Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02115 and CCNA_02116 are separated by 127 nucleotides CCNA_02116 and CCNA_02117 are separated by 114 nucleotides CCNA_02117 and CCNA_02118 are separated by 82 nucleotides
CCNA_02115: CCNA_02115 - secreted pectate lyase-family protein, at 2,263,969 to 2,265,600
_02115
CCNA_02116: CCNA_02116 - hypothetical protein, at 2,265,728 to 2,266,525
_02116
CCNA_02117: CCNA_02117 - polyketide cyclase/lipid transport protein, at 2,266,640 to 2,267,236
_02117
CCNA_02118: CCNA_02118 - ATP-dependent helicase, dinG family, at 2,267,319 to 2,269,502
_02118
Position (kb)
2265
2266
2267 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2264.728 kb on + strand, within CCNA_02115 at 2264.777 kb on + strand, within CCNA_02115 at 2264.777 kb on + strand, within CCNA_02115 at 2264.804 kb on - strand, within CCNA_02115 at 2264.804 kb on - strand, within CCNA_02115 at 2264.821 kb on + strand, within CCNA_02115 at 2264.842 kb on + strand, within CCNA_02115 at 2264.848 kb on - strand, within CCNA_02115 at 2264.865 kb on - strand, within CCNA_02115 at 2264.872 kb on + strand, within CCNA_02115 at 2264.885 kb on + strand, within CCNA_02115 at 2264.923 kb on + strand, within CCNA_02115 at 2264.935 kb on + strand, within CCNA_02115 at 2264.974 kb on + strand, within CCNA_02115 at 2264.982 kb on - strand, within CCNA_02115 at 2264.982 kb on - strand, within CCNA_02115 at 2264.993 kb on + strand, within CCNA_02115 at 2264.993 kb on + strand, within CCNA_02115 at 2265.001 kb on - strand, within CCNA_02115 at 2265.021 kb on - strand, within CCNA_02115 at 2265.039 kb on - strand, within CCNA_02115 at 2265.071 kb on + strand, within CCNA_02115 at 2265.081 kb on - strand, within CCNA_02115 at 2265.108 kb on + strand, within CCNA_02115 at 2265.108 kb on + strand, within CCNA_02115 at 2265.108 kb on + strand, within CCNA_02115 at 2265.144 kb on + strand, within CCNA_02115 at 2265.201 kb on + strand, within CCNA_02115 at 2265.201 kb on + strand, within CCNA_02115 at 2265.201 kb on + strand, within CCNA_02115 at 2265.204 kb on - strand, within CCNA_02115 at 2265.206 kb on + strand, within CCNA_02115 at 2265.209 kb on - strand, within CCNA_02115 at 2265.209 kb on - strand, within CCNA_02115 at 2265.243 kb on + strand, within CCNA_02115 at 2265.254 kb on - strand, within CCNA_02115 at 2265.401 kb on + strand, within CCNA_02115 at 2265.434 kb on - strand, within CCNA_02115 at 2265.452 kb on + strand at 2265.472 kb on + strand at 2265.472 kb on + strand at 2265.480 kb on - strand at 2265.480 kb on - strand at 2265.503 kb on - strand at 2265.556 kb on + strand at 2265.581 kb on + strand at 2265.642 kb on + strand at 2265.716 kb on - strand at 2265.734 kb on + strand at 2265.753 kb on + strand at 2265.761 kb on - strand at 2265.789 kb on - strand at 2265.846 kb on - strand, within CCNA_02116 at 2265.867 kb on + strand, within CCNA_02116 at 2265.867 kb on + strand, within CCNA_02116 at 2265.875 kb on - strand, within CCNA_02116 at 2265.875 kb on - strand, within CCNA_02116 at 2265.920 kb on - strand, within CCNA_02116 at 2265.972 kb on - strand, within CCNA_02116 at 2265.977 kb on - strand, within CCNA_02116 at 2266.070 kb on + strand, within CCNA_02116 at 2266.078 kb on - strand, within CCNA_02116 at 2266.078 kb on - strand, within CCNA_02116 at 2266.079 kb on - strand, within CCNA_02116 at 2266.084 kb on - strand, within CCNA_02116 at 2266.143 kb on + strand, within CCNA_02116 at 2266.158 kb on + strand, within CCNA_02116 at 2266.161 kb on - strand, within CCNA_02116 at 2266.166 kb on - strand, within CCNA_02116 at 2266.212 kb on + strand, within CCNA_02116 at 2266.212 kb on + strand, within CCNA_02116 at 2266.220 kb on - strand, within CCNA_02116 at 2266.267 kb on - strand, within CCNA_02116 at 2266.363 kb on + strand, within CCNA_02116 at 2266.380 kb on + strand, within CCNA_02116 at 2266.380 kb on + strand, within CCNA_02116 at 2266.393 kb on - strand, within CCNA_02116 at 2266.414 kb on - strand, within CCNA_02116 at 2266.426 kb on - strand, within CCNA_02116 at 2266.441 kb on + strand, within CCNA_02116 at 2266.609 kb on + strand at 2266.672 kb on + strand at 2266.703 kb on + strand, within CCNA_02117 at 2266.722 kb on + strand, within CCNA_02117 at 2266.730 kb on - strand, within CCNA_02117 at 2266.746 kb on - strand, within CCNA_02117 at 2266.754 kb on - strand, within CCNA_02117 at 2266.810 kb on - strand, within CCNA_02117 at 2266.838 kb on - strand, within CCNA_02117 at 2266.839 kb on - strand, within CCNA_02117 at 2266.847 kb on + strand, within CCNA_02117 at 2266.862 kb on - strand, within CCNA_02117 at 2266.911 kb on + strand, within CCNA_02117 at 2266.926 kb on - strand, within CCNA_02117 at 2267.019 kb on + strand, within CCNA_02117 at 2267.098 kb on + strand, within CCNA_02117 at 2267.103 kb on + strand, within CCNA_02117 at 2267.108 kb on + strand, within CCNA_02117 at 2267.108 kb on + strand, within CCNA_02117 at 2267.109 kb on + strand, within CCNA_02117 at 2267.109 kb on + strand, within CCNA_02117 at 2267.111 kb on - strand, within CCNA_02117 at 2267.115 kb on + strand, within CCNA_02117 at 2267.115 kb on + strand, within CCNA_02117 at 2267.130 kb on + strand, within CCNA_02117 at 2267.130 kb on + strand, within CCNA_02117 at 2267.138 kb on - strand, within CCNA_02117 at 2267.138 kb on - strand, within CCNA_02117 at 2267.149 kb on + strand, within CCNA_02117 at 2267.149 kb on + strand, within CCNA_02117 at 2267.162 kb on + strand, within CCNA_02117 at 2267.165 kb on - strand, within CCNA_02117 at 2267.170 kb on - strand, within CCNA_02117 at 2267.231 kb on - strand at 2267.231 kb on - strand at 2267.238 kb on + strand at 2267.324 kb on - strand at 2267.405 kb on + strand at 2267.439 kb on - strand at 2267.490 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 2,264,728 + CCNA_02115 0.47 -1.5 2,264,777 + CCNA_02115 0.50 +2.9 2,264,777 + CCNA_02115 0.50 +0.9 2,264,804 - CCNA_02115 0.51 -1.5 2,264,804 - CCNA_02115 0.51 -0.0 2,264,821 + CCNA_02115 0.52 -0.4 2,264,842 + CCNA_02115 0.53 -0.0 2,264,848 - CCNA_02115 0.54 -1.7 2,264,865 - CCNA_02115 0.55 -0.5 2,264,872 + CCNA_02115 0.55 -0.9 2,264,885 + CCNA_02115 0.56 -0.3 2,264,923 + CCNA_02115 0.58 +0.2 2,264,935 + CCNA_02115 0.59 +1.6 2,264,974 + CCNA_02115 0.62 +1.6 2,264,982 - CCNA_02115 0.62 -2.2 2,264,982 - CCNA_02115 0.62 +0.6 2,264,993 + CCNA_02115 0.63 +0.3 2,264,993 + CCNA_02115 0.63 +2.5 2,265,001 - CCNA_02115 0.63 -1.5 2,265,021 - CCNA_02115 0.64 -1.4 2,265,039 - CCNA_02115 0.66 -0.9 2,265,071 + CCNA_02115 0.68 -2.7 2,265,081 - CCNA_02115 0.68 -0.7 2,265,108 + CCNA_02115 0.70 -0.5 2,265,108 + CCNA_02115 0.70 -1.2 2,265,108 + CCNA_02115 0.70 -1.4 2,265,144 + CCNA_02115 0.72 -0.1 2,265,201 + CCNA_02115 0.75 -1.8 2,265,201 + CCNA_02115 0.75 +0.6 2,265,201 + CCNA_02115 0.75 -1.3 2,265,204 - CCNA_02115 0.76 +0.5 2,265,206 + CCNA_02115 0.76 -0.9 2,265,209 - CCNA_02115 0.76 -2.5 2,265,209 - CCNA_02115 0.76 -2.4 2,265,243 + CCNA_02115 0.78 -1.8 2,265,254 - CCNA_02115 0.79 -1.3 2,265,401 + CCNA_02115 0.88 -0.1 2,265,434 - CCNA_02115 0.90 +0.5 2,265,452 + -0.0 2,265,472 + -1.9 2,265,472 + +0.9 2,265,480 - +0.4 2,265,480 - +0.5 2,265,503 - -1.0 2,265,556 + +0.0 2,265,581 + -0.9 2,265,642 + -1.1 2,265,716 - +0.3 2,265,734 + +0.5 2,265,753 + -2.6 2,265,761 - -1.8 2,265,789 - +0.2 2,265,846 - CCNA_02116 0.15 +1.3 2,265,867 + CCNA_02116 0.17 -2.3 2,265,867 + CCNA_02116 0.17 -2.6 2,265,875 - CCNA_02116 0.18 -0.6 2,265,875 - CCNA_02116 0.18 -0.0 2,265,920 - CCNA_02116 0.24 -1.7 2,265,972 - CCNA_02116 0.31 +0.0 2,265,977 - CCNA_02116 0.31 -0.4 2,266,070 + CCNA_02116 0.43 +2.2 2,266,078 - CCNA_02116 0.44 -0.9 2,266,078 - CCNA_02116 0.44 -1.4 2,266,079 - CCNA_02116 0.44 -1.3 2,266,084 - CCNA_02116 0.45 +0.2 2,266,143 + CCNA_02116 0.52 -1.6 2,266,158 + CCNA_02116 0.54 -2.1 2,266,161 - CCNA_02116 0.54 -1.5 2,266,166 - CCNA_02116 0.55 -0.2 2,266,212 + CCNA_02116 0.61 -1.1 2,266,212 + CCNA_02116 0.61 +0.1 2,266,220 - CCNA_02116 0.62 +0.4 2,266,267 - CCNA_02116 0.68 -1.0 2,266,363 + CCNA_02116 0.80 +0.6 2,266,380 + CCNA_02116 0.82 -2.6 2,266,380 + CCNA_02116 0.82 -0.8 2,266,393 - CCNA_02116 0.83 -1.3 2,266,414 - CCNA_02116 0.86 -0.8 2,266,426 - CCNA_02116 0.87 -1.1 2,266,441 + CCNA_02116 0.89 -1.3 2,266,609 + +0.6 2,266,672 + +0.2 2,266,703 + CCNA_02117 0.11 +0.6 2,266,722 + CCNA_02117 0.14 +0.9 2,266,730 - CCNA_02117 0.15 -2.9 2,266,746 - CCNA_02117 0.18 +0.6 2,266,754 - CCNA_02117 0.19 -0.3 2,266,810 - CCNA_02117 0.28 -0.9 2,266,838 - CCNA_02117 0.33 -0.1 2,266,839 - CCNA_02117 0.33 -0.4 2,266,847 + CCNA_02117 0.35 -0.2 2,266,862 - CCNA_02117 0.37 -1.4 2,266,911 + CCNA_02117 0.45 +0.1 2,266,926 - CCNA_02117 0.48 +1.0 2,267,019 + CCNA_02117 0.63 -0.5 2,267,098 + CCNA_02117 0.77 -1.3 2,267,103 + CCNA_02117 0.78 -0.5 2,267,108 + CCNA_02117 0.78 -0.1 2,267,108 + CCNA_02117 0.78 -2.8 2,267,109 + CCNA_02117 0.79 +1.5 2,267,109 + CCNA_02117 0.79 -0.2 2,267,111 - CCNA_02117 0.79 -1.7 2,267,115 + CCNA_02117 0.80 +0.3 2,267,115 + CCNA_02117 0.80 +0.1 2,267,130 + CCNA_02117 0.82 -0.2 2,267,130 + CCNA_02117 0.82 -1.4 2,267,138 - CCNA_02117 0.83 -2.3 2,267,138 - CCNA_02117 0.83 -1.1 2,267,149 + CCNA_02117 0.85 -1.2 2,267,149 + CCNA_02117 0.85 -2.9 2,267,162 + CCNA_02117 0.87 +0.4 2,267,165 - CCNA_02117 0.88 +0.4 2,267,170 - CCNA_02117 0.89 -0.1 2,267,231 - -0.2 2,267,231 - -0.2 2,267,238 + -2.2 2,267,324 - +1.5 2,267,405 + -0.3 2,267,439 - +0.7 2,267,490 + -0.1
Or see this region's nucleotide sequence