Experiment: Trisodium citrate (C); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS04185 and PFLU_RS04190 are separated by 14 nucleotides PFLU_RS04190 and PFLU_RS04195 are separated by 267 nucleotides PFLU_RS04195 and PFLU_RS04200 overlap by 1 nucleotides
PFLU_RS04185: PFLU_RS04185 - 50S ribosomal protein L13, at 950,029 to 950,457
_RS04185
PFLU_RS04190: PFLU_RS04190 - 30S ribosomal protein S9, at 950,472 to 950,864
_RS04190
PFLU_RS04195: PFLU_RS04195 - ubiquinol-cytochrome c reductase iron-sulfur subunit, at 951,132 to 951,725
_RS04195
PFLU_RS04200: PFLU_RS04200 - cytochrome bc complex cytochrome b subunit, at 951,725 to 952,936
_RS04200
Position (kb) 951
952 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 950.862 kb on + strand at 950.862 kb on + strand at 950.872 kb on + strand at 950.872 kb on + strand at 950.872 kb on + strand at 950.933 kb on + strand at 950.933 kb on + strand at 950.933 kb on + strand at 950.934 kb on - strand at 950.934 kb on - strand at 950.935 kb on + strand at 950.935 kb on + strand at 950.936 kb on - strand at 950.979 kb on + strand at 950.981 kb on + strand at 950.981 kb on + strand at 950.981 kb on + strand at 950.981 kb on + strand at 950.981 kb on + strand at 950.981 kb on + strand at 950.981 kb on + strand at 950.982 kb on - strand at 950.982 kb on - strand at 950.982 kb on - strand at 950.982 kb on - strand at 950.982 kb on - strand at 950.982 kb on - strand at 950.982 kb on - strand at 950.984 kb on + strand at 950.984 kb on + strand at 950.984 kb on + strand at 950.984 kb on + strand at 950.984 kb on + strand at 950.984 kb on + strand at 950.985 kb on - strand at 950.985 kb on - strand at 950.985 kb on - strand at 950.985 kb on - strand at 950.985 kb on - strand at 950.985 kb on - strand at 950.985 kb on - strand at 951.025 kb on + strand at 951.025 kb on + strand at 951.025 kb on + strand at 951.026 kb on - strand at 951.026 kb on - strand at 951.026 kb on - strand at 951.026 kb on - strand at 951.026 kb on - strand at 951.043 kb on - strand at 951.043 kb on - strand at 951.063 kb on + strand at 951.064 kb on - strand at 951.067 kb on + strand at 951.067 kb on + strand at 951.067 kb on + strand at 951.067 kb on + strand at 951.067 kb on + strand at 951.068 kb on - strand at 951.068 kb on - strand at 951.068 kb on - strand at 951.068 kb on - strand at 951.068 kb on - strand at 951.068 kb on - strand at 951.068 kb on - strand at 951.068 kb on - strand at 951.082 kb on + strand at 951.082 kb on + strand at 951.082 kb on + strand at 951.082 kb on + strand at 951.082 kb on + strand at 951.082 kb on + strand at 951.082 kb on + strand at 951.085 kb on + strand at 951.086 kb on - strand at 951.099 kb on - strand at 951.099 kb on - strand at 951.176 kb on - strand at 951.290 kb on + strand, within PFLU_RS04195 at 951.290 kb on + strand, within PFLU_RS04195 at 951.290 kb on + strand, within PFLU_RS04195 at 951.504 kb on - strand, within PFLU_RS04195 at 951.524 kb on + strand, within PFLU_RS04195 at 951.524 kb on + strand, within PFLU_RS04195 at 951.606 kb on + strand, within PFLU_RS04195 at 951.627 kb on + strand, within PFLU_RS04195 at 951.627 kb on + strand, within