Strain Fitness in Pseudomonas fluorescens SBW25 around PFLU_RS01630

Experiment: succinate (C); with MOPS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS01620 and PFLU_RS01625 are separated by 9 nucleotidesPFLU_RS01625 and PFLU_RS01630 are separated by 66 nucleotidesPFLU_RS01630 and PFLU_RS01635 are separated by 3 nucleotides PFLU_RS01620: PFLU_RS01620 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 362,169 to 362,906 _RS01620 PFLU_RS01625: PFLU_RS01625 - imidazole glycerol phosphate synthase subunit HisF, at 362,916 to 363,686 _RS01625 PFLU_RS01630: PFLU_RS01630 - ABC transporter substrate-binding protein, at 363,753 to 364,508 _RS01630 PFLU_RS01635: PFLU_RS01635 - serine protease, at 364,512 to 366,077 _RS01635 Position (kb) 363 364 365Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 362.847 kb on + strandat 362.847 kb on + strandat 362.847 kb on + strandat 362.847 kb on + strandat 362.848 kb on - strandat 362.855 kb on + strandat 362.856 kb on - strandat 362.883 kb on + strandat 362.883 kb on + strandat 362.884 kb on - strandat 362.895 kb on + strandat 362.895 kb on + strandat 363.060 kb on - strand, within PFLU_RS01625at 363.060 kb on - strand, within PFLU_RS01625at 363.110 kb on + strand, within PFLU_RS01625at 363.612 kb on - strandat 363.631 kb on - strandat 363.631 kb on - strandat 363.631 kb on - strandat 363.654 kb on + strandat 363.654 kb on + strandat 363.655 kb on - strandat 363.655 kb on - strandat 363.655 kb on - strandat 363.655 kb on - strandat 363.655 kb on - strandat 363.655 kb on - strandat 363.785 kb on + strandat 363.786 kb on - strandat 363.786 kb on - strandat 363.786 kb on - strandat 363.870 kb on + strand, within PFLU_RS01630at 363.870 kb on + strand, within PFLU_RS01630at 363.972 kb on + strand, within PFLU_RS01630at 363.972 kb on + strand, within PFLU_RS01630at 364.008 kb on + strand, within PFLU_RS01630at 364.009 kb on - strand, within PFLU_RS01630at 364.009 kb on - strand, within PFLU_RS01630at 364.036 kb on - strand, within PFLU_RS01630at 364.137 kb on - strand, within PFLU_RS01630at 364.137 kb on - strand, within PFLU_RS01630at 364.137 kb on - strand, within PFLU_RS01630at 364.137 kb on - strand, within PFLU_RS01630at 364.176 kb on + strand, within PFLU_RS01630at 364.191 kb on + strand, within PFLU_RS01630at 364.193 kb on + strand, within PFLU_RS01630at 364.194 kb on - strand, within PFLU_RS01630at 364.235 kb on + strand, within PFLU_RS01630at 364.236 kb on - strand, within PFLU_RS01630at 364.380 kb on - strand, within PFLU_RS01630at 364.380 kb on - strand, within PFLU_RS01630at 364.396 kb on - strand, within PFLU_RS01630at 364.500 kb on + strandat 364.500 kb on + strandat 364.501 kb on - strandat 364.501 kb on - strandat 364.501 kb on - strandat 364.506 kb on + strandat 364.507 kb on - strandat 364.573 kb on + strandat 364.573 kb on + strandat 364.573 kb on + strandat 364.573 kb on + strandat 364.574 kb on - strandat 364.574 kb on - strandat 364.574 kb on - strandat 364.610 kb on + strandat 364.611 kb on - strandat 364.777 kb on + strand, within PFLU_RS01635at 364.777 kb on + strand, within PFLU_RS01635at 364.777 kb on + strand, within PFLU_RS01635at 364.778 kb on - strand, within PFLU_RS01635at 364.778 kb on - strand, within PFLU_RS01635at 