Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01504 and CCNA_01505 are separated by 42 nucleotides CCNA_01505 and CCNA_01506 are separated by 65 nucleotides CCNA_01506 and CCNA_01507 overlap by 4 nucleotides
CCNA_01504: CCNA_01504 - ATP-dependent DNA helicase recG, at 1,614,298 to 1,616,394
_01504
CCNA_01505: CCNA_01505 - hypothetical protein, at 1,616,437 to 1,616,739
_01505
CCNA_01506: CCNA_01506 - cystine transport ATP-binding protein, at 1,616,805 to 1,617,545
_01506
CCNA_01507: CCNA_01507 - cystine transport system permease protein, at 1,617,542 to 1,618,201
_01507
Position (kb)
1616
1617 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1615.564 kb on + strand, within CCNA_01504 at 1615.582 kb on - strand, within CCNA_01504 at 1615.603 kb on + strand, within CCNA_01504 at 1615.611 kb on - strand, within CCNA_01504 at 1615.676 kb on + strand, within CCNA_01504 at 1615.684 kb on - strand, within CCNA_01504 at 1615.684 kb on - strand, within CCNA_01504 at 1615.689 kb on - strand, within CCNA_01504 at 1615.731 kb on - strand, within CCNA_01504 at 1615.857 kb on + strand, within CCNA_01504 at 1615.857 kb on + strand, within CCNA_01504 at 1615.857 kb on + strand, within CCNA_01504 at 1615.857 kb on + strand, within CCNA_01504 at 1615.857 kb on + strand, within CCNA_01504 at 1615.865 kb on - strand, within CCNA_01504 at 1615.865 kb on - strand, within CCNA_01504 at 1615.865 kb on - strand, within CCNA_01504 at 1615.922 kb on - strand, within CCNA_01504 at 1615.937 kb on + strand, within CCNA_01504 at 1615.937 kb on - strand, within CCNA_01504 at 1615.961 kb on + strand, within CCNA_01504 at 1616.011 kb on + strand, within CCNA_01504 at 1616.012 kb on - strand, within CCNA_01504 at 1616.060 kb on + strand, within CCNA_01504 at 1616.068 kb on - strand, within CCNA_01504 at 1616.068 kb on - strand, within CCNA_01504 at 1616.068 kb on - strand, within CCNA_01504 at 1616.114 kb on - strand, within CCNA_01504 at 1616.155 kb on - strand, within CCNA_01504 at 1616.203 kb on + strand at 1616.229 kb on + strand at 1616.242 kb on - strand at 1616.259 kb on + strand at 1616.360 kb on + strand at 1616.360 kb on + strand at 1616.360 kb on + strand at 1616.403 kb on + strand at 1616.403 kb on + strand at 1616.533 kb on - strand, within CCNA_01505 at 1616.559 kb on + strand, within CCNA_01505 at 1616.559 kb on + strand, within CCNA_01505 at 1616.559 kb on + strand, within CCNA_01505 at 1616.567 kb on - strand, within CCNA_01505 at 1616.596 kb on + strand, within CCNA_01505 at 1616.604 kb on - strand, within CCNA_01505 at 1616.615 kb on + strand, within CCNA_01505 at 1616.685 kb on + strand, within CCNA_01505 at 1616.685 kb on + strand, within CCNA_01505 at 1616.692 kb on + strand, within CCNA_01505 at 1616.720 kb on + strand at 1616.726 kb on + strand at 1616.733 kb on + strand at 1616.877 kb on + strand at 1616.932 kb on + strand, within CCNA_01506 at 1617.009 kb on + strand, within CCNA_01506 at 1617.037 kb on - strand, within CCNA_01506 at 1617.044 kb on - strand, within CCNA_01506 at 1617.116 kb on + strand, within CCNA_01506 at 1617.126 kb on + strand, within CCNA_01506 at 1617.126 kb on + strand, within CCNA_01506 at 1617.145 kb on + strand, within CCNA_01506 at 1617.183 kb on + strand, within CCNA_01506 at 1617.192 kb on - strand, within CCNA_01506 at 1617.203 kb on + strand, within CCNA_01506 at 1617.203 kb on + strand, within CCNA_01506 at 1617.260 kb on - strand, within