Experiment: m.b. Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0005 and Echvi_0006 are separated by 15 nucleotides Echvi_0006 and Echvi_0007 overlap by 20 nucleotides Echvi_0007 and Echvi_0008 are separated by 13 nucleotides Echvi_0008 and Echvi_0009 are separated by 291 nucleotides
Echvi_0005: Echvi_0005 - Predicted membrane protein, at 4,135 to 4,671
_0005
Echvi_0006: Echvi_0006 - Uncharacterized protein SCO1/SenC/PrrC, involved in biogenesis of respiratory and photosynthetic systems, at 4,687 to 5,355
_0006
Echvi_0007: Echvi_0007 - Cytochrome c, mono- and diheme variants, at 5,336 to 5,800
_0007
Echvi_0008: Echvi_0008 - hypothetical protein, at 5,814 to 6,212
_0008
Echvi_0009: Echvi_0009 - Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase, at 6,504 to 7,325
_0009
Position (kb) 5
6 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4.677 kb on + strand at 4.709 kb on + strand at 4.709 kb on + strand at 4.709 kb on + strand at 4.709 kb on + strand at 4.710 kb on - strand at 4.713 kb on + strand at 4.891 kb on - strand, within Echvi_0006 at 4.922 kb on + strand, within Echvi_0006 at 4.922 kb on + strand, within Echvi_0006 at 4.922 kb on + strand, within Echvi_0006 at 4.923 kb on - strand, within Echvi_0006 at 4.924 kb on + strand, within Echvi_0006 at 4.925 kb on - strand, within Echvi_0006 at 4.945 kb on - strand, within Echvi_0006 at 4.996 kb on + strand, within Echvi_0006 at 5.033 kb on + strand, within Echvi_0006 at 5.061 kb on + strand, within Echvi_0006 at 5.076 kb on - strand, within Echvi_0006 at 5.259 kb on + strand at 5.260 kb on - strand, within Echvi_0006 at 5.273 kb on - strand, within Echvi_0006 at 5.434 kb on + strand, within Echvi_0007 at 5.475 kb on - strand, within Echvi_0007 at 5.496 kb on + strand, within Echvi_0007 at 5.496 kb on + strand, within Echvi_0007 at 5.496 kb on + strand, within Echvi_0007 at 5.496 kb on + strand, within Echvi_0007 at 5.497 kb on - strand, within Echvi_0007 at 5.497 kb on - strand, within Echvi_0007 at 5.508 kb on - strand, within Echvi_0007 at 5.508 kb on - strand, within Echvi_0007 at 5.661 kb on - strand, within Echvi_0007 at 5.706 kb on + strand, within Echvi_0007 at 5.724 kb on - strand, within Echvi_0007 at 5.724 kb on - strand, within Echvi_0007 at 5.730 kb on - strand, within Echvi_0007 at 5.799 kb on - strand at 5.802 kb on + strand at 5.818 kb on + strand at 5.818 kb on + strand at 5.894 kb on - strand, within Echvi_0008 at 5.936 kb on + strand, within Echvi_0008 at 5.937 kb on - strand, within Echvi_0008 at 5.937 kb on - strand, within Echvi_0008 at 5.938 kb on + strand, within Echvi_0008 at 6.014 kb on + strand, within Echvi_0008 at 6.087 kb on + strand, within Echvi_0008 at 