Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0423 and EX28DRAFT_0424 are separated by 84 nucleotides EX28DRAFT_0424 and EX28DRAFT_0425 are separated by 157 nucleotides EX28DRAFT_0425 and EX28DRAFT_0426 are separated by 167 nucleotides
EX28DRAFT_0423: EX28DRAFT_0423 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 403,089 to 403,853
_0423
EX28DRAFT_0424: EX28DRAFT_0424 - Uncharacterized membrane-associated protein, at 403,938 to 404,510
_0424
EX28DRAFT_0425: EX28DRAFT_0425 - Protein of unknown function (DUF1282), at 404,668 to 405,255
_0425
EX28DRAFT_0426: EX28DRAFT_0426 - D-alanyl-D-alanine carboxypeptidase, at 405,423 to 406,346
_0426
Position (kb) 404
405
406 Strain fitness (log2 ratio) -1
0
1
2 at 403.799 kb on + strand at 403.852 kb on - strand at 403.911 kb on - strand at 404.207 kb on + strand, within EX28DRAFT_0424 at 404.207 kb on + strand, within EX28DRAFT_0424 at 404.208 kb on - strand, within EX28DRAFT_0424 at 404.208 kb on - strand, within EX28DRAFT_0424 at 404.208 kb on - strand, within EX28DRAFT_0424 at 404.234 kb on - strand, within EX28DRAFT_0424 at 404.332 kb on + strand, within EX28DRAFT_0424 at 404.492 kb on + strand at 404.493 kb on - strand at 404.511 kb on + strand at 404.511 kb on + strand at 404.511 kb on + strand at 404.523 kb on - strand at 404.523 kb on - strand at 404.546 kb on - strand at 404.551 kb on + strand at 404.551 kb on + strand at 404.551 kb on + strand at 404.551 kb on + strand at 404.552 kb on - strand at 404.552 kb on - strand at 404.553 kb on + strand at 404.553 kb on + strand at 404.553 kb on + strand at 404.554 kb on - strand at 404.554 kb on - strand at 404.625 kb on + strand at 404.625 kb on + strand at 404.627 kb on + strand at 404.627 kb on + strand at 404.627 kb on + strand at 404.628 kb on - strand at 404.628 kb on - strand at 404.628 kb on - strand at 404.631 kb on + strand at 404.632 kb on - strand at 404.632 kb on - strand at 404.792 kb on + strand, within EX28DRAFT_0425 at 404.792 kb on + strand, within EX28DRAFT_0425 at 405.023 kb on + strand, within EX28DRAFT_0425 at 405.078 kb on + strand, within EX28DRAFT_0425 at 405.079 kb on - strand, within EX28DRAFT_0425 at 405.079 kb on - strand, within EX28DRAFT_0425 at 405.091 kb on + strand, within EX28DRAFT_0425 at 405.092 kb on - strand, within EX28DRAFT_0425 at 405.092 kb on - strand, within EX28DRAFT_0425 at 405.109 kb on + strand, within EX28DRAFT_0425 at 405.142 kb on - strand, within EX28DRAFT_0425 at 405.276 kb on - strand at 405.383 kb on + strand at 405.403 kb on + strand at 405.404 kb on - strand at 405.450 kb on - strand at 405.578 kb on + strand, within EX28DRAFT_0426 at 405.588 kb on + strand, within EX28DRAFT_0426 at 405.589 kb on - strand, within EX28DRAFT_0426 at 405.637 kb on + strand, within EX28DRAFT_0426 at 405.730 kb on - strand, within EX28DRAFT_0426 at 405.906 kb on + strand, within EX28DRAFT_0426 at 405.907 kb on - strand, within EX28DRAFT_0426 at 406.052 kb on + strand, within EX28DRAFT_0426 at 406.053 kb on - strand, within EX28DRAFT_0426 at 