Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00525 and CCNA_00526 are separated by 114 nucleotides CCNA_00526 and CCNA_00527 are separated by 130 nucleotides CCNA_00527 and CCNA_00528 are separated by 180 nucleotides
CCNA_00525: CCNA_00525 - prolipoprotein diacylglyceryl transferase, at 538,746 to 539,654
_00525
CCNA_00526: CCNA_00526 - BMFP membrane fusogenic protein, at 539,769 to 540,026
_00526
CCNA_00527: CCNA_00527 - hypothetical protein, at 540,157 to 540,663
_00527
CCNA_00528: CCNA_00528 - pyrroline-5-carboxylate reductase, at 540,844 to 541,626
_00528
Position (kb) 539
540
541 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 539.658 kb on + strand at 539.666 kb on - strand at 539.676 kb on - strand at 539.678 kb on + strand at 539.681 kb on - strand at 539.681 kb on - strand at 539.683 kb on + strand at 539.686 kb on - strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.813 kb on - strand, within CCNA_00526 at 539.860 kb on - strand, within CCNA_00526 at 539.878 kb on - strand, within CCNA_00526 at 539.954 kb on + strand, within CCNA_00526 at 539.980 kb on - strand, within CCNA_00526 at 540.040 kb on - strand at 540.042 kb on + strand at 540.046 kb on - strand at 540.050 kb on - strand at 540.059 kb on + strand at 540.082 kb on + strand at 540.090 kb on - strand at 540.099 kb on - strand at 540.106 kb on + strand at 540.116 kb on + strand at 540.116 kb on + strand at 540.116 kb on + strand at 540.116 kb on + strand at 540.119 kb on - strand at 540.119 kb on - strand at 540.124 kb on - strand at 540.124 kb on - strand at 540.151 kb on - strand at 540.202 kb on + strand at 540.203 kb on + strand at 540.203 kb on + strand at 540.211 kb on - strand, within CCNA_00527 at 540.213 kb on + strand, within CCNA_00527 at 540.245 kb on + strand, within CCNA_00527 at 540.253 kb on - strand, within CCNA_00527 at 540.253 kb on - strand, within CCNA_00527 at 540.268 kb on + strand, within CCNA_00527 at 540.293 kb on + strand, within CCNA_00527 at 540.293 kb on + strand, within CCNA_00527 at 540.308 kb on - strand, within CCNA_00527 at 540.327 kb on + strand, within CCNA_00527 at 540.335 kb on - strand, within CCNA_00527 at 540.341 kb on + strand, within CCNA_00527 at 540.341 kb on + strand, within CCNA_00527 at 540.341 kb on + strand, within CCNA_00527 at 540.341 kb on + strand, within CCNA_00527 at 540.346 kb on + strand, within CCNA_00527 at 540.349 kb on - strand, within CCNA_00527 at 540.349 kb on - strand, within CCNA_00527 at 540.349 kb on - strand, within CCNA_00527 at 540.359 kb on + strand, within CCNA_00527 at 540.359 kb on + strand, within CCNA_00527 at 540.367 kb on - strand, within CCNA_00527 at 540.389 kb on - strand, within CCNA_00527 at 540.398 kb on + strand, within CCNA_00527 at 540.398 kb on + strand, within CCNA_00527 at 540.450 kb on + strand, within CCNA_00527 at 540.451 kb on + strand, within CCNA_00527 at 540.487 kb on + strand, within CCNA_00527 at 540.487 kb on + strand, within CCNA_00527 at 540.488 kb on + strand, within CCNA_00527 at 540.496 