Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00701 and CCNA_03888 are separated by 144 nucleotides CCNA_03888 and CCNA_00703 are separated by 102 nucleotides CCNA_00703 and CCNA_00704 are separated by 42 nucleotides CCNA_00704 and CCNA_00705 are separated by 151 nucleotides CCNA_00705 and CCNA_00706 are separated by 274 nucleotides
CCNA_00701: CCNA_00701 - cold shock protein CspB, at 761,611 to 761,820
_00701
CCNA_03888: CCNA_03888 - hypothetical protein, at 761,965 to 762,324
_03888
CCNA_00703: CCNA_00703 - hypothetical protein, at 762,427 to 762,633
_00703
CCNA_00704: CCNA_00704 - hypothetical protein, at 762,676 to 762,873
_00704
CCNA_00705: CCNA_00705 - hypothetical protein, at 763,025 to 763,315
_00705
CCNA_00706: CCNA_00706 - hypothetical protein, at 763,590 to 764,003
_00706
Position (kb) 762
763 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 761.536 kb on + strand at 761.572 kb on + strand at 761.572 kb on + strand at 761.580 kb on - strand at 761.580 kb on - strand at 761.624 kb on + strand at 761.652 kb on - strand, within CCNA_00701 at 761.662 kb on + strand, within CCNA_00701 at 761.717 kb on + strand, within CCNA_00701 at 761.718 kb on - strand, within CCNA_00701 at 761.722 kb on + strand, within CCNA_00701 at 761.722 kb on + strand, within CCNA_00701 at 761.751 kb on - strand, within CCNA_00701 at 761.805 kb on + strand at 761.839 kb on - strand at 761.844 kb on - strand at 761.934 kb on - strand at 761.958 kb on - strand at 761.967 kb on - strand at 761.967 kb on - strand at 761.968 kb on + strand at 762.035 kb on + strand, within CCNA_03888 at 762.084 kb on + strand, within CCNA_03888 at 762.084 kb on + strand, within CCNA_03888 at 762.092 kb on - strand, within CCNA_03888 at 762.092 kb on - strand, within CCNA_03888 at 762.092 kb on - strand, within CCNA_03888 at 762.092 kb on - strand, within CCNA_03888 at 762.092 kb on - strand, within CCNA_03888 at 762.092 kb on - strand, within CCNA_03888 at 762.116 kb on - strand, within CCNA_03888 at 762.157 kb on - strand, within CCNA_03888 at 762.162 kb on - strand, within CCNA_03888 at 762.162 kb on - strand, within CCNA_03888 at 762.171 kb on + strand, within CCNA_03888 at 762.256 kb on + strand, within CCNA_03888 at 762.279 kb on + strand, within CCNA_03888 at 762.281 kb on - strand, within CCNA_03888 at 762.341 kb on + strand at 762.348 kb on + strand at 762.349 kb on - strand at 762.349 kb on - strand at 762.357 kb on + strand at 762.357 kb on + strand at 762.422 kb on - strand at 762.424 kb on + strand at 762.427 kb on - strand at 762.432 kb on - strand at 762.454 kb on + strand, within CCNA_00703 at 762.467 kb on + strand, within CCNA_00703 at 762.475 kb on - strand, within CCNA_00703 at 762.488 kb on - strand, within CCNA_00703 at 762.538 kb on + strand, within CCNA_00703 at 762.543 kb on + strand, within CCNA_00703 at 762.560 kb on + strand, within CCNA_00703 at 762.560 kb on + strand, within CCNA_00703 at 762.570 kb on + strand, within CCNA_00703 at 762.572 kb on + strand, within CCNA_00703 at 762.577 kb on + strand, within CCNA_00703 at 762.577 kb on + strand, within CCNA_00703 at 762.577 kb on + strand, within CCNA_00703 at 762.577 kb on + strand, within CCNA_00703 at 762.582 kb on + strand, within CCNA_00703 at 762.583 kb on + strand, within CCNA_00703 at 762.585 kb on - strand, within CCNA_00703 at 762.585 kb on - strand, within CCNA_00703 at 762.590 kb on - strand, within CCNA_00703 at 762.590 kb on - strand, within CCNA_00703 at 762.650 kb on + strand at 762.696 kb on + strand, within CCNA_00704 at 762.708 kb on + strand, within CCNA_00704 at 762.718 kb on - strand, within CCNA_00704 at 762.745 kb on + strand, within CCNA_00704 at 762.785 kb on - strand, within CCNA_00704 at 762.800 kb on - strand, within CCNA_00704 at 762.819 kb on - strand, within CCNA_00704 at 762.821 kb on + strand, within CCNA_00704 at 762.836 kb on - strand, within CCNA_00704 at 762.856 kb on + strand at 762.894 kb on + strand at 