Experiment: PYE with Dimethyl Sulfoxide 0.4 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02373 and CCNA_02374 overlap by 7 nucleotides CCNA_02374 and CCNA_02375 are separated by 58 nucleotides CCNA_02375 and CCNA_02376 overlap by 12 nucleotides CCNA_02376 and CCNA_02377 overlap by 4 nucleotides CCNA_02377 and CCNA_R0055 are separated by 225 nucleotides CCNA_R0055 and CCNA_02378 are separated by 149 nucleotides
CCNA_02373: CCNA_02373 - transposase, at 2,518,748 to 2,519,674
_02373
CCNA_02374: CCNA_02374 - transposase, at 2,519,668 to 2,519,934
_02374
CCNA_02375: CCNA_02375 - hypothetical protein, at 2,519,993 to 2,520,256
_02375
CCNA_02376: CCNA_02376 - hypothetical protein, at 2,520,245 to 2,520,523
_02376
CCNA_02377: CCNA_02377 - hypothetical protein, at 2,520,520 to 2,521,242
_02377
CCNA_R0055: CCNA_R0055 - tRNA-Ile, at 2,521,468 to 2,521,543
_R0055
CCNA_02378: CCNA_02378 - SIMPL family protein, at 2,521,693 to 2,522,439
_02378
Position (kb)
2520
2521
2522 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2519.949 kb on + strand at 2519.957 kb on + strand at 2520.068 kb on + strand, within CCNA_02375 at 2520.100 kb on - strand, within CCNA_02375 at 2520.109 kb on - strand, within CCNA_02375 at 2520.139 kb on + strand, within CCNA_02375 at 2520.147 kb on - strand, within CCNA_02375 at 2520.149 kb on + strand, within CCNA_02375 at 2520.157 kb on - strand, within CCNA_02375 at 2520.167 kb on + strand, within CCNA_02375 at 2520.167 kb on + strand, within CCNA_02375 at 2520.223 kb on + strand, within CCNA_02375 at 2520.231 kb on - strand at 2520.231 kb on - strand at 2520.231 kb on - strand at 2520.259 kb on - strand at 2520.259 kb on - strand at 2520.259 kb on - strand at 2520.259 kb on - strand at 2520.301 kb on - strand, within CCNA_02376 at 2520.323 kb on + strand, within CCNA_02376 at 2520.479 kb on - strand, within CCNA_02376 at 2520.483 kb on + strand, within CCNA_02376 at 2520.500 kb on + strand at 2520.525 kb on - strand at 2520.525 kb on - strand at 2520.600 kb on + strand, within CCNA_02377 at 2520.602 kb on - strand, within CCNA_02377 at 2520.603 kb on - strand, within CCNA_02377 at 2520.603 kb on - strand, within CCNA_02377 at 2520.651 kb on + strand, within CCNA_02377 at 2520.651 kb on + strand, within CCNA_02377 at 2520.656 kb on + strand, within CCNA_02377 at 2520.666 kb on + strand, within CCNA_02377 at 2520.708 kb on + strand, within CCNA_02377 at 2520.735 kb on - strand, within CCNA_02377 at 2520.735 kb on - strand, within CCNA_02377 at 2520.776 kb on - strand, within CCNA_02377 at 2520.901 kb on - strand, within CCNA_02377 at 2520.970 kb on - strand, within CCNA_02377 at 2520.987 kb on - strand, within CCNA_02377 at 2520.989 kb on + strand, within CCNA_02377 at 2521.043 kb on + strand, within CCNA_02377 at 2521.043 kb on + strand, within CCNA_02377 at 2521.081 kb on + strand, within CCNA_02377 at 2521.089 kb on - strand, within CCNA_02377 at 2521.104 kb on + strand, within CCNA_02377 at 2521.228 kb on + strand at 2521.302 kb on + strand at 2521.385 kb on + strand at 2521.386 kb on - strand at 2521.393 kb on - strand at 2521.587 kb on - strand at 2521.600 kb on + strand at 2521.607 kb on + strand at 2521.621 kb on - strand at 2521.621 kb on - strand at 2521.621 kb on - strand at 2521.626 kb on - strand at 2521.646 kb on - strand at 2521.673 kb on - strand at 2521.703 kb on - strand at 2521.743 kb on + strand at 2521.801 kb on + strand, within CCNA_02378 at 2521.809 kb on - strand, within CCNA_02378 at 2521.816 kb on + strand, within CCNA_02378 at 2521.824 kb on - strand, within CCNA_02378 at 2521.825 kb on - strand, within CCNA_02378 at 2521.825 kb on - strand, within CCNA_02378 at 2521.882 kb on + strand, within CCNA_02378 at 2521.890 kb on - strand, within CCNA_02378 at 2521.955 kb on + strand, within CCNA_02378 at 2521.955 kb on + strand, within CCNA_02378 at 2521.959 kb on + strand, within CCNA_02378 at 2521.963 kb on - strand, within CCNA_02378 at 2522.024 kb on + strand, within CCNA_02378 at 2522.024 kb on + strand, within CCNA_02378 at 2522.024 kb on + strand, within CCNA_02378 at 2522.050 kb on - strand, within CCNA_02378 at 2522.065 kb on + strand, within CCNA_02378 at 2522.066 kb on + strand, within CCNA_02378 at 2522.081 kb on + strand, within CCNA_02378 at 2522.123 kb on + strand, within CCNA_02378 at 2522.142 kb on + strand, within CCNA_02378 at 2522.165 kb on + strand, within CCNA_02378 at 2522.165 kb on + strand, within CCNA_02378 at 2522.165 kb on + strand, within CCNA_02378 at 2522.165 kb on + strand, within CCNA_02378 at 2522.208 kb on + strand, within CCNA_02378 at 2522.208 kb on + strand, within CCNA_02378 at 2522.216 kb on - strand, within CCNA_02378 at 2522.232 kb on + strand, within CCNA_02378 at 2522.232 kb on + strand, within CCNA_02378 at 2522.232 kb on + strand, within CCNA_02378 at 2522.240 kb on - strand, within CCNA_02378 at 2522.240 kb on - strand, within CCNA_02378
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Dimethyl Sulfoxide 0.4 % remove 2,519,949 + -2.2 2,519,957 + +0.1 2,520,068 + CCNA_02375 0.28 -1.9 2,520,100 - CCNA_02375 0.41 -0.5 2,520,109 - CCNA_02375 0.44 -0.1 2,520,139 + CCNA_02375 0.55 -0.4 2,520,147 - CCNA_02375 0.58 -1.8 2,520,149 + CCNA_02375 0.59 -1.0 2,520,157 - CCNA_02375 0.62 -1.0 2,520,167 + CCNA_02375 0.66 -0.1 2,520,167 + CCNA_02375 0.66 -0.6 2,520,223 + CCNA_02375 0.87 -0.2 2,520,231 - -0.5 2,520,231 - +0.0 2,520,231 - +0.0 2,520,259 - -0.4 2,520,259 - -0.4 2,520,259 - +0.8 2,520,259 - -0.6 2,520,301 - CCNA_02376 0.20 +0.1 2,520,323 + CCNA_02376 0.28 -1.0 2,520,479 - CCNA_02376 0.84 +0.1 2,520,483 + CCNA_02376 0.85 +0.7 2,520,500 + -1.1 2,520,525 - -1.2 2,520,525 - +0.7 2,520,600 + CCNA_02377 0.11 +0.3 2,520,602 - CCNA_02377 0.11 -0.6 2,520,603 - CCNA_02377 0.11 +0.2 2,520,603 - CCNA_02377 0.11 -0.3 2,520,651 + CCNA_02377 0.18 -1.2 2,520,651 + CCNA_02377 0.18 -1.3 2,520,656 + CCNA_02377 0.19 +0.4 2,520,666 + CCNA_02377 0.20 -1.3 2,520,708 + CCNA_02377 0.26 -0.6 2,520,735 - CCNA_02377 0.30 -0.2 2,520,735 - CCNA_02377 0.30 -0.2 2,520,776 - CCNA_02377 0.35 -0.9 2,520,901 - CCNA_02377 0.53 -0.6 2,520,970 - CCNA_02377 0.62 -1.1 2,520,987 - CCNA_02377 0.65 -2.1 2,520,989 + CCNA_02377 0.65 -1.0 2,521,043 + CCNA_02377 0.72 -1.9 2,521,043 + CCNA_02377 0.72 +0.1 2,521,081 + CCNA_02377 0.78 -1.8 2,521,089 - CCNA_02377 0.79 +0.5 2,521,104 + CCNA_02377 0.81 -0.8 2,521,228 + -0.9 2,521,302 + -3.6 2,521,385 + -0.4 2,521,386 - -0.4 2,521,393 - -0.8 2,521,587 - -1.3 2,521,600 + -0.1 2,521,607 + +0.3 2,521,621 - -0.3 2,521,621 - -1.0 2,521,621 - -0.5 2,521,626 - +0.7 2,521,646 - -1.2 2,521,673 - -1.9 2,521,703 - +0.1 2,521,743 + +0.9 2,521,801 + CCNA_02378 0.14 -1.1 2,521,809 - CCNA_02378 0.16 +0.1 2,521,816 + CCNA_02378 0.16 +0.2 2,521,824 - CCNA_02378 0.18 -0.6 2,521,825 - CCNA_02378 0.18 -1.1 2,521,825 - CCNA_02378 0.18 +0.5 2,521,882 + CCNA_02378 0.25 -0.9 2,521,890 - CCNA_02378 0.26 +0.3 2,521,955 + CCNA_02378 0.35 -0.4 2,521,955 + CCNA_02378 0.35 -1.1 2,521,959 + CCNA_02378 0.36 -0.1 2,521,963 - CCNA_02378 0.36 -0.8 2,522,024 + CCNA_02378 0.44 +2.6 2,522,024 + CCNA_02378 0.44 -0.5 2,522,024 + CCNA_02378 0.44 +0.2 2,522,050 - CCNA_02378 0.48 -0.2 2,522,065 + CCNA_02378 0.50 +0.3 2,522,066 + CCNA_02378 0.50 -0.7 2,522,081 + CCNA_02378 0.52 +0.1 2,522,123 + CCNA_02378 0.58 -0.1 2,522,142 + CCNA_02378 0.60 +0.4 2,522,165 + CCNA_02378 0.63 -1.0 2,522,165 + CCNA_02378 0.63 +0.4 2,522,165 + CCNA_02378 0.63 +0.1 2,522,165 + CCNA_02378 0.63 -0.5 2,522,208 + CCNA_02378 0.69 -1.8 2,522,208 + CCNA_02378 0.69 -0.4 2,522,216 - CCNA_02378 0.70 +0.6 2,522,232 + CCNA_02378 0.72 -0.2 2,522,232 + CCNA_02378 0.72 +0.1 2,522,232 + CCNA_02378 0.72 -0.6 2,522,240 - CCNA_02378 0.73 +2.2 2,522,240 - CCNA_02378 0.73 +0.5
Or see this region's nucleotide sequence