Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00326 and CCNA_00327 are separated by 162 nucleotides CCNA_00327 and CCNA_00328 are separated by 84 nucleotides CCNA_00328 and CCNA_00329 are separated by 12 nucleotides
CCNA_00326: CCNA_00326 - vegetatible incompatibility protein HET-E-1, at 338,634 to 339,665
_00326
CCNA_00327: CCNA_00327 - FMN-binding nitroreductase family enzyme, at 339,828 to 340,490
_00327
CCNA_00328: CCNA_00328 - monofunctional biosynthetic peptidoglycan transglycosylase MtgA, at 340,575 to 341,264
_00328
CCNA_00329: CCNA_00329 - ribosomal-protein-alanine acetyltransferase, at 341,277 to 341,825
_00329
Position (kb) 339
340
341 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 338.850 kb on + strand, within CCNA_00326 at 338.850 kb on + strand, within CCNA_00326 at 338.877 kb on + strand, within CCNA_00326 at 338.884 kb on - strand, within CCNA_00326 at 338.941 kb on + strand, within CCNA_00326 at 338.949 kb on - strand, within CCNA_00326 at 338.949 kb on - strand, within CCNA_00326 at 338.976 kb on + strand, within CCNA_00326 at 339.042 kb on + strand, within CCNA_00326 at 339.050 kb on - strand, within CCNA_00326 at 339.050 kb on - strand, within CCNA_00326 at 339.062 kb on + strand, within CCNA_00326 at 339.072 kb on - strand, within CCNA_00326 at 339.099 kb on + strand, within CCNA_00326 at 339.107 kb on - strand, within CCNA_00326 at 339.107 kb on - strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.146 kb on - strand, within CCNA_00326 at 339.146 kb on - strand, within CCNA_00326 at 339.146 kb on - strand, within CCNA_00326 at 339.165 kb on - strand, within CCNA_00326 at 339.168 kb on + strand, within CCNA_00326 at 339.168 kb on + strand, within CCNA_00326 at 339.191 kb on + strand, within CCNA_00326 at 339.191 kb on + strand, within CCNA_00326 at 339.197 kb on + strand, within CCNA_00326 at 339.200 kb on - strand, within CCNA_00326 at 339.229 kb on + strand, within CCNA_00326 at 339.281 kb on + strand, within CCNA_00326 at 339.308 kb on + strand, within CCNA_00326 at 339.308 kb on + strand, within CCNA_00326 at 339.309 kb on + strand, within CCNA_00326 at 339.342 kb on - strand, within CCNA_00326 at 339.381 kb on + strand, within CCNA_00326 at 339.398 kb on + strand, within CCNA_00326 at 339.450 kb on + strand, within CCNA_00326 at 339.468 kb on + strand, within CCNA_00326 at 339.468 kb on + strand, within CCNA_00326 at 339.468 kb on + strand, within CCNA_00326 at 339.468 kb on + strand, within CCNA_00326 at 339.476 kb on - strand, within CCNA_00326 at 339.480 kb on + strand, within CCNA_00326 at 339.480 kb on + strand, within CCNA_00326 at 339.481 kb on - strand, within CCNA_00326 at 339.485 kb on + strand, within CCNA_00326 at 339.485 kb on + strand, within CCNA_00326 at 339.485 kb on + strand, within CCNA_00326 at 339.493 kb on - strand, within CCNA_00326 at 339.519 kb on - strand, within CCNA_00326 