Experiment: Trisodium citrate (C); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS03915 and PFLU_RS03920 are separated by 104 nucleotides PFLU_RS03920 and PFLU_RS03925 are separated by 75 nucleotides PFLU_RS03925 and PFLU_RS03930 overlap by 4 nucleotides PFLU_RS03930 and PFLU_RS03935 overlap by 4 nucleotides PFLU_RS03935 and PFLU_RS03940 overlap by 4 nucleotides
PFLU_RS03915: PFLU_RS03915 - DUF1780 domain-containing protein, at 887,494 to 888,123
_RS03915
PFLU_RS03920: PFLU_RS03920 - membrane protein, at 888,228 to 888,917
_RS03920
PFLU_RS03925: PFLU_RS03925 - hypothetical protein, at 888,993 to 889,208
_RS03925
PFLU_RS03930: PFLU_RS03930 - DNA gyrase inhibitor YacG, at 889,205 to 889,405
_RS03930
PFLU_RS03935: PFLU_RS03935 - dephospho-CoA kinase, at 889,402 to 890,025
_RS03935
PFLU_RS03940: PFLU_RS03940 - prepilin peptidase, at 890,022 to 890,888
_RS03940
Position (kb) 888
889
890 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 888.108 kb on - strand at 888.108 kb on - strand at 888.108 kb on - strand at 888.123 kb on + strand at 888.123 kb on + strand at 888.123 kb on + strand at 888.123 kb on + strand at 888.123 kb on + strand at 888.123 kb on + strand at 888.123 kb on + strand at 888.123 kb on + strand at 888.123 kb on + strand at 888.123 kb on + strand at 888.124 kb on - strand at 888.124 kb on - strand at 888.124 kb on - strand at 888.124 kb on - strand at 888.124 kb on - strand at 888.124 kb on - strand at 888.124 kb on - strand at 888.127 kb on + strand at 888.127 kb on + strand at 888.127 kb on + strand at 888.128 kb on - strand at 888.128 kb on - strand at 888.128 kb on - strand at 888.128 kb on - strand at 888.128 kb on - strand at 888.128 kb on - strand at 888.136 kb on - strand at 888.162 kb on + strand at 888.162 kb on + strand at 888.163 kb on - strand at 888.163 kb on - strand at 888.163 kb on - strand at 888.163 kb on - strand at 888.183 kb on + strand at 888.183 kb on + strand at 888.200 kb on + strand at 888.201 kb on - strand at 888.222 kb on - strand at 888.282 kb on + strand at 888.282 kb on + strand at 888.282 kb on + strand at 888.282 kb on + strand at 888.282 kb on + strand at 888.282 kb on + strand at 888.282 kb on + strand at 888.282 kb on + strand at 888.282 kb on + strand at 888.282 kb on + strand at 888.282 kb on + strand at 888.283 kb on - strand at 888.283 kb on - strand at 888.283 kb on - strand at 888.283 kb on - strand at 888.283 kb on - strand at 888.283 kb on - strand at 888.400 kb on + strand, within PFLU_RS03920 at 888.417 kb on - strand, within PFLU_RS03920 at 888.429 kb on + strand, within PFLU_RS03920 at 888.429 kb on + strand, within PFLU_RS03920 at 888.430 kb on - strand, within PFLU_RS03920 at 888.430 kb on - strand, within PFLU_RS03920 at 888.430 kb on - strand, within PFLU_RS03920 at 888.430 kb on - strand, within PFLU_RS03920 at 888.430 kb on - strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.645 kb on + strand, within PFLU_RS03920 at 888.646 kb on - strand, within PFLU_RS03920 at 888.646 kb on - strand, within PFLU_RS03920 at 888.646 kb on - strand, within PFLU_RS03920 at 