Experiment: MS_media no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3894 and EX28DRAFT_3895 are separated by 205 nucleotides EX28DRAFT_3895 and EX28DRAFT_3896 are separated by 2 nucleotides EX28DRAFT_3896 and EX28DRAFT_3897 are separated by 251 nucleotides EX28DRAFT_3897 and EX28DRAFT_3898 are separated by 54 nucleotides
EX28DRAFT_3894: EX28DRAFT_3894 - TIGR00255 family protein, at 101,533 to 102,396
_3894
EX28DRAFT_3895: EX28DRAFT_3895 - Predicted membrane protein, at 102,602 to 103,219
_3895
EX28DRAFT_3896: EX28DRAFT_3896 - NAD-dependent DNA ligase (contains BRCT domain type II), at 103,222 to 104,892
_3896
EX28DRAFT_3897: EX28DRAFT_3897 - guanylate kinase (EC 2.7.4.8), at 105,144 to 105,767
_3897
EX28DRAFT_3898: EX28DRAFT_3898 - DNA-directed RNA polymerase subunit omega (EC 2.7.7.6), at 105,822 to 106,097
_3898
Position (kb)
103
104
105 Strain fitness (log2 ratio)
-1
0
1 at 102.264 kb on + strand, within EX28DRAFT_3894 at 102.264 kb on + strand, within EX28DRAFT_3894 at 102.264 kb on + strand, within EX28DRAFT_3894 at 102.599 kb on + strand at 102.599 kb on + strand at 102.601 kb on + strand at 102.601 kb on + strand at 102.602 kb on - strand at 102.602 kb on - strand at 102.602 kb on - strand at 102.709 kb on + strand, within EX28DRAFT_3895 at 102.792 kb on - strand, within EX28DRAFT_3895 at 102.800 kb on - strand, within EX28DRAFT_3895 at 103.003 kb on + strand, within EX28DRAFT_3895 at 103.004 kb on - strand, within EX28DRAFT_3895 at 103.046 kb on + strand, within EX28DRAFT_3895 at 103.079 kb on + strand, within EX28DRAFT_3895 at 103.079 kb on + strand, within EX28DRAFT_3895 at 103.080 kb on - strand, within EX28DRAFT_3895 at 103.080 kb on - strand, within EX28DRAFT_3895 at 103.082 kb on - strand, within EX28DRAFT_3895 at 103.082 kb on - strand, within EX28DRAFT_3895 at 103.097 kb on + strand, within EX28DRAFT_3895 at 103.120 kb on - strand, within EX28DRAFT_3895 at 103.217 kb on + strand at 103.378 kb on + strand at 103.379 kb on - strand at 103.467 kb on + strand, within EX28DRAFT_3896 at 103.468 kb on - strand, within EX28DRAFT_3896 at 103.468 kb on - strand, within EX28DRAFT_3896 at 103.516 kb on - strand, within EX28DRAFT_3896 at 103.540 kb on + strand, within EX28DRAFT_3896 at 103.541 kb on - strand, within EX28DRAFT_3896 at 103.710 kb on - strand, within EX28DRAFT_3896 at 103.734 kb on + strand, within EX28DRAFT_3896 at 103.734 kb on + strand, within EX28DRAFT_3896 at 103.735 kb on - strand, within EX28DRAFT_3896 at 103.735 kb on - strand, within EX28DRAFT_3896 at 103.764 kb on + strand, within EX28DRAFT_3896 at 103.830 kb on + strand, within EX28DRAFT_3896 at 103.878 kb on + strand, within EX28DRAFT_3896 at 103.909 kb on + strand, within EX28DRAFT_3896 at 103.909 kb on + strand, within EX28DRAFT_3896 at 103.910 kb on - strand, within EX28DRAFT_3896 at 104.199 kb on + strand, within EX28DRAFT_3896 at 104.199 kb on + strand, within EX28DRAFT_3896 at 104.199 kb on + strand, within EX28DRAFT_3896 at 104.199 kb on + strand, within EX28DRAFT_3896 at 104.200 kb on - strand, within EX28DRAFT_3896 at 104.206 