at 339.524 kb on + strand, within CCNA_00326 at 339.524 kb on + strand, within CCNA_00326 at 339.532 kb on - strand, within CCNA_00326 at 339.539 kb on - strand, within CCNA_00326 at 339.548 kb on + strand, within CCNA_00326 at 339.561 kb on - strand, within CCNA_00326 at 339.595 kb on - strand at 339.595 kb on - strand at 339.618 kb on - strand at 339.660 kb on + strand at 339.663 kb on - strand at 339.668 kb on - strand at 339.689 kb on - strand at 339.689 kb on - strand at 339.699 kb on - strand at 339.756 kb on - strand at 339.777 kb on - strand at 339.777 kb on - strand at 339.791 kb on - strand at 339.806 kb on + strand at 339.828 kb on + strand at 339.834 kb on - strand at 339.837 kb on + strand at 339.845 kb on - strand at 339.915 kb on - strand, within CCNA_00327 at 339.920 kb on - strand, within CCNA_00327 at 339.941 kb on - strand, within CCNA_00327 at 339.954 kb on + strand, within CCNA_00327 at 339.961 kb on - strand, within CCNA_00327 at 339.974 kb on + strand, within CCNA_00327 at 339.974 kb on + strand, within CCNA_00327 at 339.974 kb on + strand, within CCNA_00327 at 339.974 kb on + strand, within CCNA_00327 at 339.978 kb on + strand, within CCNA_00327 at 339.982 kb on - strand, within CCNA_00327 at 339.982 kb on - strand, within CCNA_00327 at 339.988 kb on + strand, within CCNA_00327 at 339.988 kb on + strand, within CCNA_00327 at 339.993 kb on + strand, within CCNA_00327 at 340.008 kb on + strand, within CCNA_00327 at 340.010 kb on + strand, within CCNA_00327 at 340.016 kb on - strand, within CCNA_00327 at 340.016 kb on - strand, within CCNA_00327 at 340.037 kb on + strand, within CCNA_00327 at 340.045 kb on - strand, within CCNA_00327 at 340.045 kb on - strand, within CCNA_00327 at 340.045 kb on - strand, within CCNA_00327 at 340.055 kb on - strand, within CCNA_00327 at 340.055 kb on - strand, within CCNA_00327 at 340.079 kb on - strand, within CCNA_00327 at 340.134 kb on - strand, within CCNA_00327 at 340.139 kb on - strand, within CCNA_00327 at 340.150 kb on + strand, within CCNA_00327 at 340.168 kb on + strand, within CCNA_00327 at 340.176 kb on - strand, within CCNA_00327 at 340.194 kb on - strand, within CCNA_00327 at 340.196 kb on - strand, within CCNA_00327 at 340.198 kb on + strand, within CCNA_00327 at 340.200 kb on + strand, within CCNA_00327 at 340.206 kb on - strand, within CCNA_00327 at 340.208 kb on - strand, within CCNA_00327 at 340.210 kb on + strand, within CCNA_00327 at 340.213 kb on - strand, within CCNA_00327 at 340.225 kb on - strand, within CCNA_00327 at 340.251 kb on - strand, within CCNA_00327 at 340.251 kb on - strand, within CCNA_00327 at 340.251 kb on - strand, within CCNA_00327 at 340.258 kb on - strand, within CCNA_00327 at 340.269 kb on + strand, within CCNA_00327 at 340.274 kb on - strand, within CCNA_00327 at 340.291 kb on + strand, within CCNA_00327 at 340.353 kb on - strand, within CCNA_00327 