kb on - strand, within CCNA_00527 at 540.496 kb on - strand, within CCNA_00527 at 540.498 kb on + strand, within CCNA_00527 at 540.498 kb on + strand, within CCNA_00527 at 540.498 kb on + strand, within CCNA_00527 at 540.498 kb on + strand, within CCNA_00527 at 540.498 kb on + strand, within CCNA_00527 at 540.506 kb on - strand, within CCNA_00527 at 540.511 kb on + strand, within CCNA_00527 at 540.523 kb on + strand, within CCNA_00527 at 540.531 kb on - strand, within CCNA_00527 at 540.603 kb on + strand, within CCNA_00527 at 540.629 kb on - strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.664 kb on + strand at 540.664 kb on + strand at 540.664 kb on + strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.718 kb on + strand at 540.721 kb on - strand at 540.721 kb on - strand at 540.726 kb on - strand at 540.726 kb on - strand at 540.751 kb on + strand at 540.751 kb on + strand at 540.751 kb on + strand at 540.751 kb on + strand at 540.752 kb on + strand at 540.759 kb on - strand at 540.759 kb on - strand at 540.759 kb on - strand at 540.759 kb on - strand at 540.813 kb on + strand at 540.820 kb on + strand at 540.828 kb on - strand at 540.848 kb on + strand at 540.848 kb on + strand at 540.856 kb on - strand at 540.879 kb on - strand at 540.892 kb on + strand at 540.892 kb on + strand at 540.894 kb on - strand at 540.894 kb on - strand at 540.900 kb on - strand at 540.925 kb on - strand, within CCNA_00528 at 540.954 kb on + strand, within CCNA_00528 at 540.960 kb on - strand, within CCNA_00528 at 540.962 kb on - strand, within CCNA_00528 at 540.972 kb on - strand, within CCNA_00528 at 540.975 kb on - strand, within CCNA_00528 at 540.992 kb on - strand, within CCNA_00528 at 540.992 kb on - strand, within CCNA_00528 at 541.014 kb on + strand, within CCNA_00528 at 541.022 kb on - strand, within CCNA_00528 at 541.023 kb on - strand, within CCNA_00528
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 539,658 + -0.5 539,666 - +0.3 539,676 - +0.3 539,678 + -0.5 539,681 - -0.0 539,681 - +0.1 539,683 + -0.2 539,686 - -0.4 539,688 + -1.8 539,688 + -1.7 539,688 + +0.4 539,688 + -1.3 539,688 + -0.8 539,688 + +0.3 539,688 + -0.8 539,688 + -0.5 539,688 + -0.1 539,688 + -1.4 539,688 + +0.4 539,688 + -1.3 539,688 + -1.3 539,688 + -0.7 539,688 + -0.0 539,688 + +0.0 539,688 + +2.3 539,688 + -1.9 539,688 + -2.3 539,688 + +0.1 539,688 + -1.3 539,688 + -1.3 539,688 + -0.0 539,688 + +0.3 539,688 + -2.0 539,688 + +0.8 539,688 + -0.4 539,688 + -1.8 539,688 + -2.2 539,688 + -0.5 539,688 + -2.0 539,688 + +0.0 539,688 + -1.7 539,696 - +2.6 539,696 - +0.4 539,696 - +0.4 539,696 - +0.5 539,696 - -1.2 539,696 - +0.0 539,696 - +0.0 539,696 - -0.3 539,696 - -0.5 539,696 - -2.4 539,696 - -2.3 539,696 - +1.2 539,696 - +2.3 539,696 - -0.3 539,696 - +0.2 539,696 - -0.7 539,696 - +0.4 539,696 - +1.1 539,696 - +0.4 539,696 - -0.2 539,696 - +0.6 539,696 - -0.2 539,696 - -2.4 539,696 - +0.6 539,696 - +0.4 539,696 - -1.1 539,696 - +0.2 539,696 - +0.2 539,696 - +1.0 539,696 - -0.0 539,696 - +0.5 539,696 - -0.2 539,696 - +3.5 539,696 - +0.1 539,696 - +0.8 539,696 - -1.0 539,813 - CCNA_00526 0.17 -0.1 539,860 - CCNA_00526 0.35 -0.0 539,878 - CCNA_00526 0.42 +1.6 539,954 + CCNA_00526 0.72 -0.0 539,980 - CCNA_00526 0.82 -0.2 540,040 - -1.8 540,042 + +1.0 540,046 - -0.3 540,050 - -0.7 540,059 + +0.3 540,082 + +0.2 540,090 - +0.6 540,099 - +0.9 540,106 + +0.4 540,116 + +0.9 540,116 + +1.6 540,116 + -0.5 540,116 + +0.1 540,119 - -0.8 540,119 - -0.0 540,124 - +0.5 540,124 - +1.8 540,151 - -0.7 540,202 + +0.2 540,203 + +0.3 540,203 + +0.5 540,211 - CCNA_00527 0.11 +0.1 540,213 + CCNA_00527 0.11 +0.4 540,245 + CCNA_00527 0.17 +0.1 540,253 - CCNA_00527 0.19 -1.5 540,253 - CCNA_00527 0.19 -0.7 540,268 + CCNA_00527 0.22 +0.3 540,293 + CCNA_00527 0.27 -0.3 540,293 + CCNA_00527 0.27 +0.5 540,308 - CCNA_00527 0.30 +0.4 540,327 + CCNA_00527 0.34 +0.0 540,335 - CCNA_00527 0.35 +1.4 540,341 + CCNA_00527 0.36 -0.7 540,341 + CCNA_00527 0.36 +0.2 540,341 + CCNA_00527 0.36 +0.5 540,341 + CCNA_00527 0.36 -0.0 540,346 + CCNA_00527 0.37 +0.6 540,349 - CCNA_00527 0.38 -0.0 540,349 - CCNA_00527 0.38 -0.8 540,349 - CCNA_00527 0.38 -0.4 540,359 + CCNA_00527 0.40 -0.6 540,359 + CCNA_00527 0.40 +0.7 540,367 - CCNA_00527 0.41 +0.6 540,389 - CCNA_00527 0.46 -1.0 540,398 + CCNA_00527 0.48 -0.1 540,398 + CCNA_00527 0.48 +0.0 540,450 + CCNA_00527 0.58 -0.2 540,451 + CCNA_00527 0.58 +0.1 540,487 + CCNA_00527 0.65 -0.0 540,487 + CCNA_00527 0.65 +0.2 540,488 + CCNA_00527 0.65 +0.5 540,496 - CCNA_00527 0.67 +0.0 540,496 - CCNA_00527 0.67 -1.5 540,498 + CCNA_00527 0.67 +0.1 540,498 + CCNA_00527 0.67 +0.5 540,498 + CCNA_00527 0.67 +0.5 540,498 + CCNA_00527 0.67 +0.3 540,498 + CCNA_00527 0.67 +0.4 540,506 - CCNA_00527 0.69 -1.8 540,511 + CCNA_00527 0.70 +0.1 540,523 + CCNA_00527 0.72 +0.2 540,531 - CCNA_00527 0.74 +0.2 540,603 + CCNA_00527 0.88 +0.1 540,629 - +1.4 540,657 + +0.4 540,657 + +0.6 540,657 + +0.1 540,657 + -0.0 540,657 + +0.0 540,657 + +0.7 540,657 + -0.2 540,657 + +0.5 540,657 + +0.1 540,657 + +0.2 540,657 + +0.2 540,657 + +0.4 540,664 + -1.8 540,664 + +0.4 540,664 + +0.0 540,665 - -0.7 540,665 - +0.4 540,665 - +0.9 540,665 - -0.7 540,665 - -3.8 540,665 - -1.0 540,665 - +0.2 540,665 - +2.2 540,665 - -0.5 540,665 - +0.8 540,665 - -2.7 540,665 - -0.6 540,718 + +0.3 540,721 - +1.0 540,721 - -1.3 540,726 - -1.8 540,726 - +0.1 540,751 + -0.0 540,751 + +0.6 540,751 + +0.6 540,751 + +0.5 540,752 + -0.4 540,759 - -0.1 540,759 - +1.3 540,759 - -0.6 540,759 - +0.5 540,813 + -0.0 540,820 + -0.7 540,828 - -0.5 540,848 + +0.4 540,848 + -0.1 540,856 - -1.2 540,879 - +0.3 540,892 + -0.5 540,892 + -1.0 540,894 - -1.3 540,894 - +0.1 540,900 - +1.7 540,925 - CCNA_00528 0.10 -0.7 540,954 + CCNA_00528 0.14 +0.9 540,960 - CCNA_00528 0.15 +0.1 540,962 - CCNA_00528 0.15 +0.1 540,972 - CCNA_00528 0.16 -0.3 540,975 - CCNA_00528 0.17 +0.4 540,992 - CCNA_00528 0.19 -0.4 540,992 - CCNA_00528 0.19 -2.2 541,014 + CCNA_00528 0.22 +0.7 541,022 - CCNA_00528 0.23 -0.8 541,023 - CCNA_00528 0.23 -0.5
Or see this region's nucleotide sequence