PFLU_RS04195 at 951.627 kb on + strand, within PFLU_RS04195 at 951.628 kb on - strand, within PFLU_RS04195 at 951.675 kb on + strand at 951.675 kb on + strand at 951.675 kb on + strand at 951.676 kb on - strand at 951.676 kb on - strand at 951.676 kb on - strand at 951.676 kb on - strand at 951.797 kb on + strand at 951.798 kb on - strand at 951.798 kb on - strand at 951.800 kb on + strand at 951.827 kb on + strand at 951.827 kb on + strand at 951.827 kb on + strand at 951.827 kb on + strand at 951.827 kb on + strand at 951.828 kb on - strand at 951.899 kb on + strand, within PFLU_RS04200 at 951.899 kb on + strand, within PFLU_RS04200 at 951.899 kb on + strand, within PFLU_RS04200 at 951.899 kb on + strand, within PFLU_RS04200 at 951.899 kb on + strand, within PFLU_RS04200 at 951.899 kb on + strand, within PFLU_RS04200 at 951.899 kb on + strand, within PFLU_RS04200 at 951.899 kb on + strand, within PFLU_RS04200 at 951.899 kb on + strand, within PFLU_RS04200 at 951.899 kb on + strand, within PFLU_RS04200 at 951.899 kb on + strand, within PFLU_RS04200 at 951.899 kb on + strand, within PFLU_RS04200 at 951.899 kb on + strand, within PFLU_RS04200 at 951.900 kb on - strand, within PFLU_RS04200 at 951.900 kb on - strand, within PFLU_RS04200 at 951.900 kb on - strand, within PFLU_RS04200 at 951.900 kb on - strand, within PFLU_RS04200 at 951.900 kb on - strand, within PFLU_RS04200 at 951.900 kb on - strand, within PFLU_RS04200 at 951.900 kb on - strand, within PFLU_RS04200 at 951.900 kb on - strand, within PFLU_RS04200 at 951.900 kb on - strand, within PFLU_RS04200 at 951.900 kb on - strand, within PFLU_RS04200 at 951.900 kb on - strand, within PFLU_RS04200 at 951.901 kb on + strand, within PFLU_RS04200 at 951.901 kb on + strand, within PFLU_RS04200 at 951.901 kb on + strand, within PFLU_RS04200 at 951.902 kb on - strand, within PFLU_RS04200 at 951.902 kb on - strand, within PFLU_RS04200 at 951.902 kb on - strand, within PFLU_RS04200 at 951.902 kb on - strand, within PFLU_RS04200 at 951.938 kb on + strand, within PFLU_RS04200 at 951.938 kb on + strand, within PFLU_RS04200 at 951.938 kb on + strand, within PFLU_RS04200 at 951.938 kb on + strand, within PFLU_RS04200 at 951.938 kb on + strand, within PFLU_RS04200 at 951.938 kb on + strand, within PFLU_RS04200 at 951.938 kb on + strand, within PFLU_RS04200 at 951.939 kb on - strand, within PFLU_RS04200 at 951.939 kb on - strand, within PFLU_RS04200 at 951.939 kb on - strand, within PFLU_RS04200 at 951.939 kb on - strand, within PFLU_RS04200 at 952.009 kb on + strand, within PFLU_RS04200 at 952.010 kb on - strand, within PFLU_RS04200 at 952.019 kb on + strand, within PFLU_RS04200 at 952.046 kb on + strand, within PFLU_RS04200 at 952.046 kb on + strand, within PFLU_RS04200 at 952.046 kb on + strand, within PFLU_RS04200 at 952.047 kb on - strand, within PFLU_RS04200 at 952.100 kb on + strand, within PFLU_RS04200 at 952.100 kb on + strand, within PFLU_RS04200 at 952.101 kb on - strand, within PFLU_RS04200 at 952.101 kb on - strand, within PFLU_RS04200 at 952.133 kb on + strand, within PFLU_RS04200 at 952.133 kb on + strand, within PFLU_RS04200 at 952.133 kb on + strand, within