Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01809 and CCNA_01810 are separated by 3 nucleotides CCNA_01810 and CCNA_01811 overlap by 4 nucleotides CCNA_01811 and CCNA_01812 are separated by 38 nucleotides CCNA_01812 and CCNA_01813 are separated by 99 nucleotides
CCNA_01809: CCNA_01809 - lipoic acid synthetase, at 1,934,502 to 1,935,479
_01809
CCNA_01810: CCNA_01810 - coenzyme Q-binding protein, at 1,935,483 to 1,935,929
_01810
CCNA_01811: CCNA_01811 - competence/damage-inducible protein CinA, at 1,935,926 to 1,936,417
_01811
CCNA_01812: CCNA_01812 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 1,936,456 to 1,937,604
_01812
CCNA_01813: CCNA_01813 - tRNA-dihydrouridine synthase, at 1,937,704 to 1,938,711
_01813
Position (kb)
1936
1937
1938 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1935.473 kb on + strand at 1935.480 kb on + strand at 1935.488 kb on - strand at 1936.070 kb on - strand, within CCNA_01811 at 1936.123 kb on + strand, within CCNA_01811 at 1936.131 kb on + strand, within CCNA_01811 at 1936.138 kb on - strand, within CCNA_01811 at 1936.197 kb on - strand, within CCNA_01811 at 1936.228 kb on - strand, within CCNA_01811 at 1936.339 kb on - strand, within CCNA_01811 at 1936.445 kb on + strand at 1936.936 kb on - strand, within CCNA_01812 at 1937.599 kb on + strand at 1937.652 kb on + strand at 1937.737 kb on + strand at 1937.748 kb on + strand at 1937.901 kb on + strand, within CCNA_01813 at 1937.902 kb on + strand, within CCNA_01813 at 1937.929 kb on - strand, within CCNA_01813 at 1938.055 kb on + strand, within CCNA_01813 at 1938.055 kb on + strand, within CCNA_01813 at 1938.055 kb on + strand, within CCNA_01813 at 1938.055 kb on + strand, within CCNA_01813 at 1938.055 kb on + strand, within CCNA_01813 at 1938.063 kb on - strand, within CCNA_01813 at 1938.068 kb on + strand, within CCNA_01813 at 1938.068 kb on + strand, within CCNA_01813 at 1938.068 kb on + strand, within CCNA_01813 at 1938.108 kb on - strand, within CCNA_01813 at 1938.110 kb on + strand, within CCNA_01813 at 1938.134 kb on + strand, within CCNA_01813 at 1938.220 kb on + strand, within CCNA_01813 at 1938.220 kb on + strand, within CCNA_01813 at 1938.228 kb on - strand, within CCNA_01813 at 1938.254 kb on - strand, within CCNA_01813 at 1938.299 kb on - strand, within CCNA_01813 at 1938.363 kb on + strand, within CCNA_01813 at 1938.371 kb on - strand, within CCNA_01813 at 1938.429 kb on - strand, within CCNA_01813 at 1938.436 kb on + strand, within CCNA_01813 at 1938.444 kb on - strand, within CCNA_01813 at 1938.444 kb on - strand, within CCNA_01813 at 1938.478 kb on + strand, within CCNA_01813 at 1938.519 kb on + strand, within CCNA_01813 at 1938.546 kb on - strand, within CCNA_01813 at 1938.573 kb on + strand, within CCNA_01813 at 1938.573 kb on + strand, within CCNA_01813
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,935,473 + +0.9 1,935,480 + +0.2 1,935,488 - +0.4 1,936,070 - CCNA_01811 0.29 +0.6 1,936,123 + CCNA_01811 0.40 -0.3 1,936,131 + CCNA_01811 0.42 +0.2 1,936,138 - CCNA_01811 0.43 -0.6 1,936,197 - CCNA_01811 0.55 -0.1 1,936,228 - CCNA_01811 0.61 +0.4 1,936,339 - CCNA_01811 0.84 +0.7 1,936,445 + +0.6 1,936,936 - CCNA_01812 0.42 +0.2 1,937,599 + -1.9 1,937,652 + -0.0 1,937,737 + -0.0 1,937,748 + +0.2 1,937,901 + CCNA_01813 0.20 +0.5 1,937,902 + CCNA_01813 0.20 +0.0 1,937,929 - CCNA_01813 0.22 -3.7 1,938,055 + CCNA_01813 0.35 -0.2 1,938,055 + CCNA_01813 0.35 +0.4 1,938,055 + CCNA_01813 0.35 +0.4 1,938,055 + CCNA_01813 0.35 +0.3 1,938,055 + CCNA_01813 0.35 +0.4 1,938,063 - CCNA_01813 0.36 -1.5 1,938,068 + CCNA_01813 0.36 +0.4 1,938,068 + CCNA_01813 0.36 +0.4 1,938,068 + CCNA_01813 0.36 +0.9 1,938,108 - CCNA_01813 0.40 +2.8 1,938,110 + CCNA_01813 0.40 +0.5 1,938,134 + CCNA_01813 0.43 +0.2 1,938,220 + CCNA_01813 0.51 +1.2 1,938,220 + CCNA_01813 0.51 +0.2 1,938,228 - CCNA_01813 0.52 +0.1 1,938,254 - CCNA_01813 0.55 -1.9 1,938,299 - CCNA_01813 0.59 -0.2 1,938,363 + CCNA_01813 0.65 +0.4 1,938,371 - CCNA_01813 0.66 -2.1 1,938,429 - CCNA_01813 0.72 -0.7 1,938,436 + CCNA_01813 0.73 +1.7 1,938,444 - CCNA_01813 0.73 -2.1 1,938,444 - CCNA_01813 0.73 -1.8 1,938,478 + CCNA_01813 0.77 +0.8 1,938,519 + CCNA_01813 0.81 +1.3 1,938,546 - CCNA_01813 0.84 +1.2 1,938,573 + CCNA_01813 0.86 +0.5 1,938,573 + CCNA_01813 0.86 -0.2
Or see this region's nucleotide sequence