Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02068 and CCNA_02069 overlap by 1 nucleotides CCNA_02069 and CCNA_02070 are separated by 0 nucleotides CCNA_02070 and CCNA_02071 are separated by 157 nucleotides CCNA_02071 and CCNA_02072 are separated by 120 nucleotides
CCNA_02068: CCNA_02068 - Mth938-like membrane protein, at 2,219,512 to 2,219,865
_02068
CCNA_02069: CCNA_02069 - protein translocase subunit secF, at 2,219,865 to 2,220,830
_02069
CCNA_02070: CCNA_02070 - protein translocase subunit secD, at 2,220,831 to 2,222,429
_02070
CCNA_02071: CCNA_02071 - protein translocase subunit YajC, at 2,222,587 to 2,222,898
_02071
CCNA_02072: CCNA_02072 - ATP-dependent protease subunit, at 2,223,019 to 2,223,858
_02072
Position (kb)
2220
2221
2222
2223 Strain fitness (log2 ratio)
-2
-1
0
1 at 2220.821 kb on - strand at 2223.005 kb on - strand at 2223.018 kb on - strand at 2223.069 kb on - strand at 2223.102 kb on + strand at 2223.184 kb on - strand, within CCNA_02072 at 2223.270 kb on + strand, within CCNA_02072 at 2223.270 kb on + strand, within CCNA_02072 at 2223.270 kb on + strand, within CCNA_02072 at 2223.270 kb on + strand, within CCNA_02072 at 2223.270 kb on + strand, within CCNA_02072 at 2223.278 kb on - strand, within CCNA_02072 at 2223.278 kb on - strand, within CCNA_02072 at 2223.278 kb on - strand, within CCNA_02072 at 2223.278 kb on - strand, within CCNA_02072 at 2223.278 kb on - strand, within CCNA_02072 at 2223.341 kb on + strand, within CCNA_02072 at 2223.341 kb on + strand, within CCNA_02072 at 2223.344 kb on - strand, within CCNA_02072 at 2223.349 kb on - strand, within CCNA_02072 at 2223.368 kb on + strand, within CCNA_02072
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 2,220,821 - +0.3 2,223,005 - -0.2 2,223,018 - -1.5 2,223,069 - -0.9 2,223,102 + -0.1 2,223,184 - CCNA_02072 0.20 -0.4 2,223,270 + CCNA_02072 0.30 -0.7 2,223,270 + CCNA_02072 0.30 +0.0 2,223,270 + CCNA_02072 0.30 +0.9 2,223,270 + CCNA_02072 0.30 -2.0 2,223,270 + CCNA_02072 0.30 -1.2 2,223,278 - CCNA_02072 0.31 -0.2 2,223,278 - CCNA_02072 0.31 +0.3 2,223,278 - CCNA_02072 0.31 -1.0 2,223,278 - CCNA_02072 0.31 -0.7 2,223,278 - CCNA_02072 0.31 -2.3 2,223,341 + CCNA_02072 0.38 +0.4 2,223,341 + CCNA_02072 0.38 -0.3 2,223,344 - CCNA_02072 0.39 +0.8 2,223,349 - CCNA_02072 0.39 -1.0 2,223,368 + CCNA_02072 0.42 +0.8
Or see this region's nucleotide sequence