Experiment: MS_media no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3287 and EX28DRAFT_3288 are separated by 111 nucleotides EX28DRAFT_3288 and EX28DRAFT_3289 are separated by 62 nucleotides EX28DRAFT_3289 and EX28DRAFT_3290 are separated by 45 nucleotides EX28DRAFT_3290 and EX28DRAFT_3291 are separated by 90 nucleotides
EX28DRAFT_3287: EX28DRAFT_3287 - Protein of unknown function (DUF1471), at 584,961 to 585,224
_3287
EX28DRAFT_3288: EX28DRAFT_3288 - Protein of unknown function (DUF1471), at 585,336 to 585,602
_3288
EX28DRAFT_3289: EX28DRAFT_3289 - Barstar, RNAse (barnase) inhibitor, at 585,665 to 585,937
_3289
EX28DRAFT_3290: EX28DRAFT_3290 - succinate-semialdehyde dehydrogenase, at 585,983 to 587,437
_3290
EX28DRAFT_3291: EX28DRAFT_3291 - Predicted membrane protein, at 587,528 to 589,495
_3291
Position (kb) 585
586
587
588 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 585.035 kb on + strand, within EX28DRAFT_3287 at 585.217 kb on - strand at 585.230 kb on - strand at 585.231 kb on - strand at 585.318 kb on + strand at 585.329 kb on - strand at 585.329 kb on - strand at 585.335 kb on + strand at 585.416 kb on - strand, within EX28DRAFT_3288 at 585.439 kb on + strand, within EX28DRAFT_3288 at 585.446 kb on + strand, within EX28DRAFT_3288 at 585.447 kb on - strand, within EX28DRAFT_3288 at 585.447 kb on - strand, within EX28DRAFT_3288 at 585.596 kb on + strand at 585.597 kb on - strand at 585.597 kb on - strand at 585.601 kb on - strand at 585.601 kb on - strand at 585.601 kb on - strand at 585.623 kb on + strand at 585.624 kb on - strand at 585.925 kb on + strand at 585.925 kb on + strand at 585.925 kb on + strand at 585.926 kb on - strand at 585.926 kb on - strand at 585.927 kb on + strand at 585.927 kb on + strand at 585.928 kb on - strand at 585.984 kb on + strand at 585.984 kb on + strand at 585.985 kb on - strand at 585.985 kb on - strand at 585.985 kb on - strand at 585.986 kb on + strand at 585.997 kb on + strand at 585.997 kb on + strand at 586.020 kb on + strand at 586.020 kb on + strand at 586.021 kb on - strand at 586.071 kb on - strand at 586.185 kb on + strand, within EX28DRAFT_3290 at 586.185 kb on + strand, within EX28DRAFT_3290 at 586.320 kb on - strand, within EX28DRAFT_3290 at 586.513 kb on + strand, within EX28DRAFT_3290 at 586.651 kb on - strand, within EX28DRAFT_3290 at 586.753 kb on + strand, within EX28DRAFT_3290 at 586.798 kb on + strand, within EX28DRAFT_3290 at 586.798 kb on + strand, within EX28DRAFT_3290 at 586.799 kb on - strand, within EX28DRAFT_3290 at 586.799 kb on - strand, within EX28DRAFT_3290 at 586.855 kb on + strand, within EX28DRAFT_3290 at 586.856 kb on - strand, within EX28DRAFT_3290 at 587.197 kb on - strand, within EX28DRAFT_3290 at 587.197 kb on - strand, within EX28DRAFT_3290 at 587.275 kb on + strand, within EX28DRAFT_3290 at 587.314 kb on + strand at 587.315 kb