406.053 kb on - strand, within EX28DRAFT_0426 at 406.053 kb on - strand, within EX28DRAFT_0426 at 406.053 kb on - strand, within EX28DRAFT_0426 at 406.053 kb on - strand, within EX28DRAFT_0426 at 406.053 kb on - strand, within EX28DRAFT_0426 at 406.074 kb on - strand, within EX28DRAFT_0426 at 406.084 kb on + strand, within EX28DRAFT_0426 at 406.086 kb on + strand, within EX28DRAFT_0426 at 406.176 kb on - strand, within EX28DRAFT_0426 at 406.215 kb on + strand, within EX28DRAFT_0426 at 406.215 kb on + strand, within EX28DRAFT_0426 at 406.215 kb on + strand, within EX28DRAFT_0426 at 406.215 kb on + strand, within EX28DRAFT_0426 at 406.215 kb on + strand, within EX28DRAFT_0426 at 406.216 kb on - strand, within EX28DRAFT_0426 at 406.247 kb on + strand, within EX28DRAFT_0426 at 406.248 kb on - strand, within EX28DRAFT_0426
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 403,799 + +2.4 403,852 - -0.1 403,911 - -0.2 404,207 + EX28DRAFT_0424 0.47 +0.1 404,207 + EX28DRAFT_0424 0.47 -0.3 404,208 - EX28DRAFT_0424 0.47 -0.3 404,208 - EX28DRAFT_0424 0.47 +0.0 404,208 - EX28DRAFT_0424 0.47 -0.7 404,234 - EX28DRAFT_0424 0.52 +0.4 404,332 + EX28DRAFT_0424 0.69 -0.5 404,492 + -0.1 404,493 - -0.3 404,511 + -0.4 404,511 + +0.1 404,511 + +0.7 404,523 - +0.4 404,523 - +0.3 404,546 - -0.3 404,551 + +1.2 404,551 + -0.0 404,551 + +0.2 404,551 + +0.7 404,552 - +0.1 404,552 - -0.5 404,553 + -0.1 404,553 + +0.0 404,553 + -0.6 404,554 - -0.2 404,554 - -0.5 404,625 + -0.2 404,625 + -0.2 404,627 + +0.3 404,627 + +0.8 404,627 + -0.4 404,628 - -0.0 404,628 - +0.5 404,628 - -0.2 404,631 + +0.1 404,632 - +0.6 404,632 - -0.0 404,792 + EX28DRAFT_0425 0.21 -0.2 404,792 + EX28DRAFT_0425 0.21 +1.2 405,023 + EX28DRAFT_0425 0.60 +0.1 405,078 + EX28DRAFT_0425 0.70 -0.0 405,079 - EX28DRAFT_0425 0.70 +0.3 405,079 - EX28DRAFT_0425 0.70 +0.6 405,091 + EX28DRAFT_0425 0.72 +1.0 405,092 - EX28DRAFT_0425 0.72 +0.7 405,092 - EX28DRAFT_0425 0.72 +0.1 405,109 + EX28DRAFT_0425 0.75 -0.8 405,142 - EX28DRAFT_0425 0.81 +0.2 405,276 - -0.5 405,383 + -0.3 405,403 + -1.5 405,404 - -0.1 405,450 - +0.9 405,578 + EX28DRAFT_0426 0.17 -1.4 405,588 + EX28DRAFT_0426 0.18 +0.4 405,589 - EX28DRAFT_0426 0.18 +0.1 405,637 + EX28DRAFT_0426 0.23 +0.2 405,730 - EX28DRAFT_0426 0.33 -0.8 405,906 + EX28DRAFT_0426 0.52 +0.2 405,907 - EX28DRAFT_0426 0.52 +0.0 406,052 + EX28DRAFT_0426 0.68 -0.4 406,053 - EX28DRAFT_0426 0.68 +0.1 406,053 - EX28DRAFT_0426 0.68 +0.2 406,053 - EX28DRAFT_0426 0.68 +0.3 406,053 - EX28DRAFT_0426 0.68 +0.3 406,053 - EX28DRAFT_0426 0.68 -0.1 406,053 - EX28DRAFT_0426 0.68 +0.6 406,074 - EX28DRAFT_0426 0.70 +0.0 406,084 + EX28DRAFT_0426 0.72 +0.1 406,086 + EX28DRAFT_0426 0.72 +0.4 406,176 - EX28DRAFT_0426 0.81 -0.6 406,215 + EX28DRAFT_0426 0.86 +0.1 406,215 + EX28DRAFT_0426 0.86 +0.4 406,215 + EX28DRAFT_0426 0.86 -0.2 406,215 + EX28DRAFT_0426 0.86 -0.6 406,215 + EX28DRAFT_0426 0.86 +0.4 406,216 - EX28DRAFT_0426 0.86 +0.3 406,247 + EX28DRAFT_0426 0.89 +1.1 406,248 - EX28DRAFT_0426 0.89 -0.3
Or see this region's nucleotide sequence