364.778 kb on - strand, within PFLU_RS01635at 364.783 kb on + strand, within PFLU_RS01635at 364.784 kb on - strand, within PFLU_RS01635at 364.793 kb on + strand, within PFLU_RS01635at 364.793 kb on + strand, within PFLU_RS01635at 364.793 kb on + strand, within PFLU_RS01635at 364.794 kb on - strand, within PFLU_RS01635at 364.794 kb on - strand, within PFLU_RS01635at 364.825 kb on + strand, within PFLU_RS01635at 364.825 kb on + strand, within PFLU_RS01635at 364.825 kb on + strand, within PFLU_RS01635at 364.826 kb on - strand, within PFLU_RS01635at 364.826 kb on - strand, within PFLU_RS01635at 365.044 kb on + strand, within PFLU_RS01635at 365.044 kb on + strand, within PFLU_RS01635at 365.045 kb on - strand, within PFLU_RS01635at 365.079 kb on + strand, within PFLU_RS01635at 365.089 kb on + strand, within PFLU_RS01635at 365.089 kb on + strand, within PFLU_RS01635at 365.090 kb on - strand, within PFLU_RS01635at 365.090 kb on - strand, within PFLU_RS01635at 365.090 kb on - strand, within PFLU_RS01635at 365.111 kb on + strand, within PFLU_RS01635at 365.142 kb on - strand, within PFLU_RS01635at 365.176 kb on + strand, within PFLU_RS01635at 365.176 kb on + strand, within PFLU_RS01635at 365.176 kb on + strand, within PFLU_RS01635at 365.177 kb on - strand, within PFLU_RS01635at 365.177 kb on - strand, within PFLU_RS01635at 365.177 kb on - strand, within PFLU_RS01635at 365.177 kb on - strand, within PFLU_RS01635at 365.224 kb on + strand, within PFLU_RS01635at 365.302 kb on + strand, within PFLU_RS01635at 365.302 kb on + strand, within PFLU_RS01635at 365.303 kb on - strand, within PFLU_RS01635at 365.303 kb on - strand, within PFLU_RS01635at 365.303 kb on - strand, within PFLU_RS01635at 365.303 kb on - strand, within PFLU_RS01635at 365.303 kb on - strand, within PFLU_RS01635at 365.303 kb on - strand, within PFLU_RS01635at 365.303 kb on - strand, within PFLU_RS01635at 365.306 kb on + strand, within PFLU_RS01635at 365.306 kb on + strand, within PFLU_RS01635at 365.306 kb on + strand, within PFLU_RS01635at 365.306 kb on + strand, within PFLU_RS01635at 365.306 kb on + strand, within PFLU_RS01635at 365.306 kb on + strand, within PFLU_RS01635at 365.307 kb on - strand, within PFLU_RS01635at 365.396 kb on + strand, within PFLU_RS01635at 365.396 kb on + strand, within PFLU_RS01635at 365.396 kb on + strand, within PFLU_RS01635at 365.396 kb on + strand, within PFLU_RS01635at 365.397 kb on - strand, within PFLU_RS01635at 365.397 kb on - strand, within PFLU_RS01635at 365.421 kb on - strand, within PFLU_RS01635at 365.421 kb on - strand, within PFLU_RS01635at 365.421 kb on - strand, within PFLU_RS01635at 365.421 kb on - strand, within PFLU_RS01635at 365.494 kb on - strand, within PFLU_RS01635

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Per-strain Table

Position Strand Gene LocusTag Fraction succinate (C); with MOPS
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362,847 + -3.1
362,847 + -2.6
362,847 + -3.5
362,847 + -0.6
362,848 - -2.9
362,855 + -2.1
362,856 - -3.2
362,883 + -1.3
362,883 + -1.5
362,884 - -1.7
362,895 + -2.0
362,895 + +0.4
363,060 - PFLU_RS01625 0.19 -0.6
363,060 - PFLU_RS01625 0.19 -1.9
363,110 + PFLU_RS01625 0.25 -1.8
363,612 - -1.7
363,631 - -0.8
363,631 - -1.2
363,631 - -1.8
363,654 + -2.7
