Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1660 and EX28DRAFT_1661 are separated by 36 nucleotides EX28DRAFT_1661 and EX28DRAFT_1662 are separated by 3 nucleotides EX28DRAFT_1662 and EX28DRAFT_1663 are separated by 4 nucleotides EX28DRAFT_1663 and EX28DRAFT_1664 are separated by 118 nucleotides EX28DRAFT_1664 and EX28DRAFT_1665 are separated by 28 nucleotides EX28DRAFT_1665 and EX28DRAFT_1666 are separated by 72 nucleotides
EX28DRAFT_1660: EX28DRAFT_1660 - putative efflux protein, MATE family, at 1,676,751 to 1,678,112
_1660
EX28DRAFT_1661: EX28DRAFT_1661 - hypothetical protein, at 1,678,149 to 1,678,262
_1661
EX28DRAFT_1662: EX28DRAFT_1662 - tRNA, at 1,678,266 to 1,678,342
_1662
EX28DRAFT_1663: EX28DRAFT_1663 - tRNA, at 1,678,347 to 1,678,423
_1663
EX28DRAFT_1664: EX28DRAFT_1664 - Uncharacterized protein conserved in bacteria, at 1,678,542 to 1,680,137
_1664
EX28DRAFT_1665: EX28DRAFT_1665 - Carbonic anhydrase, at 1,680,166 to 1,680,801
_1665
EX28DRAFT_1666: EX28DRAFT_1666 - hypothetical protein, at 1,680,874 to 1,681,656
_1666
Position (kb) 1678
1679
1680
1681 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1677.868 kb on + strand, within EX28DRAFT_1660 at 1677.881 kb on + strand, within EX28DRAFT_1660 at 1677.906 kb on - strand, within EX28DRAFT_1660 at 1677.949 kb on - strand, within EX28DRAFT_1660 at 1678.041 kb on - strand at 1678.482 kb on - strand at 1678.584 kb on + strand at 1678.585 kb on - strand at 1678.587 kb on - strand at 1678.671 kb on + strand at 1678.671 kb on + strand at 1678.672 kb on - strand at 1678.685 kb on + strand at 1678.746 kb on + strand, within EX28DRAFT_1664 at 1678.746 kb on + strand, within EX28DRAFT_1664 at 1678.883 kb on - strand, within EX28DRAFT_1664 at 1679.201 kb on + strand, within EX28DRAFT_1664 at 1679.202 kb on - strand, within EX28DRAFT_1664 at 1679.577 kb on + strand, within EX28DRAFT_1664 at 1679.578 kb on - strand, within EX28DRAFT_1664 at 1679.579 kb on + strand, within EX28DRAFT_1664 at 1679.686 kb on - strand, within EX28DRAFT_1664 at 1679.686 kb on - strand, within EX28DRAFT_1664 at 1679.804 kb on - strand, within EX28DRAFT_1664 at 1679.804 kb on - strand, within EX28DRAFT_1664 at 1679.952 kb on + strand, within EX28DRAFT_1664 at 1679.952 kb on + strand, within EX28DRAFT_1664 at 1679.953 kb on - strand, within EX28DRAFT_1664 at 1679.960 kb on - strand, within EX28DRAFT_1664 at 1680.168 kb on - strand at 1680.429 kb on - strand, within EX28DRAFT_1665 at 1680.817 kb on - strand at 1680.817 kb on - strand at 1680.862 kb on - strand at 1680.875 kb on + strand at 1680.876 kb on - strand at 1680.876 kb on - strand at 1680.876 kb on - strand at 1680.891 kb on + strand at 1680.891 kb on + strand at 1680.892 kb on - strand at 1680.892 kb on - strand at 1680.893 kb on + strand at 1680.894 kb on - strand at 1680.894 kb on - strand at 1680.894 kb on - strand at 1681.043 kb on + strand, within EX28DRAFT_1666 at 1681.053 kb on + strand, within EX28DRAFT_1666 at 1681.054 kb on - strand, within EX28DRAFT_1666 at 1681.109 kb on - strand, within EX28DRAFT_1666
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 1,677,868 + EX28DRAFT_1660 0.82 +0.2 1,677,881 + EX28DRAFT_1660 0.83 +0.5 1,677,906 - EX28DRAFT_1660 0.85 +0.4 1,677,949 - EX28DRAFT_1660 0.88 +0.2 1,678,041 - +0.7 1,678,482 - -1.1 1,678,584 + +1.1 1,678,585 - -1.5 1,678,587 - +0.1 1,678,671 + +0.8 1,678,671 + -1.3 1,678,672 - +0.6 1,678,685 + +0.2 1,678,746 + EX28DRAFT_1664 0.13 +0.3 1,678,746 + EX28DRAFT_1664 0.13 -1.7 1,678,883 - EX28DRAFT_1664 0.21 -0.9 1,679,201 + EX28DRAFT_1664 0.41 -0.9 1,679,202 - EX28DRAFT_1664 0.41 +0.5 1,679,577 + EX28DRAFT_1664 0.65 +0.5 1,679,578 - EX28DRAFT_1664 0.65 -0.2 1,679,579 + EX28DRAFT_1664 0.65 -0.2 1,679,686 - EX28DRAFT_1664 0.72 -1.0 1,679,686 - EX28DRAFT_1664 0.72 -0.1 1,679,804 - EX28DRAFT_1664 0.79 +0.9 1,679,804 - EX28DRAFT_1664 0.79 +0.7 1,679,952 + EX28DRAFT_1664 0.88 +0.2 1,679,952 + EX28DRAFT_1664 0.88 +0.7 1,679,953 - EX28DRAFT_1664 0.88 +1.2 1,679,960 - EX28DRAFT_1664 0.89 +1.3 1,680,168 - -0.4 1,680,429 - EX28DRAFT_1665 0.41 -0.6 1,680,817 - +0.3 1,680,817 - +1.8 1,680,862 - -0.2 1,680,875 + -1.4 1,680,876 - +0.0 1,680,876 - -1.3 1,680,876 - -0.4 1,680,891 + +0.2 1,680,891 + -0.4 1,680,892 - -1.2 1,680,892 - -2.2 1,680,893 + -0.9 1,680,894 - -0.2 1,680,894 - +0.0 1,680,894 - -0.4 1,681,043 + EX28DRAFT_1666 0.22 +0.2 1,681,053 + EX28DRAFT_1666 0.23 -1.4 1,681,054 - EX28DRAFT_1666 0.23 +0.5 1,681,109 - EX28DRAFT_1666 0.30 +0.0
Or see this region's nucleotide sequence