28DRAFT_1914 0.34 -0.2 1,925,904 - EX28DRAFT_1914 0.40 +0.4 1,925,904 - EX28DRAFT_1914 0.40 -1.2 1,926,176 + EX28DRAFT_1914 0.65 -0.0 1,926,176 + EX28DRAFT_1914 0.65 -0.5 1,926,239 + EX28DRAFT_1914 0.71 -0.4 1,926,386 + EX28DRAFT_1914 0.84 -2.3 1,927,090 + EX28DRAFT_1915 0.77 +0.0 1,927,341 + EX28DRAFT_1916 0.22 -0.0 1,927,341 + EX28DRAFT_1916 0.22 -1.9 1,927,342 - EX28DRAFT_1916 0.22 +0.2 1,927,342 - EX28DRAFT_1916 0.22 +0.0 1,927,389 - EX28DRAFT_1916 0.34 +0.5 1,927,512 + EX28DRAFT_1916 0.66 +0.7 1,927,533 + EX28DRAFT_1916 0.71 -0.6 1,927,533 + EX28DRAFT_1916 0.71 -0.2
Or see this region's nucleotide sequence