363,654 + -2.9
363,655 - -2.3
363,655 - -0.4
363,655 - -2.7
363,655 - -0.9
363,655 - +1.6
363,655 - +1.4
363,785 + +0.5
363,786 - +1.2
363,786 - -0.4
363,786 - -0.3
363,870 + PFLU_RS01630 0.15 +0.4
363,870 + PFLU_RS01630 0.15 +0.0
363,972 + PFLU_RS01630 0.29 -1.2
363,972 + PFLU_RS01630 0.29 +2.2
364,008 + PFLU_RS01630 0.34 +0.6
364,009 - PFLU_RS01630 0.34 +0.9
364,009 - PFLU_RS01630 0.34 +0.6
364,036 - PFLU_RS01630 0.37 +0.4
364,137 - PFLU_RS01630 0.51 +1.3
364,137 - PFLU_RS01630 0.51 +0.2
364,137 - PFLU_RS01630 0.51 +0.1
364,137 - PFLU_RS01630 0.51 -0.1
364,176 + PFLU_RS01630 0.56 -0.7
364,191 + PFLU_RS01630 0.58 +0.6
364,193 + PFLU_RS01630 0.58 -0.2
364,194 - PFLU_RS01630 0.58 -0.1
364,235 + PFLU_RS01630 0.64 -0.3
364,236 - PFLU_RS01630 0.64 +0.1
364,380 - PFLU_RS01630 0.83 +0.5
364,380 - PFLU_RS01630 0.83 +0.7
364,396 - PFLU_RS01630 0.85 +1.5
364,500 + -0.3
364,500 + -0.2
364,501 - -0.9
364,501 - +1.2
364,501 - +1.3
364,506 + -0.2
364,507 - +2.4
364,573 + -0.8
364,573 + -1.4
364,573 + +0.6
364,573 + -0.7
364,574 - -1.1
364,574 - -0.6
364,574 - +0.4
364,610 + -0.1
364,611 - -0.2
364,777 + PFLU_RS01635 0.17 -0.2
364,777 + PFLU_RS01635 0.17 +0.1
364,777 + PFLU_RS01635 0.17 +1.1
364,778 - PFLU_RS01635 0.17 -0.2
364,778 - PFLU_RS01635 0.17 +0.6
364,778 - PFLU_RS01635 0.17 +0.5
364,783 + PFLU_RS01635 0.17 -0.9
364,784 - PFLU_RS01635 0.17 +0.2
364,793 + PFLU_RS01635 0.18 -0.0
364,793 + PFLU_RS01635 0.18 +0.4
364,793 + PFLU_RS01635 0.18 +0.1
364,794 - PFLU_RS01635 0.18 -0.0
364,794 - PFLU_RS01635 0.18 +0.2
364,825 + PFLU_RS01635 0.20 -0.5
364,825 + PFLU_RS01635 0.20 +0.7
364,825 + PFLU_RS01635 0.20 +0.3
364,826 - PFLU_RS01635 0.20 -0.1
364,826 - PFLU_RS01635 0.20 -0.2
365,044 + PFLU_RS01635 0.34 +1.0
365,044 + PFLU_RS01635 0.34 -0.1
365,045 - PFLU_RS01635 0.34 +0.1
365,079 + PFLU_RS01635 0.36 -0.6
365,089 + PFLU_RS01635 0.37 -1.0
365,089 + PFLU_RS01635 0.37 -0.1
365,090 - PFLU_RS01635 0.37 +0.4
365,090 - PFLU_RS01635 0.37 -0.6
365,090 - PFLU_RS01635 0.37 +0.3
365,111 + PFLU_RS01635 0.38 +0.2
365,142 - PFLU_RS01635 0.40 +0.5
365,176 + PFLU_RS01635 0.42 +0.4
365,176 + PFLU_RS01635 0.42 -0.6
365,176 + PFLU_RS01635 0.42 -0.9
365,177 - PFLU_RS01635 0.42 -0.6
365,177 - PFLU_RS01635 0.42 -0.6
365,177 - PFLU_RS01635 0.42 -0.3
365,177 - PFLU_RS01635 0.42 +0.2
365,224 + PFLU_RS01635 0.45 +0.4
365,302 + PFLU_RS01635 0.50 -0.2
365,302 + PFLU_RS01635 0.50 +0.3
365,303 - PFLU_RS01635 0.51 -1.3
365,303 - PFLU_RS01635 0.51 +0.9
365,303 - PFLU_RS01635 0.51 +0.5
365,303 - PFLU_RS01635 0.51 -0.4
365,303 - PFLU_RS01635 0.51 +0.2
365,303 - PFLU_RS01635 0.51 +0.3
365,303 - PFLU_RS01635 0.51 +0.9
365,306 + PFLU_RS01635 0.51 -0.1
365,306 + PFLU_RS01635 0.51 +0.1
365,306 + PFLU_RS01635 0.51 +1.1
365,306 + PFLU_RS01635 0.51 -1.0
365,306 + PFLU_RS01635 0.51 -0.2
365,306 + PFLU_RS01635 0.51 +1.7
365,307 - PFLU_RS01635 0.51 -1.3
365,396 + PFLU_RS01635 0.56 -0.4
365,396 + PFLU_RS01635 0.56 -1.4
365,396 + PFLU_RS01635 0.56 -1.2
365,396 + PFLU_RS01635 0.56 +0.5
365,397 - PFLU_RS01635 0.57 +0.7
365,397 - PFLU_RS01635 0.57 -1.3
365,421 - PFLU_RS01635 0.58 +1.6
365,421 - PFLU_RS01635 0.58 +0.6
365,421 - PFLU_RS01635 0.58 +1.2
365,421 - PFLU_RS01635 0.58 -1.1
365,494 - PFLU_RS01635 0.63 +0.2

Or see this region's nucleotide sequence