Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS28775 and ABID97_RS28780 are separated by 12 nucleotides ABID97_RS28780 and ABID97_RS28785 are separated by 59 nucleotides ABID97_RS28785 and ABID97_RS28790 are separated by 76 nucleotides
ABID97_RS28775: ABID97_RS28775 - branched-chain amino acid ABC transporter ATP-binding protein/permease, at 672,441 to 674,231
_RS28775
ABID97_RS28780: ABID97_RS28780 - branched-chain amino acid ABC transporter permease, at 674,244 to 675,116
_RS28780
ABID97_RS28785: ABID97_RS28785 - ABC transporter substrate-binding protein, at 675,176 to 675,985
_RS28785
ABID97_RS28790: ABID97_RS28790 - ABC transporter substrate-binding protein, at 676,062 to 676,388
_RS28790
Position (kb)
674
675
676 Strain fitness (log2 ratio)
-1
0
1
2
3 at 673.669 kb on + strand, within ABID97_RS28775 at 674.018 kb on - strand, within ABID97_RS28775 at 674.033 kb on - strand, within ABID97_RS28775 at 674.201 kb on - strand at 674.246 kb on - strand at 674.246 kb on - strand at 674.317 kb on + strand at 674.318 kb on - strand at 674.318 kb on - strand at 674.347 kb on + strand, within ABID97_RS28780 at 674.459 kb on - strand, within ABID97_RS28780 at 674.552 kb on - strand, within ABID97_RS28780 at 674.552 kb on - strand, within ABID97_RS28780 at 674.932 kb on + strand, within ABID97_RS28780 at 674.943 kb on - strand, within ABID97_RS28780 at 675.064 kb on + strand at 676.030 kb on + strand at 676.087 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 673,669 + ABID97_RS28775 0.69 +0.2 674,018 - ABID97_RS28775 0.88 +0.1 674,033 - ABID97_RS28775 0.89 -0.6 674,201 - +2.8 674,246 - -0.1 674,246 - +0.2 674,317 + -0.3 674,318 - -0.2 674,318 - -0.2 674,347 + ABID97_RS28780 0.12 -0.0 674,459 - ABID97_RS28780 0.25 +0.5 674,552 - ABID97_RS28780 0.35 -0.0 674,552 - ABID97_RS28780 0.35 +1.3 674,932 + ABID97_RS28780 0.79 -0.2 674,943 - ABID97_RS28780 0.80 +0.2 675,064 + -0.1 676,030 + +1.2 676,087 + -1.2
Or see this region's nucleotide sequence