888.646 kb on - strand, within PFLU_RS03920 at 888.646 kb on - strand, within PFLU_RS03920 at 888.646 kb on - strand, within PFLU_RS03920 at 888.646 kb on - strand, within PFLU_RS03920 at 888.646 kb on - strand, within PFLU_RS03920 at 888.646 kb on - strand, within PFLU_RS03920 at 888.646 kb on - strand, within PFLU_RS03920 at 888.646 kb on - strand, within PFLU_RS03920 at 888.646 kb on - strand, within PFLU_RS03920 at 888.646 kb on - strand, within PFLU_RS03920 at 888.646 kb on - strand, within PFLU_RS03920 at 888.646 kb on - strand, within PFLU_RS03920 at 888.646 kb on - strand, within PFLU_RS03920 at 888.647 kb on + strand, within PFLU_RS03920 at 888.647 kb on + strand, within PFLU_RS03920 at 888.647 kb on + strand, within PFLU_RS03920 at 888.647 kb on + strand, within PFLU_RS03920 at 888.647 kb on + strand, within PFLU_RS03920 at 888.647 kb on + strand, within PFLU_RS03920 at 888.647 kb on + strand, within PFLU_RS03920 at 888.648 kb on - strand, within PFLU_RS03920 at 888.648 kb on - strand, within PFLU_RS03920 at 888.648 kb on - strand, within PFLU_RS03920 at 888.648 kb on - strand, within PFLU_RS03920 at 888.648 kb on - strand, within PFLU_RS03920 at 888.648 kb on - strand, within PFLU_RS03920 at 888.700 kb on + strand, within PFLU_RS03920 at 888.700 kb on + strand, within PFLU_RS03920 at 888.701 kb on - strand, within PFLU_RS03920 at 888.701 kb on - strand, within PFLU_RS03920 at 888.701 kb on - strand, within PFLU_RS03920 at 888.703 kb on + strand, within PFLU_RS03920 at 888.704 kb on - strand, within PFLU_RS03920 at 888.704 kb on - strand, within PFLU_RS03920 at 888.704 kb on - strand, within PFLU_RS03920 at 888.704 kb on - strand, within PFLU_RS03920 at 888.704 kb on - strand, within PFLU_RS03920 at 888.771 kb on + strand, within PFLU_RS03920 at 888.771 kb on + strand, within PFLU_RS03920 at 888.772 kb on - strand, within PFLU_RS03920 at 888.877 kb on - strand at 888.971 kb on - strand at 889.002 kb on + strand at 889.002 kb on + strand at 889.002 kb on + strand at 889.002 kb on + strand at 889.002 kb on + strand at 889.002 kb on + strand at 889.003 kb on - strand at 889.003 kb on - strand at 889.003 kb on - strand at 889.003 kb on - strand at 889.003 kb on - strand at 889.003 kb on - strand at 889.003 kb on - strand at 889.003 kb on - strand at 889.003 kb on - strand at 889.003 kb on - strand at 889.155 kb on + strand, within PFLU_RS03925 at 889.155 kb on + strand, within PFLU_RS03925 at 889.155 kb on + strand, within PFLU_RS03925 at 889.155 kb on + strand, within PFLU_RS03925 at 889.156 kb on - strand, within PFLU_RS03925 at 889.156 kb on - strand, within PFLU_RS03925 at 889.188 kb on + strand at 889.188 kb on + strand at 889.188 kb on + strand at 889.188 kb on + strand at 889.188 kb on + strand at 889.188 kb on + strand at 889.188 kb on + strand at 889.189 kb on - strand at 889.189 kb on - strand at 889.189 kb on - strand at 889.196 kb on + strand at 889.196 kb on + strand at 889.206 kb on + strand at 889.206 kb on + strand at 889.207 kb on - strand at 889.207 kb on - strand at 889.207 kb on - strand at 889.270 kb on + strand, within