kb on - strand, within EX28DRAFT_3896 at 104.206 kb on - strand, within EX28DRAFT_3896 at 104.257 kb on + strand, within EX28DRAFT_3896 at 104.369 kb on - strand, within EX28DRAFT_3896 at 104.420 kb on - strand, within EX28DRAFT_3896 at 104.425 kb on + strand, within EX28DRAFT_3896 at 104.425 kb on + strand, within EX28DRAFT_3896 at 104.473 kb on - strand, within EX28DRAFT_3896 at 104.473 kb on - strand, within EX28DRAFT_3896 at 104.487 kb on + strand, within EX28DRAFT_3896 at 104.488 kb on - strand, within EX28DRAFT_3896 at 104.626 kb on + strand, within EX28DRAFT_3896 at 104.626 kb on + strand, within EX28DRAFT_3896 at 104.908 kb on + strand at 104.922 kb on - strand at 105.012 kb on - strand at 105.071 kb on - strand at 105.097 kb on + strand at 105.097 kb on + strand at 105.105 kb on + strand at 105.817 kb on + strand at 105.821 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media no stress control remove 102,264 + EX28DRAFT_3894 0.85 +0.3 102,264 + EX28DRAFT_3894 0.85 +0.0 102,264 + EX28DRAFT_3894 0.85 -0.1 102,599 + -0.6 102,599 + -0.2 102,601 + +0.1 102,601 + -0.5 102,602 - +0.2 102,602 - +0.4 102,602 - +0.1 102,709 + EX28DRAFT_3895 0.17 -0.6 102,792 - EX28DRAFT_3895 0.31 -0.4 102,800 - EX28DRAFT_3895 0.32 +0.5 103,003 + EX28DRAFT_3895 0.65 -1.0 103,004 - EX28DRAFT_3895 0.65 -0.4 103,046 + EX28DRAFT_3895 0.72 +0.5 103,079 + EX28DRAFT_3895 0.77 +0.7 103,079 + EX28DRAFT_3895 0.77 -0.1 103,080 - EX28DRAFT_3895 0.77 +0.3 103,080 - EX28DRAFT_3895 0.77 +0.6 103,082 - EX28DRAFT_3895 0.78 +0.2 103,082 - EX28DRAFT_3895 0.78 +0.5 103,097 + EX28DRAFT_3895 0.80 -0.2 103,120 - EX28DRAFT_3895 0.84 +0.7 103,217 + +0.1 103,378 + -0.1 103,379 - -0.1 103,467 + EX28DRAFT_3896 0.15 -0.0 103,468 - EX28DRAFT_3896 0.15 -0.3 103,468 - EX28DRAFT_3896 0.15 +0.2 103,516 - EX28DRAFT_3896 0.18 +0.1 103,540 + EX28DRAFT_3896 0.19 +0.3 103,541 - EX28DRAFT_3896 0.19 -0.0 103,710 - EX28DRAFT_3896 0.29 +0.2 103,734 + EX28DRAFT_3896 0.31 -0.1 103,734 + EX28DRAFT_3896 0.31 -0.4 103,735 - EX28DRAFT_3896 0.31 -0.1 103,735 - EX28DRAFT_3896 0.31 -0.1 103,764 + EX28DRAFT_3896 0.32 -0.1 103,830 + EX28DRAFT_3896 0.36 -0.5 103,878 + EX28DRAFT_3896 0.39 +0.4 103,909 + EX28DRAFT_3896 0.41 +0.3 103,909 + EX28DRAFT_3896 0.41 -0.6 103,910 - EX28DRAFT_3896 0.41 +0.2 104,199 + EX28DRAFT_3896 0.58 -0.3 104,199 + EX28DRAFT_3896 0.58 +0.1 104,199 + EX28DRAFT_3896 0.58 +0.6 104,199 + EX28DRAFT_3896 0.58 -0.5 104,200 - EX28DRAFT_3896 0.59 -0.1 104,206 - EX28DRAFT_3896 0.59 +1.3 104,206 - EX28DRAFT_3896 0.59 -0.1 104,257 + EX28DRAFT_3896 0.62 +0.2 104,369 - EX28DRAFT_3896 0.69 +0.3 104,420 - EX28DRAFT_3896 0.72 +0.3 104,425 + EX28DRAFT_3896 0.72 +0.4 104,425 + EX28DRAFT_3896 0.72 -0.2 104,473 - EX28DRAFT_3896 0.75 +0.4 104,473 - EX28DRAFT_3896 0.75 +0.1 104,487 + EX28DRAFT_3896 0.76 +0.4 104,488 - EX28DRAFT_3896 0.76 +0.2 104,626 + EX28DRAFT_3896 0.84 +0.3 104,626 + EX28DRAFT_3896 0.84 +1.2 104,908 + +0.0 104,922 - +0.1 105,012 - +0.3 105,071 - +0.3 105,097 + -1.0 105,097 + -0.6 105,105 + -1.0 105,817 + -0.5 105,821 + -0.1
Or see this region's nucleotide sequence