Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03702 and CCNA_03703 are separated by 148 nucleotides CCNA_03703 and CCNA_03704 are separated by 114 nucleotides CCNA_03704 and CCNA_03705 are separated by 118 nucleotides CCNA_03705 and CCNA_R0089 are separated by 215 nucleotides
CCNA_03702: CCNA_03702 - SSU ribosomal protein S1P, at 3,869,691 to 3,871,400
_03702
CCNA_03703: CCNA_03703 - cytidylate kinase, at 3,871,549 to 3,872,190
_03703
CCNA_03704: CCNA_03704 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 3,872,305 to 3,873,636
_03704
CCNA_03705: CCNA_03705 - hypothetical protein, at 3,873,755 to 3,874,225
_03705
CCNA_R0089: CCNA_R0089 - tRNA-Ala, at 3,874,441 to 3,874,516
_R0089
Position (kb)
3872
3873
3874 Strain fitness (log2 ratio)
-2
-1
0
1 at 3872.186 kb on + strand at 3872.186 kb on + strand at 3872.217 kb on - strand at 3872.225 kb on + strand at 3872.226 kb on + strand at 3872.231 kb on + strand at 3872.233 kb on - strand at 3872.234 kb on - strand at 3872.234 kb on - strand at 3872.234 kb on - strand at 3872.239 kb on - strand at 3872.239 kb on - strand at 3872.253 kb on - strand at 3872.273 kb on - strand at 3872.273 kb on - strand at 3872.273 kb on - strand at 3872.281 kb on + strand at 3872.281 kb on + strand at 3872.281 kb on + strand at 3872.289 kb on - strand at 3872.301 kb on - strand at 3872.303 kb on + strand at 3872.303 kb on + strand at 3872.303 kb on + strand at 3872.303 kb on + strand at 3872.311 kb on - strand at 3872.317 kb on - strand at 3872.319 kb on + strand at 3872.325 kb on + strand at 3872.346 kb on - strand at 3873.317 kb on - strand, within CCNA_03704 at 3873.616 kb on + strand at 3873.616 kb on + strand at 3873.624 kb on - strand at 3873.650 kb on + strand at 3873.658 kb on - strand at 3873.787 kb on - strand at 3873.851 kb on + strand, within CCNA_03705 at 3873.883 kb on - strand, within CCNA_03705 at 3873.951 kb on + strand, within CCNA_03705 at 3874.109 kb on + strand, within CCNA_03705 at 3874.117 kb on - strand, within CCNA_03705 at 3874.273 kb on - strand at 3874.584 kb on - strand at 3874.584 kb on - strand at 3874.613 kb on - strand at 3874.615 kb on + strand at 3874.623 kb on - strand at 3874.623 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 3,872,186 + -0.2 3,872,186 + +0.5 3,872,217 - -0.3 3,872,225 + +0.6 3,872,226 + +0.4 3,872,231 + -1.5 3,872,233 - -2.3 3,872,234 - -0.0 3,872,234 - +0.2 3,872,234 - +0.6 3,872,239 - +0.4 3,872,239 - +0.1 3,872,253 - +0.1 3,872,273 - -0.1 3,872,273 - +0.4 3,872,273 - -0.1 3,872,281 + +0.1 3,872,281 + -1.0 3,872,281 + -0.3 3,872,289 - -0.9 3,872,301 - -0.3 3,872,303 + -0.1 3,872,303 + -0.0 3,872,303 + -0.7 3,872,303 + -0.3 3,872,311 - -0.1 3,872,317 - +0.3 3,872,319 + -1.9 3,872,325 + -0.2 3,872,346 - +0.2 3,873,317 - CCNA_03704 0.76 -0.8 3,873,616 + +1.1 3,873,616 + -0.1 3,873,624 - +0.3 3,873,650 + +0.1 3,873,658 - -0.4 3,873,787 - +0.6 3,873,851 + CCNA_03705 0.20 +1.1 3,873,883 - CCNA_03705 0.27 +0.9 3,873,951 + CCNA_03705 0.42 -0.9 3,874,109 + CCNA_03705 0.75 +0.1 3,874,117 - CCNA_03705 0.77 +0.1 3,874,273 - -0.1 3,874,584 - -2.0 3,874,584 - +0.2 3,874,613 - +1.0 3,874,615 + -0.5 3,874,623 - -0.4 3,874,623 - -1.0
Or see this region's nucleotide sequence