Experiment: L-Asparagine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS01245 and ABID97_RS01250 overlap by 4 nucleotides ABID97_RS01250 and ABID97_RS01255 overlap by 14 nucleotides ABID97_RS01255 and ABID97_RS01260 overlap by 4 nucleotides ABID97_RS01260 and ABID97_RS01265 are separated by 146 nucleotides
ABID97_RS01245: ABID97_RS01245 - 5-oxoprolinase subunit PxpB, at 255,609 to 256,355
_RS01245
ABID97_RS01250: ABID97_RS01250 - biotin-dependent carboxyltransferase family protein, at 256,352 to 257,302
_RS01250
ABID97_RS01255: ABID97_RS01255 - 5-oxoprolinase subunit PxpA, at 257,289 to 258,101
_RS01255
ABID97_RS01260: ABID97_RS01260 - acetyl-CoA carboxylase biotin carboxyl carrier protein subunit, at 258,098 to 258,325
_RS01260
ABID97_RS01265: ABID97_RS01265 - MFS transporter, at 258,472 to 259,824
_RS01265
Position (kb) 257
258
259 Strain fitness (log2 ratio) -1
0
1 at 256.685 kb on + strand, within ABID97_RS01250 at 256.686 kb on - strand, within ABID97_RS01250 at 256.686 kb on - strand, within ABID97_RS01250 at 256.742 kb on + strand, within ABID97_RS01250 at 256.743 kb on - strand, within ABID97_RS01250 at 257.328 kb on + strand at 257.338 kb on - strand at 257.634 kb on + strand, within ABID97_RS01255 at 257.715 kb on + strand, within ABID97_RS01255 at 257.716 kb on - strand, within ABID97_RS01255 at 257.799 kb on + strand, within ABID97_RS01255 at 257.799 kb on + strand, within ABID97_RS01255 at 257.800 kb on - strand, within ABID97_RS01255 at 258.619 kb on + strand, within ABID97_RS01265 at 258.619 kb on + strand, within ABID97_RS01265 at 258.640 kb on + strand, within ABID97_RS01265 at 258.994 kb on + strand, within ABID97_RS01265 at 258.994 kb on + strand, within ABID97_RS01265
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (C) remove 256,685 + ABID97_RS01250 0.35 -0.2 256,686 - ABID97_RS01250 0.35 +0.4 256,686 - ABID97_RS01250 0.35 -0.4 256,742 + ABID97_RS01250 0.41 +0.7 256,743 - ABID97_RS01250 0.41 +0.2 257,328 + +0.7 257,338 - +0.1 257,634 + ABID97_RS01255 0.42 +0.1 257,715 + ABID97_RS01255 0.52 +0.2 257,716 - ABID97_RS01255 0.53 +0.3 257,799 + ABID97_RS01255 0.63 -0.1 257,799 + ABID97_RS01255 0.63 +1.6 257,800 - ABID97_RS01255 0.63 +0.2 258,619 + ABID97_RS01265 0.11 -0.7 258,619 + ABID97_RS01265 0.11 +0.8 258,640 + ABID97_RS01265 0.12 +0.9 258,994 + ABID97_RS01265 0.39 -0.3 258,994 + ABID97_RS01265 0.39 -0.2
Or see this region's nucleotide sequence