on - strand at 587.360 kb on - strand at 587.460 kb on - strand at 587.460 kb on - strand at 587.476 kb on - strand at 587.476 kb on - strand at 587.479 kb on + strand at 587.479 kb on + strand at 587.480 kb on - strand at 587.480 kb on - strand at 587.480 kb on - strand at 587.480 kb on - strand at 587.480 kb on - strand at 587.482 kb on - strand at 587.520 kb on + strand at 587.566 kb on + strand at 587.634 kb on + strand at 587.663 kb on - strand at 587.668 kb on - strand at 587.668 kb on - strand at 587.868 kb on + strand, within EX28DRAFT_3291 at 587.869 kb on - strand, within EX28DRAFT_3291 at 587.912 kb on - strand, within EX28DRAFT_3291 at 587.996 kb on + strand, within EX28DRAFT_3291 at 588.181 kb on + strand, within EX28DRAFT_3291 at 588.181 kb on + strand, within EX28DRAFT_3291 at 588.234 kb on + strand, within EX28DRAFT_3291 at 588.293 kb on - strand, within EX28DRAFT_3291
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media no stress control remove 585,035 + EX28DRAFT_3287 0.28 -0.2 585,217 - -0.6 585,230 - +0.1 585,231 - -0.2 585,318 + -2.9 585,329 - -2.3 585,329 - +0.3 585,335 + -0.1 585,416 - EX28DRAFT_3288 0.30 +0.5 585,439 + EX28DRAFT_3288 0.39 +0.1 585,446 + EX28DRAFT_3288 0.41 +0.5 585,447 - EX28DRAFT_3288 0.42 +0.3 585,447 - EX28DRAFT_3288 0.42 +0.2 585,596 + -0.5 585,597 - +0.2 585,597 - +1.0 585,601 - +0.5 585,601 - +0.5 585,601 - -1.1 585,623 + -0.4 585,624 - +0.3 585,925 + +0.4 585,925 + +0.3 585,925 + +0.1 585,926 - -0.5 585,926 - +0.2 585,927 + +0.1 585,927 + +0.1 585,928 - -0.1 585,984 + +0.1 585,984 + +0.2 585,985 - -0.6 585,985 - +0.3 585,985 - +0.8 585,986 + -0.4 585,997 + -1.1 585,997 + +0.5 586,020 + +0.5 586,020 + -0.6 586,021 - +0.2 586,071 - +0.2 586,185 + EX28DRAFT_3290 0.14 +0.6 586,185 + EX28DRAFT_3290 0.14 +0.0 586,320 - EX28DRAFT_3290 0.23 +1.0 586,513 + EX28DRAFT_3290 0.36 -0.8 586,651 - EX28DRAFT_3290 0.46 -0.8 586,753 + EX28DRAFT_3290 0.53 +0.4 586,798 + EX28DRAFT_3290 0.56 -0.7 586,798 + EX28DRAFT_3290 0.56 +0.1 586,799 - EX28DRAFT_3290 0.56 -0.8 586,799 - EX28DRAFT_3290 0.56 +0.1 586,855 + EX28DRAFT_3290 0.60 -0.2 586,856 - EX28DRAFT_3290 0.60 -1.0 587,197 - EX28DRAFT_3290 0.83 -0.3 587,197 - EX28DRAFT_3290 0.83 -0.7 587,275 + EX28DRAFT_3290 0.89 +0.2 587,314 + +0.1 587,315 - +0.1 587,360 - -0.2 587,460 - +0.4 587,460 - -0.2 587,476 - +0.7 587,476 - -4.6 587,479 + -0.2 587,479 + -0.2 587,480 - -0.5 587,480 - -0.8 587,480 - +1.1 587,480 - +0.5 587,480 - +0.0 587,482 - +0.3 587,520 + +0.3 587,566 + -0.0 587,634 + +0.2 587,663 - +0.5 587,668 - +0.6 587,668 - -0.1 587,868 + EX28DRAFT_3291 0.17 +0.0 587,869 - EX28DRAFT_3291 0.17 +0.1 587,912 - EX28DRAFT_3291 0.20 +0.3 587,996 + EX28DRAFT_3291 0.24 -1.3 588,181 + EX28DRAFT_3291 0.33 +0.1 588,181 + EX28DRAFT_3291 0.33 -0.0 588,234 + EX28DRAFT_3291 0.36 -1.3 588,293 - EX28DRAFT_3291 0.39 -0.1
Or see this region's nucleotide sequence