Experiment: PYE with Dimethyl Sulfoxide 0.4 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00281 and CCNA_00282 are separated by 91 nucleotides CCNA_00282 and CCNA_00283 are separated by 117 nucleotides CCNA_00283 and CCNA_00284 overlap by 4 nucleotides
CCNA_00281: CCNA_00281 - methionyl-tRNA formyltransferase, at 293,641 to 294,567
_00281
CCNA_00282: CCNA_00282 - conserved cell surface protein, at 294,659 to 294,991
_00282
CCNA_00283: CCNA_00283 - 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase, at 295,109 to 295,969
_00283
CCNA_00284: CCNA_00284 - pyrimidine 5'-nucleotidase family protein, at 295,966 to 296,631
_00284
Position (kb) 294
295 Strain fitness (log2 ratio) -1
0
1 at 293.711 kb on + strand at 294.447 kb on + strand, within CCNA_00281 at 294.609 kb on + strand at 294.617 kb on - strand at 294.619 kb on + strand at 294.643 kb on - strand at 294.655 kb on + strand at 294.663 kb on - strand at 294.669 kb on + strand at 294.669 kb on + strand at 294.682 kb on - strand at 294.753 kb on - strand, within CCNA_00282 at 294.857 kb on - strand, within CCNA_00282 at 294.879 kb on - strand, within CCNA_00282 at 294.903 kb on + strand, within CCNA_00282 at 294.912 kb on - strand, within CCNA_00282 at 295.084 kb on - strand at 295.106 kb on - strand at 295.923 kb on + strand at 295.981 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Dimethyl Sulfoxide 0.4 % remove 293,711 + +0.7 294,447 + CCNA_00281 0.87 +0.1 294,609 + +0.3 294,617 - +0.2 294,619 + +0.4 294,643 - -0.9 294,655 + +0.4 294,663 - -0.8 294,669 + -0.0 294,669 + -0.3 294,682 - +0.8 294,753 - CCNA_00282 0.28 +0.7 294,857 - CCNA_00282 0.59 +0.2 294,879 - CCNA_00282 0.66 -0.3 294,903 + CCNA_00282 0.73 -0.8 294,912 - CCNA_00282 0.76 +0.1 295,084 - +0.6 295,106 - +0.1 295,923 + +0.1 295,981 - -0.1
Or see this region's nucleotide sequence