Experiment: PYE with Dimethyl Sulfoxide 0.4 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01966 and CCNA_01967 are separated by 71 nucleotides CCNA_01967 and CCNA_01968 are separated by 94 nucleotides CCNA_01968 and CCNA_01969 are separated by 77 nucleotides
CCNA_01966: CCNA_01966 - vitamin B12-dependent ribonucleotide reductase, at 2,108,222 to 2,110,945
_01966
CCNA_01967: CCNA_01967 - hypothetical protein with pentapeptide repeats, at 2,111,017 to 2,111,514
_01967
CCNA_01968: CCNA_01968 - pentapeptide repeats containing protein, at 2,111,609 to 2,112,478
_01968
CCNA_01969: CCNA_01969 - aspartyl-tRNA synthetase, at 2,112,556 to 2,114,394
_01969
Position (kb)
2111
2112
2113 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2110.618 kb on + strand, within CCNA_01966 at 2110.640 kb on - strand, within CCNA_01966 at 2110.642 kb on + strand, within CCNA_01966 at 2110.650 kb on - strand, within CCNA_01966 at 2110.650 kb on - strand, within CCNA_01966 at 2110.650 kb on - strand, within CCNA_01966 at 2110.650 kb on - strand, within CCNA_01966 at 2110.667 kb on + strand, within CCNA_01966 at 2110.763 kb on - strand at 2110.921 kb on + strand at 2110.934 kb on + strand at 2110.940 kb on + strand at 2110.959 kb on + strand at 2110.979 kb on + strand at 2110.979 kb on + strand at 2110.987 kb on - strand at 2111.042 kb on - strand at 2111.047 kb on - strand at 2111.056 kb on + strand at 2111.108 kb on - strand, within CCNA_01967 at 2111.108 kb on - strand, within CCNA_01967 at 2111.127 kb on - strand, within CCNA_01967 at 2111.145 kb on + strand, within CCNA_01967 at 2111.153 kb on - strand, within CCNA_01967 at 2111.153 kb on - strand, within CCNA_01967 at 2111.153 kb on - strand, within CCNA_01967 at 2111.245 kb on + strand, within CCNA_01967 at 2111.253 kb on - strand, within CCNA_01967 at 2111.271 kb on + strand, within CCNA_01967 at 2111.298 kb on - strand, within CCNA_01967 at 2111.316 kb on + strand, within CCNA_01967 at 2111.325 kb on + strand, within CCNA_01967 at 2111.348 kb on - strand, within CCNA_01967 at 2111.375 kb on + strand, within CCNA_01967 at 2111.375 kb on + strand, within CCNA_01967 at 2111.375 kb on + strand, within CCNA_01967 at 2111.410 kb on + strand, within CCNA_01967 at 2111.475 kb on - strand at 2111.476 kb on + strand at 2111.544 kb on + strand at 2111.567 kb on + strand at 2111.567 kb on + strand at 2111.600 kb on + strand at 2111.626 kb on + strand at 2111.886 kb on + strand, within CCNA_01968 at 2111.948 kb on + strand, within CCNA_01968 at 2112.436 kb on + strand at 2112.477 kb on - strand at 2112.542 kb on + strand at 2112.550 kb on - strand at 2112.550 kb on - strand at 2112.586 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Dimethyl Sulfoxide 0.4 % remove 2,110,618 + CCNA_01966 0.88 +0.9 2,110,640 - CCNA_01966 0.89 +0.4 2,110,642 + CCNA_01966 0.89 +1.1 2,110,650 - CCNA_01966 0.89 +0.1 2,110,650 - CCNA_01966 0.89 +0.8 2,110,650 - CCNA_01966 0.89 +0.5 2,110,650 - CCNA_01966 0.89 -0.3 2,110,667 + CCNA_01966 0.90 +0.1 2,110,763 - +0.1 2,110,921 + -0.0 2,110,934 + +0.1 2,110,940 + +0.8 2,110,959 + -0.5 2,110,979 + +0.1 2,110,979 + -0.9 2,110,987 - -1.4 2,111,042 - -0.3 2,111,047 - +0.9 2,111,056 + +0.5 2,111,108 - CCNA_01967 0.18 +0.4 2,111,108 - CCNA_01967 0.18 -0.4 2,111,127 - CCNA_01967 0.22 -0.3 2,111,145 + CCNA_01967 0.26 -1.3 2,111,153 - CCNA_01967 0.27 -0.9 2,111,153 - CCNA_01967 0.27 -0.6 2,111,153 - CCNA_01967 0.27 -0.4 2,111,245 + CCNA_01967 0.46 +1.7 2,111,253 - CCNA_01967 0.47 -0.9 2,111,271 + CCNA_01967 0.51 +1.6 2,111,298 - CCNA_01967 0.56 -0.0 2,111,316 + CCNA_01967 0.60 -0.2 2,111,325 + CCNA_01967 0.62 +0.3 2,111,348 - CCNA_01967 0.66 -0.5 2,111,375 + CCNA_01967 0.72 +0.4 2,111,375 + CCNA_01967 0.72 +0.4 2,111,375 + CCNA_01967 0.72 +0.4 2,111,410 + CCNA_01967 0.79 +0.7 2,111,475 - -0.7 2,111,476 + +0.6 2,111,544 + -0.2 2,111,567 + +0.4 2,111,567 + +0.2 2,111,600 + +0.1 2,111,626 + +0.4 2,111,886 + CCNA_01968 0.32 -1.9 2,111,948 + CCNA_01968 0.39 +0.1 2,112,436 + +1.9 2,112,477 - +0.2 2,112,542 + +1.1 2,112,550 - +0.1 2,112,550 - +1.8 2,112,586 - -0.5
Or see this region's nucleotide sequence