at 340.362 kb on - strand, within CCNA_00327 at 340.374 kb on + strand, within CCNA_00327 at 340.396 kb on + strand, within CCNA_00327 at 340.398 kb on + strand, within CCNA_00327 at 340.398 kb on + strand, within CCNA_00327 at 340.404 kb on - strand, within CCNA_00327 at 340.423 kb on + strand, within CCNA_00327 at 340.423 kb on + strand, within CCNA_00327 at 340.441 kb on - strand at 340.475 kb on - strand at 340.499 kb on + strand at 340.507 kb on - strand at 340.513 kb on + strand at 340.516 kb on - strand at 340.521 kb on - strand at 340.521 kb on - strand at 340.545 kb on + strand at 340.711 kb on - strand, within CCNA_00328 at 340.713 kb on + strand, within CCNA_00328 at 340.713 kb on + strand, within CCNA_00328 at 340.721 kb on - strand, within CCNA_00328 at 340.726 kb on - strand, within CCNA_00328 at 340.733 kb on - strand, within CCNA_00328 at 340.770 kb on + strand, within CCNA_00328 at 340.786 kb on - strand, within CCNA_00328 at 340.815 kb on + strand, within CCNA_00328 at 340.836 kb on - strand, within CCNA_00328 at 340.896 kb on - strand, within CCNA_00328 at 340.945 kb on - strand, within CCNA_00328 at 340.956 kb on + strand, within CCNA_00328 at 340.956 kb on + strand, within CCNA_00328 at 340.964 kb on - strand, within CCNA_00328 at 340.972 kb on - strand, within CCNA_00328 at 340.977 kb on + strand, within CCNA_00328 at 340.982 kb on + strand, within CCNA_00328 at 340.990 kb on - strand, within CCNA_00328 at 340.999 kb on - strand, within CCNA_00328 at 341.078 kb on + strand, within CCNA_00328 at 341.078 kb on + strand, within CCNA_00328 at 341.098 kb on + strand, within CCNA_00328 at 341.114 kb on + strand, within CCNA_00328 at 341.118 kb on + strand, within CCNA_00328 at 341.118 kb on - strand, within CCNA_00328 at 341.139 kb on + strand, within CCNA_00328 at 341.144 kb on + strand, within CCNA_00328 at 341.144 kb on + strand, within CCNA_00328 at 341.147 kb on - strand, within CCNA_00328 at 341.147 kb on - strand, within CCNA_00328 at 341.216 kb on + strand at 341.216 kb on + strand at 341.216 kb on + strand at 341.224 kb on - strand at 341.250 kb on - strand at 341.254 kb on - strand at 341.287 kb on - strand at 341.292 kb on + strand at 341.308 kb on + strand at 341.308 kb on - strand at 341.313 kb on - strand at 341.343 kb on + strand, within CCNA_00329 at 341.343 kb on + strand, within CCNA_00329 at 341.348 kb on + strand, within CCNA_00329 at 341.351 kb on - strand, within CCNA_00329 at 341.351 kb on - strand, within CCNA_00329 at 341.351 kb on - strand, within CCNA_00329 at 341.366 kb on - strand, within CCNA_00329 at 341.371 kb on - strand, within CCNA_00329 at 341.371 kb on - strand, within CCNA_00329 at 341.371 kb on - strand, within CCNA_00329 at 341.403 kb on + strand, within CCNA_00329 at 341.421 kb on + strand, within CCNA_00329 at 341.429 kb on - strand, within CCNA_00329 at 341.457 kb on + strand, within CCNA_00329 at 341.467 kb on + strand, within CCNA_00329 at 341.475 kb on - strand, within CCNA_00329 at 341.478 kb on + strand, within CCNA_00329
