Experiment: PYE with Dimethyl Sulfoxide 0.4 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03028 and CCNA_03029 overlap by 4 nucleotides CCNA_03029 and CCNA_03030 are separated by 156 nucleotides CCNA_03030 and CCNA_03031 are separated by 213 nucleotides
CCNA_03028: CCNA_03028 - prephenate dehydratase, at 3,183,110 to 3,183,961
_03028
CCNA_03029: CCNA_03029 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 3,183,958 to 3,184,695
_03029
CCNA_03030: CCNA_03030 - cytochrome c2, at 3,184,852 to 3,185,538
_03030
CCNA_03031: CCNA_03031 - NlpC/P60 family protein, at 3,185,752 to 3,186,591
_03031
Position (kb)
3184
3185
3186 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3183.858 kb on + strand, within CCNA_03028 at 3183.858 kb on - strand, within CCNA_03028 at 3183.858 kb on - strand, within CCNA_03028 at 3183.858 kb on - strand, within CCNA_03028 at 3183.858 kb on - strand, within CCNA_03028 at 3183.866 kb on + strand, within CCNA_03028 at 3183.874 kb on - strand, within CCNA_03028 at 3183.882 kb on - strand at 3183.912 kb on - strand at 3183.912 kb on - strand at 3183.945 kb on + strand at 3183.953 kb on - strand at 3183.955 kb on + strand at 3183.968 kb on + strand at 3184.059 kb on - strand, within CCNA_03029 at 3184.698 kb on + strand at 3184.706 kb on - strand at 3184.706 kb on - strand at 3184.706 kb on - strand at 3184.716 kb on - strand at 3184.721 kb on - strand at 3184.728 kb on + strand at 3184.802 kb on + strand at 3184.810 kb on - strand at 3184.815 kb on + strand at 3184.950 kb on + strand, within CCNA_03030 at 3185.042 kb on - strand, within CCNA_03030 at 3185.185 kb on + strand, within CCNA_03030 at 3185.373 kb on - strand, within CCNA_03030 at 3185.499 kb on - strand at 3185.532 kb on + strand at 3185.533 kb on + strand at 3185.662 kb on - strand at 3185.667 kb on - strand at 3185.669 kb on - strand at 3185.677 kb on - strand at 3185.690 kb on - strand at 3185.775 kb on - strand at 3185.818 kb on - strand at 3185.998 kb on + strand, within CCNA_03031 at 3186.006 kb on - strand, within CCNA_03031 at 3186.006 kb on - strand, within CCNA_03031 at 3186.021 kb on + strand, within CCNA_03031 at 3186.031 kb on + strand, within CCNA_03031 at 3186.039 kb on - strand, within CCNA_03031 at 3186.039 kb on - strand, within CCNA_03031 at 3186.039 kb on - strand, within CCNA_03031 at 3186.063 kb on + strand, within CCNA_03031 at 3186.071 kb on - strand, within CCNA_03031 at 3186.192 kb on + strand, within CCNA_03031 at 3186.277 kb on - strand, within CCNA_03031 at 3186.338 kb on - strand, within CCNA_03031 at 3186.383 kb on + strand, within CCNA_03031 at 3186.406 kb on - strand, within CCNA_03031
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Dimethyl Sulfoxide 0.4 % remove 3,183,858 + CCNA_03028 0.88 -1.2 3,183,858 - CCNA_03028 0.88 +0.5 3,183,858 - CCNA_03028 0.88 +0.4 3,183,858 - CCNA_03028 0.88 +0.8 3,183,858 - CCNA_03028 0.88 +0.3 3,183,866 + CCNA_03028 0.89 +0.2 3,183,874 - CCNA_03028 0.90 +0.4 3,183,882 - +1.7 3,183,912 - +1.0 3,183,912 - +0.3 3,183,945 + +1.0 3,183,953 - +0.5 3,183,955 + -0.6 3,183,968 + -0.7 3,184,059 - CCNA_03029 0.14 +0.7 3,184,698 + -0.5 3,184,706 - +0.4 3,184,706 - -0.6 3,184,706 - +0.1 3,184,716 - +0.2 3,184,721 - +1.6 3,184,728 + +0.9 3,184,802 + +1.4 3,184,810 - -0.3 3,184,815 + -0.2 3,184,950 + CCNA_03030 0.14 +0.0 3,185,042 - CCNA_03030 0.28 -2.4 3,185,185 + CCNA_03030 0.48 -2.9 3,185,373 - CCNA_03030 0.76 +0.2 3,185,499 - -0.6 3,185,532 + +0.7 3,185,533 + +0.2 3,185,662 - +1.1 3,185,667 - +0.5 3,185,669 - -0.5 3,185,677 - +0.5 3,185,690 - +0.5 3,185,775 - +0.9 3,185,818 - +0.2 3,185,998 + CCNA_03031 0.29 +0.6 3,186,006 - CCNA_03031 0.30 +1.2 3,186,006 - CCNA_03031 0.30 +0.0 3,186,021 + CCNA_03031 0.32 -0.2 3,186,031 + CCNA_03031 0.33 +0.7 3,186,039 - CCNA_03031 0.34 +0.9 3,186,039 - CCNA_03031 0.34 -0.7 3,186,039 - CCNA_03031 0.34 +0.5 3,186,063 + CCNA_03031 0.37 +0.5 3,186,071 - CCNA_03031 0.38 -0.1 3,186,192 + CCNA_03031 0.52 +1.7 3,186,277 - CCNA_03031 0.62 -0.4 3,186,338 - CCNA_03031 0.70 +0.6 3,186,383 + CCNA_03031 0.75 +0.4 3,186,406 - CCNA_03031 0.78 +0.8
Or see this region's nucleotide sequence