762.894 kb on + strand at 762.894 kb on + strand at 762.906 kb on + strand at 762.914 kb on - strand at 762.914 kb on - strand at 762.916 kb on + strand at 762.916 kb on + strand at 762.916 kb on + strand at 762.924 kb on - strand at 762.951 kb on + strand at 762.951 kb on + strand at 762.959 kb on - strand at 763.018 kb on - strand at 763.055 kb on + strand, within CCNA_00705 at 763.063 kb on - strand, within CCNA_00705 at 763.064 kb on + strand, within CCNA_00705 at 763.087 kb on - strand, within CCNA_00705 at 763.170 kb on - strand, within CCNA_00705 at 763.190 kb on + strand, within CCNA_00705 at 763.245 kb on + strand, within CCNA_00705 at 763.281 kb on - strand, within CCNA_00705 at 763.281 kb on - strand, within CCNA_00705 at 763.345 kb on - strand at 763.350 kb on - strand at 763.373 kb on - strand at 763.420 kb on - strand at 763.477 kb on + strand at 763.518 kb on - strand at 763.547 kb on - strand at 763.574 kb on + strand at 763.608 kb on + strand at 763.618 kb on - strand at 763.623 kb on + strand at 763.631 kb on - strand at 763.631 kb on - strand at 763.632 kb on - strand, within CCNA_00706
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 761,536 + +0.9 761,572 + -0.3 761,572 + +1.3 761,580 - +0.7 761,580 - -1.5 761,624 + +1.5 761,652 - CCNA_00701 0.20 -1.6 761,662 + CCNA_00701 0.24 -0.1 761,717 + CCNA_00701 0.50 +0.4 761,718 - CCNA_00701 0.51 -0.7 761,722 + CCNA_00701 0.53 -0.0 761,722 + CCNA_00701 0.53 -2.5 761,751 - CCNA_00701 0.67 -2.9 761,805 + +0.2 761,839 - -0.2 761,844 - +0.7 761,934 - +0.9 761,958 - -0.3 761,967 - +0.8 761,967 - +0.2 761,968 + -0.2 762,035 + CCNA_03888 0.19 -1.0 762,084 + CCNA_03888 0.33 +1.0 762,084 + CCNA_03888 0.33 -0.2 762,092 - CCNA_03888 0.35 +0.1 762,092 - CCNA_03888 0.35 +0.8 762,092 - CCNA_03888 0.35 +0.0 762,092 - CCNA_03888 0.35 +0.2 762,092 - CCNA_03888 0.35 +0.4 762,092 - CCNA_03888 0.35 +0.5 762,116 - CCNA_03888 0.42 +0.0 762,157 - CCNA_03888 0.53 -0.1 762,162 - CCNA_03888 0.55 -1.4 762,162 - CCNA_03888 0.55 -0.2 762,171 + CCNA_03888 0.57 +0.4 762,256 + CCNA_03888 0.81 -0.5 762,279 + CCNA_03888 0.87 -0.6 762,281 - CCNA_03888 0.88 -0.3 762,341 + +0.8 762,348 + +0.7 762,349 - +0.0 762,349 - -1.0 762,357 + +0.0 762,357 + -0.3 762,422 - +0.5 762,424 + +0.8 762,427 - +0.4 762,432 - +0.3 762,454 + CCNA_00703 0.13 +0.7 762,467 + CCNA_00703 0.19 -2.3 762,475 - CCNA_00703 0.23 -0.3 762,488 - CCNA_00703 0.29 -0.4 762,538 + CCNA_00703 0.54 +0.1 762,543 + CCNA_00703 0.56 +0.3 762,560 + CCNA_00703 0.64 +0.4 762,560 + CCNA_00703 0.64 -3.8 762,570 + CCNA_00703 0.69 +0.7 762,572 + CCNA_00703 0.70 -0.9 762,577 + CCNA_00703 0.72 +0.1 762,577 + CCNA_00703 0.72 +0.4 762,577 + CCNA_00703 0.72 -0.1 762,577 + CCNA_00703 0.72 -0.6 762,582 + CCNA_00703 0.75 +1.1 762,583 + CCNA_00703 0.75 +0.4 762,585 - CCNA_00703 0.76 -0.9 762,585 - CCNA_00703 0.76 -0.6 762,590 - CCNA_00703 0.79 +0.5 762,590 - CCNA_00703 0.79 +0.7 762,650 + +1.9 762,696 + CCNA_00704 0.10 +0.0 762,708 + CCNA_00704 0.16 +0.8 762,718 - CCNA_00704 0.21 +0.7 762,745 + CCNA_00704 0.35 +0.5 762,785 - CCNA_00704 0.55 -2.3 762,800 - CCNA_00704 0.63 -1.5 762,819 - CCNA_00704 0.72 -1.5 762,821 + CCNA_00704 0.73 +0.6 762,836 - CCNA_00704 0.81 -0.6 762,856 + +0.1 762,894 + -0.8 762,894 + +0.8 762,894 + +0.5 762,906 + +0.4 762,914 - +0.3 762,914 - -0.1 762,916 + +0.6 762,916 + -0.1 762,916 + +0.1 762,924 - +0.6 762,951 + +0.8 762,951 + +0.4 762,959 - -1.0 763,018 - +0.5 763,055 + CCNA_00705 0.10 -1.4 763,063 - CCNA_00705 0.13 +0.3 763,064 + CCNA_00705 0.13 -0.1 763,087 - CCNA_00705 0.21 -0.2 763,170 - CCNA_00705 0.50 +0.4 763,190 + CCNA_00705 0.57 +0.7 763,245 + CCNA_00705 0.76 -0.1 763,281 - CCNA_00705 0.88 +0.2 763,281 - CCNA_00705 0.88 +0.7 763,345 - +0.2 763,350 - -1.9 763,373 - +0.9 763,420 - +0.6 763,477 + +0.7 763,518 - -0.3 763,547 - -1.5 763,574 + -0.3 763,608 + -0.7 763,618 - +1.2 763,623 + -0.5 763,631 - -0.7 763,631 - +1.1 763,632 - CCNA_00706 0.10 +1.0
Or see this region's nucleotide sequence