Strain Fitness in Variovorax sp. OAS795 around ABID97_RS20545

Experiment: L-Threonine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABID97_RS20535 and ABID97_RS20540 are separated by 121 nucleotidesABID97_RS20540 and ABID97_RS20545 are separated by 190 nucleotidesABID97_RS20545 and ABID97_RS20550 overlap by 65 nucleotides ABID97_RS20535: ABID97_RS20535 - branched-chain amino acid ABC transporter substrate-binding protein, at 4,238,224 to 4,239,504 _RS20535 ABID97_RS20540: ABID97_RS20540 - Hsp20/alpha crystallin family protein, at 4,239,626 to 4,239,997 _RS20540 ABID97_RS20545: ABID97_RS20545 - alpha/beta hydrolase-fold protein, at 4,240,188 to 4,240,946 _RS20545 ABID97_RS20550: ABID97_RS20550 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 4,240,882 to 4,241,943 _RS20550 Position (kb) 4240 4241Strain fitness (log2 ratio) -1 0 1 2at 4239.251 kb on + strand, within ABID97_RS20535at 4239.531 kb on + strandat 4239.531 kb on + strandat 4239.532 kb on - strandat 4239.532 kb on - strandat 4239.578 kb on - strandat 4240.061 kb on + strandat 4240.071 kb on - strandat 4240.260 kb on + strandat 4240.359 kb on + strand, within ABID97_RS20545at 4240.359 kb on + strand, within ABID97_RS20545at 4240.365 kb on + strand, within ABID97_RS20545at 4240.365 kb on + strand, within ABID97_RS20545at 4240.365 kb on + strand, within ABID97_RS20545at 4240.366 kb on - strand, within ABID97_RS20545at 4240.366 kb on - strand, within ABID97_RS20545at 4240.428 kb on + strand, within ABID97_RS20545at 4240.428 kb on + strand, within ABID97_RS20545at 4240.884 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Threonine (C)
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4,239,251 + ABID97_RS20535 0.80 +2.0
4,239,531 + -0.2
4,239,531 + +0.2
4,239,532 - +0.6
4,239,532 - +0.4
4,239,578 - -0.4
4,240,061 + +1.3
4,240,071 - +1.1
4,240,260 + +0.4
4,240,359 + ABID97_RS20545 0.23 +1.1
4,240,359 + ABID97_RS20545 0.23 +0.0
4,240,365 + ABID97_RS20545 0.23 +0.3
4,240,365 + ABID97_RS20545 0.23 -0.6
4,240,365 + ABID97_RS20545 0.23 +2.0
4,240,366 - ABID97_RS20545 0.23 +0.3
4,240,366 - ABID97_RS20545 0.23 -0.8
4,240,428 + ABID97_RS20545 0.32 -0.3
4,240,428 + ABID97_RS20545 0.32 +0.0
4,240,884 - +0.2

Or see this region's nucleotide sequence