6.242 kb on + strand at 6.242 kb on + strand at 6.242 kb on + strand at 6.243 kb on - strand at 6.243 kb on - strand at 6.243 kb on - strand at 6.243 kb on - strand at 6.244 kb on + strand at 6.245 kb on - strand at 6.416 kb on + strand at 6.417 kb on - strand at 6.482 kb on - strand at 6.488 kb on - strand at 6.488 kb on - strand at 6.488 kb on - strand at 6.496 kb on + strand at 6.497 kb on - strand at 6.514 kb on - strand at 6.533 kb on + strand at 6.533 kb on + strand at 6.635 kb on - strand, within Echvi_0009 at 6.641 kb on - strand, within Echvi_0009 at 6.648 kb on + strand, within Echvi_0009 at 6.649 kb on - strand, within Echvi_0009 at 6.716 kb on + strand, within Echvi_0009 at 6.728 kb on - strand, within Echvi_0009 at 6.737 kb on + strand, within Echvi_0009 at 6.737 kb on + strand, within Echvi_0009 at 6.737 kb on + strand, within Echvi_0009
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.05 mg/ml remove 4,677 + +1.3 4,709 + -0.4 4,709 + +2.6 4,709 + +0.5 4,709 + -0.0 4,710 - -0.1 4,713 + +1.1 4,891 - Echvi_0006 0.30 +1.6 4,922 + Echvi_0006 0.35 -2.3 4,922 + Echvi_0006 0.35 +0.4 4,922 + Echvi_0006 0.35 -1.1 4,923 - Echvi_0006 0.35 +1.7 4,924 + Echvi_0006 0.35 +1.3 4,925 - Echvi_0006 0.36 +0.4 4,945 - Echvi_0006 0.39 +1.7 4,996 + Echvi_0006 0.46 +1.2 5,033 + Echvi_0006 0.52 +0.9 5,061 + Echvi_0006 0.56 +1.0 5,076 - Echvi_0006 0.58 -0.0 5,259 + +1.6 5,260 - Echvi_0006 0.86 +0.5 5,273 - Echvi_0006 0.88 -0.0 5,434 + Echvi_0007 0.21 +0.1 5,475 - Echvi_0007 0.30 +1.0 5,496 + Echvi_0007 0.34 +1.8 5,496 + Echvi_0007 0.34 +1.0 5,496 + Echvi_0007 0.34 +0.5 5,496 + Echvi_0007 0.34 +1.7 5,497 - Echvi_0007 0.35 +1.2 5,497 - Echvi_0007 0.35 +1.0 5,508 - Echvi_0007 0.37 +2.4 5,508 - Echvi_0007 0.37 +3.0 5,661 - Echvi_0007 0.70 +1.6 5,706 + Echvi_0007 0.80 +1.1 5,724 - Echvi_0007 0.83 +0.4 5,724 - Echvi_0007 0.83 +2.8 5,730 - Echvi_0007 0.85 +1.9 5,799 - -0.1 5,802 + -0.5 5,818 + +1.6 5,818 + +1.1 5,894 - Echvi_0008 0.20 +1.0 5,936 + Echvi_0008 0.31 -0.9 5,937 - Echvi_0008 0.31 -0.9 5,937 - Echvi_0008 0.31 +0.4 5,938 + Echvi_0008 0.31 +1.1 6,014 + Echvi_0008 0.50 +0.6 6,087 + Echvi_0008 0.68 +0.6 6,242 + +0.0 6,242 + +0.8 6,242 + +1.0 6,243 - -1.5 6,243 - +0.7 6,243 - +1.0 6,243 - -0.6 6,244 + -0.1 6,245 - -0.3 6,416 + -0.2 6,417 - +0.5 6,482 - +0.6 6,488 - +0.3 6,488 - -0.3 6,488 - +0.8 6,496 + +2.0 6,497 - -0.6 6,514 - -1.4 6,533 + +0.0 6,533 + +0.2 6,635 - Echvi_0009 0.16 +0.1 6,641 - Echvi_0009 0.17 +0.0 6,648 + Echvi_0009 0.18 -0.2 6,649 - Echvi_0009 0.18 -0.3 6,716 + Echvi_0009 0.26 +1.7 6,728 - Echvi_0009 0.27 -1.8 6,737 + Echvi_0009 0.28 +0.4 6,737 + Echvi_0009 0.28 +1.2 6,737 + Echvi_0009 0.28 -0.1
Or see this region's nucleotide sequence