CCNA_01506 at 1617.312 kb on + strand, within CCNA_01506 at 1617.319 kb on + strand, within CCNA_01506 at 1617.319 kb on + strand, within CCNA_01506 at 1617.320 kb on - strand, within CCNA_01506 at 1617.327 kb on - strand, within CCNA_01506 at 1617.327 kb on - strand, within CCNA_01506 at 1617.332 kb on - strand, within CCNA_01506 at 1617.430 kb on - strand, within CCNA_01506 at 1617.430 kb on - strand, within CCNA_01506 at 1617.484 kb on - strand at 1617.586 kb on - strand at 1617.715 kb on + strand, within CCNA_01507 at 1617.726 kb on + strand, within CCNA_01507 at 1617.733 kb on - strand, within CCNA_01507 at 1617.733 kb on - strand, within CCNA_01507 at 1617.734 kb on - strand, within CCNA_01507 at 1617.734 kb on - strand, within CCNA_01507 at 1617.734 kb on - strand, within CCNA_01507
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,615,564 + CCNA_01504 0.60 -2.1 1,615,582 - CCNA_01504 0.61 -2.1 1,615,603 + CCNA_01504 0.62 -0.2 1,615,611 - CCNA_01504 0.63 +0.1 1,615,676 + CCNA_01504 0.66 -1.7 1,615,684 - CCNA_01504 0.66 +0.8 1,615,684 - CCNA_01504 0.66 +2.0 1,615,689 - CCNA_01504 0.66 +0.1 1,615,731 - CCNA_01504 0.68 +0.4 1,615,857 + CCNA_01504 0.74 -0.3 1,615,857 + CCNA_01504 0.74 -1.6 1,615,857 + CCNA_01504 0.74 -1.0 1,615,857 + CCNA_01504 0.74 -0.0 1,615,857 + CCNA_01504 0.74 -1.0 1,615,865 - CCNA_01504 0.75 -1.0 1,615,865 - CCNA_01504 0.75 +2.0 1,615,865 - CCNA_01504 0.75 +0.2 1,615,922 - CCNA_01504 0.77 -0.4 1,615,937 + CCNA_01504 0.78 -0.9 1,615,937 - CCNA_01504 0.78 -1.2 1,615,961 + CCNA_01504 0.79 +0.8 1,616,011 + CCNA_01504 0.82 -1.1 1,616,012 - CCNA_01504 0.82 -0.9 1,616,060 + CCNA_01504 0.84 -0.0 1,616,068 - CCNA_01504 0.84 -0.9 1,616,068 - CCNA_01504 0.84 +0.3 1,616,068 - CCNA_01504 0.84 +0.5 1,616,114 - CCNA_01504 0.87 -0.7 1,616,155 - CCNA_01504 0.89 +0.5 1,616,203 + -1.7 1,616,229 + -0.4 1,616,242 - +0.5 1,616,259 + -0.2 1,616,360 + +0.0 1,616,360 + +1.5 1,616,360 + -0.7 1,616,403 + -0.6 1,616,403 + -0.5 1,616,533 - CCNA_01505 0.32 +0.6 1,616,559 + CCNA_01505 0.40 +2.0 1,616,559 + CCNA_01505 0.40 -0.1 1,616,559 + CCNA_01505 0.40 -0.4 1,616,567 - CCNA_01505 0.43 +2.1 1,616,596 + CCNA_01505 0.52 -0.6 1,616,604 - CCNA_01505 0.55 +0.4 1,616,615 + CCNA_01505 0.59 -0.3 1,616,685 + CCNA_01505 0.82 -1.9 1,616,685 + CCNA_01505 0.82 +0.8 1,616,692 + CCNA_01505 0.84 -1.3 1,616,720 + -0.6 1,616,726 + +0.0 1,616,733 + -1.4 1,616,877 + -0.6 1,616,932 + CCNA_01506 0.17 -1.0 1,617,009 + CCNA_01506 0.28 -0.0 1,617,037 - CCNA_01506 0.31 -0.5 1,617,044 - CCNA_01506 0.32 +0.5 1,617,116 + CCNA_01506 0.42 +0.7 1,617,126 + CCNA_01506 0.43 +0.4 1,617,126 + CCNA_01506 0.43 -0.9 1,617,145 + CCNA_01506 0.46 +0.6 1,617,183 + CCNA_01506 0.51 +0.2 1,617,192 - CCNA_01506 0.52 -0.3 1,617,203 + CCNA_01506 0.54 +1.0 1,617,203 + CCNA_01506 0.54 +0.8 1,617,260 - CCNA_01506 0.61 -0.1 1,617,312 + CCNA_01506 0.68 +0.7 1,617,319 + CCNA_01506 0.69 +1.5 1,617,319 + CCNA_01506 0.69 -0.8 1,617,320 - CCNA_01506 0.70 +0.1 1,617,327 - CCNA_01506 0.70 +0.1 1,617,327 - CCNA_01506 0.70 -0.0 1,617,332 - CCNA_01506 0.71 +1.0 1,617,430 - CCNA_01506 0.84 +0.4 1,617,430 - CCNA_01506 0.84 +1.1 1,617,484 - +0.1 1,617,586 - -1.7 1,617,715 + CCNA_01507 0.26 +2.2 1,617,726 + CCNA_01507 0.28 -0.9 1,617,733 - CCNA_01507 0.29 +0.2 1,617,733 - CCNA_01507 0.29 +0.9 1,617,734 - CCNA_01507 0.29 +0.6 1,617,734 - CCNA_01507 0.29 -0.5 1,617,734 - CCNA_01507 0.29 +0.6
Or see this region's nucleotide sequence