Experiment: PYE with Dimethyl Sulfoxide 0.4 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02250 and CCNA_02251 are separated by 60 nucleotides CCNA_02251 and CCNA_02252 are separated by 21 nucleotides CCNA_02252 and CCNA_02253 are separated by 170 nucleotides
CCNA_02250: CCNA_02250 - methylcrotonyl-CoA carboxylase biotin-containing subunit, at 2,397,843 to 2,399,807
_02250
CCNA_02251: CCNA_02251 - methylglutaconyl-CoA hydratase, at 2,399,868 to 2,400,707
_02251
CCNA_02252: CCNA_02252 - methylcrotonyl-CoA carboxylase carboxyl transferase subunit, at 2,400,729 to 2,402,321
_02252
CCNA_02253: CCNA_02253 - hybrid CAP effector domain/patatin phospholipase protein, at 2,402,492 to 2,404,267
_02253
Position (kb)
2400
2401
2402
2403 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2399.797 kb on + strand at 2399.797 kb on + strand at 2399.800 kb on - strand at 2399.805 kb on - strand at 2399.822 kb on - strand at 2399.845 kb on - strand at 2399.868 kb on - strand at 2400.125 kb on - strand, within CCNA_02251 at 2400.130 kb on - strand, within CCNA_02251 at 2400.244 kb on - strand, within CCNA_02251 at 2400.311 kb on - strand, within CCNA_02251 at 2400.311 kb on - strand, within CCNA_02251 at 2400.329 kb on - strand, within CCNA_02251 at 2400.329 kb on - strand, within CCNA_02251 at 2400.466 kb on - strand, within CCNA_02251 at 2400.533 kb on - strand, within CCNA_02251 at 2400.838 kb on + strand at 2400.838 kb on + strand at 2400.846 kb on - strand at 2400.846 kb on - strand at 2400.846 kb on - strand at 2400.910 kb on + strand, within CCNA_02252 at 2400.923 kb on - strand, within CCNA_02252 at 2401.014 kb on + strand, within CCNA_02252 at 2401.123 kb on + strand, within CCNA_02252 at 2401.123 kb on + strand, within CCNA_02252 at 2401.124 kb on - strand, within CCNA_02252 at 2401.131 kb on - strand, within CCNA_02252 at 2401.133 kb on + strand, within CCNA_02252 at 2401.162 kb on + strand, within CCNA_02252 at 2401.315 kb on + strand, within CCNA_02252 at 2401.323 kb on - strand, within CCNA_02252 at 2401.323 kb on - strand, within CCNA_02252 at 2401.323 kb on - strand, within CCNA_02252 at 2401.348 kb on + strand, within CCNA_02252 at 2401.360 kb on + strand, within CCNA_02252 at 2401.368 kb on - strand, within CCNA_02252 at 2401.383 kb on + strand, within CCNA_02252 at 2401.450 kb on + strand, within CCNA_02252 at 2401.484 kb on - strand, within CCNA_02252 at 2401.592 kb on + strand, within CCNA_02252 at 2401.604 kb on + strand, within CCNA_02252 at 2401.613 kb on - strand, within CCNA_02252 at 2401.621 kb on - strand, within CCNA_02252 at 2401.660 kb on + strand, within CCNA_02252 at 2401.660 kb on + strand, within CCNA_02252 at 2401.668 kb on - strand, within CCNA_02252 at 2401.701 kb on - strand, within CCNA_02252 at 2401.860 kb on + strand, within CCNA_02252 at 2401.894 kb on + strand, within CCNA_02252 at 2401.898 kb on + strand, within CCNA_02252 at 2401.906 kb on - strand, within CCNA_02252 at 2401.980 kb on + strand, within CCNA_02252 at 2401.988 kb on + strand, within CCNA_02252 at 2402.041 kb on + strand, within CCNA_02252 at 2402.205 kb on + strand at 2402.213 kb on - strand at 2402.213 kb on - strand at 2402.213 kb on - strand at 2402.213 kb on - strand at 2402.213 kb on - strand at 2402.213 kb on - strand at 2402.274 kb on + strand at 2402.298 kb on + strand at 2402.519 kb on + strand at 2402.554 kb on - strand at 2402.568 kb on + strand at 2402.576 kb on - strand at 2402.591 kb on - strand at 2402.594 kb on + strand at 2402.596 kb on + strand at 2402.629 kb on - strand at 2402.724 kb on + strand, within CCNA_02253 at 2402.737 kb on - strand, within CCNA_02253 at 2402.778 kb on + strand, within CCNA_02253 at 2402.786 kb on - strand, within CCNA_02253 at 2402.885 kb on + strand, within CCNA_02253 