Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0004 and EX28DRAFT_0005 are separated by 341 nucleotides EX28DRAFT_0005 and EX28DRAFT_0006 are separated by 32 nucleotides EX28DRAFT_0006 and EX28DRAFT_0007 are separated by 10 nucleotides EX28DRAFT_0007 and EX28DRAFT_0008 overlap by 4 nucleotides EX28DRAFT_0008 and EX28DRAFT_0009 are separated by 17 nucleotides
EX28DRAFT_0004: EX28DRAFT_0004 - Type IV leader peptidase family, at 1,411 to 1,923
_0004
EX28DRAFT_0005: EX28DRAFT_0005 - SSU ribosomal protein S10P, at 2,265 to 2,576
_0005
EX28DRAFT_0006: EX28DRAFT_0006 - 50S ribosomal protein L3, bacterial, at 2,609 to 3,238
_0006
EX28DRAFT_0007: EX28DRAFT_0007 - LSU ribosomal protein L4P, at 3,249 to 3,854
_0007
EX28DRAFT_0008: EX28DRAFT_0008 - LSU ribosomal protein L23P, at 3,851 to 4,153
_0008
EX28DRAFT_0009: EX28DRAFT_0009 - ribosomal protein L2, bacterial/organellar, at 4,171 to 4,992
_0009
Position (kb) 2
3
4 Strain fitness (log2 ratio) -1
0
1 at 1.625 kb on - strand, within EX28DRAFT_0004 at 1.677 kb on - strand, within EX28DRAFT_0004 at 1.677 kb on - strand, within EX28DRAFT_0004 at 1.698 kb on - strand, within EX28DRAFT_0004 at 1.818 kb on + strand, within EX28DRAFT_0004 at 1.994 kb on + strand at 1.995 kb on - strand at 1.995 kb on - strand at 2.027 kb on + strand at 2.028 kb on - strand at 2.028 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 1,625 - EX28DRAFT_0004 0.42 -0.7 1,677 - EX28DRAFT_0004 0.52 -0.0 1,677 - EX28DRAFT_0004 0.52 +0.8 1,698 - EX28DRAFT_0004 0.56 -0.3 1,818 + EX28DRAFT_0004 0.79 -0.2 1,994 + +1.1 1,995 - +1.2 1,995 - -0.6 2,027 + +0.9 2,028 - -0.6 2,028 - -0.7
Or see this region's nucleotide sequence