PFLU_RS04200 at 952.134 kb on - strand, within PFLU_RS04200 at 952.244 kb on + strand, within PFLU_RS04200 at 952.245 kb on - strand, within PFLU_RS04200 at 952.245 kb on - strand, within PFLU_RS04200 at 952.245 kb on - strand, within PFLU_RS04200 at 952.245 kb on - strand, within PFLU_RS04200 at 952.245 kb on - strand, within PFLU_RS04200 at 952.245 kb on - strand, within PFLU_RS04200 at 952.245 kb on - strand, within PFLU_RS04200 at 952.439 kb on + strand, within PFLU_RS04200 at 952.439 kb on + strand, within PFLU_RS04200 at 952.439 kb on + strand, within PFLU_RS04200 at 952.440 kb on - strand, within PFLU_RS04200 at 952.440 kb on - strand, within PFLU_RS04200 at 952.440 kb on - strand, within PFLU_RS04200 at 952.440 kb on - strand, within PFLU_RS04200 at 952.442 kb on + strand, within PFLU_RS04200 at 952.442 kb on + strand, within PFLU_RS04200 at 952.442 kb on + strand, within PFLU_RS04200 at 952.442 kb on + strand, within PFLU_RS04200 at 952.442 kb on + strand, within PFLU_RS04200 at 952.442 kb on + strand, within PFLU_RS04200 at 952.442 kb on + strand, within PFLU_RS04200 at 952.443 kb on - strand, within PFLU_RS04200 at 952.443 kb on - strand, within PFLU_RS04200 at 952.443 kb on - strand, within PFLU_RS04200 at 952.443 kb on - strand, within PFLU_RS04200 at 952.443 kb on - strand, within PFLU_RS04200 at 952.598 kb on + strand, within PFLU_RS04200 at 952.598 kb on + strand, within PFLU_RS04200 at 952.613 kb on + strand, within PFLU_RS04200 at 952.613 kb on + strand, within PFLU_RS04200
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C); with MOPS remove 950,862 + +0.2 950,862 + -2.1 950,872 + -1.6 950,872 + +0.7 950,872 + +0.8 950,933 + +1.0 950,933 + +1.5 950,933 + -0.2 950,934 - -0.0 950,934 - +0.5 950,935 + +1.0 950,935 + -0.1 950,936 - +0.6 950,979 + +0.1 950,981 + +0.8 950,981 + -0.1 950,981 + +1.4 950,981 + +1.7 950,981 + -0.2 950,981 + -0.9 950,981 + +1.0 950,982 - +1.1 950,982 - +0.8 950,982 - +1.5 950,982 - -1.1 950,982 - +1.0 950,982 - -2.4 950,982 - +1.2 950,984 + +0.2 950,984 + -0.7 950,984 + +2.7 950,984 + +0.5 950,984 + +0.2 950,984 + +0.1 950,985 - +1.4 950,985 - +1.0 950,985 - -0.3 950,985 - +1.1 950,985 - +1.4 950,985 - -0.6 950,985 - +0.8 951,025 + +0.9 951,025 + +0.9 951,025 + +1.1 951,026 - -0.0 951,026 - -0.1 951,026 - -0.2 951,026 - +0.1 951,026 - +0.2 951,043 - +0.6 951,043 - -0.7 951,063 + -0.6 951,064 - +1.7 951,067 + -1.8 951,067 + -2.8 951,067 + +0.9 951,067 + -0.5 951,067 + +1.7 951,068 - +0.5 951,068 - +2.5 951,068 - -0.2 951,068 - +1.1 951,068 - +0.1 951,068 - -0.7 951,068 - +3.1 951,068 - +0.2 951,082 + -1.8 951,082 + +1.4 951,082 + +0.1 951,082 + -1.1 951,082 + -0.7 951,082 + +0.1 951,082 + +0.5 951,085 + +0.0 951,086 - -0.7 951,099 - -1.7 951,099 - +0.4 951,176 - +3.7 951,290 + PFLU_RS04195 0.27 +1.2 951,290 + PFLU_RS04195 0.27 +1.5 951,290 + PFLU_RS04195 0.27 -1.1 951,504 - PFLU_RS04195 0.63 -1.7 951,524 + PFLU_RS04195 0.66 +1.5 951,524 + PFLU_RS04195 0.66 +0.8 951,606 + PFLU_RS04195 0.80 +0.2 951,627 + PFLU_RS04195 0.83 -1.1 951,627 + PFLU_RS04195 0.83 -0.5 951,627 + PFLU_RS04195 0.83 -0.6 951,628 - PFLU_RS04195 0.84 +1.8 951,675 + +1.5 951,675 + -0.7 951,675 + +0.7 951,676 - +0.1 951,676 - +0.2 951,676 - +0.3 951,676 - +1.4 951,797 + +0.5 951,798 - +1.4 951,798 - -1.4 951,800 + +0.9 951,827 + -1.2 951,827 + +0.6 951,827 + -0.9 951,827 + +0.2 951,827 + +0.0 951,828 - +0.2 951,899 + PFLU_RS04200 0.14 -1.4 951,899 + PFLU_RS04200 0.14 +1.1 951,899 + PFLU_RS04200 0.14 +0.1 951,899 + PFLU_RS04200 0.14 +1.5 951,899 + PFLU_RS04200 0.14 +1.8 951,899 + PFLU_RS04200 0.14 +1.2 951,899 + PFLU_RS04200 0.14 -0.0 951,899 + PFLU_RS04200 0.14 +0.8 951,899 + PFLU_RS04200 0.14 -0.1 951,899 + PFLU_RS04200 0.14 +0.4 951,899 + PFLU_RS04200 0.14 -0.7 951,899 + PFLU_RS04200 0.14 -0.1 951,899 + PFLU_RS04200 0.14 +2.5 951,900 - PFLU_RS04200 0.14 +0.3 951,900 - PFLU_RS04200 0.14 +1.5 951,900 - PFLU_RS04200 0.14 -0.7 951,900 - PFLU_RS04200 0.14 -0.3 951,900 - PFLU_RS04200 0.14 -1.6 951,900 - PFLU_RS04200 0.14 +0.5 951,900 - PFLU_RS04200 0.14 +1.5 951,900 - PFLU_RS04200 0.14 +0.5 951,900 - PFLU_RS04200 0.14 -0.6 951,900 - PFLU_RS04200 0.14 +1.7 951,900 - PFLU_RS04200 0.14 -1.6 951,901 + PFLU_RS04200 0.15 +0.4 951,901 + PFLU_RS04200 0.15 -0.2 951,901 + PFLU_RS04200 0.15 +1.6 951,902 - PFLU_RS04200 0.15 +0.7 951,902 - PFLU_RS04200 0.15 -0.5 951,902 - PFLU_RS04200 0.15 +0.3 951,902 - PFLU_RS04200 0.15 -1.4 951,938 + PFLU_RS04200 0.18 +2.5 951,938 + PFLU_RS04200 0.18 +1.5 951,938 + PFLU_RS04200 0.18 +1.4 951,938 + PFLU_RS04200 0.18 -2.0 951,938 + PFLU_RS04200 0.18 -0.5 951,938 + PFLU_RS04200 0.18 -0.5 951,938 + PFLU_RS04200 0.18 -0.4 951,939 - PFLU_RS04200 0.18 +2.5 951,939 - PFLU_RS04200 0.18 -1.8 951,939 - PFLU_RS04200 0.18 +0.1 951,939 - PFLU_RS04200 0.18 -0.7 952,009 + PFLU_RS04200 0.23 +0.5 952,010 - PFLU_RS04200 0.24 +0.3 952,019 + PFLU_RS04200 0.24 +3.1 952,046 + PFLU_RS04200 0.26 +1.0 952,046 + PFLU_RS04200 0.26 +0.3 952,046 + PFLU_RS04200 0.26 -0.2 952,047 - PFLU_RS04200 0.27 -0.4 952,100 + PFLU_RS04200 0.31 -0.7 952,100 + PFLU_RS04200 0.31 +0.7 952,101 - PFLU_RS04200 0.31 +0.6 952,101 - PFLU_RS04200 0.31 +0.8 952,133 + PFLU_RS04200 0.34 -1.4 952,133 + PFLU_RS04200 0.34 +0.8 952,133 + PFLU_RS04200 0.34 +0.8 952,134 - PFLU_RS04200 0.34 -0.7 952,244 + PFLU_RS04200 0.43 +0.2 952,245 - PFLU_RS04200 0.43 -0.4 952,245 - PFLU_RS04200 0.43 +0.1 952,245 - PFLU_RS04200 0.43 +0.5 952,245 - PFLU_RS04200 0.43 -0.2 952,245 - PFLU_RS04200 0.43 -0.2 952,245 - PFLU_RS04200 0.43 +1.1 952,245 - PFLU_RS04200 0.43 -1.1 952,439 + PFLU_RS04200 0.59 +0.5 952,439 + PFLU_RS04200 0.59 -0.1 952,439 + PFLU_RS04200 0.59 -1.0 952,440 - PFLU_RS04200 0.59 +2.1 952,440 - PFLU_RS04200 0.59 +0.8 952,440 - PFLU_RS04200 0.59 +0.5 952,440 - PFLU_RS04200 0.59 +1.5 952,442 + PFLU_RS04200 0.59 +2.1 952,442 + PFLU_RS04200 0.59 -0.5 952,442 + PFLU_RS04200 0.59 -0.5 952,442 + PFLU_RS04200 0.59 +0.8 952,442 + PFLU_RS04200 0.59 +1.4 952,442 + PFLU_RS04200 0.59 +0.4 952,442 + PFLU_RS04200 0.59 +0.1 952,443 - PFLU_RS04200 0.59 +1.8 952,443 - PFLU_RS04200 0.59 -1.5 952,443 - PFLU_RS04200 0.59 -0.3 952,443 - PFLU_RS04200 0.59 -0.2 952,443 - PFLU_RS04200 0.59 +1.6 952,598 + PFLU_RS04200 0.72 +0.1 952,598 + PFLU_RS04200 0.72 -1.8 952,613 + PFLU_RS04200 0.73 +0.4 952,613 + PFLU_RS04200 0.73 +2.0
Or see this region's nucleotide sequence