Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0545 and EX28DRAFT_0546 are separated by 210 nucleotides EX28DRAFT_0546 and EX28DRAFT_0547 are separated by 119 nucleotides EX28DRAFT_0547 and EX28DRAFT_0548 are separated by 92 nucleotides
EX28DRAFT_0545: EX28DRAFT_0545 - Exodeoxyribonuclease I subunit C (EC 3.1.11.1), at 539,030 to 540,454
_0545
EX28DRAFT_0546: EX28DRAFT_0546 - D-alanyl-D-alanine carboxypeptidase, at 540,665 to 541,831
_0546
EX28DRAFT_0547: EX28DRAFT_0547 - DNA gyrase inhibitor, at 541,951 to 542,424
_0547
EX28DRAFT_0548: EX28DRAFT_0548 - Predicted membrane protein, at 542,517 to 543,575
_0548
Position (kb) 540
541
542 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 539.731 kb on + strand, within EX28DRAFT_0545 at 540.462 kb on - strand at 540.489 kb on - strand at 540.489 kb on - strand at 540.493 kb on + strand at 540.597 kb on - strand at 540.597 kb on - strand at 540.660 kb on + strand at 540.660 kb on + strand at 540.660 kb on + strand at 540.661 kb on - strand at 540.661 kb on - strand at 540.841 kb on + strand, within EX28DRAFT_0546 at 540.875 kb on + strand, within EX28DRAFT_0546 at 540.876 kb on - strand, within EX28DRAFT_0546 at 541.259 kb on + strand, within EX28DRAFT_0546 at 541.259 kb on + strand, within EX28DRAFT_0546 at 541.264 kb on + strand, within EX28DRAFT_0546 at 541.264 kb on + strand, within EX28DRAFT_0546 at 541.264 kb on + strand, within EX28DRAFT_0546 at 541.265 kb on - strand, within EX28DRAFT_0546 at 541.265 kb on - strand, within EX28DRAFT_0546 at 541.265 kb on - strand, within EX28DRAFT_0546 at 541.265 kb on - strand, within EX28DRAFT_0546 at 541.268 kb on + strand, within EX28DRAFT_0546 at 541.268 kb on + strand, within EX28DRAFT_0546 at 541.268 kb on + strand, within EX28DRAFT_0546 at 541.269 kb on - strand, within EX28DRAFT_0546 at 541.271 kb on - strand, within EX28DRAFT_0546 at 541.333 kb on + strand, within EX28DRAFT_0546 at 541.369 kb on + strand, within EX28DRAFT_0546 at 541.369 kb on + strand, within EX28DRAFT_0546 at 541.370 kb on - strand, within EX28DRAFT_0546 at 541.514 kb on - strand, within EX28DRAFT_0546 at 541.558 kb on + strand, within EX28DRAFT_0546 at 541.724 kb on + strand at 541.725 kb on - strand at 541.725 kb on - strand at 541.830 kb on - strand at 541.873 kb on + strand at 541.873 kb on + strand at 541.873 kb on + strand at 541.874 kb on - strand at 541.958 kb on - strand at 541.983 kb on + strand at 541.984 kb on - strand at 541.984 kb on - strand at 542.105 kb on - strand, within EX28DRAFT_0547 at 542.105 kb on - strand, within EX28DRAFT_0547 at 542.293 kb on - strand, within EX28DRAFT_0547 at 542.293 kb on - strand, within EX28DRAFT_0547 at 542.313 kb on + strand, within EX28DRAFT_0547 at 542.313 kb on + strand, within EX28DRAFT_0547 at 542.313 kb on + strand, within EX28DRAFT_0547 at 542.314 kb on - strand, within EX28DRAFT_0547 at 542.318 kb on - strand, within EX28DRAFT_0547 at 542.395 kb on + strand at 542.395 kb on + strand at 542.395 kb on + strand at 542.395 kb on + strand at 542.396 kb on - strand at 542.396 kb on - strand at 542.412 kb on + strand at 542.413 kb on - strand at 542.501 kb on + strand at 542.537 kb on - strand at 542.559 kb on + strand at 542.560 kb on - strand at 542.620 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 539,731 + EX28DRAFT_0545 0.49 -1.5 540,462 - -1.2 540,489 - +0.2 540,489 - +0.5 540,493 + -0.1 540,597 - -1.9 540,597 - +0.3 540,660 + +0.0 540,660 + -0.3 540,660 + +0.2 540,661 - -0.1 540,661 - -0.6 540,841 + EX28DRAFT_0546 0.15 -0.2 540,875 + EX28DRAFT_0546 0.18 -0.6 540,876 - EX28DRAFT_0546 0.18 +0.1 541,259 + EX28DRAFT_0546 0.51 -0.1 541,259 + EX28DRAFT_0546 0.51 +0.1 541,264 + EX28DRAFT_0546 0.51 -0.2 541,264 + EX28DRAFT_0546 0.51 -0.1 541,264 + EX28DRAFT_0546 0.51 -1.0 541,265 - EX28DRAFT_0546 0.51 -0.7 541,265 - EX28DRAFT_0546 0.51 +0.1 541,265 - EX28DRAFT_0546 0.51 +0.5 541,265 - EX28DRAFT_0546 0.51 -0.7 541,268 + EX28DRAFT_0546 0.52 -0.1 541,268 + EX28DRAFT_0546 0.52 -0.1 541,268 + EX28DRAFT_0546 0.52 -0.3 541,269 - EX28DRAFT_0546 0.52 +0.2 541,271 - EX28DRAFT_0546 0.52 -0.5 541,333 + EX28DRAFT_0546 0.57 +0.5 541,369 + EX28DRAFT_0546 0.60 -0.2 541,369 + EX28DRAFT_0546 0.60 -0.8 541,370 - EX28DRAFT_0546 0.60 +0.1 541,514 - EX28DRAFT_0546 0.73 -0.0 541,558 + EX28DRAFT_0546 0.77 -0.0 541,724 + +0.5 541,725 - +0.1 541,725 - +0.2 541,830 - +0.9 541,873 + +0.1 541,873 + -0.3 541,873 + +0.1 541,874 - -0.5 541,958 - +0.3 541,983 + -0.6 541,984 - +0.3 541,984 - -1.9 542,105 - EX28DRAFT_0547 0.32 -0.1 542,105 - EX28DRAFT_0547 0.32 +0.3 542,293 - EX28DRAFT_0547 0.72 -0.8 542,293 - EX28DRAFT_0547 0.72 +0.0 542,313 + EX28DRAFT_0547 0.76 +0.2 542,313 + EX28DRAFT_0547 0.76 -0.8 542,313 + EX28DRAFT_0547 0.76 +0.7 542,314 - EX28DRAFT_0547 0.77 +0.9 542,318 - EX28DRAFT_0547 0.77 +0.1 542,395 + -0.1 542,395 + +1.1 542,395 + +0.4 542,395 + -0.6 542,396 - +0.4 542,396 - -3.8 542,412 + -0.3 542,413 - +0.8 542,501 + +0.2 542,537 - -0.1 542,559 + +0.1 542,560 - -0.9 542,620 + +0.9
Or see this region's nucleotide sequence