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 338,850 + CCNA_00326 0.21 -1.9 338,850 + CCNA_00326 0.21 +0.0 338,877 + CCNA_00326 0.24 -0.4 338,884 - CCNA_00326 0.24 +1.3 338,941 + CCNA_00326 0.30 +1.1 338,949 - CCNA_00326 0.31 +0.8 338,949 - CCNA_00326 0.31 -1.2 338,976 + CCNA_00326 0.33 +0.7 339,042 + CCNA_00326 0.40 +0.4 339,050 - CCNA_00326 0.40 +0.7 339,050 - CCNA_00326 0.40 +0.4 339,062 + CCNA_00326 0.41 +1.2 339,072 - CCNA_00326 0.42 +0.3 339,099 + CCNA_00326 0.45 +0.5 339,107 - CCNA_00326 0.46 -1.2 339,107 - CCNA_00326 0.46 -0.7 339,138 + CCNA_00326 0.49 +0.6 339,138 + CCNA_00326 0.49 +0.2 339,138 + CCNA_00326 0.49 -0.3 339,146 - CCNA_00326 0.50 -0.3 339,146 - CCNA_00326 0.50 -1.0 339,146 - CCNA_00326 0.50 +0.1 339,165 - CCNA_00326 0.51 +0.0 339,168 + CCNA_00326 0.52 -0.8 339,168 + CCNA_00326 0.52 +1.1 339,191 + CCNA_00326 0.54 -0.0 339,191 + CCNA_00326 0.54 +0.2 339,197 + CCNA_00326 0.55 -1.7 339,200 - CCNA_00326 0.55 +0.4 339,229 + CCNA_00326 0.58 +0.2 339,281 + CCNA_00326 0.63 +0.2 339,308 + CCNA_00326 0.65 +0.2 339,308 + CCNA_00326 0.65 +0.7 339,309 + CCNA_00326 0.65 -0.8 339,342 - CCNA_00326 0.69 -0.4 339,381 + CCNA_00326 0.72 -1.0 339,398 + CCNA_00326 0.74 -0.7 339,450 + CCNA_00326 0.79 +0.1 339,468 + CCNA_00326 0.81 -0.2 339,468 + CCNA_00326 0.81 -0.1 339,468 + CCNA_00326 0.81 +0.7 339,468 + CCNA_00326 0.81 +0.0 339,476 - CCNA_00326 0.82 -0.7 339,480 + CCNA_00326 0.82 +0.6 339,480 + CCNA_00326 0.82 +0.3 339,481 - CCNA_00326 0.82 -0.1 339,485 + CCNA_00326 0.82 -0.3 339,485 + CCNA_00326 0.82 -0.7 339,485 + CCNA_00326 0.82 +0.2 339,493 - CCNA_00326 0.83 -0.1 339,519 - CCNA_00326 0.86 +0.5 339,524 + CCNA_00326 0.86 -0.8 339,524 + CCNA_00326 0.86 +0.3 339,532 - CCNA_00326 0.87 -0.8 339,539 - CCNA_00326 0.88 -0.3 339,548 + CCNA_00326 0.89 +0.2 339,561 - CCNA_00326 0.90 -0.8 339,595 - +0.1 339,595 - -1.5 339,618 - +0.1 339,660 + +0.0 339,663 - -0.1 339,668 - -0.1 339,689 - +0.1 339,689 - +0.2 339,699 - +0.0 339,756 - +0.9 339,777 - +0.4 339,777 - +0.0 339,791 - -0.4 339,806 + +0.1 339,828 + -0.1 339,834 - -0.4 339,837 + +0.5 339,845 - +0.1 339,915 - CCNA_00327 0.13 -1.2 339,920 - CCNA_00327 0.14 +0.3 339,941 - CCNA_00327 0.17 -0.3 339,954 + CCNA_00327 0.19 -1.0 339,961 - CCNA_00327 0.20 +1.2 339,974 + CCNA_00327 0.22 -0.6 339,974 + CCNA_00327 0.22 -0.1 339,974 + CCNA_00327 0.22 -0.1 339,974 + CCNA_00327 0.22 -2.2 339,978 + CCNA_00327 0.23 -0.5 339,982 - CCNA_00327 0.23 -0.5 339,982 - CCNA_00327 0.23 +0.6 339,988 + CCNA_00327 0.24 +0.4 339,988 + CCNA_00327 0.24 -2.0 339,993 + CCNA_00327 0.25 +0.8 340,008 + CCNA_00327 0.27 +0.2 340,010 + CCNA_00327 0.27 -0.7 340,016 - CCNA_00327 0.28 +0.1 340,016 - CCNA_00327 0.28 -1.4 340,037 + CCNA_00327 0.32 +0.0 340,045 - CCNA_00327 0.33 +0.5 340,045 - CCNA_00327 