Experiment: PYE with Dimethyl Sulfoxide 0.4 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01213 and CCNA_01214 overlap by 4 nucleotides CCNA_01214 and CCNA_01215 overlap by 4 nucleotides CCNA_01215 and CCNA_01216 are separated by 105 nucleotides
CCNA_01213: CCNA_01213 - YjgP/YjgQ family membrane permease, at 1,339,720 to 1,340,781
_01213
CCNA_01214: CCNA_01214 - YjgP/YjgQ family membrane permease, at 1,340,778 to 1,341,998
_01214
CCNA_01215: CCNA_01215 - histidine triad (HIT) hydrolase, at 1,341,995 to 1,342,432
_01215
CCNA_01216: CCNA_01216 - phosphohydrolase, Icc family, at 1,342,538 to 1,343,455
_01216
Position (kb)
1340
1341
1342 Strain fitness (log2 ratio)
-2
-1
0
1 at 1339.984 kb on - strand, within CCNA_01213 at 1340.328 kb on - strand, within CCNA_01213 at 1340.451 kb on + strand, within CCNA_01213 at 1340.642 kb on - strand, within CCNA_01213 at 1340.663 kb on - strand, within CCNA_01213 at 1340.727 kb on - strand at 1340.751 kb on - strand at 1340.767 kb on + strand at 1340.770 kb on - strand at 1340.832 kb on - strand at 1340.844 kb on - strand at 1340.911 kb on - strand, within CCNA_01214 at 1340.925 kb on - strand, within CCNA_01214 at 1341.087 kb on + strand, within CCNA_01214 at 1341.721 kb on - strand, within CCNA_01214 at 1341.782 kb on + strand, within CCNA_01214 at 1341.782 kb on + strand, within CCNA_01214 at 1341.790 kb on - strand, within CCNA_01214 at 1341.790 kb on - strand, within CCNA_01214 at 1341.991 kb on - strand at 1342.075 kb on + strand, within CCNA_01215 at 1342.083 kb on - strand, within CCNA_01215 at 1342.132 kb on + strand, within CCNA_01215 at 1342.140 kb on - strand, within CCNA_01215 at 1342.140 kb on - strand, within CCNA_01215 at 1342.142 kb on + strand, within CCNA_01215 at 1342.150 kb on - strand, within CCNA_01215 at 1342.202 kb on - strand, within CCNA_01215 at 1342.239 kb on + strand, within CCNA_01215 at 1342.239 kb on + strand, within CCNA_01215 at 1342.261 kb on + strand, within CCNA_01215 at 1342.264 kb on - strand, within CCNA_01215 at 1342.303 kb on - strand, within CCNA_01215 at 1342.316 kb on - strand, within CCNA_01215 at 1342.342 kb on + strand, within CCNA_01215 at 1342.353 kb on - strand, within CCNA_01215 at 1342.355 kb on + strand, within CCNA_01215 at 1342.355 kb on + strand, within CCNA_01215 at 1342.363 kb on - strand, within CCNA_01215 at 1342.363 kb on - strand, within CCNA_01215 at 1342.368 kb on - strand, within CCNA_01215 at 1342.375 kb on - strand, within CCNA_01215 at 1342.377 kb on + strand, within CCNA_01215 at 1342.385 kb on - strand, within CCNA_01215 at 1342.387 kb on + strand, within CCNA_01215 at 1342.387 kb on + strand, within CCNA_01215 at 1342.395 kb on - strand at 1342.395 kb on - strand at 1342.395 kb on - strand at 1342.395 kb on - strand at 1342.406 kb on - strand at 1342.409 kb on + strand at 1342.417 kb on - strand at 1342.427 kb on + strand at 1342.451 kb on + strand at 1342.454 kb on - strand at 1342.454 kb on - strand at 1342.463 kb on - strand at 1342.491 kb on + strand at 1342.553 kb on - strand at 1342.558 kb on + strand at 1342.566 kb on - strand at 1342.588 kb on + strand at 1342.706 kb on + strand, within CCNA_01216 at 1342.738 kb on - strand, within CCNA_01216 at 1342.743 kb on - strand, within CCNA_01216 at 1342.743 kb on - strand, within CCNA_01216 at 1342.743 kb on - strand, within CCNA_01216 at 1342.766 kb on - strand, within CCNA_01216 at 1342.766 kb on - strand, within CCNA_01216 at 1342.781 kb on + strand, within CCNA_01216 at 1342.783 kb on + strand, within CCNA_01216 at 1342.789 kb on - strand, within CCNA_01216 at 1342.791 kb on + strand, within CCNA_01216 at 1342.797 kb on - strand, within CCNA_01216 at 1342.818 kb on - strand, within CCNA_01216 at 1342.826 kb on + strand, within CCNA_01216 at 1342.869 kb on + strand, within CCNA_01216 at 1342.877 kb on - strand, within CCNA_01216 at 1342.877 kb on - strand, within CCNA_01216 at 1342.877 kb on - strand, within CCNA_01216 at 1342.901 kb on + strand, within CCNA_01216 at 1342.901 kb on + strand, within CCNA_01216 at 1342.928 kb on + strand, within CCNA_01216 at 1342.928 kb on + strand, within CCNA_01216 at 1342.938 kb on + strand, within CCNA_01216 at 1342.946 kb on - strand, within CCNA_01216 at 1342.948 kb on - strand, within CCNA_01216 at 1342.948 kb on - strand, within CCNA_01216 at 1342.953 kb on + strand, within CCNA_01216 at 1342.961 kb on - strand, within CCNA_01216 at 1342.973 kb on + strand, within CCNA_01216 at 1342.994 kb on + strand, within CCNA_01216
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Dimethyl Sulfoxide 0.4 % remove 1,339,984 - CCNA_01213 0.25 -0.6 1,340,328 - CCNA_01213 0.57 +0.0 1,340,451 + CCNA_01213 0.69 +0.5 1,340,642 - CCNA_01213 0.87 +0.2 1,340,663 - CCNA_01213 0.89 +0.2 1,340,727 - -1.4 1,340,751 - +0.1 1,340,767 + -1.1 1,340,770 - -1.7 1,340,832 - +0.8 1,340,844 - -0.2 1,340,911 - CCNA_01214 0.11 -1.4 1,340,925 - CCNA_01214 0.12 +0.8 1,341,087 + CCNA_01214 0.25 +0.8 1,341,721 - CCNA_01214 0.77 -0.5 1,341,782 + CCNA_01214 0.82 +0.5 1,341,782 + CCNA_01214 0.82 -1.4 1,341,790 - CCNA_01214 0.83 +1.4 1,341,790 - CCNA_01214 0.83 -0.6 1,341,991 - -2.3 1,342,075 + CCNA_01215 0.18 +0.5 1,342,083 - CCNA_01215 0.20 +1.5 1,342,132 + CCNA_01215 0.31 +1.2 1,342,140 - CCNA_01215 0.33 +1.3 1,342,140 - CCNA_01215 0.33 +0.3 1,342,142 + CCNA_01215 0.34 +0.5 1,342,150 - CCNA_01215 0.35 +0.4 1,342,202 - CCNA_01215 0.47 +0.2 1,342,239 + CCNA_01215 0.56 +0.8 1,342,239 + CCNA_01215 0.56 +0.1 1,342,261 + CCNA_01215 0.61 +0.0 1,342,264 - CCNA_01215 0.61 -0.1 1,342,303 - CCNA_01215 0.70 +0.1 1,342,316 - CCNA_01215 0.73 +0.3 1,342,342 + CCNA_01215 0.79 -1.7 1,342,353 - CCNA_01215 0.82 +0.6 1,342,355 + CCNA_01215 0.82 -0.4 1,342,355 + CCNA_01215 0.82 -0.8 1,342,363 - CCNA_01215 0.84 +0.3 1,342,363 - CCNA_01215 0.84 +0.3 1,342,368 - CCNA_01215 0.85 +0.8 1,342,375 - CCNA_01215 0.87 -0.4 1,342,377 + CCNA_01215 0.87 -0.3 1,342,385 - CCNA_01215 0.89 +0.1 1,342,387 + CCNA_01215 0.89 -1.3 1,342,387 + CCNA_01215 0.89 -1.2 1,342,395 - +0.2 1,342,395 - +0.9 1,342,395 - -0.0 1,342,395 - +0.5 1,342,406 - -0.0 1,342,409 + +1.5 1,342,417 - +1.0 1,342,427 + -0.8 1,342,451 + -0.1 1,342,454 - +0.2 1,342,454 - +0.5 1,342,463 - -0.2 1,342,491 + +1.4 1,342,553 - -0.4 1,342,558 + -1.1 1,342,566 - +0.6 1,342,588 + -1.6 1,342,706 + CCNA_01216 0.18 -0.0 1,342,738 - CCNA_01216 0.22 +0.0 1,342,743 - CCNA_01216 0.22 +0.4 1,342,743 - CCNA_01216 0.22 +0.8 1,342,743 - CCNA_01216 0.22 +0.2 1,342,766 - CCNA_01216 0.25 +0.3 1,342,766 - CCNA_01216 0.25 +0.7 1,342,781 + CCNA_01216 0.26 +0.3 1,342,783 + CCNA_01216 0.27 -0.2 1,342,789 - CCNA_01216 0.27 +0.4 1,342,791 + CCNA_01216 0.28 -0.7 1,342,797 - CCNA_01216 0.28 -0.3 1,342,818 - CCNA_01216 0.31 -0.6 1,342,826 + CCNA_01216 0.31 -0.2 1,342,869 + CCNA_01216 0.36 +1.1 1,342,877 - CCNA_01216 0.37 +0.2 1,342,877 - CCNA_01216 0.37 +0.2 1,342,877 - CCNA_01216 0.37 +0.9 1,342,901 + CCNA_01216 0.40 -0.3 1,342,901 + CCNA_01216 0.40 +0.2 1,342,928 + CCNA_01216 0.42 -0.8 1,342,928 + CCNA_01216 0.42 -0.2 1,342,938 + CCNA_01216 0.44 -2.2 1,342,946 - CCNA_01216 0.44 +0.5 1,342,948 - CCNA_01216 0.45 +0.4 1,342,948 - CCNA_01216 0.45 +0.6 1,342,953 + CCNA_01216 0.45 -0.1 1,342,961 - CCNA_01216 0.46 -0.3 1,342,973 + CCNA_01216 0.47 -0.1 1,342,994 + CCNA_01216 0.50 -0.5
Or see this region's nucleotide sequence