PFLU_RS03930 at 889.270 kb on + strand, within PFLU_RS03930 at 889.270 kb on + strand, within PFLU_RS03930 at 889.271 kb on - strand, within PFLU_RS03930 at 889.271 kb on - strand, within PFLU_RS03930 at 889.271 kb on - strand, within PFLU_RS03930 at 889.271 kb on - strand, within PFLU_RS03930 at 889.271 kb on - strand, within PFLU_RS03930 at 889.271 kb on - strand, within PFLU_RS03930 at 889.271 kb on - strand, within PFLU_RS03930 at 889.271 kb on - strand, within PFLU_RS03930 at 889.271 kb on - strand, within PFLU_RS03930 at 889.271 kb on - strand, within PFLU_RS03930 at 889.271 kb on - strand, within PFLU_RS03930 at 889.271 kb on - strand, within PFLU_RS03930 at 889.271 kb on - strand, within PFLU_RS03930 at 889.425 kb on + strand at 889.425 kb on + strand at 889.425 kb on + strand at 889.426 kb on - strand at 889.426 kb on - strand at 889.426 kb on - strand at 889.426 kb on - strand at 889.426 kb on - strand at 889.426 kb on - strand at 889.426 kb on - strand at 890.047 kb on + strand at 890.047 kb on + strand at 890.048 kb on - strand at 890.048 kb on - strand at 890.048 kb on - strand at 890.104 kb on + strand at 890.104 kb on + strand at 890.104 kb on + strand at 890.104 kb on + strand at 890.105 kb on - strand at 890.191 kb on - strand, within PFLU_RS03940
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C); with MOPS remove 888,108 - -0.6 888,108 - -0.2 888,108 - +2.1 888,123 + -0.1 888,123 + +0.4 888,123 + +0.5 888,123 + +0.1 888,123 + +0.4 888,123 + +0.0 888,123 + -2.8 888,123 + +0.6 888,123 + +0.2 888,123 + -0.4 888,124 - +1.0 888,124 - +1.5 888,124 - -0.5 888,124 - +0.7 888,124 - +0.5 888,124 - -0.2 888,124 - +0.7 888,127 + +1.5 888,127 + -0.1 888,127 + +0.9 888,128 - -0.4 888,128 - +0.1 888,128 - -0.1 888,128 - +2.8 888,128 - +2.3 888,128 - +0.1 888,136 - +1.6 888,162 + -0.7 888,162 + -0.5 888,163 - +0.2 888,163 - +1.1 888,163 - -1.0 888,163 - +0.9 888,183 + +0.4 888,183 + +0.2 888,200 + -0.1 888,201 - -0.1 888,222 - -1.0 888,282 + +1.0 888,282 + -0.9 888,282 + -2.0 888,282 + +0.9 888,282 + -0.7 888,282 + -0.4 888,282 + +1.3 888,282 + +0.9 888,282 + -0.7 888,282 + +0.1 888,282 + -0.2 888,283 - +0.5 888,283 - +0.3 888,283 - -0.0 888,283 - +0.5 888,283 - +1.7 888,283 - -0.7 888,400 + PFLU_RS03920 0.25 -0.2 888,417 - PFLU_RS03920 0.27 +1.2 888,429 + PFLU_RS03920 0.29 -0.4 888,429 + PFLU_RS03920 0.29 +0.6 888,430 - PFLU_RS03920 0.29 +0.7 888,430 - PFLU_RS03920 0.29 +0.3 888,430 - PFLU_RS03920 0.29 -1.3 888,430 - PFLU_RS03920 0.29 +0.8 888,430 - PFLU_RS03920 0.29 +1.1 888,645 + PFLU_RS03920 0.60 +1.4 888,645 + PFLU_RS03920 0.60 +0.8 888,645 + PFLU_RS03920 0.60 +0.2 888,645 + PFLU_RS03920 0.60 -0.2 888,645 + PFLU_RS03920 0.60 -0.6 888,645 + PFLU_RS03920 0.60 -1.3 888,645 + PFLU_RS03920 0.60 +1.7 888,645 + PFLU_RS03920 0.60 +0.6 888,645 + PFLU_RS03920 0.60 -0.0 888,645 + PFLU_RS03920 0.60 +0.5 888,645 + PFLU_RS03920 0.60 +0.1 888,645 + PFLU_RS03920 0.60 +1.0 888,645 + PFLU_RS03920 0.60 +0.6 888,645 + PFLU_RS03920 0.60 +1.7 888,645 + PFLU_RS03920 0.60 +0.1 888,645 + PFLU_RS03920 0.60 +0.5 888,645 + PFLU_RS03920 0.60 +0.2 888,645 + PFLU_RS03920 0.60 -0.5 888,645 + PFLU_RS03920 0.60 -0.1 888,646 - PFLU_RS03920 0.61 -0.4 888,646 - PFLU_RS03920 0.61 -0.8 888,646 - PFLU_RS03920 0.61 +1.1 888,646 - PFLU_RS03920 0.61 -2.1 888,646 - PFLU_RS03920 0.61 -0.5 888,646 - PFLU_RS03920 0.61 -1.6 888,646 - PFLU_RS03920 0.61 -0.4 888,646 - PFLU_RS03920 0.61 +1.4 888,646 - PFLU_RS03920 0.61 +1.4 888,646 - PFLU_RS03920 0.61 +1.1 888,646 - PFLU_RS03920 0.61 +0.1 888,646 - PFLU_RS03920 0.61 -1.6 888,646 - PFLU_RS03920 0.61 +0.6 888,646 - PFLU_RS03920 0.61 -0.3 888,646 - PFLU_RS03920 0.61 +0.1 888,646 - PFLU_RS03920 0.61 +1.2 888,647 + PFLU_RS03920 0.61 -0.6 888,647 + PFLU_RS03920 0.61 +0.2 888,647 + PFLU_RS03920 0.61 +1.0 888,647 + PFLU_RS03920 0.61 +0.8 888,647 + PFLU_RS03920 0.61 -0.8 888,647 + PFLU_RS03920 0.61 -0.1 888,647 + PFLU_RS03920 0.61 -0.8 888,648 - PFLU_RS03920 0.61 +0.7 888,648 - PFLU_RS03920 0.61 -1.8 888,648 - PFLU_RS03920 0.61 +0.7 888,648 - PFLU_RS03920 0.61 -0.8 888,648 - PFLU_RS03920 0.61 +0.1 888,648 - PFLU_RS03920 0.61 -0.9 888,700 + PFLU_RS03920 0.68 +0.4 888,700 + PFLU_RS03920 0.68 -0.8 888,701 - PFLU_RS03920 0.69 +1.5 888,701 - PFLU_RS03920 0.69 +0.2 888,701 - PFLU_RS03920 0.69 +0.2 888,703 + PFLU_RS03920 0.69 +0.1 888,704 - PFLU_RS03920 0.69 +0.1 888,704 - PFLU_RS03920 0.69 -0.6 888,704 - PFLU_RS03920 0.69 -1.0 888,704 - PFLU_RS03920 0.69 +1.0 888,704 - PFLU_RS03920 0.69 -2.1 888,771 + PFLU_RS03920 0.79 +0.1 888,771 + PFLU_RS03920 0.79 -2.6 888,772 - PFLU_RS03920 0.79 -0.5 888,877 - +0.7 888,971 - -1.6 889,002 + +0.7 889,002 + -0.2 889,002 + +1.5 889,002 + +0.5 889,002 + -0.9 889,002 + +0.1 889,003 - -0.7 889,003 - -1.0 889,003 - -1.6 889,003 - +0.1 889,003 - +0.7 889,003 - +1.0 889,003 - +0.0 889,003 - -2.1 889,003 - +0.8 889,003 - +2.1 889,155 + PFLU_RS03925 0.75 +0.6 889,155 + PFLU_RS03925 0.75 +0.7 889,155 + PFLU_RS03925 0.75 -0.5 889,155 + PFLU_RS03925 0.75 +0.2 889,156 - PFLU_RS03925 0.75 +0.9 889,156 - PFLU_RS03925 0.75 +0.9 889,188 + +1.2 889,188 + -3.8 889,188 + -0.0 889,188 + -0.4 889,188 + +0.4 889,188 + -0.7 889,188 + -1.8 889,189 - +0.4 889,189 - -0.5 889,189 - -2.4 889,196 + +0.9 889,196 + -1.3 889,206 + +0.5 889,206 + -0.1 889,207 - +0.6 889,207 - -1.3 889,207 - -2.3 889,270 + PFLU_RS03930 0.32 -0.9 889,270 + PFLU_RS03930 0.32 -0.3 889,270 + PFLU_RS03930 0.32 -0.1 889,271 - PFLU_RS03930 0.33 +0.9 889,271 - PFLU_RS03930 0.33 +0.0 889,271 - PFLU_RS03930 0.33 +0.1 889,271 - PFLU_RS03930 0.33 -1.0 889,271 - PFLU_RS03930 0.33 +0.4 889,271 - PFLU_RS03930 0.33 -0.4 889,271 - PFLU_RS03930 0.33 +0.9 889,271 - PFLU_RS03930 0.33 -1.0 889,271 - PFLU_RS03930 0.33 +0.7 889,271 - PFLU_RS03930 0.33 +0.8 889,271 - PFLU_RS03930 0.33 -0.0 889,271 - PFLU_RS03930 0.33 +0.7 889,271 - PFLU_RS03930 0.33 -0.2 889,425 + -1.1 889,425 + -1.3 889,425 + -0.2 889,426 - +0.4 889,426 - -1.5 889,426 - +0.7 889,426 - -0.7 889,426 - -0.6 889,426 - +0.6 889,426 - -0.3 890,047 + +0.7 890,047 + -2.3 890,048 - +0.3 890,048 - -0.3 890,048 - -0.6 890,104 + +1.1 890,104 + -0.2 890,104 + +0.0 890,104 + +0.4 890,105 - +0.0 890,191 - PFLU_RS03940 0.19 -2.1
Or see this region's nucleotide sequence