0.33 +1.0 340,045 - CCNA_00327 0.33 -0.2 340,055 - CCNA_00327 0.34 -0.0 340,055 - CCNA_00327 0.34 +0.7 340,079 - CCNA_00327 0.38 -0.7 340,134 - CCNA_00327 0.46 -0.1 340,139 - CCNA_00327 0.47 -0.3 340,150 + CCNA_00327 0.49 -0.5 340,168 + CCNA_00327 0.51 -1.8 340,176 - CCNA_00327 0.52 +0.7 340,194 - CCNA_00327 0.55 +0.2 340,196 - CCNA_00327 0.56 +1.2 340,198 + CCNA_00327 0.56 +0.4 340,200 + CCNA_00327 0.56 -3.3 340,206 - CCNA_00327 0.57 +0.5 340,208 - CCNA_00327 0.57 +0.3 340,210 + CCNA_00327 0.58 -1.1 340,213 - CCNA_00327 0.58 -1.0 340,225 - CCNA_00327 0.60 -0.0 340,251 - CCNA_00327 0.64 -1.4 340,251 - CCNA_00327 0.64 -0.9 340,251 - CCNA_00327 0.64 +0.6 340,258 - CCNA_00327 0.65 -1.0 340,269 + CCNA_00327 0.67 -0.2 340,274 - CCNA_00327 0.67 +0.7 340,291 + CCNA_00327 0.70 +0.7 340,353 - CCNA_00327 0.79 +0.1 340,362 - CCNA_00327 0.81 -0.9 340,374 + CCNA_00327 0.82 -1.8 340,396 + CCNA_00327 0.86 -0.1 340,398 + CCNA_00327 0.86 -0.3 340,398 + CCNA_00327 0.86 -0.0 340,404 - CCNA_00327 0.87 -0.1 340,423 + CCNA_00327 0.90 -0.8 340,423 + CCNA_00327 0.90 -0.8 340,441 - -0.3 340,475 - +0.7 340,499 + -0.7 340,507 - -0.3 340,513 + -0.5 340,516 - +0.2 340,521 - +0.5 340,521 - -1.3 340,545 + -0.2 340,711 - CCNA_00328 0.20 -0.0 340,713 + CCNA_00328 0.20 +0.2 340,713 + CCNA_00328 0.20 -0.8 340,721 - CCNA_00328 0.21 -0.5 340,726 - CCNA_00328 0.22 +0.0 340,733 - CCNA_00328 0.23 -0.7 340,770 + CCNA_00328 0.28 -2.7 340,786 - CCNA_00328 0.31 +0.0 340,815 + CCNA_00328 0.35 +0.7 340,836 - CCNA_00328 0.38 -0.8 340,896 - CCNA_00328 0.47 +0.6 340,945 - CCNA_00328 0.54 +0.1 340,956 + CCNA_00328 0.55 -0.1 340,956 + CCNA_00328 0.55 -0.8 340,964 - CCNA_00328 0.56 -0.2 340,972 - CCNA_00328 0.58 +0.1 340,977 + CCNA_00328 0.58 -0.8 340,982 + CCNA_00328 0.59 +0.2 340,990 - CCNA_00328 0.60 -1.1 340,999 - CCNA_00328 0.61 -1.9 341,078 + CCNA_00328 0.73 +0.4 341,078 + CCNA_00328 0.73 -0.6 341,098 + CCNA_00328 0.76 +1.1 341,114 + CCNA_00328 0.78 -0.1 341,118 + CCNA_00328 0.79 -1.7 341,118 - CCNA_00328 0.79 -0.3 341,139 + CCNA_00328 0.82 +2.2 341,144 + CCNA_00328 0.82 +0.7 341,144 + CCNA_00328 0.82 -0.7 341,147 - CCNA_00328 0.83 -0.9 341,147 - CCNA_00328 0.83 -1.0 341,216 + +1.5 341,216 + -0.1 341,216 + -0.5 341,224 - -0.5 341,250 - -0.8 341,254 - +0.1 341,287 - +0.1 341,292 + -1.0 341,308 + +0.4 341,308 - +0.4 341,313 - -1.0 341,343 + CCNA_00329 0.12 -0.2 341,343 + CCNA_00329 0.12 +0.7 341,348 + CCNA_00329 0.13 -0.1 341,351 - CCNA_00329 0.13 -1.0 341,351 - CCNA_00329 0.13 -0.7 341,351 - CCNA_00329 0.13 -2.5 341,366 - CCNA_00329 0.16 +0.4 341,371 - CCNA_00329 0.17 -0.6 341,371 - CCNA_00329 0.17 -1.7 341,371 - CCNA_00329 0.17 +1.3 341,403 + CCNA_00329 0.23 +0.9 341,421 + CCNA_00329 0.26 -0.9 341,429 - CCNA_00329 0.28 +1.5 341,457 + CCNA_00329 0.33 +0.8 341,467 + CCNA_00329 0.35 +1.2 341,475 - CCNA_00329 0.36 +0.7 341,478 + CCNA_00329 0.37 +0.7
Or see this region's nucleotide sequence