at 2402.885 kb on + strand, within CCNA_02253 at 2402.885 kb on + strand, within CCNA_02253 at 2402.885 kb on + strand, within CCNA_02253 at 2402.885 kb on + strand, within CCNA_02253 at 2402.892 kb on - strand, within CCNA_02253 at 2402.972 kb on + strand, within CCNA_02253 at 2402.972 kb on + strand, within CCNA_02253 at 2402.980 kb on - strand, within CCNA_02253 at 2403.001 kb on - strand, within CCNA_02253 at 2403.078 kb on + strand, within CCNA_02253 at 2403.086 kb on - strand, within CCNA_02253 at 2403.086 kb on - strand, within CCNA_02253 at 2403.096 kb on - strand, within CCNA_02253 at 2403.096 kb on - strand, within CCNA_02253 at 2403.096 kb on - strand, within CCNA_02253 at 2403.179 kb on - strand, within CCNA_02253 at 2403.260 kb on + strand, within CCNA_02253 at 2403.268 kb on - strand, within CCNA_02253 at 2403.268 kb on - strand, within CCNA_02253 at 2403.301 kb on + strand, within CCNA_02253
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Dimethyl Sulfoxide 0.4 % remove 2,399,797 + +0.1 2,399,797 + -1.3 2,399,800 - +0.4 2,399,805 - -1.1 2,399,822 - -0.1 2,399,845 - +1.4 2,399,868 - +0.4 2,400,125 - CCNA_02251 0.31 +0.2 2,400,130 - CCNA_02251 0.31 -1.2 2,400,244 - CCNA_02251 0.45 +2.3 2,400,311 - CCNA_02251 0.53 -0.9 2,400,311 - CCNA_02251 0.53 +1.9 2,400,329 - CCNA_02251 0.55 -1.0 2,400,329 - CCNA_02251 0.55 -0.6 2,400,466 - CCNA_02251 0.71 +1.8 2,400,533 - CCNA_02251 0.79 +1.7 2,400,838 + -0.4 2,400,838 + -0.7 2,400,846 - +0.3 2,400,846 - -2.0 2,400,846 - -0.3 2,400,910 + CCNA_02252 0.11 +2.3 2,400,923 - CCNA_02252 0.12 -0.4 2,401,014 + CCNA_02252 0.18 +1.3 2,401,123 + CCNA_02252 0.25 +0.1 2,401,123 + CCNA_02252 0.25 +0.5 2,401,124 - CCNA_02252 0.25 +3.0 2,401,131 - CCNA_02252 0.25 -0.8 2,401,133 + CCNA_02252 0.25 -1.9 2,401,162 + CCNA_02252 0.27 -1.5 2,401,315 + CCNA_02252 0.37 -1.8 2,401,323 - CCNA_02252 0.37 +0.4 2,401,323 - CCNA_02252 0.37 -1.1 2,401,323 - CCNA_02252 0.37 -0.9 2,401,348 + CCNA_02252 0.39 -1.2 2,401,360 + CCNA_02252 0.40 -0.4 2,401,368 - CCNA_02252 0.40 -1.4 2,401,383 + CCNA_02252 0.41 -0.9 2,401,450 + CCNA_02252 0.45 -1.1 2,401,484 - CCNA_02252 0.47 -0.5 2,401,592 + CCNA_02252 0.54 -1.7 2,401,604 + CCNA_02252 0.55 +0.0 2,401,613 - CCNA_02252 0.55 -1.2 2,401,621 - CCNA_02252 0.56 -0.6 2,401,660 + CCNA_02252 0.58 -1.4 2,401,660 + CCNA_02252 0.58 -0.6 2,401,668 - CCNA_02252 0.59 -0.9 2,401,701 - CCNA_02252 0.61 -0.7 2,401,860 + CCNA_02252 0.71 -0.7 2,401,894 + CCNA_02252 0.73 -1.0 2,401,898 + CCNA_02252 0.73 -1.6 2,401,906 - CCNA_02252 0.74 -1.5 2,401,980 + CCNA_02252 0.79 -1.4 2,401,988 + CCNA_02252 0.79 -0.8 2,402,041 + CCNA_02252 0.82 -2.0 2,402,205 + +0.1 2,402,213 - -1.1 2,402,213 - +0.1 2,402,213 - -1.7 2,402,213 - -1.0 2,402,213 - -0.2 2,402,213 - -0.3 2,402,274 + -2.0 2,402,298 + -1.0 2,402,519 + +0.2 2,402,554 - +0.3 2,402,568 + +1.9 2,402,576 - -0.5 2,402,591 - +1.4 2,402,594 + -0.8 2,402,596 + +0.5 2,402,629 - +0.6 2,402,724 + CCNA_02253 0.13 -0.1 2,402,737 - CCNA_02253 0.14 -0.5 2,402,778 + CCNA_02253 0.16 -0.2 2,402,786 - CCNA_02253 0.17 -2.0 2,402,885 + CCNA_02253 0.22 +1.7 2,402,885 + CCNA_02253 0.22 +0.1 2,402,885 + CCNA_02253 0.22 +0.1 2,402,885 + CCNA_02253 0.22 -0.1 2,402,885 + CCNA_02253 0.22 +0.6 2,402,892 - CCNA_02253 0.23 -0.1 2,402,972 + CCNA_02253 0.27 +1.3 2,402,972 + CCNA_02253 0.27 -0.2 2,402,980 - CCNA_02253 0.27 -0.7 2,403,001 - CCNA_02253 0.29 +0.6 2,403,078 + CCNA_02253 0.33 +0.4 2,403,086 - CCNA_02253 0.33 -0.9 2,403,086 - CCNA_02253 0.33 -0.2 2,403,096 - CCNA_02253 0.34 -0.3 2,403,096 - CCNA_02253 0.34 +0.3 2,403,096 - CCNA_02253 0.34 +1.1 2,403,179 - CCNA_02253 0.39 -0.1 2,403,260 + CCNA_02253 0.43 -0.0 2,403,268 - CCNA_02253 0.44 -0.1 2,403,268 - CCNA_02253 0.44 +0.0 2,403,301 + CCNA_02253 0.46 +0.4
Or see this region's nucleotide sequence